Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76512-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77182-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76677SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76751-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77196-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41246-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77180-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76697-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77289-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76976-0.025mg | |||
![Western Blot: DPF2 Antibody [NBP1-76512] - Mouse kidney tissue lysate with DPF2 antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/DPF2-Antibody-Western-Blot-NBP1-76512-img0001.jpg "Western Blot: DPF2 Antibody [NBP1-76512] - Mouse kidney tissue lysate with DPF2 antibody at 1 ug/mL.")
![Immunohistochemistry-Paraffin: DPF2 Antibody [NBP1-76512] - Human kidney tissue with REQUIEM antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/DPF2-Antibody-Immunohistochemistry-Paraffin-NBP1-76512-img0004.jpg)
![Immunohistochemistry: DPF2 Antibody [NBP1-76512] - Human kidney tissue with DPF2 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/DPF2-Antibody-Immunofluorescence-NBP1-76512-img0003.jpg)
![Western Blot: TCTN1 Antibody [NBP1-77182] - Mouse kidney tissue lysate with TCTN1 antibody at (A) 1 and (B) 2ug/mL.](http://images.novusbio.com/fullsize/TCTN1-Antibody-Western-Blot-NBP1-77182-img0001.jpg "Western Blot: TCTN1 Antibody [NBP1-77182] - Mouse kidney tissue lysate with TCTN1 antibody at (A) 1 and (B) 2ug/mL.")
![Immunohistochemistry-Paraffin: TCTN1 Antibody [NBP1-77182] - Human kidney tissue with TCTN1 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/TCTN1-Antibody-Immunohistochemistry-Paraffin-NBP1-77182-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: TCTN1 Antibody - BSA Free [NBP1-77182] - Immunofluorescence of TCTN1 in Human Kidney cells with TCTN1 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77182_rabbit-polyclonal-tctn1-antibody-271120242013250.jpg)
![Immunohistochemistry: TCTN1 Antibody - BSA Free [NBP1-77182] - Immunohistochemistry of TCTN1 in human kidney tissue with TCTN1 antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp1-77182_rabbit-polyclonal-tctn1-antibody-2711202420414596.jpg)
![Immunocytochemistry/ Immunofluorescence: TCTN1 Antibody - BSA Free [NBP1-77182] - Immunofluorescence of TCTN1 in Human Kidney cells with TCTN1 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77182_rabbit-polyclonal-tctn1-antibody-2711202420324834.jpg)
![Immunohistochemistry: TCTN1 Antibody - BSA Free [NBP1-77182] - Immunohistochemistry of TCTN1 in human kidney tissue with TCTN1 antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp1-77182_rabbit-polyclonal-tctn1-antibody-2711202419241319.jpg)
![Western Blot: Nudel Antibody [NBP1-76677] - Jurkat whole cell lysate with Nudel antibody at (A) 0.5, (B) 1, or (C) 2 ug/ml.](http://images.novusbio.com/fullsize/Nudel-Antibody-Western-Blot-NBP1-76677-img0001.jpg "Western Blot: Nudel Antibody [NBP1-76677] - Jurkat whole cell lysate with Nudel antibody at (A) 0.5, (B) 1, or (C) 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: Nudel Antibody [NBP1-76677] - Immunofluorescence of Nudel in Jurkat cells with Nudel antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/Nudel-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76677-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Nudel Antibody [NBP1-76677] - Jurkat cells with Nudel antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Nudel-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76677-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Nudel Antibody - BSA Free [NBP1-76677] - Immunofluorescence of Nudel in Jurkat cells with Nudel antibody at 10 u/mL.](http://images.novusbio.com/fullsize/nbp1-76677_rabbit-polyclonal-nudel-antibody-2711202420195390.jpg)
