| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43883 | |||
| BSA Free | NBP2-61436-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72569 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89201-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52636-0.025mg | |||
![Simple Western: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phospho KAP-1 (S824) by Simple Western™. Samples: Whole cell lysate (0.4 mg/mL) from HEK293T cells treated with 100 µM etoposide prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal antibody [BL-246-7B5] (NBP2-76398) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.](http://images.novusbio.com/fullsize/nbp2-76398_rabbit-monoclonal-kap1-p-ser824-antibody-bl-246-7b5-30320239244.jpg "<b>Biological Strategies Validation. </b>Simple Western: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phospho KAP-1 (S824) by Simple Western™. Samples: Whole cell lysate (0.4 mg/mL) from HEK293T cells treated with 100 µM etoposide prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal antibody [BL-246-7B5] (NBP2-76398) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.")
![<b>Biological Strategies Validation. </b>Western Blot: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phospho KAP-1 (S824) by WB of HEK293T lysate treated with 100 uM EPE (+) or mock treated (-). Antibodies: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal [BL-246-7B5] (NBP2-76398). Lower panel shows WB for total KAP-1 using rabbit anti-KAP-1 recombinant monoclonal [BL-248-2G6] (NBP2-76398). Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/KAP1-p-Ser284-Antibody-BL-246-7B5-NBP2-76398-img0001.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phospho KAP1 (S824) in FFPE breast carcinoma by IHC and in FFPE EPE treated HeLa cells by ICC. Mock phosphatase treated sections (left) and calf intestinal phosphatase-treated sections (right). Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal [BL-246-7B5] (NBP2-76398). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/KAP1-p-Ser284-Antibody-BL-246-7B5-Immunocytochemistry-NBP2-76398-img0004.jpg)
![Immunohistochemistry: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phospho KAP-1 (S824) in FFPE lung carcinoma by IHC. Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal [BL-246-7B5] (NBP2-76398). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/KAP1-p-Ser284-Antibody-BL-246-7B5-Immunohistochemistry-NBP2-76398-img0002.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phospho KAP-1 (S824) (red) in formaldehyde-fixed asynchronous HeLa cells treated with EPE (right) or untreated (left) by ICC-IF. Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal [BL-246-7B5] (NBP2-76398). Secondary: DyLight 594-conjugated goat anti-rabbit IgG. Counterstain: Phalloidin conjugated Alexafluor 488 (green).](http://images.novusbio.com/fullsize/KAP1-p-Ser284-Antibody-BL-246-7B5-Immunocytochemistry-Immunofluorescence-NBP2-76398-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phopsho KAP-1 (S824) by WB of immunoprecipitates from HEK293T lysate treated with 100 uM EPE (+) or mock treated (-). Antibodies: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal [BL-246-7B5] (NBP2-76398) and rabbit anti-KAP-1 recombinant monoclonal [BL-248-2G6] (NBP2-76411).](http://images.novusbio.com/fullsize/KAP1-p-Ser284-Antibody-BL-246-7B5-Immunoprecipitation-NBP2-76398-img0005.jpg)
![Flow Cytometry- KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Antibody: Rabbitanti-phospho KAP-1 recombinant monoclonal[BL-246-7B5] or isotype control (unshaded).Secondary: DyLight® 488-conjugated goat anti-rabbit IgG](http://images.novusbio.com/fullsize/nbp2-76398_rabbit-monoclonal-kap1-p-ser824-antibody-bl-246-7b5-flow-cytometry-30112023101053..jpg)
![Western Blot: Nrf2 Antibody (HL1021) [NBP3-13682] - Untreated (-) and treated (+) Neuro2A whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membranes were blotted with Nrf2 antibody [HL1021] (NBP3-13682) diluted at 1:1000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Nrf2-Antibody-HL1021-Western-Blot-NBP3-13682-img0005.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Nrf2 Antibody (HL1021) [NBP3-13682] - Untreated (-) and treated (+) Neuro2A whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membranes were blotted with Nrf2 antibody [HL1021] (NBP3-13682) diluted at 1:1000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunocytochemistry/Immunofluorescence: Nrf2 Antibody (HL1021) [NBP3-13682] - Nrf2 antibody [HL1021] detects Nrf2 protein at nucleus by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nrf2 stained by Nrf2 antibody [HL1021] (NBP3-13682) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Nrf2-Antibody-HL1021-Immunocytochemistry-Immunofluorescence-NBP3-13682-img0004.jpg)
