| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98754-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90139-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98826-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98881-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89251-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99858-50ul | |||
![Immunoprecipitation: p53 Antibody (PSH03-95) [NBP3-32697] - p53 was immunoprecipitated in 0.2mg NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate with NBP3-32697 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32697 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate (input) Lane 2: NBP3-32697 IP in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate Lane 3: Rabbit IgG instead of NBP3-32697 in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes](http://images.novusbio.com/fullsize/nbp3-32697_rabbit-p53-mab-psh03-95-26820241582614.jpg "Immunoprecipitation: p53 Antibody (PSH03-95) [NBP3-32697] - p53 was immunoprecipitated in 0.2mg NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate with NBP3-32697 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32697 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate (input) Lane 2: NBP3-32697 IP in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate Lane 3: Rabbit IgG instead of NBP3-32697 in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes")
![Western Blot: p53 Antibody (PSH03-95) [NBP3-32697] - Western blot analysis of p53 on different lysates with Rabbit anti-p53 antibody (NBP3-32697) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: Neuro-2a cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 0.5uM doxorubicincell for 24 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 3 minutes 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32697) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32697_rabbit-p53-mab-psh03-95-278202415212448.jpg)
![Immunocytochemistry/ Immunofluorescence: p53 Antibody (PSH03-95) [NBP3-32697] - Immunocytochemistry analysis of NIH/3T3 cells treated with 0.5μM doxorubicin for 20 hours labeling p53 with Rabbit anti-p53 antibody (NBP3-32697) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p53 antibody (NBP3-32697) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32697_rabbit-p53-mab-psh03-95-2920241048481.jpg)
![Immunohistochemistry-Paraffin: Carm1 Antibody [NBP2-98754] - Immunochemical staining of human Carm1 in human ovarian cancer with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Carm1-Antibody-Immunohistochemistry-Paraffin-NBP2-98754-img0003.jpg "Immunohistochemistry-Paraffin: Carm1 Antibody [NBP2-98754] - Immunochemical staining of human Carm1 in human ovarian cancer with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: Carm1 Antibody [NBP2-98754] - Immunochemical staining of human Carm1 in human prostatic carcinoma with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Carm1-Antibody-Immunohistochemistry-Paraffin-NBP2-98754-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Carm1 Antibody [NBP2-98754] - Immunofluorescence staining of Carm1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98754 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-98754_rabbit-polyclonal-carm1-antibody-23520251628120.png)
![Immunohistochemistry: Bcl-6 Antibody (A6C7-R) [NBP3-32074] - Immunohistochemical analysis of paraffin-embedded human B lymphocytoma tissue with Mouse anti-Bcl-6 antibody (NBP3-32074) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32074) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32074_mouse-bcl-6-mab-a6c7-r-29820241420947.jpg "Immunohistochemistry: Bcl-6 Antibody (A6C7-R) [NBP3-32074] - Immunohistochemical analysis of paraffin-embedded human B lymphocytoma tissue with Mouse anti-Bcl-6 antibody (NBP3-32074) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32074) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: Bcl-6 Antibody (A6C7-R) [NBP3-32074] - Immunocytochemistry analysis of Raji cells labeling Bcl-6 with Mouse anti-Bcl-6 antibody (NBP3-32074) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Bcl-6 antibody (NBP3-32074) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32074_mouse-bcl-6-mab-a6c7-r-2920241114839.jpg)
