C5b-9 Antibody (aE11) [Alexa Fluor® 488]

• Reactivity: Hu, Po, Ba, Eq, Pm
• Applications: ELISA, Flow, ICC/IF, IHC, WB (-)
• Clone: aE11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

C5b-9 Antibody (aE11) [Alexa Fluor® 488] Summary

• Immunogen: Purified C5b-9
• Epitope: Binds to a neoepitope exposed on C9.
• Specificity: Binds both membrane-bound MAC (active) and fluid-phase SC5b-9 complexes (inactive).
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Use in Bacteria reported in scientific literature (PMID:32900809).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for C5b-9 Antibody (aE11) [Alexa Fluor® 488]

• MAC
• Membrane attack complex
• sC5-b9
• sC5b-9
• TCC
• Terminal complement complex

Background

C5b-9 is also known as the terminal complement complex (TCC). The TCC consists of C5b, C6, C7, C8 and C9 and forms the membrane attack complex (MAC) as well as the non-lytic fluid-phase SC5b-9 complex (with protein S). The MAC forms channels in target cell membranes leading to cell lysis by osmotic leakage. The complexes contain neoantigens that are absent from the individual native components from which they are formed and this antibody is directed against a neoepitope exposed on C9 when incorporated into the TCC (1,2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.