![Immunohistochemistry-Paraffin: Nrf2 Antibody (HL1021) [NBP3-13682] - Human breast carcinoma. Nrf2 stained by Nrf2 antibody [HL1021] (NBP3-13682) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Nrf2-Antibody-HL1021-Immunohistochemistry-Paraffin-NBP3-13682-img0003.jpg)
![Western Blot: Nrf2 Antibody (HL1021) [NBP3-13682] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Nrf2 antibody [HL1021] (NBP3-13682) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13682_rabbit-nrf2-mab-hl1021-910202420122022.jpg)
![Immunohistochemistry-Paraffin: Nrf2 Antibody (HL1021) [NBP3-13682] - Nrf2 antibody [HL1021] detects Nrf2 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissue.Nrf2 stained by Nrf2 antibody [HL1021] (NBP3-13682) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13682_rabbit-nrf2-mab-hl1021-91020242011413.jpg)
![Untreated (-) and treated (+) Rat2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [HL1021] (NBP3-13682) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-13682_rabbit-nrf2-mab-hl1021-western-blot-261120251850308.jpg)
![NRF2 antibody [HL1021] detects NRF2 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse salivary gland.NRF2 stained by NRF2 antibody [HL1021] (NBP3-13682) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13682_rabbit-nrf2-mab-hl1021-immunohistochemistry-paraffin-2611202518484812.jpg)
![Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [HL1020] (NBP3-13682) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13682_rabbit-nrf2-mab-hl1021-western-blot-2611202518484817.jpg)
![Untreated (-) and treated (+) Neuro2A whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [HL1021] (NBP3-13682) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13682_rabbit-nrf2-mab-hl1021-western-blot-261120251839624.jpg)
![Western Blot: POU3F2/OCT7 Antibody (CL6228) [NBP2-61436] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-POU3F2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/POU3F2-OCT7-Antibody-CL6228-Western-Blot-NBP2-61436-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: POU3F2/OCT7 Antibody (CL6228) [NBP2-61436] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-POU3F2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody (CL6228) [NBP2-61436] - Staining of mouse forebrain shows nuclear immunoreactivity in a subset of cells in the lateral ventricle wall.](http://images.novusbio.com/fullsize/POU3F2-OCT7-Antibody-CL6228-Immunohistochemistry-Paraffin-NBP2-61436-img0004.jpg)
![Western Blot: POU3F2/OCT7 Antibody (CL6228) [NBP2-61436] - Analysis in human cerebral cortex tissue.](http://images.novusbio.com/fullsize/POU3F2-OCT7-Antibody-CL6228-Western-Blot-NBP2-61436-img0010.jpg)
![Western Blot: POU3F2/OCT7 Antibody (CL6228) [NBP2-61436] - Analysis in mouse cerebral cortex tissue.](http://images.novusbio.com/fullsize/POU3F2-OCT7-Antibody-CL6228-Western-Blot-NBP2-61436-img0009.jpg)
![Western Blot: POU3F2/OCT7 Antibody (CL6228) [NBP2-61436] - Analysis in human cell line U-251 MG.](http://images.novusbio.com/fullsize/POU3F2-OCT7-Antibody-CL6228-Western-Blot-NBP2-61436-img0008.jpg)
![Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody (CL6228) [NBP2-61436] - Staining of mouse cerebral cortex shows moderate nuclear positivity in neurons in layers 2-4.](http://images.novusbio.com/fullsize/POU3F2-OCT7-Antibody-CL6228-Immunohistochemistry-Paraffin-NBP2-61436-img0002.jpg)
![Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody (CL6228) [NBP2-61436] - Staining of mouse cerebral cortex shows absence of positivity in layer 6 neurons as expected.](http://images.novusbio.com/fullsize/POU3F2-OCT7-Antibody-CL6228-Immunohistochemistry-Paraffin-NBP2-61436-img0003.jpg)
![Western Blot: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAFB.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Western-Blot-NBP2-45718-img0017.jpg "Western Blot: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAFB.")