![Western Blot: TRADD Antibody (JE32-60) [NBP3-33041] - Western blot analysis of TRADD on different lysates with Rabbit anti-TRADD antibody (NBP3-33041) at 1/1,000 dilution. Lane 1: HL-60 cell lysate Lane 2: HeLa cell lysate Lane 3: K-562 cell lysate Lane 4: Ramos cell lysate Lane 5: Jurkat cell lysate Lane 6: C2C12 cell lysate Lane 7: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 2 minutes 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33041) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33041_rabbit-tradd-mab-je32-60-28820241249346.jpg "Western Blot: TRADD Antibody (JE32-60) [NBP3-33041] - Western blot analysis of TRADD on different lysates with Rabbit anti-TRADD antibody (NBP3-33041) at 1/1,000 dilution. Lane 1: HL-60 cell lysate Lane 2: HeLa cell lysate Lane 3: K-562 cell lysate Lane 4: Ramos cell lysate Lane 5: Jurkat cell lysate Lane 6: C2C12 cell lysate Lane 7: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 2 minutes 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33041) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: TRADD Antibody (JE32-60) [NBP3-33041] - Immunocytochemistry analysis of MCF7 cells labeling TRADD with Rabbit anti-TRADD antibody (NBP3-33041) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TRADD antibody (NBP3-33041) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33041_rabbit-tradd-mab-je32-60-29202410334011.jpg)
![Western Blot: MSH2 Antibody (10G2-R) [NBP3-32605] - Western blot analysis of MSH2 on different lysates with Mouse anti-MSH2 antibody (NBP3-32605) at 1/1,000 dilution. Lane 1: HeLa cell lysate (15 ug/Lane) Lane 2: HEK-293 cell lysate (15 ug/Lane) Lane 3: A549 cell lysate (15 ug/Lane) Lane 4: A431 cell lysate (15 ug/Lane) Lane 5: HCT 116 cell lysate (15 ug/Lane) Lane 6: SW480 cell lysate (15 ug/Lane) Lane 7: PC-3M cell lysate (15 ug/Lane) Lane 8: LNCaP cell lysate (negative) (15 ug/Lane) Lane 9: LoVo cell lysate (negative) (15 ug/Lane) Lane 10: NIH/3T3 cell lysate (15 ug/Lane) Lane 11: RAW264.7 cell lysate (15 ug/Lane) Lane 12: PC-12 cell lysate (15 ug/Lane) Lane 13: Mouse testis tissue lysate (25 ug/Lane) Lane 14: Rat testis tissue lysate (25 ug/Lane) Predicted band size: 105 kDa Observed band size: 105 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32605) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32605_mouse-msh2-mab-10g2-r-27820241631687.jpg "Western Blot: MSH2 Antibody (10G2-R) [NBP3-32605] - Western blot analysis of MSH2 on different lysates with Mouse anti-MSH2 antibody (NBP3-32605) at 1/1,000 dilution. Lane 1: HeLa cell lysate (15 ug/Lane) Lane 2: HEK-293 cell lysate (15 ug/Lane) Lane 3: A549 cell lysate (15 ug/Lane) Lane 4: A431 cell lysate (15 ug/Lane) Lane 5: HCT 116 cell lysate (15 ug/Lane) Lane 6: SW480 cell lysate (15 ug/Lane) Lane 7: PC-3M cell lysate (15 ug/Lane) Lane 8: LNCaP cell lysate (negative) (15 ug/Lane) Lane 9: LoVo cell lysate (negative) (15 ug/Lane) Lane 10: NIH/3T3 cell lysate (15 ug/Lane) Lane 11: RAW264.7 cell lysate (15 ug/Lane) Lane 12: PC-12 cell lysate (15 ug/Lane) Lane 13: Mouse testis tissue lysate (25 ug/Lane) Lane 14: Rat testis tissue lysate (25 ug/Lane) Predicted band size: 105 kDa Observed band size: 105 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32605) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MSH2 Antibody (10G2-R) [NBP3-32605] - Immunohistochemical analysis of paraffin-embedded human appendix tissue with Mouse anti-MSH2 antibody (NBP3-32605) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32605) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32605_mouse-msh2-mab-10g2-r-29820241492619.jpg)
![Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody (012) [NBP2-90139] - Immunochemical staining of human SOD2 in human breast-carcinoma with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-012-Immunohistochemistry-Paraffin-NBP2-90139-img0004.jpg "Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody (012) [NBP2-90139] - Immunochemical staining of human SOD2 in human breast-carcinoma with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody (012) [NBP2-90139] - Immunochemical staining of human SOD2 in human placenta with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-012-Immunohistochemistry-Paraffin-NBP2-90139-img0001.jpg)
![Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody (012) [NBP2-90139] - Immunochemical staining of human SOD2 in human kidney with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-012-Immunohistochemistry-Paraffin-NBP2-90139-img0002.jpg)
![Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody (012) [NBP2-90139] - Immunochemical staining of human SOD2 in human colon-carcinoma with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-012-Immunohistochemistry-Paraffin-NBP2-90139-img0003.jpg)
![Western Blot: CDC73/HRPT2 Antibody (JE66-42) [NBP3-32442] - Western blot analysis of CDC73/HRPT2 on different lysates with Rabbit anti-CDC73/HRPT2 antibody (NBP3-32442) at 1/1,000 dilution. Lane 1: SW480 cell lysate Lane 2: K-562 cell lysate Lane 3: HeLa cell lysate Lane 4: Jurkat cell lysate Lane 5: HepG2 cell lysate Lane 6: MCF7 cell lysate Lane 7: HEK-293 cell lysate Lane 8: RAW264.7 cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32442) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32442_rabbit-cdc73-hrpt2-mab-je66-42-278202415295587.jpg "Western Blot: CDC73/HRPT2 Antibody (JE66-42) [NBP3-32442] - Western blot analysis of CDC73/HRPT2 on different lysates with Rabbit anti-CDC73/HRPT2 antibody (NBP3-32442) at 1/1,000 dilution. Lane 1: SW480 cell lysate Lane 2: K-562 cell lysate Lane 3: HeLa cell lysate Lane 4: Jurkat cell lysate Lane 5: HepG2 cell lysate Lane 6: MCF7 cell lysate Lane 7: HEK-293 cell lysate Lane 8: RAW264.7 cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32442) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CDC73/HRPT2 Antibody (JE66-42) [NBP3-32442] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CDC73/HRPT2 antibody (NBP3-32442) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32442) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32442_rabbit-cdc73-hrpt2-mab-je66-42-298202414304141.jpg)
![Western Blot: TLK1 Antibody [NBP2-98826] - Lane A: 293T Whole Cell LysateLane B: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:87 kDaObserved band size:87 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/TLK1-Antibody-Western-Blot-NBP2-98826-img0003.jpg "Western Blot: TLK1 Antibody [NBP2-98826] - Lane A: 293T Whole Cell LysateLane B: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:87 kDaObserved band size:87 kDa(We are unsure as to the identity of these extra bands.)")
![Immunoprecipitation: TLK1 Antibody [NBP2-98826] - Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg K562 Whole Cell Lysate4 uL anti-TLK1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-TLK1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 87 kDaObserved band size: 87 kDa](http://images.novusbio.com/fullsize/TLK1-Antibody-Immunoprecipitation-NBP2-98826-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: TLK1 Antibody [NBP2-98826] - Immunofluorescence staining of TLK1 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-98826 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize/nbp2-98826_rabbit-polyclonal-tlk1-antibody-235202511354115.png)
![Western Blot: p16INK4a/CDKN2A Antibody [NBP2-98881] - Anti-p16INK4a/CDKN2A rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 17 kDa](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Western-Blot-NBP2-98881-img0005.jpg "Western Blot: p16INK4a/CDKN2A Antibody [NBP2-98881] - Anti-p16INK4a/CDKN2A rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 17 kDa")
![Immunoprecipitation: p16INK4a/CDKN2A Antibody [NBP2-98881] - p16INK4a/CDKN2A was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg 293T Whole Cell Lysate 2 ul anti-p16INK4a/CDKN2A rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-p16INK4a/CDKN2A rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 17 kDa](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Immunoprecipitation-NBP2-98881-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: p16INK4a/CDKN2A Antibody [NBP2-98881] - Immunofluorescence staining of p16INK4a/CDKN2A in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98881 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-98881_rabbit-polyclonal-p16ink4a-cdkn2a-antibody-235202512205526.png)
