| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-11304-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24941SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25147SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1598SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33060 | |||
| IHC-Prediluted | NBP2-44830 | |||
![Western Blot: alpha Tubulin 4a Antibody (B-5-1-2) - BSA Free [NB120-11304] - Cell line lysates were separated on SDS-PAGE and probed with Monoclonal Anti-alpha-Tubulin Clone: B-5-1-2. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes: 1.) HeLa, 2.) JURKAT, 3.) COS7, 4.) NIH-3T3, 5.) PC-12, 6.) RAT2, 7.) CHO, 8.) MDBK, 9.) MDCK](http://images.novusbio.com/fullsize/alpha-Tubulin-4a-Antibody-B-5-1-2-BSA-Free-Western-Blot-NB120-11304-img0004.jpg "Western Blot: alpha Tubulin 4a Antibody (B-5-1-2) - BSA Free [NB120-11304] - Cell line lysates were separated on SDS-PAGE and probed with Monoclonal Anti-alpha-Tubulin Clone: B-5-1-2. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes: 1.) HeLa, 2.) JURKAT, 3.) COS7, 4.) NIH-3T3, 5.) PC-12, 6.) RAT2, 7.) CHO, 8.) MDBK, 9.) MDCK")
![Immunocytochemistry/Immunofluorescence: alpha Tubulin 4a Antibody (B-5-1-2) [NB120-11304] - Staining of HEK293T cells transfected with a recombinant protein HA tagged, detected with Anti-HA Mouse primary antibody and Anti-Mouse Alexa543 secondary antibody. Microtubules detected with Anti-alpha-tubulin Mouse primary antibody and Anti-Mouse Alexa488 secondary antibody.](http://images.novusbio.com/fullsize/alpha-Tubulin-4a-Antibody-B-5-1-2-Immunofluorescence-NB120-11304-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: alpha Tubulin 4a Antibody (B-5-1-2) [NB120-11304] - CFB cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with Monoclonal Anti-alpha-Tubulin antibody produced in mouse clone No. B-5-1-2. The antibody was developed using Anti-Mouse IgG (Fab specific)-FITC antibody produced in goat. Cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/alpha-Tubulin-4a-Antibody-B-5-1-2-Immunofluorescence-NB120-11304-img0001.jpg)
![alpha Tubulin 4a Antibody (B-5-1-2) - BSA Free [NB120-11304] - Chicken fibroblasts cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1 ug/mL Anti-alpha-Tubulin antibody, Mouse monoclonal, Clone: DM1A (A). The antibody was developed using 1:40 Goat Anti-Mouse IgG (Fab specific)-FITC antibody and 1 ug/mL Monoclonal Anti-alpha-Tubulin antibody produced in Mouse, Clone: B-5-1-2 (B). The antibody was developed using 1:40 Goat Anti-Mouse IgG (Fab specific)-FITC antibodyResults:Two Anti-alpha-Tubulin antibodies, T6199 (A) and T6074 (B) target different regions of alpha-Tubulin show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.](http://images.novusbio.com/fullsize/alpha-Tubulin-4a-Antibody-B-5-1-2-BSA-Free-NB120-11304-img0005.jpg)
![Western Blot: Actin Antibody (JJ09-29) [NBP2-67907] - Analysis of Actin on different lysates with Rabbit anti-Actin antibody at 1/500 dilution.Lane 1: Hela cell lysate (10 ug/Lane)Lane 2: A431 cell lysate (10 ug/Lane)Lane 3: PC-12 cell lysate (10 ug/Lane)Lane 4: Mouse brain tissue lysate (20 ug/Lane)Lane 5: Rat kidney tissue lysate (20 ug/Lane)Predicted band size: 42 kDaObserved band size: 42 kDaExposure time: 30 seconds;10% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-80) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Western-Blot-NBP2-67907-img0008.jpg "Western Blot: Actin Antibody (JJ09-29) [NBP2-67907] - Analysis of Actin on different lysates with Rabbit anti-Actin antibody at 1/500 dilution.Lane 1: Hela cell lysate (10 ug/Lane)Lane 2: A431 cell lysate (10 ug/Lane)Lane 3: PC-12 cell lysate (10 ug/Lane)Lane 4: Mouse brain tissue lysate (20 ug/Lane)Lane 5: Rat kidney tissue lysate (20 ug/Lane)Predicted band size: 42 kDaObserved band size: 42 kDaExposure time: 30 seconds;10% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-80) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Actin Antibody (JJ09-29) [NBP2-67907] - Staining Actin in AGS cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Immunocytochemistry-Immunofluorescence-NBP2-67907-img0006.jpg)
