Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61784-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37300-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05120-0.025mg | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | DYC1387B-5 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | DYC1387B-5 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB95471-SP | |||
| Carrier Free | FAB95471R-100UG | |||
| Carrier Free | FAB95471N-100UG | |||
| Carrier Free | FAB95471G-100UG | |||
| Carrier Free | FAB95471V-100UG | |||
| Carrier Free | FAB95471U-100UG | |||
| Carrier Free | FAB95471T-100UG | |||
| Carrier Free | FAB95471S-100UG | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | NB7122APC | |||
| Biotin | NB7122B | |||
| DyLight 350 | NB7122UV | |||
| DyLight 405 | NB7122V | |||
| DyLight 488 | NB7122G | |||
| DyLight 550 | NB7122R | |||
| DyLight 594 | NB7122DL594 | |||
| DyLight 680 | NB7122FR | |||
| DyLight 755 | NB7122IR | |||
| FITC | NB7124 | |||
| HRP | NB7126 | |||
| Janelia Fluor 525 | NB7122JF525 | |||
| Janelia Fluor 669 | NB7122JF669 | |||
| PE | NB7122PE | |||
| PerCP | NB7122PCP | |||
![Western Blot: Phospholipase D2 Antibody (7E4D9) [NBP2-61784] - Analysis using PLD2 mAb against human PLD2 (AA: 834-933) recombinant protein. (Expected MW is 37.4 kDa)](http://images.novusbio.com/fullsize/Phospholipase-D2-Antibody-7E4D9-Western-Blot-NBP2-61784-img0007.jpg "Western Blot: Phospholipase D2 Antibody (7E4D9) [NBP2-61784] - Analysis using PLD2 mAb against human PLD2 (AA: 834-933) recombinant protein. (Expected MW is 37.4 kDa)")
![Immunocytochemistry/Immunofluorescence: Phospholipase D2 Antibody (7E4D9) [NBP2-61784] - Analysis of SK-OV-3 cells using PLD2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Phospholipase-D2-Antibody-7E4D9-Immunocytochemistry-Immunofluorescence-NBP2-61784-img0004.jpg)
![Immunohistochemistry: Phospholipase D2 Antibody (7E4D9) [NBP2-61784] - Analysis of paraffin-embedded esophageal cancer tissues using PLD2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Phospholipase-D2-Antibody-7E4D9-Immunohistochemistry-NBP2-61784-img0006.jpg)
![Flow Cytometry: Phospholipase D2 Antibody (7E4D9) [NBP2-61784] - Analysis of Hela cells using PLD2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Phospholipase-D2-Antibody-7E4D9-ELISA-NBP2-61784-img0002.jpg)
![Western Blot: Phospholipase D2 Antibody (7E4D9) [NBP2-61784] - Analysis using PLD2 mAb against HEK293 (1) and PLD2 (AA: 834-933)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Phospholipase-D2-Antibody-7E4D9-Western-Blot-NBP2-61784-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Phospholipase D2 Antibody (7E4D9) [NBP2-61784] - Analysis of MCF-7 cells using PLD2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Phospholipase-D2-Antibody-7E4D9-Immunocytochemistry-Immunofluorescence-NBP2-61784-img0003.jpg)
![Immunohistochemistry: Phospholipase D2 Antibody (7E4D9) [NBP2-61784] - Analysis of paraffin-embedded renal tissues using PLD2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Phospholipase-D2-Antibody-7E4D9-Immunohistochemistry-NBP2-61784-img0005.jpg)
![ELISA: Phospholipase D2 Antibody (7E4D9) [NBP2-61784] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Phospholipase-D2-Antibody-7E4D9-ELISA-NBP2-61784-img0001.jpg)
![Western Blot: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis using PLIN2 mouse mAb against HepG2 cell lysate.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Western-Blot-NBP2-37300-img0004.jpg "Western Blot: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis using PLIN2 mouse mAb against HepG2 cell lysate.")
