Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77270-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77323-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77273-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77208-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76740SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77311-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77005-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76996-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76728SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76586-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77089-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76588-0.025mg | |||
![Western Blot: Nicastrin Antibody [NBP1-77270] - Human brain tissue lysate with Nicastrin antibody at (A) 0.5 and (B) 1ug/ml.](http://images.novusbio.com/fullsize/Nicastrin-Antibody-Western-Blot-NBP1-77270-img0002.jpg "Western Blot: Nicastrin Antibody [NBP1-77270] - Human brain tissue lysate with Nicastrin antibody at (A) 0.5 and (B) 1ug/ml.")
![Immunohistochemistry: Nicastrin Antibody - BSA Free [NBP1-77270] - Immunohistochemistry of Nicastrin in human brain tissue with Nicastrin antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77270_rabbit-polyclonal-nicastrin-antibody-271120241928886.jpg)
![Immunocytochemistry/ Immunofluorescence: Nicastrin Antibody - BSA Free [NBP1-77270] - Immunofluorescence of Nicastrin in mouse brain tissue with Nicastrin antibody at 20 ug/ml.Green: Nicastrin Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-77270_rabbit-polyclonal-nicastrin-antibody-2711202420141926.gif)
![Immunocytochemistry/ Immunofluorescence: Nicastrin Antibody - BSA Free [NBP1-77270] - Immunofluorescence of Nicastrin in Human Brain cells with Nicastrin antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77270_rabbit-polyclonal-nicastrin-antibody-2711202419333434.jpg)
![Western Blot: CADPS Antibody [NBP1-77323] - Rat brain tissue lysate with CADPS antibody at (A) 0.5 and (B) 1 ug/ml.](http://images.novusbio.com/fullsize/CADPS-Antibody-Western-Blot-NBP1-77323-img0001.jpg "Western Blot: CADPS Antibody [NBP1-77323] - Rat brain tissue lysate with CADPS antibody at (A) 0.5 and (B) 1 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: CADPS Antibody - BSA Free [NBP1-77323] - Immunofluorescence of CADPS in human brain tissue with CADPS antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77323_rabbit-polyclonal-cadps-antibody-2711202419201575.jpg)
![Immunohistochemistry: CADPS Antibody - BSA Free [NBP1-77323] - Immunohistochemistry of CADPS in human brain with CADPS antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77323_rabbit-polyclonal-cadps-antibody-2711202419541552.jpg)
![Western Blot: SCO1 Antibody [NBP1-77273] - Human brain tissue lysate with SCO1 antibody at (A) 0.5 and (B) 1 ug/mL.](http://images.novusbio.com/fullsize/SCO1-Antibody-Western-Blot-NBP1-77273-img0001.jpg "Western Blot: SCO1 Antibody [NBP1-77273] - Human brain tissue lysate with SCO1 antibody at (A) 0.5 and (B) 1 ug/mL.")
![Immunohistochemistry: SCO1 Antibody - BSA Free [NBP1-77273] - Immunohistochemistry of SCO1 in human brain tissue with SCO1 antibody at 2.5 u/mL.](http://images.novusbio.com/fullsize/nbp1-77273_rabbit-polyclonal-sco1-antibody-271120241923695.jpg)
![Immunocytochemistry/ Immunofluorescence: SCO1 Antibody - BSA Free [NBP1-77273] - Immunofluorescence of SCO1 in Human Brain cells with SCO1 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77273_rabbit-polyclonal-sco1-antibody-271120242013199.jpg)
![Western Blot: Caspase-4 Antibody [NBP1-77208] - Human spleen cells with caspase-4 antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/Caspase-4-Antibody-Western-Blot-NBP1-77208-img0001.jpg "Western Blot: Caspase-4 Antibody [NBP1-77208] - Human spleen cells with caspase-4 antibody at (A) 1 and (B) 2 ug/ml.")