![Immunohistochemistry-Paraffin: Actin Antibody (JJ09-29) [NBP2-67907] - Immunofluorescence analysis of paraffin-embedded human colon carcinoma tissue labeling Actin with Rabbit anti-Actin antibody washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Immunohistochemistry-Paraffin-NBP2-67907-img0009.jpg)
![Western Blot: Actin Antibody (JJ09-29) [NBP2-67907] - Analysis of Actin on different lysates using anti-Actin antibody at 1/1,000 dilution. Positive control: Lane 1: zebrafish Lane 2: Hela Lane 3: A431 Lane 4: Jurkat](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Western-Blot-NBP2-67907-img0005.jpg)
![Western Blot: Actin Antibody (JJ09-29) [NBP2-67907] - Analysis of Actin on different lysates using anti-Actin antibody at 1/1,000 dilution. Positive control: Lane 1:Hybrid fish (crucian-carp) brain tissue Lane 2:Hybrid fish (crucian-carp) kidney tissue](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Western-Blot-NBP2-67907-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Actin Antibody (JJ09-29) [NBP2-67907] - Staining Actin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Immunocytochemistry-Immunofluorescence-NBP2-67907-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Actin Antibody (JJ09-29) [NBP2-67907] - Staining Actin in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Immunocytochemistry-Immunofluorescence-NBP2-67907-img0002.jpg)
![Immunohistochemistry-Paraffin: Actin Antibody (JJ09-29) [NBP2-67907] - Analysis of paraffin-embedded mouse cardiac muscle tissue using anti-Actin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Immunohistochemistry-Paraffin-NBP2-67907-img0003.jpg)
![Immunohistochemistry-Paraffin: Actin Antibody (JJ09-29) [NBP2-67907] - Analysis of paraffin-embedded mouse smooth muscle tissue using anti-Actin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Immunohistochemistry-Paraffin-NBP2-67907-img0004.jpg)
![Western Blot: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of CD34 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: TF-1 cell lysate Lane 2: Mouse lung tissue lysate](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Western-Blot-NBP2-67399-img0007.jpg "Western Blot: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of CD34 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: TF-1 cell lysate Lane 2: Mouse lung tissue lysate")
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - (G) CD34 + microvascular staining in EOC tissue. Correlation between HE4 serum levels and CD34 + area in patient EOC tissue (n = 14). **p < 0.005, ****p < 0.0005. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-020-65353-x), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0008.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded rat kidney tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0003.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human kidney tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0004.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded mouse kidney tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0006.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human tonsil tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0005.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human tonsil tissue labeling CD34 with Rabbit anti-CD34 antibody washed with PBS, and then probed with the primary antibody green) at 1/50 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0009.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0010.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human placenta tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0011.jpg)
![Flow Cytometry: CD20 Antibody (MEM-97) - Azide Free [NBP1-44634] - Double staining of human, canine and porcine B lymphocytes with anti-CD79a (HM57) and anti-CD20 (MEM-97) antibody.](http://images.novusbio.com/fullsize/CD20-Antibody-MEM-97-Azide-Free-Flow-Cytometry-NBP1-44634-img0009.jpg "Flow Cytometry: CD20 Antibody (MEM-97) - Azide Free [NBP1-44634] - Double staining of human, canine and porcine B lymphocytes with anti-CD79a (HM57) and anti-CD20 (MEM-97) antibody.")
