Hu,
Mu,
Rt,
Po,
Ba,
Ch,
Ha,
I,
Rb,
YeSpecies Glossary
Reactivity: Hu, Mu, Rt, Po, Ba, Ch, Ha, I, Rb, Ye
WB, Simple Western, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
![Western Blot: GAPDH Antibody [NB300-324] - Detection of Human and Mouse GAPDH by Western Blot. Theoretical molecular weight: 36 kDa. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GAPDH antibody NB300-324 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-324-img0009.jpg "Western Blot: GAPDH Antibody [NB300-324] - Detection of Human and Mouse GAPDH by Western Blot. Theoretical molecular weight: 36 kDa. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GAPDH antibody NB300-324 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody [NB300-324] - GAPDH antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunocytochemistry-Immunofluorescence-NB300-324-img0002.jpg)
![Immunohistochemistry: GAPDH Antibody [NB300-324] - Detection of human GAPDH by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-GAPDH (NB100-79808). Detection: DAB](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunohistochemistry-NB300-324-img0010.jpg)
![Western Blot: GAPDH Antibody [NB300-324] - Detection of Human and Mouse GAPDH, theoretical molecular weight 36 kDa. Whole cell lysate from HeLa (5, 15 and 50 mcg) and mouse NIH3T3 (M; 50 mcg). Affinity purified rabbit anti-GAPDH antibody used at 0.04 mcg/ml. Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-324-img0008.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody [NB300-324] - IHC detection of GAPDH in formalin-fixed paraffin-embedded human lung carcinoma tissue sections with NB300-324 at a dilution of 1:200](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunohistochemistry-Paraffin-NB300-324-img0003.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody [NB300-324] - IHC detection of GAPDH in formalin-fixed paraffin-embedded mouse squamous cell carcinoma tissue sections with NB300-324 at a dilution of 1:200.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunohistochemistry-Paraffin-NB300-324-img0004.jpg)
![Simple Western: GAPDH Antibody [NB300-324] - Simple Western lane view shows a specific band for GAPDH in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Note: band observed higher than predicted molecular weight of 36 kDa.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Simple-Western-NB300-324-img0007.jpg)
![Western Blot: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01] - Analysis of GAPDHS expression in transfected 293T cell line by GAPDS monoclonal antibody (M01), clone 2E3-2E10.Lane 1: GAPDHS transfected lysate(44.5 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-2E3-2E10-Western-Blot-H00026330-M01-img0009.jpg "Western Blot: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01] - Analysis of GAPDHS expression in transfected 293T cell line by GAPDS monoclonal antibody (M01), clone 2E3-2E10.Lane 1: GAPDHS transfected lysate(44.5 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01] - Analysis of monoclonal antibody to GAPDHS on formalin-fixed paraffin-embedded human testis. Antibody concentration 1.5 ug/ml.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-2E3-2E10-Immunohistochemistry-Paraffin-H00026330-M01-img0007.jpg)
![Sandwich ELISA: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01] - Detection limit for recombinant GST tagged GAPDHS is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-2E3-2E10-Sandwich-ELISA-H00026330-M01-img0008.jpg)
![Western Blot: TOR/mTOR Antibody [NB100-240] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, Hep-G2, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-mTOR antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-Western-Blot-NB100-240-img0016.jpg "Western Blot: TOR/mTOR Antibody [NB100-240] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, Hep-G2, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-mTOR antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: TOR/mTOR Antibody [NB100-240] - Detection of human mTOR by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-mTOR antibody NB100-240 used for IP at 3 ug per reaction. mTOR was also immunoprecipitated by rabbit anti-mTOR antibody NB100-241. For blotting immunoprecipitated mTOR, NB100-240 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-Immunoprecipitation-NB100-240-img0015.jpg)
![Western Blot: Ribosomal Protein S6/RPS6 Antibody [NB100-1595] - Detection of human and mouse RPS6 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPS6 antibody NB100-1595 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.Previous](http://images.novusbio.com/fullsize/Ribosomal-Protein-S6-RPS6-Antibody-Western-Blot-NB100-1595-img0007.jpg "Western Blot: Ribosomal Protein S6/RPS6 Antibody [NB100-1595] - Detection of human and mouse RPS6 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPS6 antibody NB100-1595 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.Previous")
![Immunohistochemistry-Paraffin: Ribosomal Protein S6/RPS6 Antibody [NB100-1595] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti-RPS6 (NB100-1595). Detection: DAB](http://images.novusbio.com/fullsize/Ribosomal-Protein-S6-RPS6-Antibody-Immunohistochemistry-NB100-1595-img0009.jpg)
![Immunoprecipitation: Ribosomal Protein S6/RPS6 Antibody [NB100-1595] - Detection of human RPS6 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RPS6 antibody NB100-1595 (lot NB100-1595-2) used for IP at 6 ug per reaction. RPS6 was also immunoprecipitated by a previous lot of this antibody (lot NB100-1595-1). For blotting immunoprecipitated RPS6, NB100-1595 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/Ribosomal-Protein-S6-RPS6-Antibody-Immunoprecipitation-NB100-1595-img0008.jpg)
