| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05030-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93612-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03221-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89196-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92790-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48397 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59933-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24858SS | |||
![Western Blot: KIF3A Antibody [NBP3-05030] - Analysis of extracts of various cell lines, using KIF3A antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/fullsize/KIF3A-Antibody-Western-Blot-NBP3-05030-img0003.jpg "Western Blot: KIF3A Antibody [NBP3-05030] - Analysis of extracts of various cell lines, using KIF3A antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit")
![Immunocytochemistry/Immunofluorescence: KIF3A Antibody [NBP3-05030] - Analysis of MCF7 cells using KIF3A antibody . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/KIF3A-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05030-img0001.jpg)
![Immunoprecipitation: KIF3A Antibody [NBP3-05030] - Analysis of 200ug extracts of MCF-7 cells, using KIF3A antibody . Western blot was performed from the immunoprecipitate using this antibody.](http://images.novusbio.com/fullsize/KIF3A-Antibody-Immunoprecipitation-NBP3-05030-img0002.jpg)
![Immunohistochemistry-Paraffin: KIF3A Antibody [NBP3-05030] - Rat testis using KIF3A Rabbit pAb at dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/antibody/nbp3-05030_rabbit-polyclonal-kif3a-antibody-immunohistochemistry-paraffin-146202493146..jpg)
![Western Blot: WISP2 Antibody [NBP2-93612] - Analysis of extracts of Mouse ovary, using WISP2 antibody (NBP2-93612) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/WISP2 Antibody-Western Blot-NBP2-93612-img0003.jpg "Western Blot: WISP2 Antibody [NBP2-93612] - Analysis of extracts of Mouse ovary, using WISP2 antibody (NBP2-93612) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunohistochemistry-Paraffin: WISP2 Antibody [NBP2-93612] - Rat testis using WISP2 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/WISP2-Antibody-Immunohistochemistry-Paraffin-NBP2-93612-img0002.jpg)
![Immunohistochemistry-Paraffin: WISP2 Antibody [NBP2-93612] - Human colon using WISP2 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/WISP2-Antibody-Immunohistochemistry-Paraffin-NBP2-93612-img0001.jpg)
![ICC/IF-WISP2 Antibody [NBP2-93612] - HeLa cells using WISP2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/antibody/nbp2-93612_rabbit-polyclonal-wisp2-antibody-immunocytochemistry-immunofluorescence-301202474821..jpg)
![ICC/IF-WISP2 Antibody [NBP2-93612] - MCF7 cells using WISP2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/antibody/nbp2-93612_rabbit-polyclonal-wisp2-antibody-immunocytochemistry-immunofluorescence-301202474942..jpg)
![Western Blot: Vimentin Antibody [NBP3-03221] - Western blot analysis of extracts from normal (control) and Vimentin knockout (KO) 293T cells, using Vimentin antibody (NBP3-03221) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Western-Blot-NBP3-03221-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody [NBP3-03221] - Western blot analysis of extracts from normal (control) and Vimentin knockout (KO) 293T cells, using Vimentin antibody (NBP3-03221) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Vimentin Antibody [NBP3-03221] - Immunohistochemistry of paraffin-embedded human colon using [KO Validated] Vimentin Rabbit pAb (NBP3-03221) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP3-03221-img0002.jpg)
![Western Blot: Vimentin Antibody [NBP3-03221] - Western blot analysis of extracts of various cell lines, using Vimentin antibody (NBP3-03221) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Vimentin Antibody-Western Blot-NBP3-03221-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: Vimentin Antibody - Azide and BSA Free [Vimentin] - Immunofluorescence analysis of U2OS cells using [KD Validated] Vimentin Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03221_rabbit-polyclonal-vimentin-antibody-5220251628096.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (017) [NBP2-89196] - Staining of human SOX2 in human brain with rabbit monoclonal antibody (1:300).](http://images.novusbio.com/fullsize/SOX2-Antibody-017-Immunohistochemistry-Paraffin-NBP2-89196-img0002.jpg "Immunohistochemistry-Paraffin: SOX2 Antibody (017) [NBP2-89196] - Staining of human SOX2 in human brain with rabbit monoclonal antibody (1:300).")
