| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92850-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50538-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56100SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56081SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-24574-25ul | |||
| Azide and BSA Free | NBP3-43853 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-24573-25ul | |||
| Azide and BSA Free | NBP3-43852 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-24572-25ul | |||
| Azide and BSA Free | NBP3-43851 | |||
![Western Blot: DDX3 Antibody [NBP2-92850] - nalysis of extracts of various cell lines, using DDX3Y at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.](http://images.novusbio.com/fullsize/DDX3-Antibody-Western-Blot-NBP2-92850-img0008.jpg "Western Blot: DDX3 Antibody [NBP2-92850] - nalysis of extracts of various cell lines, using DDX3Y at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: DDX3 Antibody [NBP2-92850] - Analysis of U-2 OS cells using DDX3Y . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92850-img0003.jpg)
![Immunohistochemistry-Paraffin: DDX3 Antibody [NBP2-92850] - Rat testis using DDX3Y antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-Paraffin-NBP2-92850-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: DDX3 Antibody [NBP2-92850] - Analysis of C6 cells using DDX3Y . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92850-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: DDX3 Antibody [NBP2-92850] - Analysis of L929 cells using DDX3Y . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92850-img0002.jpg)
![Immunohistochemistry-Paraffin: DDX3 Antibody [NBP2-92850] - Rat brain using DDX3Y antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-Paraffin-NBP2-92850-img0004.jpg)
![Immunohistochemistry-Paraffin: DDX3 Antibody [NBP2-92850] - Mouse heart using DDX3Y antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-Paraffin-NBP2-92850-img0005.jpg)
![Immunohistochemistry-Paraffin: DDX3 Antibody [NBP2-92850] - Mouse brain using DDX3Y antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-Paraffin-NBP2-92850-img0006.jpg)
![Western Blot: p53 Antibody (Pab DO-1) [NBP2-50538] - B) Western Blot analyses of RPMI-8402 and NALM-6 cell lines treated for 24 h with AZD-1775 (185 nM) and PF-00477736 (25 and 250 nM respectively). B-actin was used for loading normalization. For relative quantification of each protein see Figure S3A and for whole western blot images see Figure S6. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/11/11/1654) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/p53-Antibody-Pab-DO-1-Western-Blot-NBP2-50538-img0001.jpg "Western Blot: p53 Antibody (Pab DO-1) [NBP2-50538] - B) Western Blot analyses of RPMI-8402 and NALM-6 cell lines treated for 24 h with AZD-1775 (185 nM) and PF-00477736 (25 and 250 nM respectively). B-actin was used for loading normalization. For relative quantification of each protein see Figure S3A and for whole western blot images see Figure S6. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/11/11/1654) licensed under a CC-BY license.")
![Western Blot: p53 Antibody (Pab DO-1) - BSA Free [NBP2-50538] - DCQ reduces HIF-1 alpha through different mechanisms in MCF-7 & MDA-MB-231. (A) MCF-7 cells were transfected with siRNA against p53 & ctrl siRNA (scrambled sequence) using lipofectamine 2000. After 24 hours, cells were treated with DCQ (5 μM) for 6 hours under hypoxia. Whole cell lysates were prepared, & blots were probed against indicated antibodies. (B) MCF-7 cells were transfected with siRNA against p53 & ctrl siRNA (scrambled sequence) using lipofectamine 2000. Transfected cells were treated with DCQ (5 μM) for 6 hours under hypoxia. The extent of DNA fragmentation was determined by TUNEL assay 24 hours later using flow cytometry. One-way ANOVA was used to compare DCQ-treated versus control & statistical significance of p < 0.05 is indicated by *. (C) Whole cell lysates were prepared after pretreating MCF-7 & MDA-MB-231 with the proteasome inhibitor MG132 (3 μM) then treated with DCQ, & blots were probed for HIF-1 alpha . Results are from three independent experiments. (D) Whole cell lysates of MCF-7 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for p-AKT, mTOR, p-mTOR & GAPDH. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-12), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-310202416114753.jpg)