![Flow Cytometry: CD20 Antibody (MEM-97) - Azide Free [NBP1-44634] - A cell surface stain was performed on Raji cells with MS4A1/CD20 antibody (MEM-97) NBP1-44634AF488 (blue) along with a matched isotype control NBP2-27287AF488 (orange). Cells were incubated in an antibody dilution of 10 ug/mL for 20 minutes at room temperature.](http://images.novusbio.com/fullsize/CD20-Antibody-MEM-97-Azide-Free-Flow-Cytometry-NBP1-44634-img0001.jpg)
![Flow Cytometry: CD20 Antibody (MEM-97) - Azide Free [NBP1-44634] - A cell surface stain was performed on Daudi cells with MS4A1/CD20 (MEM-97) antibody NBP1-44634PE (blue) along with a matched isotype control NBP2-27287PE (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/CD20-Antibody-MEM-97-Azide-Free-Flow-Cytometry-NBP1-44634-img0002.jpg)
![Flow Cytometry: CD20 Antibody (MEM-97) - Azide Free [NBP1-44634] - Double staining of human, canine and porcine B lymphocytes with anti-CD79a (HM57) and anti-CD20 (MEM-97) antibod](http://images.novusbio.com/fullsize/CD20-Antibody-MEM-97-Azide-Free-Flow-Cytometry-NBP1-44634-img0006.jpg)
![Flow Cytometry: CD20 Antibody (MEM-97) - Azide Free [NBP1-44634] - Flow Cytometry: MS4A1/CD20 Antibody (MEM-97) [Alexa Fluor 647] [NBP1-44634AF647] - A surface stain was performed on human peripheral blood lymphocytes with MS4A1/CD20 (MEM-97) antibody NBP1-44634AF647 and a matched isotype control NBP2-27287AF647. Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-26646. Image using the Alexa Fluor 647 form of this antibody.](http://images.novusbio.com/fullsize/CD20-Antibody-MEM-97-Azide-Free-Flow-Cytometry-NBP1-44634-img0007.jpg)
![Flow Cytometry: CD20 Antibody (MEM-97) - Azide Free [NBP1-44634] - A cell surface stain was performed on Ramos cells with CD20 antibody (MEM-97) NBP1-44634PE (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythin.](http://images.novusbio.com/fullsize/CD20-Antibody-MEM-97-Azide-Free-Flow-Cytometry-NBP1-44634-img0008.jpg)
![Immunoprecipitation: CD20 Antibody (MEM-97) - Azide Free [NBP1-44634] - Immunoprecipitation of human CD20 from the whole cell lysate of RAJI human Burkitt lymphoma cell line. Western blot was immunostained with anti-CD20 rabbit polyclonal antibody. Lane 1: original lysate of RAJI cells Lane 2: immunoprecipitate by Isotypic mouse IgG1 control Lane 3: original lysate of RAJI cells Lane 4: immunoprecipitate by anti-CD20 (MEM-97)](http://images.novusbio.com/fullsize/CD20-Antibody-MEM-97-Azide-Free-Immunoprecipitation-NBP1-44634-img0005.jpg)
![Western Blot: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - Analysis of Chk2 in A) human HEK293 and B) mouse NIH3T3 lysate using Chk2 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Azide-Free-Western-Blot-NBP2-27403-img0003.jpg "Western Blot: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - Analysis of Chk2 in A) human HEK293 and B) mouse NIH3T3 lysate using Chk2 antibody at 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Chk2 (73C175.1.1) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image using the standard format of this product.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-27403-img0006.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - Staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Azide-Free-Immunohistochemistry-Paraffin-NBP2-27403-img0002.jpg)
![Flow Cytometry: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - An intracellular stain was performed on Hek293 cells with Chk2 [73C175.1.1] Antibody NB100-56546 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550. Image using the standard format of this product.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Azide-Free-Flow-Cytometry-NBP2-27403-img0005.jpg)
