HAF017). A specific band was detected for IGFBP-2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for IGFBP-2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of T47D human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IGFBP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF674) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for IGFBP-2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox"> HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

674-B2) inhibits Recombinant Human IGF-II (292-G2) induced proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IGF-II (14 ng/mL) activity elicited by Recombinant Human IGFBP-2 (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IGFBP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF674). The ND₅₀ is typically 2.5-7.5 µg/mL." class="big_lightbox"> 674-B2) inhibits Recombinant Human IGF-II (292-G2) induced proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IGF-II (14 ng/mL) activity elicited by Recombinant Human IGFBP-2 (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IGFBP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF674). The ND₅₀ is typically 2.5-7.5 µg/mL." class="big_thumb" />

674-B2) was serially diluted and captured by Mouse Anti-Human IGFBP‑2 Monoclonal Antibody (Catalog # MAB6741) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IGFBP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF674) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 674-B2) was serially diluted and captured by Mouse Anti-Human IGFBP‑2 Monoclonal Antibody (Catalog # MAB6741) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IGFBP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF674) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

797-B2) was serially diluted and captured by Rat Anti-Mouse IGFBP‑2 Monoclonal Antibody (Catalog # MAB7971) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IGFBP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF797) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 797-B2) was serially diluted and captured by Rat Anti-Mouse IGFBP‑2 Monoclonal Antibody (Catalog # MAB7971) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IGFBP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF797) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Mouse IGFBP‑2 (Catalog # 797-B2) was serially diluted and captured by Rat Anti-Mouse IGFBP‑2 Monoclonal Antibody (Catalog # MAB7971) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IGFBP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF797) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

797-B2) was serially diluted and captured by Rat Anti-Mouse IGFBP‑2 Monoclonal Antibody (Catalog # MAB7971) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IGFBP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF797) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 797-B2) was serially diluted and captured by Rat Anti-Mouse IGFBP‑2 Monoclonal Antibody (Catalog # MAB7971) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IGFBP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF797) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Mouse IGFBP‑2 (Catalog # 797-B2) was serially diluted and captured by Rat Anti-Mouse IGFBP‑2 Monoclonal Antibody (Catalog # MAB7971) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IGFBP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF797) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

674-B2) was serially diluted and captured by Mouse Anti-Human IGFBP‑2 Monoclonal Antibody (Catalog # MAB6741) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IGFBP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF674) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 674-B2) was serially diluted and captured by Mouse Anti-Human IGFBP‑2 Monoclonal Antibody (Catalog # MAB6741) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IGFBP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF674) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

797-B2) inhibits Recombinant Mouse IGF-II (Catalog # 792-MG) induced proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IGF-II (30 ng/mL) activity elicited by Recombinant Mouse IGFBP-2 (0.75 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IGFBP-2 Monoclonal Antibody (Catalog # MAB797). The ND₅₀ is typically 5-20 µg/mL." class="big_lightbox"> 797-B2) inhibits Recombinant Mouse IGF-II (Catalog # 792-MG) induced proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IGF-II (30 ng/mL) activity elicited by Recombinant Mouse IGFBP-2 (0.75 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IGFBP-2 Monoclonal Antibody (Catalog # MAB797). The ND₅₀ is typically 5-20 µg/mL." alt="Recombinant Mouse IGFBP-2 (Catalog # 797-B2) inhibits Recombinant Mouse IGF-II (Catalog # 792-MG) induced proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IGF-II (30 ng/mL) activity elicited by Recombinant Mouse IGFBP-2 (0.75 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IGFBP-2 Monoclonal Antibody (Catalog # MAB797). The ND₅₀ is typically 5-20 µg/mL." class="big_thumb" />