![Immunohistochemistry-Paraffin: SOX2 Antibody (017) [NBP2-89196] - Staining of human SOX2 in human testis with rabbit monoclonal antibody (1:300).](http://images.novusbio.com/fullsize/SOX2-Antibody-017-Immunohistochemistry-Paraffin-NBP2-89196-img0001.jpg)
![Immunohistochemistry-Paraffin: COL1A2 Antibody [NBP2-92790] - Human placenta using Collagen I/COL1A2 Rabbit pAb (A5786) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/COL1A2-Antibody-Immunohistochemistry-Paraffin-NBP2-92790-img0004.jpg "Immunohistochemistry-Paraffin: COL1A2 Antibody [NBP2-92790] - Human placenta using Collagen I/COL1A2 Rabbit pAb (A5786) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.")
![Western Blot: COL1A2 Antibody [NBP2-92790] - Analysis of various lysates, using Collagen I/COL1A2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Negative control (NC):JurkatExposure time: 30s.](http://images.novusbio.com/fullsize/nbp2-92790_rabbit-polyclonal-col1a2-antibody-western-blot-1010202411939..jpg)
![Immunocytochemistry/ Immunofluorescence: COL1A2 Antibody - Azide and BSA Free [NBP2-92790] - Immunofluorescence analysis of A-431 cells using COL1A2 Rabbit pAb at dilution of 1:25. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92790_rabbit-polyclonal-col1a2-antibody-4220251429039.jpg)
![Immunocytochemistry/ Immunofluorescence: COL1A2 Antibody - Azide and BSA Free [NBP2-92790] - Immunofluorescence analysis of NIH/3T3 cells using COL1A2 Rabbit pAb at dilution of 1:25. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92790_rabbit-polyclonal-col1a2-antibody-42202514275255.jpg)
![Western Blot: HHATL Antibody [NBP2-81952] - Analysis of HHATL in 3T3 cell lysate with HHATL antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/HHATL-Antibody-Western-Blot-NBP2-81952-img0001.jpg "Western Blot: HHATL Antibody [NBP2-81952] - Analysis of HHATL in 3T3 cell lysate with HHATL antibody at 1 ug/ml.")
![Immunohistochemistry: HHATL Antibody [NBP2-81952] - Analysis of HHATL in human skin tissue with HHATL antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/HHATL-Antibody-Immunohistochemistry-NBP2-81952-img0002.jpg)
![Immunohistochemistry: HHATL Antibody - BSA Free [NBP2-81952] - Immunohistochemistry of HHATL in human skin tissue with HHATL antibody at 2.5 u/mL.](http://images.novusbio.com/fullsize/nbp2-81952_rabbit-polyclonal-hhatl-antibody-2711202419451220.jpg)
![Immunocytochemistry/ Immunofluorescence: HHATL Antibody - BSA Free [NBP2-81952] - Immunofluorescence of HHATL in human skin tissue with HHATL antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp2-81952_rabbit-polyclonal-hhatl-antibody-2711202419475856.jpg)
![Western Blot: HHATL Antibody - BSA Free [NBP2-81952] - Western blot analysis of HHATL in 3T3 cell lysate with HHATL antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/nbp2-81952_rabbit-polyclonal-hhatl-antibody-2711202419172281.gif)
![Immunocytochemistry/Immunofluorescence: Glypican 3 Antibody (SPM595) - Azide and BSA Free [NBP2-47761] - Immunofluorescence Analysis of MeOH-fixed HepG2 cells labeling Glypican-3 with Glypican 3 Antibody (SPM595) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/Glypican-3-Antibody-SPM595-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-47761-img0004.jpg "Immunocytochemistry/Immunofluorescence: Glypican 3 Antibody (SPM595) - Azide and BSA Free [NBP2-47761] - Immunofluorescence Analysis of MeOH-fixed HepG2 cells labeling Glypican-3 with Glypican 3 Antibody (SPM595) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)")
![Immunohistochemistry-Paraffin: Glypican 3 Antibody (SPM595) - Azide and BSA Free [NBP2-47761] - Human Melanoma stained with Glypican-3 Monoclonal Antibody (SPM595)](http://images.novusbio.com/fullsize/Glypican-3-Antibody-SPM595-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47761-img0003.jpg)
![Flow Cytometry: Glypican 3 Antibody (SPM595) - Azide and BSA Free [NBP2-47761] - Flow Cytometric Analysis of PFA-fixed HepG2 cells using Glypican 3 Antibody (SPM595) followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Glypican-3-Antibody-SPM595-Azide-and-BSA-Free-Flow-Cytometry-NBP2-47761-img0001.jpg)