![Western Blot: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - analysis of Chk2 in 293 cell lysate. A protein band of approximate molecular weight of 60-62 kDa is detected with Chk2 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Azide-Free-Western-Blot-NBP2-27403-img0001.jpg)
![Western Blot: Nanog Antibody [NBP2-24941] - Analysis of Nanog in (A) HeLa and (B) NIH 3T3 cell lysate using NBP2-24941 at 2 ug/ml.](http://images.novusbio.com/fullsize/Nanog-Antibody-Western-Blot-NBP2-24941-img0002.jpg "Western Blot: Nanog Antibody [NBP2-24941] - Analysis of Nanog in (A) HeLa and (B) NIH 3T3 cell lysate using NBP2-24941 at 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: Nanog Antibody [NBP2-24941] - Nanog antibody was tested in Ntera2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image objective 40x. An antibody dilution of 1:10 was used.](http://images.novusbio.com/fullsize/Nanog-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24941-img0005.jpg)
![Western Blot: Nanog Antibody [NBP2-24941] - Protein expression of pluripotency markers in uterine cells isolated from control cows and from cows with adenomyosis. MM - molecular weight marker, C - cells obtained from control cows, ADENO - cells obtained from cows with adenomyosis. Image collected and cropped by CiteAb from the following publication (//rbej.biomedcentral.com/articles/10.1186/s12958-015-0106-0), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nanog-Antibody-Western-Blot-NBP2-24941-img0006.jpg)
![Immunohistochemistry: Nanog Antibody [NBP2-24941] - Immunodetection of pluripotency markers in uterine tissues from control cows & from cows with adenomyosis. a, b – haematoxylin & eosin stained slides of control & adenomyotic uterus, respectively; c, d – NANOG immunodetection in control & adenomyotic tissue, respectively; e, f – immunolocalisation of OCT4 in normal & adenomyotic tissue, respectively; g, h – SOX2 immunodetection in control & adenomyotic tissue, respectively; i, j, k – no Ab, negative controls for NANOG, OCT4 & SOX2, respectively. Unspecific IgG controls (pictures not shown) served similar pictures as no Ab control. Arrows indicate the most intense histochemical reactions; dotted line indicate endometrial-myometrial border; e – endometrium, m – myometrium, es – endometrial stroma, eg – endometrial gland, a – adenomyotic lesion, v – vessel. Scale bars: 20 μm Image collected & cropped by CiteAb from the following publication (//www.rbej.com/content/13/1/110), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-24941_rabbit-polyclonal-nanog-antibody-310202416121911.jpg)
![Western Blot: MCAM/CD146 Antibody [NBP2-15778] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with CD146 antibody [C1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/MCAM-CD146-Antibody-Western-Blot-NBP2-15778-img0013.jpg "Western Blot: MCAM/CD146 Antibody [NBP2-15778] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with CD146 antibody [C1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: MCAM/CD146 Antibody [NBP2-15778] - A. 50 ug rat liver extract 7.5 % SDS-PAGE CD146 antibody [C1C3] dilution: 1:1000](http://images.novusbio.com/fullsize/MCAM-CD146-Antibody-Western-Blot-NBP2-15778-img0007.jpg)
![Western Blot: MCAM/CD146 Antibody [NBP2-15778] - A. 30 ug HUVEC whole cell lysate/extract 7.5 % SDS-PAGE CD146 antibody [C1C3] dilution: 1:10000](http://images.novusbio.com/fullsize/MCAM-CD146-Antibody-Western-Blot-NBP2-15778-img0010.jpg)
![Immunohistochemistry-Paraffin: MCAM/CD146 Antibody [NBP2-15778] - J5 xenograft, using CD146 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MCAM-CD146-Antibody-Immunohistochemistry-Paraffin-NBP2-15778-img0004.jpg)
![Immunohistochemistry-Paraffin: MCAM/CD146 Antibody [NBP2-15778] - Mouse colon. CD146 antibody [C1C3] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MCAM-CD146-Antibody-Immunohistochemistry-Paraffin-NBP2-15778-img0005.jpg)