![Western Blot: p53 Antibody (Pab DO-1) - BSA Free [NBP2-50538] - DCQ induces DNA damage & apoptosis via reactive oxygen species. (A) Whole cell lysates of MCF-7 & MDA-MB-231 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for p-H2AX, p53, p-p53, p21, HIF-1 alpha & GAPDH. Results are representative of three independent experiments. (B) MDA-MB-231 & MCF-7 cells were pretreated with 1 mM Vitamin E or DTT for 2 hours followed by 25 min incubation with 10 μM CM-H2DCFDA dye. Cells were washed with PBS & treated with DCQ for 1 hour under normoxia or hypoxia, after which cells were harvested & the amount of DCF fluorescence was analyzed by flow cytometry. Each percentage is the average ± SE of three independent experiments. (C) MDA-MB-231 & MCF-7 cells were pretreated with 1 mM Vitamin E or DTT for 2 hours followed by 6 hours treatment with DCQ under normoxia or hypoxia. After 24 hours, the extent of DNA fragmentation was determined by TUNEL assay & measured by flow cytometry. One-way ANOVA was used to compare DCQ-treated versus control & statistical significance of p < 0.05 is indicated by *. (D) Whole cell lysates of MCF-7 & MDA-MB-231 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for HIF-1 alpha & GAPDH. Results are representative of three independent experiments. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-12), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-31020241612246.jpg)
![Flow Cytometry: Mouse Monoclonal p53 Antibody (Pab DO-1) [NBP2-50538] - P53 expression in monocyte subset of Human PBMCs post IFN gamma treatment. Image from a verified customer review. The customer used the Alexa Fluor® 647-conjugated version of NBP2-50538, which is listed under NBP2-50538AF647.](http://images.novusbio.com/fullsize/antibody/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-flow-cytometry-152025141858..png)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody [NB100-56100] - FFPE section from a secondary follicle of the mouse lymph node stained for Bcl-2 expression using NB100-56100 at 1:2000. A, Abundant staining is seen in the mantle zone, and little staining is seen in the germinal center. (A1) is a higher magnification of (A).](http://images.novusbio.com/fullsize/Bcl-2-Antibody-Immunohistochemistry-Paraffin-NB100-56100-img0002.jpg "Immunohistochemistry-Paraffin: Bcl-2 Antibody [NB100-56100] - FFPE section from a secondary follicle of the mouse lymph node stained for Bcl-2 expression using NB100-56100 at 1:2000. A, Abundant staining is seen in the mantle zone, and little staining is seen in the germinal center. (A1) is a higher magnification of (A).")
![Immunohistochemistry-Paraffin: Bag-1 Antibody [NB100-56081] - Formalin-fixed, paraffin-embedded sections of human colorectal cancer stained for Bag-1 using this antibody at 1:2000. Hematoxylin-eosin counterstain. Samples from two patients (A and B) are shown. A1 and B1 are higher magnifications from A and B, respectively.](http://images.novusbio.com/fullsize/Bag-1-Antibody-Immunohistochemistry-Paraffin-NB100-56081-img0004.jpg "Immunohistochemistry-Paraffin: Bag-1 Antibody [NB100-56081] - Formalin-fixed, paraffin-embedded sections of human colorectal cancer stained for Bag-1 using this antibody at 1:2000. Hematoxylin-eosin counterstain. Samples from two patients (A and B) are shown. A1 and B1 are higher magnifications from A and B, respectively.")
![Immunohistochemistry-Paraffin: Bag-1 Antibody [NB100-56081] - Formalin-fixed, paraffin-embedded section of human colon stained for Bag-1 using this antibody at 1:2000. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/Bag-1-Antibody-Immunohistochemistry-Paraffin-NB100-56081-img0003.jpg)
![Western Blot: Cyclin B1 Antibody (1C8) [H00000891-M01] - CCNB1 monoclonal antibody (M01), clone 1C8 Analysis of CCNB1 expression in JAR.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-1C8-Western-Blot-H00000891-M01-img0007.jpg "Western Blot: Cyclin B1 Antibody (1C8) [H00000891-M01] - CCNB1 monoclonal antibody (M01), clone 1C8 Analysis of CCNB1 expression in JAR.")