![Immunocytochemistry/ Immunofluorescence: Nudel Antibody - BSA Free [NBP1-76677] - Immunocytochemistry of Nudel in Jurkat cells with Nudel antibody at 2 u/mL.](http://images.novusbio.com/fullsize/nbp1-76677_rabbit-polyclonal-nudel-antibody-271120241928851.jpg)
![Immunocytochemistry/ Immunofluorescence: Nudel Antibody - BSA Free [NBP1-76677] - Immunofluorescence of Nudel in Jurkat cells with Nudel antibody at 10 u/mL.](http://images.novusbio.com/fullsize/nbp1-76677_rabbit-polyclonal-nudel-antibody-2711202420392740.jpg)
![Immunocytochemistry/ Immunofluorescence: Nudel Antibody - BSA Free [NBP1-76677] - Immunocytochemistry of Nudel in Jurkat cells with Nudel antibody at 2 u/mL.](http://images.novusbio.com/fullsize/nbp1-76677_rabbit-polyclonal-nudel-antibody-2711202420324811.jpg)
![Western Blot: BANF1 Antibody [NBP1-76751] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: BANF1, NBP1-76751 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/BANF1-Antibody-Western-Blot-NBP1-76751-img0006.jpg "Western Blot: BANF1 Antibody [NBP1-76751] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: BANF1, NBP1-76751 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Western Blot: BANF1 Antibody [NBP1-76751] - Analysis in Hela cell lysate with antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/BANF1-Antibody-Western-Blot-NBP1-76751-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: BANF1 Antibody [NBP1-76751] - Independent Antibody Validation via Protein Expression in Human Lines. Loading: 15 ug of lysates per lane. Antibodies: BANF1 [NBP1-76750] (2 ug/ml), BANF1 [NBP1-76751] (2 ug/ml) and beta-actin (1.5 ug/ml). 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/BANF1-Antibody-Western-Blot-NBP1-76751-img0004.jpg)
![Western Blot: BANF1 Antibody [NBP1-76751] - Western Blot Validation in Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: BANF1, NBP1-76751 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/BANF1-Antibody-Western-Blot-NBP1-76751-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: BANF1 Antibody - BSA Free [NBP1-76751] - Figure 5 Immunofluorescence Validation of BANF1 in Human Kideny TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed human kidney tissue labeling BANF1 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-76751_rabbit-polyclonal-banf1-antibody-2711202420324830.jpg)
![Immunocytochemistry/Immunofluorescence: NAIP Antibody [NBP1-77196] - Confocal microscopy analysis of NAIP expression in THP-1m with or without CFT073 stimulation. NLRC4 and NAIP expression in THP-1m, 6 h post stimulation with or without (mock) CFT073 was examined with confocal microscopy analysis using anti-NAIP antibody. Alexa fluor 488 (green) was used to detect anti-NAIP antibody. Nucleus of cells were stained with DAPI (blue). Results are representative of three independent experiments. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fmicb.2019.02020/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NAIP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77196-img0009.jpg "<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NAIP Antibody [NBP1-77196] - Confocal microscopy analysis of NAIP expression in THP-1m with or without CFT073 stimulation. NLRC4 and NAIP expression in THP-1m, 6 h post stimulation with or without (mock) CFT073 was examined with confocal microscopy analysis using anti-NAIP antibody. Alexa fluor 488 (green) was used to detect anti-NAIP antibody. Nucleus of cells were stained with DAPI (blue). Results are representative of three independent experiments. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fmicb.2019.02020/full) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Western Blot: NAIP Antibody [NBP1-77196] - Analysis of NLRP3, NLRC4, NAIP, AIM2, ASC, Caspase-4, Caspase-1, B-actin and GAPDH protein expression by immunoblotting. (A) Represents the immunoblotting results of NLRP3, NLRC4, NAIP, Caspase-1, GAPDH of UPEC infected UTI patients and controls group. Scattered plots showing individual densitometric values (IDV) of NAIP (D). Results were expressed as average densitometric ratio in patients and controls group +/- SD. P-value is ***p = 0.0001 and n.s, non-significant. anti-NLRP3 antibody (NBP1-77080), anti-NLRC4 antibody (NBP2-41124), anti-Caspase-1 antibody (NBP1-45433). Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fmicb.2019.02020/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NAIP-Antibody-Western-Blot-NBP1-77196-img0008.jpg)
![Western Blot: NAIP Antibody - BSA Free [NBP1-77196] - Western blot analysis of NAIP in human tonsil tissue lysate with NAIP antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77196_rabbit-polyclonal-naip-antibody-2711202420405528.jpg)
![Immunocytochemistry/ Immunofluorescence: NAIP Antibody - BSA Free [NBP1-77196] - Immunofluorescence of NAIP in A549 cells with NAIP antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77196_rabbit-polyclonal-naip-antibody-271120242022931.jpg)
![Immunocytochemistry/ Immunofluorescence: NAIP Antibody - BSA Free [NBP1-77196] - Immunocytochemistry of NAIP in A549 cells with NAIP antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77196_rabbit-polyclonal-naip-antibody-2711202420352416.jpg)
![Western Blot: RPSA Antibody [NBP2-41246] - Analysis of RPSA in Caco-2 cell lysate with RPSA antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/RPSA-Antibody-Western-Blot-NBP2-41246-img0004.jpg "Western Blot: RPSA Antibody [NBP2-41246] - Analysis of RPSA in Caco-2 cell lysate with RPSA antibody at 1 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: RPSA Antibody - BSA Free [NBP2-41246] - Immunofluorescence of RPSA in mouse liver tissue with RPSA antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41246_rabbit-polyclonal-rpsa-antibody-2711202420195344.jpg)
![Immunohistochemistry: RPSA Antibody - BSA Free [NBP2-41246] - Immunohistochemistry of RPSA in mouse brain tissue with RPSA Antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41246_rabbit-polyclonal-rpsa-antibody-271120242045639.gif)
![Immunohistochemistry: RPSA Antibody - BSA Free [NBP2-41246] - Immunohistochemistry of RPSA in mouse liver tissue with RPSA antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp2-41246_rabbit-polyclonal-rpsa-antibody-271120242004162.jpg)
![Immunocytochemistry/ Immunofluorescence: RPSA Antibody - BSA Free [NBP2-41246] - Immunofluorescence of RPSA in mouse brain tissue with RPSA Antibody at 20 ug/mL.Green: RPSA antibody Red: Phylloidin staining Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp2-41246_rabbit-polyclonal-rpsa-antibody-271120242042517.gif)
![Western Blot: QBRICK/FREM1 Antibody [NBP1-77180] - K562 cell lysate with FREM1 antibody at (A) 0.5 and (B) 1ug/mL.](http://images.novusbio.com/fullsize/QBRICK-FREM1-Antibody-Western-Blot-NBP1-77180-img0001.jpg "Western Blot: QBRICK/FREM1 Antibody [NBP1-77180] - K562 cell lysate with FREM1 antibody at (A) 0.5 and (B) 1ug/mL.")
