Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76837-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77065-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77275-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77361-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36445-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76575-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22340SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41315-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76911-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41263-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41123-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41287-0.025mg | |||
![Western Blot: MANF Antibody [NBP1-76837] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human MANF recombinant protein per lane. Antibodies: MANF NBP1-76837 (Lane 1: 0.125 ug/mL, Lane 2: 0.25 ug/mL and Lane 3: 0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 55kDa.](http://images.novusbio.com/fullsize/MANF-Antibody-Western-Blot-NBP1-76837-img0005.jpg "Western Blot: MANF Antibody [NBP1-76837] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human MANF recombinant protein per lane. Antibodies: MANF NBP1-76837 (Lane 1: 0.125 ug/mL, Lane 2: 0.25 ug/mL and Lane 3: 0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 55kDa.")
![Western Blot: MANF Antibody [NBP1-76837] - Rat brain tissue lysate with ARMET antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/MANF-Antibody-Western-Blot-NBP1-76837-img0001.jpg)
![Western Blot: MANF Antibody [NBP1-76837] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: MANF NBP1-76837, (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/MANF-Antibody-Western-Blot-NBP1-76837-img0004.jpg)
![Immunohistochemistry: MANF Antibody - BSA Free [NBP1-76837] - Validation of MANF in Human Brain Tissue.Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-MANF antibody at 2.5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76837_rabbit-polyclonal-manf-antibody-271120242004148.jpg)
![Immunocytochemistry/ Immunofluorescence: MANF Antibody - BSA Free [NBP1-76837] - Validation of MANF in Human Brain Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human brain tissue labeling MANF with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-76837_rabbit-polyclonal-manf-antibody-271120242042593.jpg)
![Western Blot: CCR3 Antibody [NBP1-77065] - Human spleen tissue lysates with CCR3 antibody at 1 (lane 1) and 2 ug/ml (lane 2), and 2 ug/ml in the presence of blocking peptide (lane 3).](http://images.novusbio.com/fullsize/CCR3-Antibody-Western-Blot-NBP1-77065-img0002.jpg "Western Blot: CCR3 Antibody [NBP1-77065] - Human spleen tissue lysates with CCR3 antibody at 1 (lane 1) and 2 ug/ml (lane 2), and 2 ug/ml in the presence of blocking peptide (lane 3).")
![Immunohistochemistry-Paraffin: CCR3 Antibody [NBP1-77065] - Human spleen tissue with CCR3 antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/CCR3-Antibody-Immunohistochemistry-Paraffin-NBP1-77065-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: CCR3 Antibody - BSA Free [NBP1-77065] - Immunofluorescence of CCR3 in Human Spleen tissue with CCR3 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77065_rabbit-polyclonal-ccr3-antibody-2711202420285882.jpg)
![Western Blot: MAVS Antibody [NBP1-77275] - A20 cell lysate with MAVS antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.](http://images.novusbio.com/fullsize/MAVS-Antibody-Western-Blot-NBP1-77275-img0001.jpg "Western Blot: MAVS Antibody [NBP1-77275] - A20 cell lysate with MAVS antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.")
![Immunohistochemistry-Paraffin: MAVS Antibody [NBP1-77275] - Human brain tissue with MAVS antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/MAVS-Antibody-Immunohistochemistry-Paraffin-NBP1-77275-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: MAVS Antibody - BSA Free [NBP1-77275] - Immunofluorescence of MAVS in Human Brain cells with MAVS antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77275_rabbit-polyclonal-mavs-antibody-2711202420432381.jpg)
![Western Blot: HAAO Antibody [NBP1-77361] - Mouse liver tissue lysate with HAAO antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/HAAO-Antibody-Western-Blot-NBP1-77361-img0005.jpg "Western Blot: HAAO Antibody [NBP1-77361] - Mouse liver tissue lysate with HAAO antibody at 1 ug/mL.")