![Immunohistochemistry-Paraffin: Cyclin B1 Antibody (1C8) [H00000891-M01] - Analysis of monoclonal antibody to Cyclin B1 on formalin-fixed paraffin-embedded human tonsil. Antigen retrieval was done enzymatically. Primary antibody dilution was 1:100. Image from verified customer review.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-1C8-Immunohistochemistry-Paraffin-H00000891-M01-img0009.jpg)
![Immunohistochemistry-Paraffin: Cyclin B1 Antibody (1C8) [H00000891-M01] - Analysis of monoclonal antibody to CCNB1 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-1C8-Immunohistochemistry-Paraffin-H00000891-M01-img0005.jpg)
![ELISA: Cyclin B1 Antibody (1C8) [H00000891-M01] - Detection limit for recombinant GST tagged CCNB1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Cyclin-B1-Antibody-1C8-ELISA-H00000891-M01-img0008.jpg)
![Western Blot: Lamin B1 Antibody (4B10) [H00004001-M01] - Analysis of LMNB1 expression in transfected 293T cell line by LMNB1 monoclonal antibody (M01), clone 4B10.Lane 1: LMNB1 transfected lysate(66.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-4B10-Western-Blot-H00004001-M01-img0009.jpg "Western Blot: Lamin B1 Antibody (4B10) [H00004001-M01] - Analysis of LMNB1 expression in transfected 293T cell line by LMNB1 monoclonal antibody (M01), clone 4B10.Lane 1: LMNB1 transfected lysate(66.4 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Lamin B1 Antibody (4B10) [H00004001-M01] - Analysis of monoclonal antibody to LMNB1 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-4B10-Immunocytochemistry-Immunofluorescence-H00004001-M01-img0003.jpg)
![Western Blot: Lamin B1 Antibody (4B10) [H00004001-M01] - LMNB1 monoclonal antibody (M01), clone 4B10. Analysis of LMNB1 expression in Raw 264.7.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-4B10-Western-Blot-H00004001-M01-img0006.jpg)
![Western Blot: Lamin B1 Antibody (4B10) [H00004001-M01] - LMNB1 monoclonal antibody (M01), clone 4B10. Analysis of LMNB1 expression in Jurkat.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-4B10-Western-Blot-H00004001-M01-img0007.jpg)
![Western Blot: Lamin B1 Antibody (4B10) [H00004001-M01] - LMNB1 monoclonal antibody (M01), clone 4B10. Analysis of LMNB1 expression in NIH/3T3.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-4B10-Western-Blot-H00004001-M01-img0008.jpg)
![Immunoprecipitation: Lamin B1 Antibody (4B10) [H00004001-M01] - Analysis of LMNB1 transfected lysate using anti-LMNB1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LMNB1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-4B10-Immunoprecipitation-H00004001-M01-img0004.jpg)
![ELISA: Lamin B1 Antibody (4B10) [H00004001-M01] - Detection limit for recombinant GST tagged LMNB1 is 3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-4B10-ELISA-H00004001-M01-img0010.jpg)
![Immunohistochemistry-Paraffin: Chk1 [p Ser296] Antibody (SN06-50) [NBP2-67711] - Analysis of paraffin-embedded human tonsil tissue using anti- phospho-Chk1 (S296) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Chk1-p-Ser296-Antibody-SN06-50-Immunohistochemistry-Paraffin-NBP2-67711-img0002.jpg "Immunohistochemistry-Paraffin: Chk1 [p Ser296] Antibody (SN06-50) [NBP2-67711] - Analysis of paraffin-embedded human tonsil tissue using anti- phospho-Chk1 (S296) antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Chk1 [p Ser296] Antibody (SN06-50) [NBP2-67711] - Analysis of paraffin-embedded human breast carcinoma tissue using anti- phospho-Chk1 (S296) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Chk1-p-Ser296-Antibody-SN06-50-Immunohistochemistry-Paraffin-NBP2-67711-img0001.jpg)
![Western Blot: RUNX1/CBFA2 Antibody (SD0803) [NBP2-67779] - Analysis of RUNX1+RUNX2+RUNX3 on Jurkat cells lysates using anti-RUNX1+RUNX2+RUNX3 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/RUNX1-CBFA2-Antibody-SD0803-Western-Blot-NBP2-67779-img0006.jpg "Western Blot: RUNX1/CBFA2 Antibody (SD0803) [NBP2-67779] - Analysis of RUNX1+RUNX2+RUNX3 on Jurkat cells lysates using anti-RUNX1+RUNX2+RUNX3 antibody at 1/1,000 dilution.")