![Western Blot: Ribosomal Protein S6/RPS6 Antibody [NB100-1595] - Loss of SPAG5 & CLUH lead to hyperactivation of mTORC1 signaling.(A, B) MSigDB Hallmark pathways of downregulated (E) or upregulated (F) proteins (with a cutoff of p ≤ 0.05; q ≤ 0.15) detected in proteomics analysis of SPAG5 ind-KO cells (Figure 4A, Supplementary file 3) using the EnrichR webtool. (C) Western blots of WT & SPAG5 ind-KO HeLa cells transfected with siRNA against CLUH or untargeted control siRNA. Cells were induced for 4 days with doxycycline, additionally downregulated for the last 3 days & grown for the last 8 hr in basal or HBSS media without doxycycline. Pan-actin was used as loading control. (D) Quantification of experiments as shown in C (n = 4 independent experiments). Antibody signal was normalized to pan-actin signal, & signal of phospho-protein was normalized to signal of the total protein. Bars show mean ± standard error of the mean (SEM) & dots represent values of individual replicates. One-way analysis of variance (ANOVA) with post hoc Tukey’s multiple comparison tests were performed with *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001.Figure 5—figure supplement 1—source data 1.Uncropped blots for Figure 5—figure supplement 1C.Figure 5—figure supplement 1—source data 2.Unedited blots for Figure 5—figure supplement 1C.Uncropped blots for Figure 5—figure supplement 1C.Unedited blots for Figure 5—figure supplement 1C. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35559794), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1595_rabbit-polyclonal-ribosomal-protein-s6-rps6-antibody-31020241683913.jpg)
![Western Blot: GAPDH Antibody [NB300-326] - Detection of mouse GAPDH by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GAPDH antibody NB300-326 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-326-img0005.jpg "Western Blot: GAPDH Antibody [NB300-326] - Detection of mouse GAPDH by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GAPDH antibody NB300-326 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunohistochemistry: GAPDH Antibody [NB300-326] - Detection of mouse GAPDH by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-GAPDH (NB300-326). Detection: DAB](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunohistochemistry-NB300-326-img0004.jpg)
![Western Blot: GAPDH Antibody [NB300-326] - Detection of Mouse GAPDH Whole cell lysate (5, 15 and 50 ug) from mouse NIH3T3 cells. Affinity purified rabbit anti-GAPDH antibody used at 0.04 ug/ml. Theoretical molecular weight: 36 kDa.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-326-img0002.jpg)
![Western Blot: GAPDH Antibody [NB300-326] - Detection of GAPDH in nu/nu Mouse cells. 5% BSA in PBS, 1:5,000. Secondary: Goat anti-Rabbit IgG 1:5,000. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-326-img0003.jpg)
![Simple Western: GAPDH Antibody [NB300-326] - Simple Western lane view shows a specific band for GAPDH in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Simple-Western-NB300-326-img0001.jpg)
![Western Blot: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of eIF2A [p Ser51] on THP-1 cell lysates. Lane 1: THP-1 cells, whole cell lysate, 10ug/lane Lane 2: THP-1 cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-eIF2A [p Ser51]antibody at 1/500 dilution. Anti-GAPDH antibody at 1/10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 36 kDa Observed band size: 36 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 3 minutes 43 seconds](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Western-Blot-NBP2-67353-img0011.jpg "Western Blot: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of eIF2A [p Ser51] on THP-1 cell lysates. Lane 1: THP-1 cells, whole cell lysate, 10ug/lane Lane 2: THP-1 cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-eIF2A [p Ser51]antibody at 1/500 dilution. Anti-GAPDH antibody at 1/10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 36 kDa Observed band size: 36 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 3 minutes 43 seconds")
![Immunocytochemistry/Immunofluorescence: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Staining of Hela cells (green) using NBP2-67353. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunocytochemistry-Immunofluorescence-NBP2-67353-img0008.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-elF2A [p Ser51] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0013.jpg)
![Flow Cytometry: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of Hela cells with Phospho-eIF-2a(S51) antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Flow-Cytometry-NBP2-67353-img0001.jpg)
