| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86960-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87338-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44027 | |||
| BSA Free | NBP2-52911-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18571-25ul | |||
| Azide and BSA Free | NBP3-44392 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43991 | |||
| BSA Free | NBP2-34489-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89842-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18552-25ul | |||
| Azide and BSA Free | NBP3-44391 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92028-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43926 | |||
| BSA Free | NBP2-34478-25ul | |||
![Western Blot: LEF1 Antibody [NBP1-86960] - Analysis in human cell lines SK-MEL-30 and A-549 using anti-LEF1 antibody. Corresponding LEF1 RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.](http://images.novusbio.com/fullsize/LEF1-Antibody-Western-Blot-NBP1-86960-img0023.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: LEF1 Antibody [NBP1-86960] - Analysis in human cell lines SK-MEL-30 and A-549 using anti-LEF1 antibody. Corresponding LEF1 RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: LEF1 Antibody [NBP1-86960] - Analysis in control (vector only transfected HEK293T lysate) and LEF1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/LEF1-Antibody-Western-Blot-NBP1-86960-img0025.jpg)
![Immunocytochemistry/Immunofluorescence: LEF1 Antibody [NBP1-86960] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/LEF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86960-img0024.jpg)
![Immunohistochemistry-Paraffin: LEF1 Antibody [NBP1-86960] - Staining of human oral mucosa shows moderate nuclear positivity in subset of squamous epithelial cells.](http://images.novusbio.com/fullsize/LEF1-Antibody-Immunohistochemistry-Paraffin-NBP1-86960-img0018.jpg)
![Immunohistochemistry-Paraffin: LEF1 Antibody [NBP1-86960] - Staining of human lymph node shows moderate to strong nuclear positivity in non - germinal center cells.](http://images.novusbio.com/fullsize/LEF1-Antibody-Immunohistochemistry-Paraffin-NBP1-86960-img0017.jpg)
![Western Blot: PAWR / PAR4 Antibody [NBP1-87338] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/PAWR-PAR4-Antibody-Western-Blot-NBP1-87338-img0017.jpg "Western Blot: PAWR / PAR4 Antibody [NBP1-87338] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunocytochemistry/Immunofluorescence: PAWR / PAR4 Antibody [NBP1-87338] - Staining of human cell line SiHa shows localization to plasma membrane & actin filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PAWR---PAR4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87338-img0008.jpg)
![Immunohistochemistry-Paraffin: PAWR / PAR4 Antibody [NBP1-87338] - Staining of human skeletal muscle shows no positivity as expected.](http://images.novusbio.com/fullsize/PAWR---PAR4-Antibody-Immunohistochemistry-Paraffin-NBP1-87338-img0015.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: PAWR / PAR4 Antibody [NBP1-87338] - Analysis in human cell lines Caco-2 and SK-MEL-30. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HDAC1.](http://images.novusbio.com/fullsize/PAWR---PAR4-Antibody-Western-Blot-NBP1-87338-img0010.jpg)
![Immunohistochemistry-Paraffin: PAWR / PAR4 Antibody [NBP1-87338] - Staining of human endometrium shows strong cytoplasmic positivity in glandular cells and smooth muscle cells.](http://images.novusbio.com/fullsize/PAWR---PAR4-Antibody-Immunohistochemistry-Paraffin-NBP1-87338-img0012.jpg)
![Immunohistochemistry-Paraffin: PAWR / PAR4 Antibody [NBP1-87338] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PAWR---PAR4-Antibody-Immunohistochemistry-Paraffin-NBP1-87338-img0013.jpg)
![Immunohistochemistry-Paraffin: PAWR / PAR4 Antibody [NBP1-87338] - Staining of human cerebellum shows moderate to strong cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/PAWR---PAR4-Antibody-Immunohistochemistry-Paraffin-NBP1-87338-img0014.jpg)
![Western Blot: CTCF Antibody (CL0307) [NBP2-52911] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0307-Western-Blot-NBP2-52911-img0007.jpg "Western Blot: CTCF Antibody (CL0307) [NBP2-52911] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG")
![Immunocytochemistry/Immunofluorescence: CTCF Antibody (CL0307) [NBP2-52911] - Staining of MCF7 cells using the Anti-CTCF monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0307-Immunocytochemistry-Immunofluorescence-NBP2-52911-img0012.jpg)