![Immunohistochemistry-Paraffin: MCAM/CD146 Antibody [NBP2-15778] - Mouse skin. CD146 antibody [C1C3] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MCAM-CD146-Antibody-Immunohistochemistry-Paraffin-NBP2-15778-img0006.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: MCAM/CD146 Antibody [NBP2-15778] - Wild-type (WT) and CD146 knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with CD146 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/MCAM-CD146-Antibody-Western-Blot-NBP2-15778-img0014.jpg)
![CD146 antibody [C1C3] detects CD146 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded mouse placenta.Green: CD146 stained by CD146 antibody [C1C3] (NBP2-15778) diluted at 1:250.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-15778_rabbit-polyclonal-mcam-cd146-antibody-immunohistochemistry-paraffin-2611202518431916.jpg)
![CD146 antibody [C1C3] detects CD146 protein at cell membrane and cytoplasm by immunofluorescent analysis.Sample: HUVEC cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CD146 protein stained by CD146 antibody [C1C3] (NBP2-15778) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-15778_rabbit-polyclonal-mcam-cd146-antibody-immunocytochemistry-immunofluorescence-261120251840488.jpg)
![Immunohistochemistry-Paraffin: OCT4 Antibody [NBP2-15053] - Oct4 antibody detects Oct4 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma. Oct4 stained by Oct4 antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/OCT4-Antibody-Immunohistochemistry-Paraffin-Negative-NBP2-15053-img0015.jpg "Immunohistochemistry-Paraffin: OCT4 Antibody [NBP2-15053] - Oct4 antibody detects Oct4 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma. Oct4 stained by Oct4 antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Flow Cytometry: OCT4 Antibody [NBP2-15053] - Human embryonic stem cells, staining with OCT3/4 or rabbit IgG (black).](http://images.novusbio.com/fullsize/OCT4-Antibody-Flow-Cytometry-NBP2-15053-img0001.jpg)
![Western Blot: OCT4 Antibody [NBP2-15053] - Sample: cell lysate/extract of OCT4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell, B. Control with 2 ug of preimmune rabbit IgG, C. Immunoprecipitation of OCT4 by 2 ug of OCT4 antibody 12% SDS-PAGE gel.](http://images.novusbio.com/fullsize/OCT4-Antibody-Western-Blot-NBP2-15053-img0007.jpg)
![Western Blot: OCT4 Antibody [NBP2-15053] - Sample (20 ug of whole cell lysate) A: mouse ESC 12% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/OCT4-Antibody-Western-Blot-NBP2-15053-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: OCT4 Antibody [NBP2-15053] - Analysis of hESCs on CF1 feeders, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/OCT4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15053-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: OCT4 Antibody [NBP2-15053] - Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody diluted at 1:200000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15053_rabbit-polyclonal-oct4-antibody-1610202419414555.jpg)
![Western Blot: Enolase 1 Antibody (253) [NBP2-25147] - Analysis of different cell lysates using mouse mAb to alpha-enolase, NBP2-25147, dilution 1:10,000 in green: [1] protein standard (red), [2] NIH-3T3 l, [3] C6, [4] HEK293, [5] HeLa, and [6] SH-SY5Y cells. A strong single band at 47kDa corresponds to the alpha-enolase protein.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-253-Western-Blot-NBP2-25147-img0008.jpg "Western Blot: Enolase 1 Antibody (253) [NBP2-25147] - Analysis of different cell lysates using mouse mAb to alpha-enolase, NBP2-25147, dilution 1:10,000 in green: [1] protein standard (red), [2] NIH-3T3 l, [3] C6, [4] HEK293, [5] HeLa, and [6] SH-SY5Y cells. A strong single band at 47kDa corresponds to the alpha-enolase protein.")
