Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | FAB1642P | |||
| Carrier Free | MAB1642-SP | |||
| Carrier Free | FAB1642V-100UG | |||
| Carrier Free | FAB1642U-100UG | |||
| Carrier Free | FAB1642T-100UG | |||
| Carrier Free | FAB1642S-100UG | |||
| Carrier Free | FAB1642R-100UG | |||
| Carrier Free | FAB1642N-100UG | |||
| Carrier Free | FAB1642G-100UG | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | NB7222APC | |||
| Biotin | NB7222B | |||
| DyLight 350 | NB7222UV | |||
| DyLight 405 | NB7222V | |||
| DyLight 488 | NB7222G | |||
| DyLight 550 | NB7222R | |||
| DyLight 594 | NB7222DL594 | |||
| DyLight 650 | NB7222C | |||
| DyLight 680 | NB7222FR | |||
| DyLight 755 | NB7222IR | |||
| FITC | NB7223 | |||
| HRP | NB7224 | |||
| PE | NB7222PE | |||
| PerCP | NB7222PCP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1920-SP | |||
![N/A IL-36 beta/IL-1F8 [Biotin]](http://images.novusbio.com/fullsize2/DATA_IL36_beta_DY109905_ELISA_2340.jpg "N/A IL-36 beta/IL-1F8 [Biotin]")
![Immunocytochemistry/Immunofluorescence: Monoamine Oxidase B Antibody [NBP1-32426] - HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: Monoamine Oxidase B stained by Monoamine Oxidase B antibody [N2C3] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32426-img0011.jpg "Immunocytochemistry/Immunofluorescence: Monoamine Oxidase B Antibody [NBP1-32426] - HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: Monoamine Oxidase B stained by Monoamine Oxidase B antibody [N2C3] diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody [NBP1-32426] - Mouse liver. Monoamine Oxidase B stained by Monoamine Oxidase B antibody [N2C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-Immunohistochemistry-Paraffin-NBP1-32426-img0010.jpg)
![Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody [NBP1-32426] - Mouse lung. Monoamine Oxidase B antibody [N2C3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-Immunohistochemistry-Paraffin-NBP1-32426-img0007.jpg)
![ELISA: Monoamine Oxidase B Antibody [NBP1-32426] - ELISA detection of MAOB using Monoamine Oxidase B antibody [N2C3] for capture at a concentration of 2.5 ug/mL and monoamine oxidase B for detection at a concentration of 0.5 ug/mL.](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-ELISA-NBP1-32426-img0006.jpg)
![Western Blot: Monoamine Oxidase B Antibody [NBP1-32426] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Monoamine Oxidase B antibody [N2C3] (NBP1-32426) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32426_rabbit-polyclonal-monoamine-oxidase-b-antibody-910202420134011.jpg)
![<b>Orthogonal Strategies Validation. </b>HepG2 whole cell and mitochondria extracts (8.1 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Monoamine Oxidase B antibody [N2C3] (NBP1-32426) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32426_rabbit-polyclonal-monoamine-oxidase-b-antibody-western-blot-141120251843448.jpg)
![HepG2 whole cell and mitochondria extracts (8.1 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Monoamine Oxidase B antibody [N2C3] (NBP1-32426) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32426_rabbit-polyclonal-monoamine-oxidase-b-antibody-western-blot-261120251854115.jpg)
![N/A Angiopoietin-like Protein 3/ANGPTL3 [HRP]](http://images.novusbio.com/fullsize2/DATA_Angiopoietinlike_Protein_3_MANL30_ELISA_940.jpg "N/A Angiopoietin-like Protein 3/ANGPTL3 [HRP]")
![N/A Angiopoietin-like Protein 3/ANGPTL3 [HRP]](http://images.novusbio.com/fullsize2/Angiopoietin-like_Protein_3_MANL30_ELISA_239.jpg)
![N/A IL-28B/IFN-lambda 3 [HRP]](http://images.novusbio.com/fullsize2/IL28B_D28B00_ELISA_1128.jpg "N/A IL-28B/IFN-lambda 3 [HRP]")
![N/A IL-28B/IFN-lambda 3 [HRP]](http://images.novusbio.com/fullsize2/IL28B_D28B00_ELISA_1127.jpg)
![N/A CEACAM1/CD66a [Biotin]](http://images.novusbio.com/fullsize2/DATA_CEACAM1_DY2244_ELISA_1793.jpg "N/A CEACAM1/CD66a [Biotin]")
![N/A Corin [HRP]](http://images.novusbio.com/fullsize2/DATA_Corin_DCRN00_ELISA_635.jpg "N/A Corin [HRP]")
![N/A Corin [HRP]](http://images.novusbio.com/fullsize2/Corin_DCRN00_ELISA_68.jpg)