![Immunohistochemistry-Paraffin: CTCF Antibody (CL0307) [NBP2-52911] - Analysis of CTCF antibody on Human fallopian tube tissue. Antigen retrieval was done enzymatically. Image from verified customer review.](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0307-Immunohistochemistry-Paraffin-NBP2-52911-img0013.jpg)
![Immunohistochemistry-Paraffin: CTCF Antibody (CL0307) [NBP2-52911] - Staining of human endometrium shows strong nuclear positivity in glandular and stromal cells](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0307-Immunohistochemistry-Paraffin-NBP2-52911-img0008.jpg)
![Immunohistochemistry-Paraffin: CTCF Antibody (CL0307) [NBP2-52911] - Staining of human kidney shows strong nuclear positivity in cells in glomeruli and tubuli.](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0307-Immunohistochemistry-Paraffin-NBP2-52911-img0009.jpg)
![Immunohistochemistry-Paraffin: CTCF Antibody (CL0307) [NBP2-52911] - Staining of human small intestine shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0307-Immunohistochemistry-Paraffin-NBP2-52911-img0010.jpg)
![Immunohistochemistry-Paraffin: CTCF Antibody (CL0307) [NBP2-52911] - Staining of human testis shows moderate nuclear positivity in a subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0307-Immunohistochemistry-Paraffin-NBP2-52911-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: CDX2 Antibody (CL12967) [NBP3-18571] - Analysis in human cell line CACO-2 and human cell line PC-3.](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12967-Immunocytochemistry-Immunofluorescence-NBP3-18571-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CDX2 Antibody (CL12967) [NBP3-18571] - Analysis in human cell line CACO-2 and human cell line PC-3.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12967-Immunohistochemistry-Paraffin-NBP3-18571-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CDX2 Antibody (CL12967) [NBP3-18571] - Staining of CACO-2 cells using the Anti-CDX2 monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule probes are visualized in red (where available).](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12967-Immunocytochemistry-Immunofluorescence-NBP3-18571-img0001.jpg)
![Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571] - Analysis in human colon and tonsil tissues using NBP3-18571 antibody. Corresponding CDX2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12967-Immunohistochemistry-Paraffin-NBP3-18571-img0002.jpg)
![Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571] - Staining of human colorectal cancer shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12967-Immunohistochemistry-Paraffin-NBP3-18571-img0003.jpg)
![Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571] - Staining of human colorectal cancer (metastasis to ovary) shows moderate to strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12967-Immunohistochemistry-Paraffin-NBP3-18571-img0004.jpg)
![Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571] - Staining of human colon shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12967-Immunohistochemistry-Paraffin-NBP3-18571-img0005.jpg)
![Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571] - Staining of human rectum shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12967-Immunohistochemistry-Paraffin-NBP3-18571-img0006.jpg)
![Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12967-Immunohistochemistry-Paraffin-NBP3-18571-img0007.jpg)
![Immunohistochemistry-Paraffin: ATF3 Antibody (CL1685) [NBP2-34489] - Staining in human placenta and tonsil tissues. Corresponding ATF3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ATF3-Antibody-CL1685-Immunohistochemistry-Paraffin-NBP2-34489-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ATF3 Antibody (CL1685) [NBP2-34489] - Staining in human placenta and tonsil tissues. Corresponding ATF3 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ATF3 Antibody (CL1685) [NBP2-34489] - Staining of RH-30 cells using the Anti-ATF3 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ATF3-Antibody-CL1685-Immunocytochemistry-Immunofluorescence-NBP2-34489-img0013.jpg)
![Immunohistochemistry-Paraffin: ATF3 Antibody (CL1685) [NBP2-34489] - Staining of human stomach cancer shows moderate to strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/ATF3-Antibody-CL1685-Immunohistochemistry-Paraffin-NBP2-34489-img0012.jpg)