![Immunocytochemistry/Immunofluorescence: QBRICK/FREM1 Antibody [NBP1-77180] - K562 cells at 20 ug/mL.](http://images.novusbio.com/fullsize/QBRICK-FREM1-Antibody-Immunofluorescence-NBP1-77180-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: QBRICK/FREM1 Antibody [NBP1-77180] - K562 cells with FREM1 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/QBRICK-FREM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77180-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: QBRICK/FREM1 Antibody - BSA Free [NBP1-77180] - Immunofluorescence of QBRICK/FREM1 in K562 cells with QBRICK/FREM1 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77180_rabbit-polyclonal-qbrick-frem1-antibody-2711202419451244.jpg)
![Immunocytochemistry/ Immunofluorescence: QBRICK/FREM1 Antibody - BSA Free [NBP1-77180] - Immunofluorescence of QBRICK/FREM1 in K562 cells with QBRICK/FREM1 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77180_rabbit-polyclonal-qbrick-frem1-antibody-2711202419201522.jpg)
![Immunocytochemistry/ Immunofluorescence: QBRICK/FREM1 Antibody - BSA Free [NBP1-77180] - Immunocytochemistry of QBRICK/FREM1 in K562 cells with QBRICK/FREM1 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77180_rabbit-polyclonal-qbrick-frem1-antibody-2711202420352473.jpg)
![Immunocytochemistry/ Immunofluorescence: QBRICK/FREM1 Antibody - BSA Free [NBP1-77180] - Immunocytochemistry of QBRICK/FREM1 in K562 cells with QBRICK/FREM1 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77180_rabbit-polyclonal-qbrick-frem1-antibody-2711202420392768.jpg)
![Western Blot: IL-23A/IL-23 P19 Antibody [NBP1-76697] - Western Blot Validation in Mouse Tissue Lysate. Loading: 15 ug of lysates per lane. Antibodies: IL-23A/IL-23 P19 NBP1-76697 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/IL-23A-IL-23-P19-Antibody-Western-Blot-NBP1-76697-img0006.jpg "Western Blot: IL-23A/IL-23 P19 Antibody [NBP1-76697] - Western Blot Validation in Mouse Tissue Lysate. Loading: 15 ug of lysates per lane. Antibodies: IL-23A/IL-23 P19 NBP1-76697 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Immunohistochemistry-Paraffin: IL-23A/IL-23 P19 Antibody [NBP1-76697] - Mouse pancreas tissue with IL23 P19 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/IL-23A-IL-23-P19-Antibody-Immunohistochemistry-Paraffin-NBP1-76697-img0002.jpg)
![Western Blot: IL-23A/IL-23 P19 Antibody [NBP1-76697] - Mouse pancreas tissue lysate with IL-23 P19antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/IL-23A-IL-23-P19-Antibody-Western-Blot-NBP1-76697-img0003.jpg)
![<b>Expression of Tagged Proteins Validation. </b>Western Blot: IL-23A/IL-23 P19 Antibody [NBP1-76697] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human IL-23 recombinant protein per lane. Antibodies: IL-23A/IL-23 P19 NBP1-76697 (1: 0.25 ug/mL, 2: 0.5 ug/mL, 3: 1 ug/mL and 4: 2ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/IL-23A-IL-23-P19-Antibody-Western-Blot-NBP1-76697-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-76697] - Validation of IL-23A/IL-23 P19 in Mouse Pancreas Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse pancreas tissue labeling IL-23A/IL-23 P19 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-76697_rabbit-polyclonal-il-23a-il-23-p19-antibody-271120241954152.jpg)
![Western Blot: Orai1 Antibody - BSA Free [NBP1-77289] - Human ovary tissue lysate with ORAI1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.](http://images.novusbio.com/fullsize/Orai1-Antibody-Western-Blot-NBP1-77289-img0001.jpg "Western Blot: Orai1 Antibody - BSA Free [NBP1-77289] - Human ovary tissue lysate with ORAI1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.")
