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![Western Blot: RNF139 Antibody [NBP1-23027] - (1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/RNF139-Antibody-Western-Blot-NBP1-23027-img0005.jpg "Western Blot: RNF139 Antibody [NBP1-23027] - (1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: RNF139 Antibody [NBP1-23027] - (3.8ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/RNF139-Antibody-Immunohistochemistry-Paraffin-NBP1-23027-img0004.jpg)
![Western Blot: Livin Antibody [NB300-941] - (0.3ug/ml) of MOLT4 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.](http://images.novusbio.com/fullsize/Livin-Antibody-Western-Blot-NB300-941-img0002.jpg "Western Blot: Livin Antibody [NB300-941] - (0.3ug/ml) of MOLT4 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.")
![Immunohistochemistry-Paraffin: Axotrophin Antibody [NB100-93417] - (3.75ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Axotrophin-Antibody-Immunohistochemistry-Paraffin-NB100-93417-img0001.jpg "Immunohistochemistry-Paraffin: Axotrophin Antibody [NB100-93417] - (3.75ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Western Blot: RNF12 Antibody [H00051132-B01P] - Analysis of RNF12 expression in transfected 293T cell line by RNF12 polyclonal antibody. Lane 1: RNF12 transfected lysate(68.64 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/RNF12-Antibody-Western-Blot-H00051132-B01P-img0002.jpg "Western Blot: RNF12 Antibody [H00051132-B01P] - Analysis of RNF12 expression in transfected 293T cell line by RNF12 polyclonal antibody. Lane 1: RNF12 transfected lysate(68.64 KDa). Lane 2: Non-transfected lysate.")
![Western Blot: TRIM9 Antibody (1F12) [H00114088-M01] - Analysis of TRIM9 expression in transfected 293T cell line by TRIM9 monoclonal antibody (M01), clone 1F12.Lane 1: TRIM9 transfected lysate(61.3 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/TRIM9-Antibody-1F12-Western-Blot-H00114088-M01-img0002.jpg "Western Blot: TRIM9 Antibody (1F12) [H00114088-M01] - Analysis of TRIM9 expression in transfected 293T cell line by TRIM9 monoclonal antibody (M01), clone 1F12.Lane 1: TRIM9 transfected lysate(61.3 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: Axotrophin Antibody (2B9) [H00064844-M01] - MARCH7 monoclonal antibody (M01), clone 2B9 Analysis of MARCH7expression in K-562.](http://images.novusbio.com/fullsize/Axotrophin-Antibody-2B9-Western-Blot-H00064844-M01-img0007.jpg "Western Blot: Axotrophin Antibody (2B9) [H00064844-M01] - MARCH7 monoclonal antibody (M01), clone 2B9 Analysis of MARCH7expression in K-562.")
![Immunocytochemistry/Immunofluorescence: Axotrophin Antibody (2B9) [H00064844-M01] - Analysis of monoclonal antibody to MARCH7 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Axotrophin-Antibody-2B9-Immunocytochemistry-Immunofluorescence-H00064844-M01-img0005.jpg)
![ELISA: Axotrophin Antibody (2B9) [H00064844-M01] - Detection limit for recombinant GST tagged MARCH7 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Axotrophin-Antibody-2B9-Sandwich-ELISA-H00064844-M01-img0006.jpg)
![Western Blot: RNF98 Antibody [NBP1-33644] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/RNF98-Antibody-Western-Blot-NBP1-33644-img0003.jpg "Western Blot: RNF98 Antibody [NBP1-33644] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE; antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: RNF98 Antibody [NBP1-33644] - Analysis of methanol-fixed A549, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/RNF98-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33644-img0002.jpg)
![Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with TRIM36 antibody [C3], C-term (NBP1-33644) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33644_rabbit-polyclonal-rnf98-antibody-western-blot-261120251825317.jpg)
![Immunohistochemistry-Paraffin: TRIM8 Antibody [NBP2-16642] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/GERP-Antibody-Immunohistochemistry-Paraffin-NBP2-16642-img0002.jpg "Immunohistochemistry-Paraffin: TRIM8 Antibody [NBP2-16642] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: TRIM8 Antibody [NBP2-16642] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GERP antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-16642_rabbit-polyclonal-trim8-antibody-1610202419414593.jpg)
![Immunocytochemistry/Immunofluorescence: MuRF1/TRIM63 Antibody [NBP3-23425] - Immunocytochemical labeling of MuRF1/TRIM63 in mouse C2C12 cells. The cells were labeled with rabbit polyclonal MuRF1/TRIM63 antibody, then detected using appropriate secondary antibody conjugated to Cy3. The antibody was used in the absence (left) or presence (right) of blocking peptide.](http://images.novusbio.com/fullsize/nbp3-23425_rabbit-murf1-trim63-pab-19920231410120.jpg "Immunocytochemistry/Immunofluorescence: MuRF1/TRIM63 Antibody [NBP3-23425] - Immunocytochemical labeling of MuRF1/TRIM63 in mouse C2C12 cells. The cells were labeled with rabbit polyclonal MuRF1/TRIM63 antibody, then detected using appropriate secondary antibody conjugated to Cy3. The antibody was used in the absence (left) or presence (right) of blocking peptide.")