![Immunocytochemistry/Immunofluorescence: Enolase 1 Antibody (253) [NBP2-25147] - Analysis of HeLa cells stained with mouse mAb to alpha-enolase, NBP2-25147, dilution 1:500 in green and costained with chicken pAb to HSP60, dilution 1:5,000, in red. The blue is DAPI staining of nuclear DNA. NBP2-25147 antibody reveals strong cytoplasmic staining.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-253-Immunocytochemistry-Immunofluorescence-NBP2-25147-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Enolase 1 Antibody (253) [NBP2-25147] - Rat 3T3 cells stained with NBP2-25147 (green) and counterstained with chicken polyclonal antibody to Vimentin NB300-223 (red) and DNA (blue). NBP2-25147 reveals strong cytoplasmic staining, while the Vimentin antibody reveals cytoplasmic intermediate filaments.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-253-Immunocytochemistry-Immunofluorescence-NBP2-25147-img0003.jpg)
![Simple Western: Enolase 1 Antibody (253) [NBP2-25147] - Simple Western lane view shows a specific band for Enolase 1 in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-253-Simple-Western-NBP2-25147-img0006.jpg)
![Western Blot: CRIPTO Antibody [NB100-1598] - Total protein from human HeLa, NTERA-2 and spleen and embryonic mouse V6.5 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Cripto in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/CRIPTO-Antibody-Western-Blot-NB100-1598-img0013.jpg "Western Blot: CRIPTO Antibody [NB100-1598] - Total protein from human HeLa, NTERA-2 and spleen and embryonic mouse V6.5 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Cripto in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: CRIPTO Antibody [NB100-1598] - CRIPTO antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/CRIPTO-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1598-img0010.jpg)
![Flow Cytometry: CRIPTO Antibody [NB100-1598] - An intracellular stain was performed on Jurkat with NB100-1598 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.](http://images.novusbio.com/fullsize/CRIPTO-Antibody-Flow-Cytometry-NB100-1598-img0012.jpg)
![Flow Cytometry: CRIPTO Antibody [NB100-1598] - An intracellular stain was performed on HeLa cells with NB100-1598PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin](http://images.novusbio.com/fullsize/CRIPTO-Antibody-Flow-Cytometry-NB100-1598-img0011.jpg)
![Knockout Validated: Vimentin Antibody (VM452) [NBP2-29433] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 0.7 ug/mL of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP2-29433) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Knockout-Validated-NBP2-29433-img0003.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: Vimentin Antibody (VM452) [NBP2-29433] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 0.7 ug/mL of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP2-29433) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody (VM452) [NBP2-29433] - Western Blot Analysis of human A375 cell lysate using Vimentin Antibody (VM452).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Western-Blot-NBP2-29433-img0008.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM452) [NBP2-29433] - Formalin-paraffin human melanoma stained with Vimentin Ab (VM452).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Immunohistochemistry-Paraffin-NBP2-29433-img0002.jpg)
![Flow Cytometry: Vimentin Antibody (VM452) [NBP2-29433] - Flow Cytometry of human Vimentin on Jurkat cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled Vimentin Antibody (VM452).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Flow-Cytometry-NBP2-29433-img0004.jpg)
![Western Blot: Vimentin Antibody (VM452) [NBP2-29433] - Western Blot Analysis Raji cell lysate Vimentin Antibody (VM452).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Western-Blot-NBP2-29433-img0007.jpg)
![Protein Array: Vimentin Antibody (VM452) [NBP2-29433] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Vimentin Antibody (VM452). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Protein-Array-NBP2-29433-img0006.jpg)
![Western Blot: Neprilysin/CD10 Antibody [NBP2-15771] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD10 antibody [N2C1], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-Western-Blot-NBP2-15771-img0015.jpg "Western Blot: Neprilysin/CD10 Antibody [NBP2-15771] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD10 antibody [N2C1], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: Neprilysin/CD10 Antibody [NBP2-15771] - A. 50 ug rat kidney lysate/extract 7.5 % SDS-PAGECD10 antibody [N2C1], Internal dilution: 1:500](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-Western-Blot-NBP2-15771-img0007.jpg)
![Western Blot: Neprilysin/CD10 Antibody [NBP2-15771] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD10 antibody [N2C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-Western-Blot-NBP2-15771-img0013.jpg)