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Carcinoma of Human lung tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0016.jpg)
![Flow Cytometry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by MAFB antibody.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Flow-Cytometry-NBP2-45718-img0001.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Human breast tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0002.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Carcinoma of Human pancreas tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0003.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Adenocarcinoma of Human ovary tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0004.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Human Ovary tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0005.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0006.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Human pancreas tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0007.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Carcinoma of Human bladder tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0008.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Human lymphoma tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0009.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Human liver tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0010.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Adenocarcinoma of Human breast tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0011.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Human tonsil tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0012.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Human Kidney tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0013.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Carcinoma of Human thyroid tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0014.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Adenocarcinoma of Human colon tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0015.jpg)
![Western Blot: SP1 Antibody (5I3A8) [NBP3-15837] - Western blot analysis of extracts of various cell lines, using SP1 antibody (NBP3-15837) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/SP1-Antibody-5I3A8-Western-Blot-NBP3-15837-img0005.jpg "Western Blot: SP1 Antibody (5I3A8) [NBP3-15837] - Western blot analysis of extracts of various cell lines, using SP1 antibody (NBP3-15837) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: SP1 Antibody (5I3A8) [NBP3-15837] - Immunofluorescence analysis of NIH-3T3 cells using SP1 antibody (NBP3-15837). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SP1-Antibody-5I3A8-Immunocytochemistry-Immunofluorescence-NBP3-15837-img0004.jpg)
![Chromatin Immunoprecipitation: SP1 Antibody (5I3A8) [SP1] - Chromatin immunoprecipitation analysis of extracts of 293T cells, using SP1 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-15837_rabbit-sp1-mab-5i3a8-52202517184575.jpg)
![Chromatin Immunoprecipitation: SP1 Antibody (5I3A8) [SP1] - Chromatin immunoprecipitation was performed with 25 ug of cross-linked chromatin from 293T cells using 5 ug of SP1 Rabbit mAb . DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 . The ChIP sequencing results indicate the enrichment pattern of SP1 across chromosome 14 (upper panel) and the genomic region encompassing TINF2, a representative gene enriched in SP1 (lower panel).](http://images.novusbio.com/fullsize/nbp3-15837_rabbit-sp1-mab-5i3a8-5220251726072.jpg)
![Immunohistochemistry: SP1 Antibody (5I3A8) [SP1] - Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using SP1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15837_rabbit-sp1-mab-5i3a8-52202517162322.jpg)
![Immunohistochemistry: SP1 Antibody (5I3A8) [SP1] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using SP1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15837_rabbit-sp1-mab-5i3a8-5220251718937.jpg)
![Immunohistochemistry: SP1 Antibody (5I3A8) [SP1] - Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using SP1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15837_rabbit-sp1-mab-5i3a8-5220251726088.jpg)