![Immunocytochemistry/Immunofluorescence: RUNX1/CBFA2 Antibody (SD0803) [NBP2-67779] - Staining RUNX1+RUNX2+RUNX3 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/RUNX1-CBFA2-Antibody-SD0803-Immunocytochemistry-Immunofluorescence-NBP2-67779-img0007.jpg)
![Immunohistochemistry-Paraffin: RUNX1/CBFA2 Antibody (SD0803) [NBP2-67779] - Analysis of paraffin-embedded mouse testis tissue using anti-RUNX1+RUNX2+RUNX3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/RUNX1-CBFA2-Antibody-SD0803-Immunohistochemistry-Paraffin-NBP2-67779-img0005.jpg)
![Flow Cytometry: RUNX1/CBFA2 Antibody (SD0803) [NBP2-67779] - Analysis of Jurkat cells with RUNX1+RUNX2+RUNX3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/RUNX1-CBFA2-Antibody-SD0803-Flow-Cytometry-NBP2-67779-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: RUNX1/CBFA2 Antibody (SD0803) [NBP2-67779] - Staining RUNX1+RUNX2+RUNX3 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/RUNX1-CBFA2-Antibody-SD0803-Immunocytochemistry-Immunofluorescence-NBP2-67779-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: RUNX1/CBFA2 Antibody (SD0803) [NBP2-67779] - Staining RUNX1+RUNX2+RUNX3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/RUNX1-CBFA2-Antibody-SD0803-Immunocytochemistry-Immunofluorescence-NBP2-67779-img0003.jpg)
![Immunohistochemistry-Paraffin: RUNX1/CBFA2 Antibody (SD0803) [NBP2-67779] - Analysis of paraffin-embedded human tonsil tissue using anti-RUNX1+RUNX2+RUNX3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/RUNX1-CBFA2-Antibody-SD0803-Immunohistochemistry-Paraffin-NBP2-67779-img0004.jpg)
![Western Blot: BNIP3 Antibody (JA71-10) [NBP2-67192] - Analysis of BNIP3 on mouse kidney tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/BNIP3-Antibody-JA71-10-Western-Blot-NBP2-67192-img0009.jpg "Western Blot: BNIP3 Antibody (JA71-10) [NBP2-67192] - Analysis of BNIP3 on mouse kidney tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: BNIP3 Antibody (JA71-10) [NBP2-67192] - Staining BNIP3 in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/BNIP3-Antibody-JA71-10-Immunocytochemistry-Immunofluorescence-NBP2-67192-img0008.jpg)
![Immunohistochemistry-Paraffin: BNIP3 Antibody (JA71-10) [NBP2-67192] - Analysis of paraffin-embedded mouse brain tissue using anti-BNIP3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/BNIP3-Antibody-JA71-10-Immunohistochemistry-Paraffin-NBP2-67192-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: BNIP3 Antibody (JA71-10) [NBP2-67192] - Staining BNIP3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/BNIP3-Antibody-JA71-10-Immunocytochemistry-Immunofluorescence-NBP2-67192-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: BNIP3 Antibody (JA71-10) [NBP2-67192] - Staining BNIP3 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/BNIP3-Antibody-JA71-10-Immunocytochemistry-Immunofluorescence-NBP2-67192-img0007.jpg)
![Immunohistochemistry-Paraffin: BNIP3 Antibody (JA71-10) [NBP2-67192] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-BNIP3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/BNIP3-Antibody-JA71-10-Immunohistochemistry-Paraffin-NBP2-67192-img0002.jpg)
![Immunohistochemistry-Paraffin: BNIP3 Antibody (JA71-10) [NBP2-67192] - Analysis of paraffin-embedded human colon cancer tissue using anti-BNIP3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/BNIP3-Antibody-JA71-10-Immunohistochemistry-Paraffin-NBP2-67192-img0003.jpg)
![Immunohistochemistry-Paraffin: BNIP3 Antibody (JA71-10) [NBP2-67192] - Analysis of paraffin-embedded human kidney tissue using anti-BNIP3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/BNIP3-Antibody-JA71-10-Immunohistochemistry-Paraffin-NBP2-67192-img0004.jpg)