![Western Blot: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of Phospho-eIF-2a(S51) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: HUVEC cell lysateLane 2: Hela cell lysateLane 2: PC-3M cell lysate](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Western-Blot-NBP2-67353-img0009.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded human liver tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0002.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded human pancreas tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0003.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded mouse brain tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0004.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded mouse pancreas tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0005.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded mouse placenta tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0006.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue using anti-eIF2A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-14, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0010.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded mouse brain tissue with Rabbit anti-eIF2A [p Ser51] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/eIF2A-p-Ser51-Antibody-SZ01-06-Immunohistochemistry-Paraffin-NBP2-67353-img0012.jpg)
![Western Blot: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of BL-21 bacteria (1), Sf9 insect (2), and Saccharomyces cerevisiae (3). Theoretical molecular weight: 36 kDa.](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Western-Blot-NBP2-37828-img0019.jpg "Western Blot: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of BL-21 bacteria (1), Sf9 insect (2), and Saccharomyces cerevisiae (3). Theoretical molecular weight: 36 kDa.")
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of GAPDH (green) in HeLa. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 5% normal goat serum for 15 minutes at room temperature. Cells were probed with a GAPDH monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Immunocytochemistry-Immunofluorescence-NBP2-37828-img0012.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis showing staining in the nucleus and cytoplasm of paraffin-embedded human breast carcinoma (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Immunohistochemistry-Paraffin-NBP2-37828-img0015.jpg)
![Flow Cytometry: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of GAPDH in Hela cells (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Flow-Cytometry-NBP2-37828-img0008.jpg)
![Western Blot: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of 5ug/lane tissue lysates from human (1), mouse (2), rat (3), rabbit (4), chicken (5), and hamster (6). Theoretical molecular weight: 36 kDa.](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Western-Blot-NBP2-37828-img0018.jpg)
![Western Blot: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of 20ug of THP-1 whole cell lysates per well onto an SDS-PAGE gel. GAPDH theoretical molecular weight: 36 kDa.](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Western-Blot-NBP2-37828-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of GAPDH (green) showing staining in the in the cytoplasm of MCF-7 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Immunocytochemistry-Immunofluorescence-NBP2-37828-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of GAPDH (green) showing staining in the in the cytoplasm of Hela cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Immunocytochemistry-Immunofluorescence-NBP2-37828-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of GAPDH (green) showing staining in the in the cytoplasm of C2C12 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Immunocytochemistry-Immunofluorescence-NBP2-37828-img0011.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis showing staining in the nucleus and cytoplasm of mouse colon tissue (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Immunohistochemistry-Paraffin-NBP2-37828-img0013.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis showing staining in the nucleus and cytoplasm of human kidney tissue (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Immunohistochemistry-Paraffin-NBP2-37828-img0014.jpg)
![Flow Cytometry: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of GAPDH in NIH-3T3 cells (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Flow-Cytometry-NBP2-37828-img0006.jpg)
![Flow Cytometry: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of GAPDH in MCF-7 cells (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/GAPDH-Antibody-GA1R-Flow-Cytometry-NBP2-37828-img0007.jpg)
![Western Blot: PUM2 Antibody [NB100-387] - Functionality of products expressed from miRNA134. 1, 2 and miRNA99a.1. miR-134 expressed from the miRNA134.1 construct affects the Pum2 protein levels. 10-11 DIV hippocampal neurons were transduced with purified rAAV carrying the indicated plasmids. Eight days later whole cell extracts were prepared and subjected to western blot analysis probing with Pum2 antibody and a beta-actin antibody as loading control. Here we show one out of three representative blots, on average displaying a significant 64% reduction in Pum2 protein levels. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/neuro.04.016.2009/abstract), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PUM2-Antibody-Western-Blot-NB100-387-img0003.jpg "Western Blot: PUM2 Antibody [NB100-387] - Functionality of products expressed from miRNA134. 1, 2 and miRNA99a.1. miR-134 expressed from the miRNA134.1 construct affects the Pum2 protein levels. 10-11 DIV hippocampal neurons were transduced with purified rAAV carrying the indicated plasmids. Eight days later whole cell extracts were prepared and subjected to western blot analysis probing with Pum2 antibody and a beta-actin antibody as loading control. Here we show one out of three representative blots, on average displaying a significant 64% reduction in Pum2 protein levels. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/neuro.04.016.2009/abstract), licensed under a CC-BY license.")
