Trastuzumab Antibody (2261E) [Alexa Fluor® 647]

MCF-7 Human cell line was stained with Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Antibody (Catalog # MAB9589), then Anti- Trastuzumab (Anti-Idiotype) Alexa Fluor® 647-conjugated Antibody (Catalog # FAB95471R, filled histogram) or Rabbit IgG Isotype Control Antibody (Catalog # IC1051R, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: All-Multi
• Applications: Flow
• Clone: 2261E
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Alexa Fluor 647

Trastuzumab Antibody (2261E) [Alexa Fluor® 647] Summary

• Additional Information: Recombinant Monoclonal Antibodies
• Immunogen: Full-length Trastuzumab
• Specificity: Detects Trastuzumab in Western blots and direct ELISAs. In direct ELISAs, no cross-reactivity with Cetuximab, Ibritumomab, Rituximab, Adalimumab, Tositumomab, Brentuximab, Gemtuzumab, Solanezumab, Teprotumumab, and Aducanumab is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25-1 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Trastuzumab Antibody (2261E) [Alexa Fluor® 647]

• Trastuzumab

Background

The anti-Trastuzumab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Trastuzumab. The antibody has been tested against a broad range of other biosimilars to ensure specificity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Trastuzumab Antibody (FAB95471R)(1)

Publication using FAB95471R

• Z Yang, L Li, A Turkoz, P Chen, R Harari-Ste, M Bobbin, O Stefanson, H Choi, V Pietrobon, B Alphson, A Goswami, V Balan, A Kearney, D Patel, J Yang, D Inel, V Vinod, A Cesano, B Wang, KH Roh, LS Qi, FM Marincola Contextual reprogramming of CAR-T cells for treatment of HER2+ cancers Journal of Translational Medicine, 2021-11-07;19(1):459. 2021-11-07 [PMID: 34743703] (Flow Cytometry, Human)