![Chromatin Immunoprecipitation: SP1 Antibody (5I3A8) [SP1] - Chromatin immunoprecipitation was performed with 25 ug of cross-linked chromatin from 293T cells using 5 ug of SP1 Rabbit mAb . DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 . The ChIP sequencing results indicate the enrichment pattern of SP1 in the representative genomic region surrounding TINF2 gene.](http://images.novusbio.com/fullsize/nbp3-15837_rabbit-sp1-mab-5i3a8-52202517202936.jpg)
![Immunohistochemistry: SP1 Antibody (5I3A8) [SP1] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using SP1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15837_rabbit-sp1-mab-5i3a8-52202517273517.jpg)
![Immunohistochemistry: SP1 Antibody (5I3A8) [SP1] - Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using SP1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15837_rabbit-sp1-mab-5i3a8-52202517192996.jpg)
![Immunohistochemistry: SP1 Antibody (5I3A8) [SP1] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using SP1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15837_rabbit-sp1-mab-5i3a8-52202517251978.jpg)
![Immunocytochemistry/ Immunofluorescence: SP1 Antibody (5I3A8) [SP1] - Confocal imaging of C6 cells using SP1 Rabbit mAb . The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15837_rabbit-sp1-mab-5i3a8-52202517213553.jpg)
![Western Blot: YB1 Antibody (9J7M4) [NBP3-16213] - Analysis of extracts of various cell lines, using YB1 Rabbit mAb (NBP3-16213) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/YB1-Antibody-9J7M4-Western-Blot-NBP3-16213-img0008.jpg "Western Blot: YB1 Antibody (9J7M4) [NBP3-16213] - Analysis of extracts of various cell lines, using YB1 Rabbit mAb (NBP3-16213) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: YB1 Antibody (9J7M4) [NBP3-16213] - Analysis of extracts of HeLa cells, using YB1 Rabbit mAb (NBP3-16213) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/YB1-Antibody-9J7M4-Western-Blot-NBP3-16213-img0007.jpg)
![Immunoprecipitation: YB1 Antibody (9J7M4) [NBP3-16213] - Immunoprecipitation of YB1 from 200 ug extracts of HeLa cells was performed using 0.5 ug of YB1 Rabbit mAb . Rabbit IgG isotype control was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using YB1 Rabbit mAb at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-16213_rabbit-yb1-mab-9j7m4-5220251255573.jpg)
![Immunocytochemistry/ Immunofluorescence: YB1 Antibody (9J7M4) [NBP3-16213] - Confocal imaging of NIH/3T3 cells using YB1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16213_rabbit-yb1-mab-9j7m4-52202511361248.jpg)
![Immunohistochemistry: YB1 Antibody (9J7M4) [NBP3-16213] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using YB1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16213_rabbit-yb1-mab-9j7m4-5220251215454.jpg)
![Immunohistochemistry: YB1 Antibody (9J7M4) [NBP3-16213] - Immunohistochemistry analysis of paraffin-embedded Human colon using YB1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16213_rabbit-yb1-mab-9j7m4-52202511374130.jpg)
![Immunohistochemistry: YB1 Antibody (9J7M4) [NBP3-16213] - Immunohistochemistry analysis of paraffin-embedded Rat kidney using YB1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16213_rabbit-yb1-mab-9j7m4-52202511411489.jpg)
![Immunohistochemistry: YB1 Antibody (9J7M4) [NBP3-16213] - Immunohistochemistry analysis of paraffin-embedded Rat colon using YB1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16213_rabbit-yb1-mab-9j7m4-5220251140277.jpg)
![Immunohistochemistry: YB1 Antibody (9J7M4) [NBP3-16213] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using YB1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16213_rabbit-yb1-mab-9j7m4-52202511392221.jpg)
![Immunocytochemistry/ Immunofluorescence: YB1 Antibody (9J7M4) [NBP3-16213] - Confocal imaging of U-2 OS cells using YB1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16213_rabbit-yb1-mab-9j7m4-52202511374198.jpg)