![Western Blot: PUM2 Antibody [NB100-387] - Detection of Human and Mouse Pumilio 2 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Pumilio 2 antibody NB100-387 used for WB at 0.2 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate.](http://images.novusbio.com/fullsize/PUM2-Antibody-Western-Blot-NB100-387-img0002.jpg)
![Western Blot: YB1 Antibody (9J7M4) [NBP3-16213] - Analysis of extracts of various cell lines, using YB1 Rabbit mAb (NBP3-16213) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/YB1-Antibody-9J7M4-Western-Blot-NBP3-16213-img0008.jpg "Western Blot: YB1 Antibody (9J7M4) [NBP3-16213] - Analysis of extracts of various cell lines, using YB1 Rabbit mAb (NBP3-16213) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: YB1 Antibody (9J7M4) [NBP3-16213] - Analysis of extracts of HeLa cells, using YB1 Rabbit mAb (NBP3-16213) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/YB1-Antibody-9J7M4-Western-Blot-NBP3-16213-img0007.jpg)
![Immunoprecipitation: YB1 Antibody (9J7M4) [NBP3-16213] - Immunoprecipitation of YB1 from 200 ug extracts of HeLa cells was performed using 0.5 ug of YB1 Rabbit mAb . Rabbit IgG isotype control was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using YB1 Rabbit mAb at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-16213_rabbit-yb1-mab-9j7m4-5220251255573.jpg)
![Immunocytochemistry/ Immunofluorescence: YB1 Antibody (9J7M4) [NBP3-16213] - Confocal imaging of NIH/3T3 cells using YB1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16213_rabbit-yb1-mab-9j7m4-52202511361248.jpg)
![Immunohistochemistry: YB1 Antibody (9J7M4) [NBP3-16213] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using YB1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16213_rabbit-yb1-mab-9j7m4-5220251215454.jpg)
![Immunohistochemistry: YB1 Antibody (9J7M4) [NBP3-16213] - Immunohistochemistry analysis of paraffin-embedded Human colon using YB1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16213_rabbit-yb1-mab-9j7m4-52202511374130.jpg)
![Immunohistochemistry: YB1 Antibody (9J7M4) [NBP3-16213] - Immunohistochemistry analysis of paraffin-embedded Rat kidney using YB1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16213_rabbit-yb1-mab-9j7m4-52202511411489.jpg)
![Immunohistochemistry: YB1 Antibody (9J7M4) [NBP3-16213] - Immunohistochemistry analysis of paraffin-embedded Rat colon using YB1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16213_rabbit-yb1-mab-9j7m4-5220251140277.jpg)
![Immunohistochemistry: YB1 Antibody (9J7M4) [NBP3-16213] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using YB1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16213_rabbit-yb1-mab-9j7m4-52202511392221.jpg)
![Immunocytochemistry/ Immunofluorescence: YB1 Antibody (9J7M4) [NBP3-16213] - Confocal imaging of U-2 OS cells using YB1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16213_rabbit-yb1-mab-9j7m4-52202511374198.jpg)
![Immunocytochemistry/ Immunofluorescence: YB1 Antibody (9J7M4) [NBP3-16213] - Confocal imaging of C6 cells using YB1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16213_rabbit-yb1-mab-9j7m4-52202511402797.jpg)
![Western Blot: hnRNP H Antibody [NBP1-31648] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP H antibody diluted by 1:1000.](http://images.novusbio.com/fullsize/hnRNP-H-Antibody-Western-Blot-NBP1-31648-img0008.jpg "Western Blot: hnRNP H Antibody [NBP1-31648] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP H antibody diluted by 1:1000.")