![Western Blot: MuRF1/TRIM63 Antibody [NBP3-23425] - Western blot analysis of mouse heart tissue (lanes 1 & 3) or C2C12 cells (lanes 2 & 4). The blot was probed with anti-MuRF1/TRIM63 (C-terminal region) (lanes 1 & 2) or anti-Fbx32 (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23425_rabbit-murf1-trim63-pab-19920231410118.jpg)
![Western Blot: RNF6 Antibody [NBP1-80251] - Titration: 1.25ug/ml Positive Control: SP2/0 cell lysate.](http://images.novusbio.com/fullsize/RNF6-Antibody-Western-Blot-NBP1-80251-img0004.jpg "Western Blot: RNF6 Antibody [NBP1-80251] - Titration: 1.25ug/ml Positive Control: SP2/0 cell lysate.")
![Immunohistochemistry-Paraffin: RNF6 Antibody [NBP1-80251] - Mouse intestine, 4-8ug/ml.](http://images.novusbio.com/fullsize/RNF6-Antibody-Immunohistochemistry-Paraffin-NBP1-80251-img0001.jpg)
![Immunohistochemistry-Paraffin: RNF6 Antibody [NBP1-80251] - Mouse Pancreas tissue, islet cell (Indicated with Arrows) 4-8ug/ml.](http://images.novusbio.com/fullsize/RNF6-Antibody-Immunohistochemistry-Paraffin-NBP1-80251-img0003.jpg)
![Western Blot: TRIM34 Antibody [NBP1-80359] - Human Fetal Muscle, Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/TRIM34-Antibody-Western-Blot-NBP1-80359-img0005.jpg "Western Blot: TRIM34 Antibody [NBP1-80359] - Human Fetal Muscle, Antibody Dilution: 1.0 ug/ml.")
![Western Blot: TRIM34 Antibody [NBP1-80359] - Human Thymus lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/TRIM34-Antibody-Western-Blot-NBP1-80359-img0002.jpg)
![Western Blot: TRIM34 Antibody [NBP1-80359] - Human Adult Placenta, Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/TRIM34-Antibody-Western-Blot-NBP1-80359-img0003.jpg)
![Western Blot: TRIM34 Antibody [NBP1-80359] - Human Fetal Lung, Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/TRIM34-Antibody-Western-Blot-NBP1-80359-img0004.jpg)
![Western Blot: NFX1 Antibody [NBP1-49933] - Detection of Human and Mouse NFX1 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-NFX1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. NFX1 was also immunoprecipitated by rabbit anti-NFX1 antibodies which recognize upstream epitopes. For blotting immunoprecipitated NFX1 was used. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/NFX1-Antibody-Western-Blot-NBP1-49933-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: NFX1 Antibody [NBP1-49933] - Detection of Human and Mouse NFX1 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-NFX1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. NFX1 was also immunoprecipitated by rabbit anti-NFX1 antibodies which recognize upstream epitopes. For blotting immunoprecipitated NFX1 was used. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).")