![Immunohistochemistry: HAAO Antibody - BSA Free [NBP1-77361] - Immunohistochemistry of HAAO in mouse colon tissue with HAAO antibody at 2 u/mL.](http://images.novusbio.com/fullsize/nbp1-77361_rabbit-polyclonal-haao-antibody-2711202420141962.jpg)
![Immunohistochemistry: HAAO Antibody - BSA Free [NBP1-77361] - Immunohistochemistry of HAAO in human liver tissue with HAAO antibody at 2.5 u/mL.](http://images.novusbio.com/fullsize/nbp1-77361_rabbit-polyclonal-haao-antibody-2711202420212388.jpg)
![Immunocytochemistry/ Immunofluorescence: HAAO Antibody - BSA Free [NBP1-77361] - Immunofluorescence of HAAO in Human Liver cells with HAAO antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77361_rabbit-polyclonal-haao-antibody-2711202420272478.jpg)
![Western Blot: ATG14 Antibody - BSA Free [NBP2-36445] - ATG14 Antibody [NBP2-36445] - DHA increases the basal level of autophagy. Protein levels of ATG5, ATG12, ATG16L1, Beclin1, ATG14, p-ATG14 (Ser29), Rab7, RILP, Lamp1, CTSB, p-mTOR(Ser2448), mammalian target of rapamycin (mTOR), ULK1, p-GSK3Beta (Ser9), GSK3Beta in extracts of whole brain tissue were detected by WB. Representative Western blots are presented in panels, and quantification of the results is shown as the mean values +- SD in panels (B,D,F,H). *p less than 0.05, **p less than 0.01, and ***p less than 0.001 between two groups. One-way analysis of variance/Newman-Keuls test was performed for all WB data. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fnagi.2020.00047/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ATG14-Antibody-Western-Blot-NBP2-36445-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: ATG14 Antibody - BSA Free [NBP2-36445] - ATG14 Antibody [NBP2-36445] - DHA increases the basal level of autophagy. Protein levels of ATG5, ATG12, ATG16L1, Beclin1, ATG14, p-ATG14 (Ser29), Rab7, RILP, Lamp1, CTSB, p-mTOR(Ser2448), mammalian target of rapamycin (mTOR), ULK1, p-GSK3Beta (Ser9), GSK3Beta in extracts of whole brain tissue were detected by WB. Representative Western blots are presented in panels, and quantification of the results is shown as the mean values +- SD in panels (B,D,F,H). *p less than 0.05, **p less than 0.01, and ***p less than 0.001 between two groups. One-way analysis of variance/Newman-Keuls test was performed for all WB data. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fnagi.2020.00047/full) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Western Blot: ATG14 Antibody - BSA Free [NBP2-36445] - ATG14 Antibody [NBP2-36445] - Analysis of ATG14 in human small intestine tissue lysate with ATG14 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/ATG14-Antibody-Western-Blot-NBP2-36445-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: ATG14 Antibody - BSA Free [NBP2-36445] - Immunofluorescence of ATG14 in human small intestine tissue with ATG14 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-36445_rabbit-polyclonal-atg14-antibody-2711202420285856.jpg)
![Western Blot: Musashi-2 Antibody [NBP1-76575] - Analysis of MSI2 in EL4 cell lysate with MSI2 antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/Musashi-2-Antibody-Western-Blot-NBP1-76575-img0004.jpg "Western Blot: Musashi-2 Antibody [NBP1-76575] - Analysis of MSI2 in EL4 cell lysate with MSI2 antibody at 1 ug/mL.")