![Immunocytochemistry/Immunofluorescence: hnRNP H Antibody [NBP1-31648] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: hnRNP H protein stained by hnRNP H antibody [N1C1] diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/hnRNP-H-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31648-img0013.jpg)
![Immunohistochemistry-Paraffin: hnRNP H Antibody [NBP1-31648] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/hnRNP-H-Antibody-Immunohistochemistry-Paraffin-NBP1-31648-img0005.jpg)
![Western Blot: hnRNP H Antibody [NBP1-31648] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/hnRNP-H-Antibody-Western-Blot-NBP1-31648-img0006.jpg)
![Western Blot: hnRNP H Antibody [NBP1-31648] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 C: Hela D: Hep G2 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/hnRNP-H-Antibody-Western-Blot-NBP1-31648-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: hnRNP H Antibody [NBP1-31648] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: hnRNP H protein stained by hnRNP H antibody [N1C1] diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] (NBP2-43837) diluted at 1:1000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/hnRNP-H-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31648-img0011.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: hnRNP H Antibody [NBP1-31648] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP H antibody [N1C1] diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31648_rabbit-polyclonal-hnrnp-h-antibody-1610202419405727.jpg)
![Western Blot: eIF2A [p Ser51] Antibody [NB100-81896] - Analysis of extracts from Jurkat cells untreated or treated with H2O2 using eIF2 alpha (Phospho-Ser51) antibody.](http://images.novusbio.com/fullsize/eIF2A-[p-Ser51]-Antibody-Western-Blot-NB100-81896-img0009.jpg "<b>Biological Strategies Validation. </b>Western Blot: eIF2A [p Ser51] Antibody [NB100-81896] - Analysis of extracts from Jurkat cells untreated or treated with H2O2 using eIF2 alpha (Phospho-Ser51) antibody.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody [NB100-81896] - Paraffin-embedded human lung carcinoma tissue.](http://images.novusbio.com/fullsize/eIF2A-[p-Ser51]-Antibody-Immunohistochemistry-Paraffin-NB100-81896-img0011.jpg)
![Western Blot: eIF2A [p Ser51] Antibody [NB100-81896] - Western blot analysis of lysates from 1) KB, 2) MCF-7 cells, (Green) primary antibody was diluted at 1: 1000,](http://images.novusbio.com/fullsize/nb100-81896_rabbit-polyclonal-eif2a-p-ser51-antibody-23420241523258.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody [NB100-81896] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NB100-81896(left) or the same antibody preincubated with blocking peptide (right).](http://images.novusbio.com/fullsize/nb100-81896_rabbit-polyclonal-eif2a-p-ser51-antibody-103202523464519.jpg)
![Immunocytochemistry/Immunofluorescence: eIF2A [p Ser51] Antibody [NB100-81896] - Staining of methanol-fixed HeLa cells using NB100-81896](http://images.novusbio.com/fullsize/nb100-81896_rabbit-polyclonal-eif2a-p-ser51-antibody-1132025155593.jpg)
![Western Blot: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25721_rabbit-thymidylate-synthase-mab-hl1236-azide-bsa-free-2120249374982.jpg "Western Blot: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25721_rabbit-thymidylate-synthase-mab-hl1236-azide-bsa-free-2120249383186.jpg)
![Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Thymidylate synthase antibody [HL1236] detects Thymidylate synthase protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded dog tissue. Thymidylate synthase stained by Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25721_rabbit-thymidylate-synthase-mab-hl1236-azide-bsa-free-2120249355536.jpg)
![Western Blot: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25721_rabbit-thymidylate-synthase-mab-hl1236-azide-bsa-free-2120249403876.jpg)
![Immunocytochemistry/Immunofluorescence: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Thymidylate synthase antibody [HL1236] detects Thymidylate synthase protein at cytoplasm and nucleus and mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Thymidylate synthase stained by Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25721_rabbit-thymidylate-synthase-mab-hl1236-azide-bsa-free-212024937157.jpg)
![Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Thymidylate synthase antibody [HL1236] detects Thymidylate synthase protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded cat brain. Thymidylate synthase stained by Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25721_rabbit-thymidylate-synthase-mab-hl1236-azide-bsa-free-2120249415929.jpg)
![Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Thymidylate synthase antibody [HL1236] detects Thymidylate synthase protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded dog brain. Thymidylate synthase stained by Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25721_rabbit-thymidylate-synthase-mab-hl1236-azide-bsa-free-212024941337.jpg)