![Immunohistochemistry-Paraffin: ATF3 Antibody (CL1685) [NBP2-34489] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/ATF3-Antibody-CL1685-Immunohistochemistry-Paraffin-NBP2-34489-img0007.jpg)
![Immunohistochemistry-Paraffin: ATF3 Antibody (CL1685) [NBP2-34489] - Staining of human fallopian tube shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/ATF3-Antibody-CL1685-Immunohistochemistry-Paraffin-NBP2-34489-img0008.jpg)
![Immunohistochemistry-Paraffin: ATF3 Antibody (CL1685) [NBP2-34489] - Staining of human tonsil shows no positivity in lymphoid cells as expected.](http://images.novusbio.com/fullsize/ATF3-Antibody-CL1685-Immunohistochemistry-Paraffin-NBP2-34489-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: FRA2 Antibody [NBP1-89842] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/FRA2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89842-img0010.jpg "Immunocytochemistry/Immunofluorescence: FRA2 Antibody [NBP1-89842] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: FRA2 Antibody [NBP1-89842] - Staining of human stomach shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/FRA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89842-img0019.jpg)
![Immunohistochemistry-Paraffin: FRA2 Antibody [NBP1-89842] - Staining of human cervix shows moderate to strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/FRA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89842-img0016.jpg)
![Immunohistochemistry-Paraffin: FRA2 Antibody [NBP1-89842] - Staining of human skin shows moderate to strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/FRA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89842-img0018.jpg)
![Western Blot: FRA2 Antibody [NBP1-89842] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/FRA2-Antibody-Western-Blot-NBP1-89842-img0007.jpg)
![Western Blot: CDX2 Antibody (CL12974) [NBP3-18552] - Analysis in human cell line CACO-2 and human cell line PC-3.](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12974-Western-Blot-NBP3-18552-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: CDX2 Antibody (CL12974) [NBP3-18552] - Analysis in human cell line CACO-2 and human cell line PC-3.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CDX2 Antibody (CL12974) [NBP3-18552] - Staining of CACO-2 cells using the Anti-CDX2 monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule is visualized in red (where available).](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12974-Immunocytochemistry-Immunofluorescence-NBP3-18552-img0001.jpg)
![Immunohistochemistry-Paraffin: CDX2 Antibody (CL12974) [NBP3-18552] - Staining of human lymph node shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12974-Immunohistochemistry-Paraffin-NBP3-18552-img0007.jpg)
![Immunohistochemistry-Paraffin: CDX2 Antibody (CL12974) [NBP3-18552] - Analysis in human rectum and lymph node tissues using NBP3-18552 antibody. Corresponding CDX2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12974-Immunohistochemistry-Paraffin-NBP3-18552-img0002.jpg)
![Immunohistochemistry-Paraffin: CDX2 Antibody (CL12974) [NBP3-18552] - Staining of human colorectal cancer shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12974-Immunohistochemistry-Paraffin-NBP3-18552-img0003.jpg)
![Immunohistochemistry-Paraffin: CDX2 Antibody (CL12974) [NBP3-18552] - Staining of human colorectal cancer (metastasis to ovary) shows moderate to strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12974-Immunohistochemistry-Paraffin-NBP3-18552-img0004.jpg)
![Immunohistochemistry-Paraffin: CDX2 Antibody (CL12974) [NBP3-18552] - Staining of human rectum shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12974-Immunohistochemistry-Paraffin-NBP3-18552-img0005.jpg)
![Immunohistochemistry-Paraffin: CDX2 Antibody (CL12974) [NBP3-18552] - Staining of human colon shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/CDX2-Antibody-CL12974-Immunohistochemistry-Paraffin-NBP3-18552-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: IRF3 Antibody [NBP1-92028] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.](http://images.novusbio.com/fullsize/IRF3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92028-img0008.jpg "Immunocytochemistry/Immunofluorescence: IRF3 Antibody [NBP1-92028] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.")