![Western Blot: TDP-43/TARDBP Antibody (4R5L7) [NBP3-15677] - Western blot analysis of extracts of various cell lines, using TDP-43/TARDBP antibody (NBP3-15677) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-4R5L7-Western-Blot-NBP3-15677-img0007.jpg "Western Blot: TDP-43/TARDBP Antibody (4R5L7) [NBP3-15677] - Western blot analysis of extracts of various cell lines, using TDP-43/TARDBP antibody (NBP3-15677) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (4R5L7) [NBP3-15677] - Immunohistochemistry of paraffin-embedded mouse brain using TDP-43/TARDBP Rabbit mAb (NBP3-15677) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-4R5L7-Immunohistochemistry-Paraffin-NBP3-15677-img0003.jpg)
![Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (4R5L7) [NBP3-15677] - Immunohistochemistry of paraffin-embedded rat ovary using TDP-43/TARDBP Rabbit mAb (NBP3-15677) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-4R5L7-Immunohistochemistry-Paraffin-NBP3-15677-img0001.jpg)
![Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (4R5L7) [NBP3-15677] - Immunohistochemistry of paraffin-embedded human esophageal using TDP-43/TARDBP Rabbit mAb (NBP3-15677) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-4R5L7-Immunohistochemistry-Paraffin-NBP3-15677-img0002.jpg)
![Immunohistochemistry: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Immunohistochemistry analysis of paraffin-embedded Mouse testis using TDP-43/TARDBP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-52202517315540.jpg)
![Immunocytochemistry/ Immunofluorescence: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Confocal imaging of HeLa cells using TDP-43/TARDBP Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-52202517352713.jpg)
![Immunoprecipitation: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Immunoprecipitation of TDP-43/TARDBP from 500 ug extracts of K-562 cells was performed using 2 ug of TDP-43/TARDBP Rabbit mAb . Rabbit IgG isotype control was used to precipitate the Control IgG sample. IP samples were eluted with 1X non-reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using TDP-43/TARDBP Rabbit mAb at a dilution of 1:500.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-5220251734597.jpg)
![Chromatin Immunoprecipitation: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Chromatin immunoprecipitation analysis of extracts of K562 cells, using TDP-43/TARDBP Rabbit mAb and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-52202517344289.jpg)
![Immunocytochemistry/ Immunofluorescence: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Confocal imaging of paraffin-embedded Mouse brain tissue using TDP-43/TARDBP Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-52202517363742.jpg)
![Immunohistochemistry: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Immunohistochemistry analysis of paraffin-embedded Mouse colon using TDP-43/TARDBP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-52202517361227.jpg)
![Immunohistochemistry: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Immunohistochemistry analysis of paraffin-embedded Human colon using TDP-43/TARDBP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-52202517315511.jpg)
![Immunohistochemistry: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using TDP-43/TARDBP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-52202517311072.jpg)
![Immunohistochemistry: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Immunohistochemistry analysis of paraffin-embedded Rat liver using TDP-43/TARDBP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-52202517304973.jpg)
![Western Blot: YAP1 Antibody (10G4S10) [YAP1] - Western blot analysis of lysates from wild type (WT) and YAP1 knockout (KO) HeLa cells using [KO Validated] YAP1 Rabbit mAb at 1:10000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15685_rabbit-yap1-mab-10g4s10-52202517304998.jpg "<b>Genetic Strategies Validation. </b>Western Blot: YAP1 Antibody (10G4S10) [YAP1] - Western blot analysis of lysates from wild type (WT) and YAP1 knockout (KO) HeLa cells using [KO Validated] YAP1 Rabbit mAb at 1:10000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: YAP1 Antibody (10G4S10) [YAP1] - Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] YAP1 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15685_rabbit-yap1-mab-10g4s10-52202517315554.jpg)