![Immunocytochemistry/Immunofluorescence: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Immunofluorescence analysis of HepG2 cells using PLIN2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Immunocytochemistry-Immunofluorescence-NBP2-37300-img0005.jpg)
![Immunohistochemistry-Paraffin: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis of bladder cancer tissues using PLIN2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Immunohistochemistry-NBP2-37300-img0008.jpg)
![Flow Cytometry: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis of HepG2 cells using PLIN2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Flow-Cytometry-NBP2-37300-img0006.jpg)
![Western Blot: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis using PLIN2 mAb against human PLIN2 (AA: 286-437) recombinant protein. (Expected MW is 42.6 kDa)](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Western-Blot-NBP2-37300-img0002.jpg)
![Western Blot: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis using PLIN2 mAb against HEK293 (1) and PLIN2 (AA: 286-437)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Western-Blot-NBP2-37300-img0003.jpg)
![Immunohistochemistry-Paraffin: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis of rectum cancer tissues using PLIN2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-Immunohistochemistry-NBP2-37300-img0007.jpg)
![ELISA: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-2C5H8-ELISA-NBP2-37300-img0001.jpg)
![Immunohistochemistry-Frozen: C5b-9 Antibody (aE11) - BSA Free [NBP1-05120] - Vascular endothelia and dendritic cells of germinal center have strong and intensive label. Dilution: 1:10.](http://images.novusbio.com/fullsize/C5b-9-Antibody-aE11-BSA-Free-Immunohistochemistry-Frozen-NBP1-05120-img0002.jpg "Immunohistochemistry-Frozen: C5b-9 Antibody (aE11) - BSA Free [NBP1-05120] - Vascular endothelia and dendritic cells of germinal center have strong and intensive label. Dilution: 1:10.")
![Immunocytochemistry/Immunofluorescence: VE Cadherin Antibody (BV9) [NB600-1409] - Endothelial cells were fixed with 4% PAF (15 min, room temp), and then permeabilized with 0.5% TritionX-100 (3 min, room temperature). Cells were incubated with a final concentration of 10mg/ml BV9. Secondary detection was performed with anti-mouse Alexa-Fluor 647 and counterstained using DAPI.](http://images.novusbio.com/fullsize/VE-Cadherin-Antibody-BV9-Immunocytochemistry-Immunofluorescence-NB600-1409-img0008.jpg "Immunocytochemistry/Immunofluorescence: VE Cadherin Antibody (BV9) [NB600-1409] - Endothelial cells were fixed with 4% PAF (15 min, room temp), and then permeabilized with 0.5% TritionX-100 (3 min, room temperature). Cells were incubated with a final concentration of 10mg/ml BV9. Secondary detection was performed with anti-mouse Alexa-Fluor 647 and counterstained using DAPI.")
![Flow Cytometry: VE Cadherin Antibody (BV9) [NB600-1409] - HUVEC cells were stained with antibody BV9 in PBS/0.1% saponin for 1h at 4 degrees C.(Black- no stain, Red- isotype control, Blue-HM2032-BV9)](http://images.novusbio.com/fullsize/VE-Cadherin-Antibody-BV9-Flow-Cytometry-NB600-1409-img0007.jpg)
![Western Blot: TGF-beta 1 Antibody [NBP1-03276] - Each lane contains 250 ng of protein under non-reducing (lane 1) and reducing conditions (lane 2). The blot was incubated with a 1:200 dilution of the antibody at room temperature for 1 h followed by detection using labeled Goat-a-Rabbit IgG (H&L).](http://images.novusbio.com/fullsize/TGF-beta-1-Antibody-Western-Blot-NBP1-03276-img0002.jpg "Western Blot: TGF-beta 1 Antibody [NBP1-03276] - Each lane contains 250 ng of protein under non-reducing (lane 1) and reducing conditions (lane 2). The blot was incubated with a 1:200 dilution of the antibody at room temperature for 1 h followed by detection using labeled Goat-a-Rabbit IgG (H&L).")