![Immunohistochemistry-Paraffin: IRF3 Antibody [NBP1-92028] -Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-92028_rabbit-polyclonal-irf3-antibody-immunohistochemistry-paraffin-2410202312550..jpg)
![Western Blot: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ESR1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-CL1196-Western-Blot-NBP2-34478-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ESR1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Staining of MCF7 cells using the Anti-ESR1 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-CL1196-Immunocytochemistry-Immunofluorescence-NBP2-34478-img0009.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Staining of human testis shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-CL1196-Immunohistochemistry-NBP2-34478-img0005.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Staining of human endometrium shows strong nuclear immunoreactivity in glandular cells.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-CL1196-Immunohistochemistry-NBP2-34478-img0001.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Staining of human cervix shows strong nuclear positivity in epithelial cells.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-CL1196-Immunohistochemistry-NBP2-34478-img0002.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Staining of human fallopian tube shows strong immunoreactivity in epithelial cells.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-CL1196-Immunohistochemistry-NBP2-34478-img0003.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Staining of human breast cancer shows moderate to strong nuclear positivity in a subset of tumor cells.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-CL1196-Immunohistochemistry-NBP2-34478-img0004.jpg)
![Immunohistochemistry-Paraffin: YAP1 Antibody (SU33-06) [NBP2-67467] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-YAP1 antibody. Counter stained with hematoxylin. Dilution 1:50-1:200.](http://images.novusbio.com/fullsize/YAP1-Antibody-SU33-06-Immunohistochemistry-Paraffin-NBP2-67467-img0004.jpg "Immunohistochemistry-Paraffin: YAP1 Antibody (SU33-06) [NBP2-67467] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-YAP1 antibody. Counter stained with hematoxylin. Dilution 1:50-1:200.")
![Immunocytochemistry/Immunofluorescence: YAP1 Antibody (SU33-06) [NBP2-67467] - Staining YAP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. Dilution 1:50-1:200.](http://images.novusbio.com/fullsize/YAP1-Antibody-SU33-06-Immunocytochemistry-Immunofluorescence-NBP2-67467-img0002.jpg)
![Western Blot: YAP1 Antibody (SU33-06) [NBP2-67467] - Analysis of YAP1 on HepG2 cell lysates using anti-YAP1 antibody at 1/1,000 dilution. Observed molecular weight 65 kDa.](http://images.novusbio.com/fullsize/YAP1-Antibody-SU33-06-Western-Blot-NBP2-67467-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: YAP1 Antibody (SU33-06) [NBP2-67467] - ICC staining YAP1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. Dilution 1:50-1:200.](http://images.novusbio.com/fullsize/YAP1-Antibody-SU33-06-Immunocytochemistry-Immunofluorescence-NBP2-67467-img0008.jpg)
![Immunohistochemistry-Paraffin: YAP1 Antibody (SU33-06) [NBP2-67467] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-YAP1 antibody. Counter stained with hematoxylin. Dilution 1:50-1:200.](http://images.novusbio.com/fullsize/YAP1-Antibody-SU33-06-Immunohistochemistry-Paraffin-NBP2-67467-img0010.jpg)
![Flow Cytometry: YAP1 Antibody (SU33-06) [NBP2-67467] - Analysis of NIH/3T3 cells with YAP1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/YAP1-Antibody-SU33-06-Flow-Cytometry-NBP2-67467-img0001.jpg)
![Immunohistochemistry-Paraffin: YAP1 Antibody (SU33-06) [NBP2-67467] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-YAP1 antibody. Counter stained with hematoxylin. Dilution1:50-1:200.](http://images.novusbio.com/fullsize/YAP1-Antibody-SU33-06-Immunohistochemistry-Paraffin-NBP2-67467-img0009.jpg)
![Western Blot: RNA Polymerase II/POLR2A [p Ser5] Antibody (C.15200007) [NBP2-59219] - Whole cell extracts (40 ug) from HeLa cells transfected with Pol II siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against Pol II S5p diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser5-Antibody-C-15200007-Western-Blot-NBP2-59219-img0002.jpg "Western Blot: RNA Polymerase II/POLR2A [p Ser5] Antibody (C.15200007) [NBP2-59219] - Whole cell extracts (40 ug) from HeLa cells transfected with Pol II siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against Pol II S5p diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.")
