| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26233-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34620-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33056 | |||
| IHC-Prediluted | NBP2-44327 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-59628 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48410 | |||
| Azide and BSA Free | NBP3-11546 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44977 | |||
| Azide and BSA Free | NBP2-34817-0.1mg | |||
![Immunohistochemistry: FIH-1/HIF-1AN Antibody (12F11) [NBP3-26233] - Image of FIH-1/HIF-1AN Antibody (12F11) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26233_rabbit-fih-1-hif-1an-mab-12f11-262202415355734.jpg "Immunohistochemistry: FIH-1/HIF-1AN Antibody (12F11) [NBP3-26233] - Image of FIH-1/HIF-1AN Antibody (12F11) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: FIH-1/HIF-1AN Antibody (12F11) [NBP3-26233] - Image of FIH-1/HIF-1AN Antibody (12F11) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26233_rabbit-fih-1-hif-1an-mab-12f11-272202485636.jpg)
![Western Blot: FIH-1/HIF-1AN Antibody (12F11) [NBP3-26233] - Positive Western Blot detected in: Ntera-2 whole cell lysate, Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, PC3 whole cell lysate, U87 whole cell lysate. All lanes: FIH-1/HIF-1AN Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 41 kDa Observed band size: 36-53 kDa](http://images.novusbio.com/fullsize/nbp3-26233_rabbit-fih-1-hif-1an-mab-12f11-282202411442647.jpg)
![Immunocytochemistry/Immunofluorescence: FIH-1/HIF-1AN Antibody (12F11) [NBP3-26233] - Staining of HepG2 cell with FIH-1/HIF-1AN Antibody (12F11) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 525-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26233_rabbit-fih-1-hif-1an-mab-12f11-28220241312243.jpg)
![Flow Cytometry: FIH-1/HIF-1AN Antibody (12F11) [NBP3-26233] - Overlay Peak curve showing Hela cells stained with FIH-1/HIF-1AN Antibody (12F11) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26233_rabbit-fih-1-hif-1an-mab-12f11-282202414312744.jpg)
![Immunohistochemistry-Paraffin: FoxP3 Antibody (FXP3/197) [NBP2-34282] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with FOXP3 Monoclonal Antibody (FXP3/197).](http://images.novusbio.com/fullsize/FoxP3-Antibody-FXP3-197-Immunohistochemistry-Paraffin-NBP2-34282-img0002.jpg "Immunohistochemistry-Paraffin: FoxP3 Antibody (FXP3/197) [NBP2-34282] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with FOXP3 Monoclonal Antibody (FXP3/197).")
![Immunohistochemistry-Paraffin: FoxP3 Antibody (FXP3/197) [NBP2-34282] - Formalin-fixed, paraffin-embedded human Tonsil stained with FOXP3 Monoclonal Antibody (FXP3/197).](http://images.novusbio.com/fullsize/FoxP3-Antibody-FXP3-197-Immunohistochemistry-Paraffin-NBP2-34282-img0001.jpg)
![Immunohistochemistry-Paraffin: Myogenin Antibody (MGN185) [NBP2-29431] - Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with MyoD Antibody (MYOD1/2075R).](http://images.novusbio.com/fullsize/Myogenin-Antibody-MGN185-Immunohistochemistry-Paraffin-NBP2-29431-img0003.jpg "Immunohistochemistry-Paraffin: Myogenin Antibody (MGN185) [NBP2-29431] - Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with MyoD Antibody (MYOD1/2075R).")
![Western Blot: p53 Antibody (TRP/816) [NBP2-59626] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-59626) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/p53-Antibody-TRP-816-Knockout-Validated-NBP2-59626-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody (TRP/816) [NBP2-59626] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-59626) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Protein Array: p53 Antibody (TRP/816) [NBP2-59626] - containing more than 19,000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary) produces when binding to a particular protein on the HuProt array. Z-scores are described in units of standard deviations above the mean value of all signals generated on that array. If targets on HuProt are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of an Ab to its intended target. An Ab is considered to specific to its intended target, if the Ab has an S-score of at least 2.5. For example, if Ab binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Ab to protein X is equal to 29](http://images.novusbio.com/fullsize/p53-Antibody-TRP-816-Protein-Array-NBP2-59626-img0001.jpg)
![Western Blot: p53 Antibody (TRP/816) [NBP2-59626] - Western Blot Analysis of human HeLa cell lysate using p53 Antibody (TRP/816).](http://images.novusbio.com/fullsize/p53-Antibody-TRP-816-Western-Blot-NBP2-59626-img0006.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (TRP/816) [NBP2-59626] - Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with p53 Mouse Monoclonal Antibody (TRP/816).](http://images.novusbio.com/fullsize/p53-Antibody-TRP-816-Immunohistochemistry-Paraffin-NBP2-59626-img0002.jpg)
![Western Blot: PIMT Antibody [NB100-2595] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NB100-2595 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B).](http://images.novusbio.com/fullsize/PIMT-Antibody-Western-Blot-NB100-2595-img0004.jpg "Western Blot: PIMT Antibody [NB100-2595] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NB100-2595 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B).")