![Immunohistochemistry-Paraffin: TGF-beta 1 Antibody [NBP1-03276] - Staining of human spleen tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize/TGF-beta-1-Antibody-Immunohistochemistry-Paraffin-NBP1-03276-img0003.jpg)
![Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01] - Analysis of EEF1G expression in transfected 293T cell line by EEF1G monoclonal antibody (M01), clone 3F11-1A10.Lane 1: EEF1G transfected lysate(50 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/EEF1G-Antibody-3F11-1A10-Western-Blot-H00001937-M01-img0016.jpg "Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01] - Analysis of EEF1G expression in transfected 293T cell line by EEF1G monoclonal antibody (M01), clone 3F11-1A10.Lane 1: EEF1G transfected lysate(50 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: EEF1G Antibody (3F11-1A10) [H00001937-M01] - Analysis of monoclonal antibody to EEF1G on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/EEF1G-Antibody-3F11-1A10-Immunocytochemistry-Immunofluorescence-H00001937-M01-img0008.jpg)
![Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01] - Analysis of EEF1G over-expressed 293 cell line, cotransfected with EEF1G Validated Chimera RNAi ( Cat # H00001937-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with EEF1G monoclonal antibody (M01), clone 3F11-1A10 (Cat # H00001937-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/EEF1G-Antibody-3F11-1A10-Western-Blot-H00001937-M01-img0009.jpg)
![Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01] - EEF1G monoclonal antibody (M01), clone 3F11-1A10. Analysis of EEF1G expression in PC-12.](http://images.novusbio.com/fullsize/EEF1G-Antibody-3F11-1A10-Western-Blot-H00001937-M01-img0010.jpg)
![Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01] - EEF1G monoclonal antibody (M01), clone 3F11-1A10. Analysis of EEF1G expression in HepG2.](http://images.novusbio.com/fullsize/EEF1G-Antibody-3F11-1A10-Western-Blot-H00001937-M01-img0011.jpg)
![Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01] - EEF1G monoclonal antibody (M01), clone 3F11-1A10. Analysis of EEF1G expression in 293.](http://images.novusbio.com/fullsize/EEF1G-Antibody-3F11-1A10-Western-Blot-H00001937-M01-img0012.jpg)
![Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01] - EEF1G monoclonal antibody (M01), clone 3F11-1A10 Analysis of EEF1G expression in HL-60.](http://images.novusbio.com/fullsize/EEF1G-Antibody-3F11-1A10-Western-Blot-H00001937-M01-img0013.jpg)
![Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01] - EEF1G monoclonal antibody (M01), clone 3F11-1A10. Analysis of EEF1G expression in Raw 264.7.](http://images.novusbio.com/fullsize/EEF1G-Antibody-3F11-1A10-Western-Blot-H00001937-M01-img0014.jpg)
![Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01] - EEF1G monoclonal antibody (M01), clone 3F11-1A10. Analysis of EEF1G expression in NIH/3T3.](http://images.novusbio.com/fullsize/EEF1G-Antibody-3F11-1A10-Western-Blot-H00001937-M01-img0015.jpg)
![ELISA JNK1/2/3 [Biotin]](http://images.novusbio.com/fullsize2/dyc1387b-2_phospho-jnk-pan-specific-duoset-ic-elisa-2-plate-elisa-169202095629.jpg "ELISA JNK1/2/3 [Biotin]")
![N/A ACE/CD143 [HRP]](http://images.novusbio.com/fullsize2/DATA_ACE_DACE00_ELISA_562.jpg "N/A ACE/CD143 [HRP]")
![N/A ACE/CD143 [HRP]](http://images.novusbio.com/fullsize2/DATA_ACE_DACE00_ELISA_563.jpg)
![N/A ACE/CD143 [HRP]](http://images.novusbio.com/fullsize2/ACE_DACE00_ELISA_22.jpg)
![N/A Periostin/OSF-2 [HRP]](http://images.novusbio.com/fullsize2/DATA_Periostin_MOSF20_ELISA_1012.jpg "N/A Periostin/OSF-2 [HRP]")
![N/A Periostin/OSF-2 [HRP]](http://images.novusbio.com/fullsize2/Periostin_MOSF20_ELISA_303.jpg)
![N/A MMP-9/TIMP-1 Complex [Biotin]](http://images.novusbio.com/fullsize2/DATA_MMP9_TIMP1_Complex_DY1449_ELISA_1649.jpg "N/A MMP-9/TIMP-1 Complex [Biotin]")