![Immunohistochemistry: YAP1 Antibody (10G4S10) [YAP1] - Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma tissue using [KO Validated] YAP1 Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15685_rabbit-yap1-mab-10g4s10-5220251733585.jpg)
![Immunohistochemistry: YAP1 Antibody (10G4S10) [YAP1] - Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using [KO Validated] YAP1 Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15685_rabbit-yap1-mab-10g4s10-52202517304960.jpg)
![Immunocytochemistry/ Immunofluorescence: YAP1 Antibody (10G4S10) [YAP1] - Immunofluorescence analysis of HeLa cells using [KO Validated] YAP1 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15685_rabbit-yap1-mab-10g4s10-5220251736120.jpg)
![Immunohistochemistry: YAP1 Antibody (10G4S10) [YAP1] - Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using [KO Validated] YAP1 Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15685_rabbit-yap1-mab-10g4s10-5220251733574.jpg)
![Immunohistochemistry: YAP1 Antibody (10G4S10) [YAP1] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using [KO Validated] YAP1 Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15685_rabbit-yap1-mab-10g4s10-52202517352720.jpg)
![Immunohistochemistry: YAP1 Antibody (10G4S10) [YAP1] - Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using [KO Validated] YAP1 Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15685_rabbit-yap1-mab-10g4s10-52202517352748.jpg)
![Immunoprecipitation: YAP1 Antibody (10G4S10) [YAP1] - Immunoprecipitation analysis of 300 ug extracts of A-549 cells using 3 ug YAP1 antibody . Western blot was performed from the immunoprecipitate using YAP1 antibody at a dilution of 1:500.](http://images.novusbio.com/fullsize/nbp3-15685_rabbit-yap1-mab-10g4s10-52202517315557.jpg)
![Immunocytochemistry/ Immunofluorescence: YAP1 Antibody (10G4S10) [YAP1] - Immunofluorescence analysis of U20S cells using [KO Validated] YAP1 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15685_rabbit-yap1-mab-10g4s10-5220251733562.jpg)
![Immunohistochemistry: YAP1 Antibody (10G4S10) [NBP3-15685] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KO Validated] YAP1 Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15685_rabbit-yap1-mab-10g4s10-822025209898.jpg)
![Western Blot: IRF7 Antibody [NBP3-06987] - Loading: 15 ug of lysates per lane. Antibodies: NBP3-06987(1 ug/mL), 1 hr incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution](http://images.novusbio.com/fullsize/IRF7-Antibody-Western-Blot-NBP3-06987-img0005.jpg "Western Blot: IRF7 Antibody [NBP3-06987] - Loading: 15 ug of lysates per lane. Antibodies: NBP3-06987(1 ug/mL), 1 hr incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution")
![Western Blot: IRF7 Antibody [NBP3-06987] - Loading: 10ug of A549 WT cell lysates or IRF7 KO cell lysates. Antibodies: NBP3-06987 (0.5 ug/mL) and beta-actin, 1 hr incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/IRF7-Antibody-Western-Blot-NBP3-06987-img0001.jpg)
![Western Blot: IRF7 Antibody [NBP3-06987] - Loading: 15 ug of lysates per lane. Antibodies: NBP3-06987(1 ug/mL), 1 hr incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution](http://images.novusbio.com/fullsize/IRF7-Antibody-Western-Blot-NBP3-06987-img0002.jpg)
![Western Blot: IRF7 Antibody [NBP3-06987] - Loading: 15 ug of lysates per lane. Antibodies: NBP3-06987(1 ug/mL), 1 hr incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution](http://images.novusbio.com/fullsize/IRF7-Antibody-Western-Blot-NBP3-06987-img0003.jpg)
![Western Blot: IRF7 Antibody [NBP3-06987] - Loading: 15 ug of lysates per lane. Antibodies: NBP3-06987(1 ug/mL), 1 hr incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution](http://images.novusbio.com/fullsize/IRF7-Antibody-Western-Blot-NBP3-06987-img0004.jpg)
![Immunohistochemistry: IRF7 Antibody - BSA Free [NBP3-06987] - Validation of IRF7 in Mouse Pancreas Tissue. Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-IRF7 antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp3-06987_rabbit-polyclonal-irf7-antibody-271120241937745.gif)