![Immunohistochemistry-Paraffin: Caspase-4 Antibody [NBP1-77208] - Human spleen tissue using caspase-4 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Caspase-4-Antibody-Immunohistochemistry-Paraffin-NBP1-77208-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Caspase-4 Antibody - BSA Free [NBP1-77208] - Immunofluorescence of Caspase-4 in A20 cells with Caspase-4 antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77208_rabbit-polyclonal-caspase-4-antibody-2711202419475865.jpg)
![Western Blot: IL-21 Antibody [NBP1-76740] - Western Blot Validation with Recombinant Protein.Loading: 30 ng of human IL-21 recombinant protein per lane.Antibodies: IL-21 NBP1-76740 (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/IL-21-Antibody-Western-Blot-NBP1-76740-img0007.jpg "<b>Expression of Tagged Proteins Validation. </b>Western Blot: IL-21 Antibody [NBP1-76740] - Western Blot Validation with Recombinant Protein.Loading: 30 ng of human IL-21 recombinant protein per lane.Antibodies: IL-21 NBP1-76740 (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Expression of Tagged Proteins Validation. </b>Immunocytochemistry/Immunofluorescence: IL-21 Antibody [NBP1-76740] - Immunocytochemistry Validation of IL-21 in K562 Cells.Immunocytochemical analysis of K562 cells using anti-IL-21 antibody (NBP1-76740) at 2 ug/mL. Cells were fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/IL-21-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76740-img0008.jpg)
![Western Blot: IL-21 Antibody [NBP1-76740] - Western Blot Validation in Human, Mouse and Rat Cell Lines.Loading: 15 ug of lysates per lane.Antibodies: IL-21 NBP1-76740, (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/IL-21-Antibody-Western-Blot-NBP1-76740-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: IL-21 Antibody [NBP1-76740] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines.Loading: 15 ug of lysates per lane.Antibodies: IL-21 NBP1-76740 (1 ug/mL), IL-21 from Company B (5 ug/mL), beta-actin (5 ug/mL) and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/IL-21-Antibody-Western-Blot-NBP1-76740-img0005.jpg)
![Western Blot: IL-21 Antibody - BSA Free [NBP1-76740] - Figure 3 Western Blot Validation in Human HL-60 Cell LysateLoading: 15 u of lysates per lane.Antibodies: IL-21 (1 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.(A) Absence of blocking peptide(B) Presence of blocking peptide.](http://images.novusbio.com/fullsize/nbp1-76740_rabbit-polyclonal-il-21-antibody-271120241937752.jpg)
![Western Blot: Endosialin/CD248/TEM1 Antibody [NBP1-77311] - Western Blot Validation in Human TissueLoading: 15 ug of lysates per lane.Antibodies: TEM-1 NBP1-77311, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Endosialin-CD248-TEM1-Antibody-Western-Blot-NBP1-77311-img0005.jpg "Western Blot: Endosialin/CD248/TEM1 Antibody [NBP1-77311] - Western Blot Validation in Human TissueLoading: 15 ug of lysates per lane.Antibodies: TEM-1 NBP1-77311, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Western Blot: Endosialin/CD248/TEM1 Antibody [NBP1-77311] - Human colon tissue lysate with TEM1 antibody at (A) 0.5 and (B) 1 ug/ml.](http://images.novusbio.com/fullsize/Endosialin-CD248-TEM1-Antibody-Western-Blot-NBP1-77311-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Endosialin/CD248/TEM1 Antibody [NBP1-77311] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 ug of lysates per lane.Antibodies: TEM-1 NBP1-77310, (2 ug/mL), TEM-1 NBP1-77311, ( 1ug/mL), and GAPDH (0.02 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Endosialin-CD248-TEM1-Antibody-Western-Blot-NBP1-77311-img0004.jpg)
![Immunohistochemistry: Endosialin/CD248/TEM1 Antibody - BSA Free [NBP1-77311] - Figure 4 Immunohistochemistry Validation of TEM-1 in Human Colon Tissue Immunohistochemical analysis of paraffin-embedded Human Colon tissue using anti-TEM-1 antibody at 2.5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77311_rabbit-polyclonal-endosialin-cd248-tem1-antibody-271120241932644.jpg)
![Immunocytochemistry/ Immunofluorescence: Endosialin/CD248/TEM1 Antibody - BSA Free [NBP1-77311] - Validation of TEM-1 in Human Colon Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Colon Tissue labeling TEM-1 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-77311_rabbit-polyclonal-endosialin-cd248-tem1-antibody-271120242004185.jpg)
![Western Blot: Protocadherin-18 Antibody [NBP1-77005] - Analysis in HepG2 cell lysate with antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/Protocadherin-18-Antibody-Western-Blot-NBP1-77005-img0004.jpg "Western Blot: Protocadherin-18 Antibody [NBP1-77005] - Analysis in HepG2 cell lysate with antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.")
![Western Blot: Protocadherin-18 Antibody [NBP1-77005] - Raji cell lysate with PCDH18 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Protocadherin-18-Antibody-Western-Blot-NBP1-77005-img0002.jpg)
![Immunohistochemistry: Protocadherin-18 Antibody - BSA Free [NBP1-77005] - Immunohistochemistry of Protocadherin-18 in mouse brain tissue with Protocadherin-18 antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77005_rabbit-polyclonal-protocadherin-18-antibody-271120242013136.jpg)
![Immunocytochemistry/ Immunofluorescence: Protocadherin-18 Antibody - BSA Free [NBP1-77005] - Immunofluorescence of Protocadherin-18 in Mouse Brain cells with Protocadherin-18 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77005_rabbit-polyclonal-protocadherin-18-antibody-271120241923647.jpg)
![Western Blot: CDIP Antibody [NBP1-76996] - Human brain lysate with CDIP antibody at (A) 0.25 and (B) 0.5 ug/ml.](http://images.novusbio.com/fullsize/CDIP-Antibody-Western-Blot-NBP1-76996-img0002.jpg "Western Blot: CDIP Antibody [NBP1-76996] - Human brain lysate with CDIP antibody at (A) 0.25 and (B) 0.5 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: CDIP Antibody - BSA Free [NBP1-76996] - Immunofluorescence of CDIP1 in mouse brain tissue with CDIP1 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76996_rabbit-polyclonal-cdip-antibody-2711202419172211.jpg)
![Immunohistochemistry: CDIP Antibody - BSA Free [NBP1-76996] - Immunohistochemistry of CDIP in mouse brain tissue with CDIP antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76996_rabbit-polyclonal-cdip-antibody-271120242010588.jpg)
![Western Blot: IL-27R alpha/WSX-1/TCCR Antibody [NBP1-76728] - Analysis of IL27RA expression in human spleen tissue lysates with anti-IL27RA using NBP1-76728 at 1 ug /ml.](http://images.novusbio.com/fullsize/IL-27R-alpha-WSX-1-TCCR-Antibody-Western-Blot-NBP1-76728-img0004.jpg "Western Blot: IL-27R alpha/WSX-1/TCCR Antibody [NBP1-76728] - Analysis of IL27RA expression in human spleen tissue lysates with anti-IL27RA using NBP1-76728 at 1 ug /ml.")