![Immunohistochemistry: PIMT Antibody [NB100-2595] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PIMT used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PIMT-Antibody-Immunohistochemistry-NB100-2595-img0005.jpg)
![Immunohistochemistry-Paraffin: PIMT Antibody [NB100-2595] - FFPE section of human non-small cell lung cancer. Affinity purified rabbit anti-PIMT used at a dilution of 1:250.](http://images.novusbio.com/fullsize/PIMT-Antibody-Immunohistochemistry-Paraffin-NB100-2595-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: PIMT Antibody [NB100-2595] - Detection of human PIMT by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PIMT antibody NB100-2595 (lot NB100-2595-2) used for IP at 6 ug per reaction. PIMT was also immunoprecipitated by a previous lot of this antibody (lot NB100-2595-1) and rabbit anti-PIMT antibody NBP2-44294. For blotting immunoprecipitated PIMT, NB100-2595 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/PIMT-Antibody-Immunoprecipitation-NB100-2595-img0006.jpg)
![Western Blot: ASH2L Antibody [NB600-281] - Detection of Human ASH2on HeLa whole cell lysate using NB600-281. ASH2 was also immunoprecipitated with rabbit anti-ASH2 antibody NB600-250.](http://images.novusbio.com/fullsize/ASH2L-Antibody-Western-Blot-NB600-281-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: ASH2L Antibody [NB600-281] - Detection of Human ASH2on HeLa whole cell lysate using NB600-281. ASH2 was also immunoprecipitated with rabbit anti-ASH2 antibody NB600-250.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ASH2L Antibody [NB600-281] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- ASH2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/ASH2L-Antibody-Immunohistochemistry-Paraffin-NB600-281-img0013.jpg)
![Chromatin Immunoprecipitation: ASH2L Antibody [NB600-281] - ChIP-chip scatter plot of anti-Ash2 enriched DNA binding sites versus input reference DNA. A. 10 ug of NB600-281 was used to immunoprecipitate chromatin from K562 cells according to Ren et al (Genes Dev. 2002 16: 245-256). Immunoprecipitated DNA and reference DNA were amplified via ligation-mediated PCR and the products abeled with fluorescent dNTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites. B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-Ash2 ChIP, normal rabbit IgG showed little enrichment.](http://images.novusbio.com/fullsize/ASH2L-Antibody-Chromatin-Immunoprecipitation-NB600-281-img0009.jpg)
![Western Blot: p53 Antibody (TRP/817) [NBP2-44982] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-44982) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/p53-Antibody-TRP-817-Knockout-Validated-NBP2-44982-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody (TRP/817) [NBP2-44982] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-44982) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody (TRP/817) [NBP2-44982] - Western Blot Analysis of HeLa cell lysate using p53 Antibody (TRP/817).](http://images.novusbio.com/fullsize/p53-Antibody-TRP-817-Western-Blot-NBP2-44982-img0005.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (TRP/817) [NBP2-44982] - Human Colon Carcinoma stained with p53 Monoclonal Antibody (TRP/817)](http://images.novusbio.com/fullsize/p53-Antibody-TRP-817-Immunohistochemistry-Paraffin-NBP2-44982-img0001.jpg)
![Western Blot: ZBTB7A/Pokemon Antibody [NB100-762] - Detection of Human ZBTB7(FBI-1) on HeLa whole cell lysate using NB100-762. ZBTB7(FBI-1) was also immunoprecipitated using rabbit antibodies NB100-761 and another ZBTB7(FBI-1) Ab.](http://images.novusbio.com/fullsize/ZBTB7A-Pokemon-Antibody-Western-Blot-NB100-762-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: ZBTB7A/Pokemon Antibody [NB100-762] - Detection of Human ZBTB7(FBI-1) on HeLa whole cell lysate using NB100-762. ZBTB7(FBI-1) was also immunoprecipitated using rabbit antibodies NB100-761 and another ZBTB7(FBI-1) Ab.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: ZBTB7A/Pokemon Antibody [NB100-762] - Section of human colon carcinoma. Antibody: Affinity purified rabbit anti- ZBTB7/FBI-1 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/ZBTB7A-Pokemon-Antibody-Immunohistochemistry-NB100-762-img0003.jpg)
![Western Blot: Nab2 Antibody [NBP2-19462] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:2000.](http://images.novusbio.com/fullsize/Nab2-Antibody-Western-Blot-NBP2-19462-img0001.jpg "Western Blot: Nab2 Antibody [NBP2-19462] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:2000.")