![Immunocytochemistry/Immunofluorescence: Musashi-2 Antibody [NBP1-76575] - EL4 cells with MSI2 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/Musashi-2-Antibody-Immunocytochemistry-NBP1-76575-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Musashi-2 Antibody [NBP1-76575] - EL4 cells with MSI2 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/Musashi-2-Antibody-Immunofluorescence-NBP1-76575-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: Musashi-2 Antibody - BSA Free [NBP1-76575] - Immunofluorescence of Musashi-2 in mouse brain tissue with Musashi-2 Antibody at 20 ug/mL.Green: Musashi-2 antibody Red: Phylloidin staining Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-76575_rabbit-polyclonal-musashi-2-antibody-2711202419241349.gif)
![Immunohistochemistry: Musashi-2 Antibody - BSA Free [NBP1-76575] - Immunohistochemistry of Musashi-2 in mouse brain tissue with Musashi-2 Antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76575_rabbit-polyclonal-musashi-2-antibody-2711202419463426.gif)
![Western Blot: SHISA3 Antibody [NBP2-22340] - Analysis SHISA3 in human small intestine Tissue lysate with SHISA3 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/SHISA3-Antibody-Western-Blot-NBP2-22340-img0002.jpg "Western Blot: SHISA3 Antibody [NBP2-22340] - Analysis SHISA3 in human small intestine Tissue lysate with SHISA3 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.")
![Immunohistochemistry: SHISA3 Antibody [NBP2-22340] - SHISA3 in human small intestine tissue with SHISA3 antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/SHISA3-Antibody-Immunohistochemistry-NBP2-22340-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: SHISA3 Antibody - BSA Free [NBP2-22340] - Immunofluorescence of SHISA3 in human small intestine tissue with SHISA3 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/nbp2-22340_rabbit-polyclonal-shisa3-antibody-2711202419293855.jpg)
![Western Blot: Teneurin-1 Antibody [NBP2-41315] - Western blot analysis of TENM1 in human brain tissue lysate with TENM1 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/Teneurin-1-Antibody-Western-Blot-NBP2-41315-img0001.jpg "Western Blot: Teneurin-1 Antibody [NBP2-41315] - Western blot analysis of TENM1 in human brain tissue lysate with TENM1 antibody at 1 ug/ml.")
![Immunoprecipitation: Teneurin-1 Antibody - BSA Free [NBP2-41315] - HINT1 exhibits isopeptidase activity. (A) HINT1 interactions with the proteins used in the study. (I) HINT1 but not its paralogs forms stable complexes with RGSZ2 proteins. (II) ICD teneurin1 interacts with HINT1 but not with GST. (III, IV) HINT1 associations with RanGAP1 & SP100 were weak or not detected. (B) HINT1 exhibits SUMO protease activity on sumoylated RanGAP1, which is regulated by calcium & CaM. (I) HINT1 (2 μM) but not SENP2 (0.3 μM) requires calcium-activated CaM (6 μM) to exhibit sumoylase activity. (II) Removal of 10 mM MgCl2 from the sumoylation buffer greatly improves Ca2+-CaM-dependent HINT1 isopeptidase activity on sumoylated RanGAP1. (III, IV) HINT1 exhibits isopeptidase activity on sumoylated RanGAP1 in the presence of increasing concentrations of CaCl2 & of CaM. Details of immunoblot detection in “Materials & Methods” section & Supplementary Figures S5 & S6. GST, glutathione S-transferase; ICD, intracellular domain; RanGAP1, Ran GTPase-activating protein 1; RGSZ2, regulator of G protein signaling 17 (Z2); SENP, sentrin-specific protease. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31088288), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-41315_rabbit-polyclonal-teneurin-1-antibody-31020241611353.jpg)
![Immunocytochemistry/ Immunofluorescence: Teneurin-1 Antibody - BSA Free [NBP2-41315] - Immunofluorescence of Teneurin-1 in mouse brain tissue with Teneurin-1 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp2-41315_rabbit-polyclonal-teneurin-1-antibody-271120241932664.jpg)
![Immunohistochemistry: Teneurin-1 Antibody - BSA Free [NBP2-41315] - Immunohistochemistry of Teneurin-1 in mouse brain tissue with Teneurin-1 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41315_rabbit-polyclonal-teneurin-1-antibody-2711202419353882.jpg)
![Western Blot: GKAP/DLGAP1 Antibody [NBP1-76911] - Analysis in rat brain tissue lysate with antibody at (A) 0.5 and (B) 1 ug/mL.](http://images.novusbio.com/fullsize/GKAP-DLGAP1-Antibody-Western-Blot-NBP1-76911-img0004.jpg "Western Blot: GKAP/DLGAP1 Antibody [NBP1-76911] - Analysis in rat brain tissue lysate with antibody at (A) 0.5 and (B) 1 ug/mL.")