![Immunocytochemistry/ Immunofluorescence: IL-27R alpha/WSX-1/TCCR Antibody - BSA Free [NBP1-76728] - Immunofluorescence of IL-27R alpha/WSX-1/TCCR in A549 cells with IL-27R alpha/WSX-1/TCCR antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-76728_rabbit-polyclonal-il-27r-alpha-wsx-1-tccr-antibody-2711202419201567.jpg)
![Immunohistochemistry: IL-27R alpha/WSX-1/TCCR Antibody - BSA Free [NBP1-76728] - Immunohistochemistry of IL-27R alpha/WSX-1/TCCR in human spleen tissue with IL-27R alpha/WSX-1/TCCR antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76728_rabbit-polyclonal-il-27r-alpha-wsx-1-tccr-antibody-2711202419531667.jpg)
![Western Blot: ATAD3A Antibody [NBP1-76586] - Daudi cell lysate with ATAD3A antibody at 1 ug/mL .](http://images.novusbio.com/fullsize/ATAD3A-Antibody-Western-Blot-NBP1-76586-img0001.jpg "Western Blot: ATAD3A Antibody [NBP1-76586] - Daudi cell lysate with ATAD3A antibody at 1 ug/mL .")
![Immunocytochemistry/Immunofluorescence: ATAD3A Antibody [NBP1-76586] - Immunocytochemistry of ATAD3A in Daudi cells with ATAD3A antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/ATAD3A-Antibody-Immunocytochemistry-NBP1-76586-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: ATAD3A Antibody - BSA Free [NBP1-76586] - Immunofluorescence of ATAD3A in Daudi cells with ATAD3A antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76586_rabbit-polyclonal-atad3a-antibody-2711202420324859.jpg)
![Western Blot: PLEKHM2 Antibody [NBP1-77089] - Rat brain tissue lysate with PLEKHM2 antibody at 0.5 ug/mL in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/PLEKHM2-Antibody-Western-Blot-NBP1-77089-img0001.jpg "Western Blot: PLEKHM2 Antibody [NBP1-77089] - Rat brain tissue lysate with PLEKHM2 antibody at 0.5 ug/mL in (A) the absence and (B) the presence of blocking peptide.")
![Immunohistochemistry: PLEKHM2 Antibody - BSA Free [NBP1-77089] - Immunohistochemistry of PLEKHM2 in human liver tissue with FAM59A antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp1-77089_rabbit-polyclonal-plekhm2-antibody-2711202419592034.jpg)
![Immunocytochemistry/ Immunofluorescence: PLEKHM2 Antibody - BSA Free [NBP1-77089] - Immunofluorescence of PLEKHM2 in Human Brain cells with PLEKHM2 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77089_rabbit-polyclonal-plekhm2-antibody-271120241939275.jpg)
![Western Blot: AP3S1 Antibody [NBP1-76588] - analysis of AP3S1 in mouse kidney tissue lysate with AP3S1 antibody at (A) 1 and (B) 2 ug/mL .](http://images.novusbio.com/fullsize/AP3S1-Antibody-Western-Blot-NBP1-76588-img0001.jpg "Western Blot: AP3S1 Antibody [NBP1-76588] - analysis of AP3S1 in mouse kidney tissue lysate with AP3S1 antibody at (A) 1 and (B) 2 ug/mL .")
![Immunocytochemistry/ Immunofluorescence: AP3S1 Antibody - BSA Free [NBP1-76588] - Immunofluorescence of AP3S1 in human kidney tissue with AP3S1 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76588_rabbit-polyclonal-ap3s1-antibody-27112024192880.jpg)
![Immunohistochemistry: AP3S1 Antibody - BSA Free [NBP1-76588] - Immunohistochemistry of AP3S1 in human kidney tissue with AP3S1 antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76588_rabbit-polyclonal-ap3s1-antibody-2711202420441177.jpg)