![Immunohistochemistry: IRF7 Antibody - BSA Free [NBP3-06987] - Validation of IRF7 in Rat Brain Tissue.Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-IRF7 antibody at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp3-06987_rabbit-polyclonal-irf7-antibody-271120242032617.gif)
![Immunohistochemistry: IRF7 Antibody - BSA Free [NBP3-06987] - Validation of IRF7 in Human Liver Tissue.Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-IRF7 antibody at 2 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp3-06987_rabbit-polyclonal-irf7-antibody-2711202420285873.gif)
![Immunohistochemistry: IRF7 Antibody - BSA Free [NBP3-06987] - Validation of IRF7 in Human Colon Tissue.Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-IRF7 antibody at 2 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp3-06987_rabbit-polyclonal-irf7-antibody-271120241955301.gif)
![Immunohistochemistry: IRF7 Antibody [NBP3-06987] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue using NBP3-06987 at 2 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degrees C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/IRF7-Antibody-Immunohistochemistry-NBP3-06987-img0008.jpg)
![Immunohistochemistry: IRF7 Antibody - BSA Free [NBP3-06987] - Validation of IRF7 in Rat Brain Tissue.Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-IRF7 antibody at 5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp3-06987_rabbit-polyclonal-irf7-antibody-2711202419392750.gif)
![Immunocytochemistry/ Immunofluorescence: IRF7 Antibody - BSA Free [NBP3-06987] - Validation of IRF7 in Mouse Raw264.7 Cells.Immunofluorescent analysis of 4% paraformaldehyde-fixed Raw264.7 cells labeling IRF7 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp3-06987_rabbit-polyclonal-irf7-antibody-2711202420272475.gif)
![Western Blot: Olig2 Antibody (064) [NBP2-89201] - A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:32 kDa Observed band size:37 kDa](http://images.novusbio.com/fullsize/Olig2-Antibody-064-Western-Blot-NBP2-89201-img0003.jpg "Western Blot: Olig2 Antibody (064) [NBP2-89201] - A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:32 kDa Observed band size:37 kDa")
![Immunohistochemistry-Paraffin: Olig2 Antibody (064) [NBP2-89201] - Staining of human olig-2 in human brain with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/Olig2-Antibody-064-Immunohistochemistry-Paraffin-NBP2-89201-img0002.jpg)
![Immunohistochemistry-Paraffin: Olig2 Antibody (064) [NBP2-89201] - Staining of human olig-2 in human glioma with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/Olig2-Antibody-064-Immunohistochemistry-Paraffin-NBP2-89201-img0001.jpg)
![Flow Cytometry: Rabbit Monoclonal Olig2 Antibody (064) [NBP2-89201] - Mouse brain cells fixed and stained for Olig2 protein: 1:100. AF405 conjugated version of antibody used (Catalog # NBP2-89201AF405). Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp2-89201_rabbit-monoclonal-olig2-antibody-064-flow-cytometry-1052024144135..png)
![Flow Cytometry: Rabbit Monoclonal Olig2 Antibody (064) [NBP2-89201] - Rat brain cells fixed and stained with Olig2. AF405 conjugated version of antibody used (Catalog # NBP2-89201AF405). Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp2-89201_rabbit-monoclonal-olig2-antibody-064-flow-cytometry-1052024144528..png)
![Western Blot: IRF3 Antibody [NBP1-78769] - Whole cell lysate from mouse bone marrow dendritic (D; 50 and 15 mcg for WB; 1 mg for IP, 20% of IP loaded), and NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-IRF3 antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. IRF3 was also immunoprecipitated by rabbit anti-IRF3 antibody NBP1-78768 which recognizes a disparate epitope.](http://images.novusbio.com/fullsize/IRF3-Antibody-Western-Blot-NBP1-78769-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: IRF3 Antibody [NBP1-78769] - Whole cell lysate from mouse bone marrow dendritic (D; 50 and 15 mcg for WB; 1 mg for IP, 20% of IP loaded), and NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-IRF3 antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. IRF3 was also immunoprecipitated by rabbit anti-IRF3 antibody NBP1-78768 which recognizes a disparate epitope.")
