Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-26297SS | |||
| Azide and BSA Free | NBP2-80867 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | FAB9727S-100UG | |||
| Carrier Free | FAB9727T-100UG | |||
| Carrier Free | FAB9727G-100UG | |||
| Carrier Free | FAB9727N-100UG | |||
| Carrier Free | FAB9727V-100UG | |||
| Carrier Free | FAB9727R-100UG | |||
| Carrier Free | FAB9727U-100UG | |||
| Carrier Free | MAB9727-SP | |||
![N/A CXCL16 [Biotin]](http://images.novusbio.com/fullsize2/DATA_CXCL16_DY1164_ELISA_1590.jpg "N/A CXCL16 [Biotin]")
![Western Blot: MRCL3 [p Ser19, p Ser20] Antibody [NB100-1687] - This antibody has been shown to cross-react with mouse skeletal muscle regulatory light chain (Lai et al., 2016. Am J Physiol Endocrinol Metab), and is shown to positively react with mouse EDL muscle here. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/MRCL3-[p-Ser19--p-Ser20]-Antibody-Western-Blot-NB100-1687-img0019.jpg "Western Blot: MRCL3 [p Ser19, p Ser20] Antibody [NB100-1687] - This antibody has been shown to cross-react with mouse skeletal muscle regulatory light chain (Lai et al., 2016. Am J Physiol Endocrinol Metab), and is shown to positively react with mouse EDL muscle here. This image was submitted via customer Review.")
![Immunohistochemistry: MRCL3 [p Ser19, p Ser20] Antibody [NB100-1687] - Used at 2.5 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows strong staining of both vascular and myometrial smooth muscle cells of the uterus. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/MRCL3-[p-Ser19--p-Ser20]-Antibody-Immunohistochemistry-NB100-1687-img0015.jpg)
![Western Blot: MRCL3 [p Ser19, p Ser20] Antibody [NB100-1687] - Affinity purified phosphospecific antibody to monophosphorylated regulatory light chain of smooth and non-muscle Myosin at pS19 was used at a 1:1000 dilution to detect myosin light chain by Western blot. Approximately 12 ul of a mouse cardiac myocyte lysate was loaded on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated or insulin or CLA treated according as indicated. After washing, a 1:5,000 dilution of HRP conjugated Gta- Rabbit IgG (611-103-122) preceded color development using Amersham's substrate system. Other detection methods will yield similar results.](http://images.novusbio.com/fullsize/MRCL3-[p-Ser19--p-Ser20]-Antibody-Western-Blot-NB100-1687-img0014.jpg)
![Western Blot: MRCL3 [p Ser19, p Ser20] Antibody [NB100-1687] - Used at a 1:5000 dilution to detect myosin light chain by Western blot. Either 13 or 20 l of a mouse cardiac myocyte lysate was loaded on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock-treated or CLA-treated, as indicated. After washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development using Amersham's substrate system.](http://images.novusbio.com/fullsize/MRCL3-[p-Ser19--p-Ser20]-Antibody-Western-Blot-NB100-1687-img0016.jpg)
![Western Blot: MRCL3 [p Ser19, p Ser20] Antibody [NB100-1687] - Lane 1: Regulatory Light Chain Non-Phospho recombinant protein. Lane 2: Regulatory Light Chain Phospho recombinant protein. Lane 3: Smooth Muscle Non-Phospho recombinant protein. Lane 4: Smooth Muscle Phospho recombinant protein. Load: 50 ng per lane. Primary antibody: Myosin pS19/pS20 primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 20 kDa, 20 kDa for Regulatory Light Chain Phospho. Other band(s): None.](http://images.novusbio.com/fullsize/MRCL3-[p-Ser19--p-Ser20]-Antibody-Western-Blot-NB100-1687-img0017.jpg)
![Western Blot: MRCL3 [p Ser19, p Ser20] Antibody [NB100-1687] - Used at a 1:1000 dilution to detect myosin light chain by Western blot on 3T3 cell lysates. A standard urea/glycerol gel without SDS was used to separate phospho forms of regulatory light chain according to mass to charge ratios. In Panel A, reactivity of phosphospecific antibody is shown. In Panel B, reactivity of commercially available pan reactive antibody that detects both unphosphorylated and phosphorylated forms of regulatory light chain is shown. phosphospecific antibody detects both monophosphorylated (pSer20 Mono-P-RLC) and diphosphorylated (pThr19-pSer20 Di-P-RLC) regulatory light chain.](http://images.novusbio.com/fullsize/MRCL3-[p-Ser19--p-Ser20]-Antibody-Western-Blot-NB100-1687-img0018.jpg)