![Immunohistochemistry-Paraffin: Nab2 Antibody [NBP2-19462] - Mouse fore brain. NAB2 antibody dilution: 1:500. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min](http://images.novusbio.com/fullsize/Nab2-Antibody-Immunohistochemistry-Paraffin-NBP2-19462-img0003.jpg)
![Immunoprecipitation: Nab2 Antibody [NBP2-19462] - Protein from Jurkat whole cell extracts using 5 ug of NAB2 antibody. Western blot analysis was performed using NAB2 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Nab2-Antibody-Immunoprecipitation-NBP2-19462-img0005.jpg)
![Western Blot: p53 Antibody (SPM589) [NBP2-32891] - Western Blot Analysis of HeLa cell lysate using p53 Antibody (SPM589)](http://images.novusbio.com/fullsize/p53-Antibody-SPM589-Western-Blot-NBP2-32891-img0004.jpg "Western Blot: p53 Antibody (SPM589) [NBP2-32891] - Western Blot Analysis of HeLa cell lysate using p53 Antibody (SPM589)")
![Immunohistochemistry-Paraffin: p53 Antibody (SPM589) [NBP2-32891] - Formalin-fixed paraffin-embedded human bladder carcinoma stained with p53 monoclonal antibody (SPM589)](http://images.novusbio.com/fullsize/p53-Antibody-SPM589-Immunohistochemistry-Paraffin-NBP2-32891-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: E2F-1 Antibody (S03-5G3) [NBP3-19622] - Immunofluorescence of E2F-1(green) in Hela cells using NBP3-19622 at dilution 1/50, and DAPI(blue)](http://images.novusbio.com/fullsize/nbp3-19622_rabbit-e2f-1-mab-s03-5g3-103202523464528.jpg "Immunocytochemistry/ Immunofluorescence: E2F-1 Antibody (S03-5G3) [NBP3-19622] - Immunofluorescence of E2F-1(green) in Hela cells using NBP3-19622 at dilution 1/50, and DAPI(blue)")
![Western Blot: E2F-1 Antibody (S03-5G3) [NBP3-19622] - Western blot detection of E2F-1 in C6, 3T3 cell lysates using NBP3-19622. Predicted band size: 47kDa. Observed band size: 70kDa.](http://images.novusbio.com/fullsize/nbp3-19622_rabbit-e2f-1-mab-s03-5g3-103202523472632.jpg)
![Immunohistochemistry-Paraffin: E2F-1 Antibody (S03-5G3) [NBP3-19622] - Immunohistochemistry of E2F-1 in paraffin-embedded Human tonsil using NBP3-19622 at dilution 1/2](http://images.novusbio.com/fullsize/nbp3-19622_rabbit-e2f-1-mab-s03-5g3-10320252332109.jpg)
![Western Blot: Pax5/BSAP Antibody (8F9) [H00005079-M01] - Analysis of PAX5 expression in transfected 293T cell line by PAX5 monoclonal antibody (M01), clone 8F9.Lane 1: PAX5 transfected lysate(42.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Pax5-BSAP-Antibody-8F9-Western-Blot-H00005079-M01-img0011.jpg "Western Blot: Pax5/BSAP Antibody (8F9) [H00005079-M01] - Analysis of PAX5 expression in transfected 293T cell line by PAX5 monoclonal antibody (M01), clone 8F9.Lane 1: PAX5 transfected lysate(42.1 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: Pax5/BSAP Antibody (8F9) [H00005079-M01] - Analysis of monoclonal antibody to PAX5 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 1.5 ug/ml.](http://images.novusbio.com/fullsize/Pax5-BSAP-Antibody-8F9-Immunohistochemistry-Paraffin-H00005079-M01-img0007.jpg)
![Western Blot: Pax5/BSAP Antibody (8F9) [H00005079-M01] - Analysis of PAX5 over-expressed 293 cell line, cotransfected with PAX5 Validated Chimera RNAi ( Cat # H00005079-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PAX5 monoclonal antibody (M01), clone 8F9 (Cat # H00005079-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/Pax5-BSAP-Antibody-8F9-Western-Blot-H00005079-M01-img0008.jpg)
![Western Blot: Pax5/BSAP Antibody (8F9) [H00005079-M01] - PAX5 monoclonal antibody (M01), clone 8F9. Analysis of PAX5 expression in IMR-32.](http://images.novusbio.com/fullsize/Pax5-BSAP-Antibody-8F9-Western-Blot-H00005079-M01-img0010.jpg)
![ELISA: Pax5/BSAP Antibody (8F9) [H00005079-M01] - Detection limit for recombinant GST tagged PAX5 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Pax5-BSAP-Antibody-8F9-ELISA-H00005079-M01-img0009.jpg)