Goat anti-Rat IgG2a Heavy Chain Secondary Antibody

• Reactivity: Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Goat anti-Rat IgG2a Heavy Chain Secondary Antibody Summary

• Immunogen: Rat IgG2a
• Specificity: By immunoelectrophoresis and ELISA this reacts specifically with Rat IgG2a. This may cross react with IgG2a from other species.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000-1:30000 (Primary) 1:100-1:500 (for coating plates)
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:2000
  • Immunohistochemistry 1:200 - 1:2000
  • Immunohistochemistry-Paraffin 1:200 - 1:2000
  • Western Blot 1:1000-1:30000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate Buffered Saline (PBS)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG2a Antibody (NB7122)(10)

Publications using NB7122

• Mallén A, Rodriguez-Urquia R, Alvarez R et al. Sex Differences In Glomerular Lesions, Atherosclerosis Progression, And In The Response To Angiotensin-Converting Enzyme Inhibitors In The Apoe-/- Mice Model Preprints.org 2023-06-14 [PMID: 37686247]
• You Y, Muraoka S, Jedrychowski MP et al. Human neural cell type-specific�extracellular vesicle proteome defines disease-related molecules associated with activated astrocytes in Alzheimer's disease brain Journal of Extracellular Vesicles 2022-01-13 [PMID: 35029059]
• Gagan JM, Cao K, Zhang YA Et al. Constitutive transgenic a-Klotho overexpression enhances resilience to and recovery from murine acute lung injury Am J Physiol Lung Cell Mol Physiol 2021-08-04 [PMID: 34346778] (WB)
  
  Details:
  Citation using the HRP version of this antibody.
• Kohlmorgen C, Gerfer S, Feldmann K et al. Dapagliflozin reduces thrombin generation and platelet activation: implications for cardiovascular risk reduction in type 2 diabetes mellitus Diabetologia 2021-06-16 [PMID: 34131781]
  
  Details:
  This citation used the HRP format of this antibody.
• Jin H, Wu Y, Bi S et al. Higher immune response induced by vaccination in Houhai acupoint relates to the lymphatic drainage of the injection site Res Vet Sci 2020-04-01 [PMID: 32224352] (ELISA)
• Grandoch M, Fl�gel U, Virtue S et al. 4-Methylumbelliferone improves the thermogenic capacity of brown adipose tissue Nature Metabolism 2019-10-12 [PMID: 31602424]
• Barth M, Selig JI, Klose S et al. Degenerative aortic valve disease and diabetes: Implications for a link between proteoglycans and diabetic disorders in the aortic valve Diab Vasc Dis Res 2018-12-20 [PMID: 30563371] (IF/IHC)
• Schurich A, Khanna P, Lopes AR et al. Role of the coinhibitory receptor cytotoxic T lymphocyte antigen-4 on apoptosis-Prone CD8 T cells in persistent hepatitis B virus infection. Hepatology. [PMID: 21360567]
  
  Details:
  Citation using the FITC form of this antibody.
• Lin Y, Liang Z, Chen Y et al. TLR3-involved modulation of pregnancy tolerance in double-stranded RNA-stimulated NOD/SCID mice. J Immunol. [PMID: 16547251]
  
  Details:
  Citation using the FITC form of this antibody.
• Schmidt W, Reymann KG. Proliferating cells differentiate into neurons in the hippocampal CA1 region of gerbils after global cerebral ischemia. Neurosci Lett. [PMID: 12453618]
  
  Details:
  Citation using the FITC form of this antibody.