![Chromatin Immunoprecipitation: RNA Polymerase II/POLR2A [p Ser5] Antibody (C.15200007) [NBP2-59219] - ChIP assays were performed using human HeLa cells, the antibody against Pol II S5p and optimized PCR primer pairs for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 1 million cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoter and the coding region of the constitutively expressed GAPDH and ACTB genes, used as positive controls, and for exon 2 of the inactive myoglobin (MB) gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser5-Antibody-C-15200007-Chromatin-Immunoprecipitation-NBP2-59219-img0004.jpg)
![Western Blot: RNA Polymerase II/POLR2A [p Ser5] Antibody (C.15200007) [NBP2-59219] - Nuclear extracts (25 ug) from HeLa cells were analysed by Western blot using the antibody against Pol II S5p diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser5-Antibody-C-15200007-Western-Blot-NBP2-59219-img0001.jpg)
![ELISA: RNA Polymerase II/POLR2A [p Ser5] Antibody (C.15200007) [NBP2-59219] - Cross reactivity of the antibody directed against Pol IIS5p. To test the specificity an ELISA was performed using a serial dilution of the antibody against Pol IIS5p. The wells were coated with peptides containing the unmodified C-terminal repeat sequence as well as different phosphorylated peptides. Figure shows the specificity of the antibody for the S5 phosphorylation.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser5-Antibody-C-15200007-ELISA-NBP2-59219-img0003.jpg)
![Immunofluorescence: RNA Polymerase II/POLR2A [p Ser5] Antibody (C.15200007) [NBP2-59219] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-p-Ser5-Antibody-C-15200007-Immunofluorescence-NBP2-59219-img0005.jpg)
![Western Blot: LEF1 Antibody (3H5) [H00051176-M01] - LEF1 monoclonal antibody (M01), clone 3H5 Analysis of LEF1 expression in MES-SA/Dx5.](http://images.novusbio.com/fullsize/LEF1-Antibody-3H5-Western-Blot-H00051176-M01-img0009.jpg "Western Blot: LEF1 Antibody (3H5) [H00051176-M01] - LEF1 monoclonal antibody (M01), clone 3H5 Analysis of LEF1 expression in MES-SA/Dx5.")
![Western Blot: LEF1 Antibody (3H5) [H00051176-M01] - Analysis of LEF1 over-expressed 293 cell line, cotransfected with LEF1 Validated Chimera RNAi ( Cat # H00051176-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with LEF1 monoclonal antibody (M01), clone 3H5 (Cat # H00051176-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/LEF1-Antibody-3H5-Western-Blot-H00051176-M01-img0006.jpg)
![Western Blot: LEF1 Antibody (3H5) [H00051176-M01] - LEF1 monoclonal antibody (M01), clone 3H5. Analysis of LEF1 expression in HL-60.](http://images.novusbio.com/fullsize/LEF1-Antibody-3H5-Western-Blot-H00051176-M01-img0008.jpg)
![Western Blot: LEF1 Antibody (3H5) [H00051176-M01] - Analysis of LEF1 expression in transfected 293T cell line by LEF1 monoclonal antibody (M01), clone 3H5.Lane 1: LEF1 transfected lysate(44.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/LEF1-Antibody-3H5-Western-Blot-H00051176-M01-img0010.jpg)
![Sandwich ELISA: LEF1 Antibody (3H5) [H00051176-M01] - Detection limit for recombinant GST tagged LEF1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/LEF1-Antibody-3H5-Sandwich-ELISA-H00051176-M01-img0007.jpg)