![Western Blot: SMAC/Diablo Antibody (006) [NBP2-89251] - NBP2-89251 at 1:10000 dilution Lane A: Hela Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: A431 Whole Cell Lysate Lane D: MCF7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:22 kDa Observed band size:20 kDa](http://images.novusbio.com/fullsize/nbp2-89251_rabbit-monoclonal-smac-diablo-antibody-006-23520251129339.png "Western Blot: SMAC/Diablo Antibody (006) [NBP2-89251] - NBP2-89251 at 1:10000 dilution Lane A: Hela Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: A431 Whole Cell Lysate Lane D: MCF7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:22 kDa Observed band size:20 kDa")
![Flow Cytometry: SMAC/Diablo Antibody (006) [NBP2-89251] - Flow cytometric analysis of Human SMAC/Diablo expression on HeLa cells. The cells were treated, stained with NBP2-89251 (Filled histogram), then a FITC-conjugated second step antibody. To demonstrate specificity of staining, the binding by NBP2-89251 was blocked by preincubation of the purified antibody with 20ug recombinant human SMAC/Diablo for 1 hour (Black solid line hisgram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/nbp2-89251_rabbit-monoclonal-smac-diablo-antibody-006-235202514492353.png)
![Immunocytochemistry/ Immunofluorescence: SMAC/Diablo Antibody (006) [NBP2-89251] - Immunofluorescence staining of Human SMAC/Diablo in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89251 (1:60) at 4 overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89251_rabbit-monoclonal-smac-diablo-antibody-006-235202511444460.png)
![Western Blot: Protein Kinase D2 Antibody [NBP2-99858] - Anti-Protein Kinase D2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 97 kDa. Observed band size: 113 kDa](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-Western-Blot-NBP2-99858-img0004.jpg "Western Blot: Protein Kinase D2 Antibody [NBP2-99858] - Anti-Protein Kinase D2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 97 kDa. Observed band size: 113 kDa")
![Immunoprecipitation: Protein Kinase D2 Antibody [NBP2-99858] - Protein Kinase D2 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate Lane B: 0.5 mg HepG2 Whole Cell Lysate Lane C: 0.5 mg MCF-7 Whole Cell Lysate 4 ul anti-Protein Kinase D2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Protein Kinase D2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 100 kDa. Observed band size: 100 kDa](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-Immunoprecipitation-NBP2-99858-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Protein Kinase D2 Antibody [NBP2-99858] - Immunofluorescence staining of Protein Kinase D2 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99858 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-99858_rabbit-polyclonal-protein-kinase-d2-antibody-235202521525037.png)
![Western Blot: p53 Antibody (6B5-R) [NBP3-32698] - Western blot analysis of p53 on different lysates with Mouse anti-p53 antibody (NBP3-32698) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: MCF7 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 0.5uM doxorubicincell for 24 hours lysate Lane 5: Neuro-2a cell lysate Lane 6: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: Lane 1-2: 1 minute 40 seconds; Lane 3-6: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32698) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32698_mouse-p53-mab-6b5-r-278202415212411.jpg "Western Blot: p53 Antibody (6B5-R) [NBP3-32698] - Western blot analysis of p53 on different lysates with Mouse anti-p53 antibody (NBP3-32698) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: MCF7 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 0.5uM doxorubicincell for 24 hours lysate Lane 5: Neuro-2a cell lysate Lane 6: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: Lane 1-2: 1 minute 40 seconds; Lane 3-6: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32698) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: p53 Antibody (6B5-R) [NBP3-32698] - Immunocytochemistry analysis of A431 cells labeling p53 with Mouse anti-p53 antibody (NBP3-32698) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p53 antibody (NBP3-32698) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32698_mouse-p53-mab-6b5-r-29202410484875.jpg)