![Immunohistochemistry: GKAP/DLGAP1 Antibody - BSA Free [NBP1-76911] - Immunohistochemistry of GKAP/DLGAP1 in human brain with GKAP/DLGAP1 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76911_rabbit-polyclonal-gkap-dlgap1-antibody-271120241935388.jpg)
![Immunocytochemistry/ Immunofluorescence: GKAP/DLGAP1 Antibody - BSA Free [NBP1-76911] - Immunofluorescence of GKAP/DLGAP1 in Human Brain cells with GKAP/DLGAP1 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76911_rabbit-polyclonal-gkap-dlgap1-antibody-2711202419251521.jpg)
![Western Blot: ARF6 Antibody [NBP2-41263] - Analysis of ARF6 in rat liver tissue lysate with ARF6 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/ARF6-Antibody-Western-Blot-NBP2-41263-img0001.jpg "Western Blot: ARF6 Antibody [NBP2-41263] - Analysis of ARF6 in rat liver tissue lysate with ARF6 antibody at 1 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: ARF6 Antibody - BSA Free [NBP2-41263] - Immunofluorescence of ARF6 in human kidney tissue with ARF6 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41263_rabbit-polyclonal-arf6-antibody-2711202420324836.jpg)
![Immunohistochemistry: ARF6 Antibody - BSA Free [NBP2-41263] - Immunohistochemistry of ARF6 in human kidney tissue with ARF6 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41263_rabbit-polyclonal-arf6-antibody-2711202420212343.jpg)
![Western Blot: NLRC3 Antibody [NBP2-41123] - Analysis of NOD3 in 3T3 cell lysate with NOD3 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/NLRC3-Antibody-Western-Blot-NBP2-41123-img0001.jpg "Western Blot: NLRC3 Antibody [NBP2-41123] - Analysis of NOD3 in 3T3 cell lysate with NOD3 antibody at (A) 1 and (B) 2 ug/mL.")
![Immunocytochemistry/ Immunofluorescence: NLRC3 Antibody - BSA Free [NBP2-41123] - Immunofluorescence of NLRC3 in Jurkat cells with NLRC3 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41123_rabbit-polyclonal-nlrc3-antibody-2711202419172282.jpg)
![Western Blot: Parkin Antibody [NBP2-41287] - Analysis of PARK2 in human cerebellum tissue lysate with PARK2 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/Parkin-Antibody-Western-Blot-NBP2-41287-img0001.jpg "Western Blot: Parkin Antibody [NBP2-41287] - Analysis of PARK2 in human cerebellum tissue lysate with PARK2 antibody at 1 ug/ml.")
![Immunohistochemistry: Parkin Antibody - BSA Free [NBP2-41287] - Immunohistochemistry of Parkin in human brain tissue with Parkin antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41287_rabbit-polyclonal-parkin-antibody-271120241932611.jpg)
![Immunocytochemistry/ Immunofluorescence: Parkin Antibody - BSA Free [NBP2-41287] - Immunofluorescence of Parkin in human brain tissue with Parkin antibody at 20 u/ml.](http://images.novusbio.com/fullsize/nbp2-41287_rabbit-polyclonal-parkin-antibody-2711202420405583.jpg)
![Immunocytochemistry/ Immunofluorescence: Parkin Antibody - BSA Free [NBP2-41287] - Immunofluorescence of Parkin in human brain tissue with Parkin antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41287_rabbit-polyclonal-parkin-antibody-271120242074619.jpg)