Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | NB7308APC | |||
| Biotin | NB7308B | |||
| DyLight 350 | NB7308UV | |||
| DyLight 405 | NB7308V | |||
| DyLight 488 | NB7308G | |||
| DyLight 550 | NB7308R | |||
| DyLight 594 | NB7308DL594 | |||
| DyLight 650 | NB7308C | |||
| DyLight 680 | NB7308FR | |||
| DyLight 755 | NB7308IR | |||
| FITC | NB7310 | |||
| HRP | NB7311 | |||
| Janelia Fluor 525 | NB7308JF525 | |||
| Janelia Fluor 669 | NB7308JF669 | |||
| PE | NB7308PE | |||
| PerCP | NB7308PCP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54473-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | FAB1642P | |||
| Carrier Free | MAB1642-SP | |||
| Carrier Free | FAB1642V-100UG | |||
| Carrier Free | FAB1642U-100UG | |||
| Carrier Free | FAB1642T-100UG | |||
| Carrier Free | FAB1642S-100UG | |||
| Carrier Free | FAB1642R-100UG | |||
| Carrier Free | FAB1642N-100UG | |||
| Carrier Free | FAB1642G-100UG | |||
![N/A Angiopoietin-like Protein 3/ANGPTL3 [HRP]](http://images.novusbio.com/fullsize2/DATA_Angiopoietinlike_Protein_3_MANL30_ELISA_940.jpg "N/A Angiopoietin-like Protein 3/ANGPTL3 [HRP]")
![N/A Angiopoietin-like Protein 3/ANGPTL3 [HRP]](http://images.novusbio.com/fullsize2/Angiopoietin-like_Protein_3_MANL30_ELISA_239.jpg)
![N/A Corin [HRP]](http://images.novusbio.com/fullsize2/DATA_Corin_DCRN00_ELISA_635.jpg "N/A Corin [HRP]")
![N/A Corin [HRP]](http://images.novusbio.com/fullsize2/Corin_DCRN00_ELISA_68.jpg)
![N/A CEACAM1/CD66a [Biotin]](http://images.novusbio.com/fullsize2/DATA_CEACAM1_DY2244_ELISA_1793.jpg "N/A CEACAM1/CD66a [Biotin]")
![Flow Cytometry: Sheep Polyclonal Fetal Hemoglobin Antibody [NB110-41084] - HbF cells in mouse. These mice are under stressed erythropoiesis. This antibody used to detect fetal hemoglobin in RBC. RBC was fixed by paraformaldehyde followed by permeabilization with 0.01% triton. These cells were incubated with antibody at 1:500 dilution for 30-60 min at room temperature. Image from a verified customer review. The customer used the Alexa Fluor® 647-conjugated version of NB110-41084, which is listed under NB110-41084AF647.](http://images.novusbio.com/fullsize/antibody/nb110-41084_sheep-polyclonal-fetal-hemoglobin-antibody-flow-cytometry-295202514214..jpg "Flow Cytometry: Sheep Polyclonal Fetal Hemoglobin Antibody [NB110-41084] - HbF cells in mouse. These mice are under stressed erythropoiesis. This antibody used to detect fetal hemoglobin in RBC. RBC was fixed by paraformaldehyde followed by permeabilization with 0.01% triton. These cells were incubated with antibody at 1:500 dilution for 30-60 min at room temperature. Image from a verified customer review. The customer used the Alexa Fluor® 647-conjugated version of NB110-41084, which is listed under NB110-41084AF647.")
![Western Blot: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239] - Western Blot Analysis of Raji cell lysate using Siglec-2/CD22 Antibody (BLCAM/1795).](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-BLCAM-1795-Western-Blot-NBP2-53239-img0008.jpg "Western Blot: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239] - Western Blot Analysis of Raji cell lysate using Siglec-2/CD22 Antibody (BLCAM/1795).")
![Immunocytochemistry/Immunofluorescence: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239] - Immunofluorescent staining of paraformaldehyde-fixed Ramos cells using Siglec-2/CD22 Antibody (BLCAM/1795) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-BLCAM-1795-Immunocytochemistry-Immunofluorescence-NBP2-53239-img0005.jpg)
![Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239] - Formalin-fixed, paraffin-embedded human Spleen stained with Siglec-2/CD22 Antibody (BLCAM/1795).](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-BLCAM-1795-Immunohistochemistry-Paraffin-NBP2-53239-img0006.jpg)
![Flow Cytometry: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239] - Flow Cytometric Analysis of paraformaldehyde-fixed MOLT4 cells. Siglec-2/CD22 Antibody (BLCAM/1795) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-BLCAM-1795-Flow-Cytometry-NBP2-53239-img0004.jpg)
![Western Blot: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239] - Analysis of Raji and Ramos Cell Lysates using CD22 Monoclonal Antibody (BLCAM/1795).](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-BLCAM-1795-Western-Blot-NBP2-53239-img0001.jpg)
![Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD22 Monoclonal Antibody (BLCAM/1795).](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-BLCAM-1795-Immunohistochemistry-Paraffin-NBP2-53239-img0002.jpg)
![Protein Array: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Siglec-2/CD22 Antibody (BLCAM/1795) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-BLCAM-1795-Protein-Array-NBP2-53239-img0007.jpg)
![N/A IL-36 gamma/IL-1F9 [Biotin]](http://images.novusbio.com/fullsize2/DATA_IL36_gamma_DY232005_ELISA_2390.jpg "N/A IL-36 gamma/IL-1F9 [Biotin]")
![N/A EGFR [HRP]](http://images.novusbio.com/fullsize2/DATA_EGF_R_DEGFR0_ELISA_655.jpg "N/A EGFR [HRP]")
![N/A EGFR [HRP]](http://images.novusbio.com/fullsize2/DATA_EGF_R_DEGFR0_ELISA_656.jpg)
![N/A EGFR [HRP]](http://images.novusbio.com/fullsize2/EGF_R_DEGFR0_ELISA_80.jpg)
![N/A C-Peptide [HRP]](http://images.novusbio.com/fullsize2/DATA_Insulin_DICP00_ELISA_720.jpg "N/A C-Peptide [HRP]")
![N/A C-Peptide [HRP]](http://images.novusbio.com/fullsize2/Insulin_DICP00_ELISA_478.jpg)