![Immunohistochemistry-Paraffin: Orai1 Antibody - BSA Free [NBP1-77289] - Analysis of ORAI1 in human ovary tissue with ORAI1 antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/Orai1-Antibody-Immunohistochemistry-NBP1-77289-img0003.jpg)
![Immunohistochemistry: Orai1 Antibody - BSA Free [NBP1-77289] - Immunohistochemistry of Orai1 in human spleen tissue with Orai1 antibody at 2.5 u/mL.](http://images.novusbio.com/fullsize/nbp1-77289_rabbit-polyclonal-orai1-antibody-271120242074628.jpg)
![Immunocytochemistry/ Immunofluorescence: Orai1 Antibody - BSA Free [NBP1-77289] - Immunofluorescence of Orai1 in human ovary tissue with Orai1 antibody at 20 ug/mL.Red: Orai1 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-77289_rabbit-polyclonal-orai1-antibody-2711202419241341.gif)
![Western Blot: alpha-Synuclein Antibody (5C2) [NBP1-04321] - The recombinant human alpha-synuclein domains were resolved by SDS-PAGE, transferred to PVDF membrane and probed with human anti-alpha-Synuclein(61-95aa) antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Recombinant human alpha-synuclein(1-60aa)Lane 2.: Recombinant human alpha-synuclein(1-95aa)Lane 3.: Recombinant human alpha-synuclein(61-140aa)Lane 4.: Recombinant human alpha-synuclein(96-140aa)](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-5C2-Western-Blot-NBP1-04321-img0002.jpg "Western Blot: alpha-Synuclein Antibody (5C2) [NBP1-04321] - The recombinant human alpha-synuclein domains were resolved by SDS-PAGE, transferred to PVDF membrane and probed with human anti-alpha-Synuclein(61-95aa) antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Recombinant human alpha-synuclein(1-60aa)Lane 2.: Recombinant human alpha-synuclein(1-95aa)Lane 3.: Recombinant human alpha-synuclein(61-140aa)Lane 4.: Recombinant human alpha-synuclein(96-140aa)")
![Western Blot: alpha-Synuclein Antibody (5C2) [NBP1-04321] - The recombinant human synuclein family proteins were resolved by SDS-PAGE, transferred to PVDF membrane and probed with human anti-alpha-Synuclein antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Recombinant human alpha-synucleinLane 2.: Recombinant human beta-synucleinLane 3.: Recombinant human gamma-synuclein](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-5C2-Western-Blot-NBP1-04321-img0001.jpg)
![Western Blot: EBI3 Antibody [NBP1-76976] - Analysis of EBI3 in human tonsil tissue lysate with EBI3 antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/EBI3-Antibody-Western-Blot-NBP1-76976-img0007.jpg "Western Blot: EBI3 Antibody [NBP1-76976] - Analysis of EBI3 in human tonsil tissue lysate with EBI3 antibody at 2 ug/mL.")
![Western Blot: EBI3 Antibody [NBP1-76976] - K562 lysate with EBI3 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/EBI3-Antibody-Western-Blot-NBP1-76976-img0005.jpg)
![Western Blot: EBI3 Antibody - BSA Free [NBP1-76976] - Recombinant Protein Validation. Loading: 30 ng of human recombinant IL-17 (IL-17A+IL-17B) protein per lane.Antibodies: EBI-3 , 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 1 ug/mL, Lane 2: 2 ug/mL, Lane 3: 4 ug/mL,](http://images.novusbio.com/fullsize/nbp1-76976_rabbit-polyclonal-ebi3-antibody-271120242016957.gif)
![Immunohistochemistry: EBI3 Antibody - BSA Free [NBP1-76976] - Validation of EBI-3 in Human Spleen Tissue. Immunohistochemical analysis of paraffin-embedded Human Spleen tissue using anti-EBI-3 antibody at 2.5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76976_rabbit-polyclonal-ebi3-antibody-2711202419475868.jpg)
![Immunocytochemistry/ Immunofluorescence: EBI3 Antibody - BSA Free [NBP1-76976] - Validation of EBI-3 in Human Spleen Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Spleen tissue labeling EBI-3 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-76976_rabbit-polyclonal-ebi3-antibody-2711202420105819.jpg)
![Western Blot: PCK2 Antibody [NB100-2422] - Staining of NIH3T3 cell lysate with antibody at 2 ug/mL (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PCK2-Antibody-Western-Blot-NB100-2422-img0002.jpg "Western Blot: PCK2 Antibody [NB100-2422] - Staining of NIH3T3 cell lysate with antibody at 2 ug/mL (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: PCK2 Antibody [NB100-2422] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2422-img0003.jpg)
![Flow Cytometry: PCK2 Antibody [NB100-2422] - Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/PCK2-Antibody-Flow-Cytometry-NB100-2422-img0004.jpg)
![Western Blot: PCK2 Antibody [NB100-2422] - Staining of A431 (A), HEK293 (B) with antibody at 2 ug/mL and HepG2 (C) cell lysate with antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PCK2-Antibody-Western-Blot-NB100-2422-img0001.jpg)