Antibody database

Glial fibrillary acidic protein (GFAP) is the main intermediate filament protein in mature astrocytes, but also an important component of the cytoskeleton in astrocytes during development. Recent developments using GFAP antibodies in astrocyte biology, and the discovery of novel intermediate filament functions, have enticed interest in the function of GFAP.

The transcription factor beta protein 1 (BP1) is a member of the homeobox gene family and the distal-less subfamily. Expression of BP1 is highly tissue-specific and developmentally restricted. Among different human tissues, BP1 is found to be highly expressed in placenta, kidney and at lower levels in fetal liver (1). Such restricted pattern of expression is compatible with a specific gene function in development and/or differentiation.

Reactive oxygen species, ROS, are beneficially involved in many signaling pathways that control development and maintain cellular homeostasis. In physiological conditions, a tightly regulated redox balance protects cells from injurious ROS activity, altered balance leads to various pathological conditions including cancer. MAP17 is a small 17-kDa non-glycosylated membrane protein that is overexpressed in many tumors of different origins, including carcinomas.

Epithelial-to-mesenchymal transition (EMT) is a critical event in the induction of cell motility and increased survival both under physiological situations like wound healing or development, as well as in malignant cells undergoing invasion and metastasis. Vimentin is an intermediate filament protein which is characteristically upregulated in cells undergoing EMT. Recent studies support the notion that vimentin functions as a positive regulator of EMT and upregulation of vimentin appears to be a prerequisite for EMT induction (1).

I began using the HSP60 antibody (NB110-57063) in June of 2010 and it worked well. I do not like to buy antibodies that have not been tested in the species for which I will use them, so I picked this antibody because it had already been tested in rat tissue. I split the antibody into 20ul aliquots and stored it at -20C. I first ran a Western blot with 15ug of a RIPA whole cell lystate from WKPT cells a rat kidney immortalized cell line derived from the S1 proximal tubule segment.

Apoptosis is one of the main types of programmed cell death which involves a cascade of biochemical events leading to specific cell morphology characteristics and ultimately death of cells.

S100A12 (Calgranulin C) belongs to the S100 family of calcium-binding proteins. The 20 members of this group share EF-hand domains which are involved in binding of calcium. S100A12 is expressed by granulocytes, whereas its expression by monocytes remains controversial (1). S100A12 is secreted by activated granulocytes (2). S100A12 is a ligand for the receptor for advanced glycation end products (RAGE) expressed on macrophages, lymphocytes and endothelium.

I first tried the PBP antibody (NB110-93495) in June of 2010 and it worked well. I picked this antibody because it had been tested in rat tissue, so I was confident it would work for my rat samples. I stored the PBP antibody in 20ul aliquots after it arrived then stored it at -20C and used it over a 3 month period. I ran a Western blot with 15ug of a RIPA whole cell lystate from WKPT cells a rat kidney immortalized cell line derived from the S1 proximal tubule segment.

Triacylglycerols (triglycerides) (TGs) are the major storage molecules of metabolic energy and FAs in most living organisms. Excessive accumulation of TGs, however, is associated with human diseases, such as obesity, diabetes mellitus, and steatohepatitis. The final and the only committed step in the biosynthesis of TGs is catalyzed by acyl-CoA: diacylglycerol acyltransferase (DGAT) enzymes.

Apolipoprotein E also known as ApoE is a 36kDa protein that is expressed in all lipoprotein fractions in plasma. This protein is produced in high quantities in the liver, brain, spleen, lung and kidney. The function of APOE is to mediate the binding, internalize and catabolize lipoprotein particles. A study carried out by researchers at the Case Western Reserve University in Ohio has recently been highlighted in the BBC Health News.