![N/A Chemerin [HRP]](http://images.novusbio.com/fullsize2/DATA_Chemerin_MCHM00_ELISA_955.jpg "N/A Chemerin [HRP]")
![N/A Chemerin [HRP]](http://images.novusbio.com/fullsize2/Chemerin_MCHM00_ELISA_251.jpg)
![Western Blot: Nbs1 Antibody (7E4A2) [NBP2-26297] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Nbs1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Mouse Anti-Human Polyclonal Nbs1 Antibody [NBP2-26297] followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody [HAF018]. Specific band was detected for Nbs1 at approximately 95 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Nbs1-Antibody-7E4A2-Knockout-Validated-NBP2-26297-img0015.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Nbs1 Antibody (7E4A2) [NBP2-26297] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Nbs1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Mouse Anti-Human Polyclonal Nbs1 Antibody [NBP2-26297] followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody [HAF018]. Specific band was detected for Nbs1 at approximately 95 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Nbs1 Antibody (7E4A2) [NBP2-26297] - Analysis of Hela cells using mouse Nbs1 Antibody (7E4A2) [NBP2-26297] (green). Blue: DRAQ5 fluorescent DNA dye [NBP2-81125]. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/NBS1-Antibody-7E4A2-Immunocytochemistry-Immunofluorescence-NBP2-26297-img0008.jpg)
![Immunohistochemistry: Nbs1 Antibody (7E4A2) [NBP2-26297] - Analysis of paraffin-embedded rectum cancer tissues using mouse Nbs1 Antibody (7E4A2) [NBP2-26297] with DAB staining.](http://images.novusbio.com/fullsize/NBS1-Antibody-7E4A2-Immunohistochemistry-NBP2-26297-img0011.jpg)
![Flow Cytometry: Nbs1 Antibody (7E4A2) [NBP2-26297] - Analysis of Nbs1 in Hela cells using mouse Nbs1 Antibody (7E4A2) [NBP2-26297] (green) and negative control (red).](http://images.novusbio.com/fullsize/NBS1-Antibody-7E4A2-Flow-Cytometry-NBP2-26297-img0009.jpg)
![Immunohistochemistry-Paraffin: Nbs1 Antibody (7E4A2) [NBP2-26297] - Analysis of Nbs1 in paraffin-embedded cervical cancer tissues using mouse Nbs1 Antibody (7E4A2) [NBP2-26297] with DAB staining.](http://images.novusbio.com/fullsize/NBS1-Antibody-7E4A2-Immunohistochemistry-NBP2-26297-img0010.jpg)
![ELISA: Nbs1 Antibody (7E4A2) [NBP2-26297] - ELISA analysis using Nbs1 Antibody (7E4A2) [NBP2-26297].](http://images.novusbio.com/fullsize/Nbs1-Antibody-7E4A2-ELISA-NBP2-26297-img0014.jpg)
![Simple Western: Nbs1 Antibody (7E4A2) [NBP2-26297] - Analysis using Nbs1 Antibody (7E4A2) [NBP2-26297]. Molecular Weight observed at: 115 kDa.](http://images.novusbio.com/fullsize/Nbs1-Antibody-7E4A2-Simple-Western-NBP2-26297-img0017.jpg)
![N/A BMP-7 [Biotin]](http://images.novusbio.com/fullsize2/DATA_BMP7_DY354_ELISA_1956.jpg "N/A BMP-7 [Biotin]")
![N/A VEGF [HRP]](http://images.novusbio.com/fullsize2/DATA_VEGF_CAVE00_ELISA_525.jpg "N/A VEGF [HRP]")
![N/A VEGF [HRP]](http://images.novusbio.com/fullsize2/DATA_VEGF_CAVE00_ELISA_526.jpg)
![N/A VEGF [HRP]](http://images.novusbio.com/fullsize2/VEGF_CAVE00_ELISA_9.jpg)
![N/A Galectin-9 [Biotin]](http://images.novusbio.com/fullsize2/DATA_Galectin9_DY2045_ELISA_1755.jpg "N/A Galectin-9 [Biotin]")
![N/A IL-12 p70/IL-12A [HRP]](http://images.novusbio.com/fullsize2/DATA_IL12_p70_HS120_ELISA_884.jpg "N/A IL-12 p70/IL-12A [HRP]")
![N/A IL-12 p70/IL-12A [HRP]](http://images.novusbio.com/fullsize2/DATA_IL12_p70_HS120_ELISA_885.jpg)
![N/A IL-12 p70/IL-12A [HRP]](http://images.novusbio.com/fullsize2/IL-12_HS120_ELISA_214.jpg)
![N/A TNF-alpha [Biotin]](http://images.novusbio.com/fullsize2/DATA_TNFalpha_DY1814_ELISA_1720.jpg "N/A TNF-alpha [Biotin]")
![N/A Osteoactivin/GPNMB [Biotin]](http://images.novusbio.com/fullsize2/DATA_Osteoactivin_DY2330_ELISA_1813.jpg "N/A Osteoactivin/GPNMB [Biotin]")
![N/A Carbonic Anhydrase IX/CA9 [HRP]](http://images.novusbio.com/fullsize2/DATA_Carbonic_Anhydrase_IX_DCA900_ELISA_604.jpg "N/A Carbonic Anhydrase IX/CA9 [HRP]")
![N/A Carbonic Anhydrase IX/CA9 [HRP]](http://images.novusbio.com/fullsize2/Carbonic_Anhydrase_IX_DCA900_ELISA_48.jpg)