![Immunohistochemistry-Paraffin: Glypican 3 Antibody (SPM595) - Azide and BSA Free [NBP2-47761] - Human Hepatocellular Carcinoma stained with Glypican-3 Monoclonal Antibody (SPM595)](http://images.novusbio.com/fullsize/Glypican-3-Antibody-SPM595-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47761-img0002.jpg)
![Western Blot: PIWIL2 Antibody [NBP2-24590] - Analysis of Mili in mouse embryo testis lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/PIWIL2-Antibody-Western-Blot-NBP2-24590-img0002.jpg "Western Blot: PIWIL2 Antibody [NBP2-24590] - Analysis of Mili in mouse embryo testis lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: PIWIL2 Antibody [NBP2-24590] - Immunohistochemical analysis of PIWIL2 in formalin-fixed, paraffin-embedded mouse testis tissue using an isotype control (top left) and NBP2-24590 (bottom left, right) at 5 ug/ml.](http://images.novusbio.com/fullsize/PIWIL2-Antibody-Immunohistochemistry-Paraffin-NBP2-24590-img0001.jpg)
![Western Blot: Lamin A + C Antibody (WL4G10) [NBP2-59933] - Lamin A + C (WL4G10) [NBP2-59933] - Whole cell lysates from HeLa and 293T cells were separated on SDS-PAGE gel, transferred to nitrocellulose membrane and stained with WL4G10 antibody. The membrane was blocked overnight in blocking buffer (5% nonfat dry milk resuspended in 0.1% Tween and 1X PBS). Both primary and secondary antibodies were diluted in blocking buffer. As expected, 293T cells express less of the lamin A isoform.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-WL4G10-Western-Blot-NBP2-59933-img0006.jpg "Western Blot: Lamin A + C Antibody (WL4G10) [NBP2-59933] - Lamin A + C (WL4G10) [NBP2-59933] - Whole cell lysates from HeLa and 293T cells were separated on SDS-PAGE gel, transferred to nitrocellulose membrane and stained with WL4G10 antibody. The membrane was blocked overnight in blocking buffer (5% nonfat dry milk resuspended in 0.1% Tween and 1X PBS). Both primary and secondary antibodies were diluted in blocking buffer. As expected, 293T cells express less of the lamin A isoform.")
![Immunohistochemistry-Paraffin: Lamin A + C Antibody (WL4G10) [NBP2-59933] - Analysis of formalin-fixed paraffin-embedded human gut. Following heat induced antigen retrieval, the sections were stained with mouse monoclonal anti-lamin A+C clone WL4G10 antibody (green). Nuclei were visualized with DAPI.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-WL4G10-Immunocytochemistry-Immunofluorescence-NBP2-59933-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (WL4G10) [NBP2-59933] - Confocal images of methanol fixed Hela cells that were immunofluorescently stained with mouse monoclonal WL4G10 antibody. The antibody works as well on cells fixed with 4% PFA.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-WL4G10-Immunocytochemistry-Immunofluorescence-NBP2-59933-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (WL4G10) [NBP2-59933] - The mouse monoclonal WL4G10 antibody recognizes human lamins A and C. Immortalized mouse adult LMNA null fibroblast stably expressing: (A) mEos2-lamin A or (B) mEos2- lamin C fused were fixed in 4% PFA and stained with WL4G10 antibody (magenta). Expression of the respective LMNA isoform is shown in green. The nuclei were counter-stained with Hoechst. Of note, the WL4G10 antibody does not recognize mouse lamins A and C.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-WL4G10-Immunocytochemistry-Immunofluorescence-NBP2-59933-img0003.jpg)
![Western Blot: IL-33 Antibody [NBP2-24858] - Analysis of IL-33 using IL-33 polyclonal antibody. HUVEC cell lysate probed with IL-33 antibody at 1 ug/ml in the 1) absence and 2) presence of immunizing peptide.](http://images.novusbio.com/fullsize/IL-33-Antibody-Western-Blot-NBP2-24858-img0003.jpg "Western Blot: IL-33 Antibody [NBP2-24858] - Analysis of IL-33 using IL-33 polyclonal antibody. HUVEC cell lysate probed with IL-33 antibody at 1 ug/ml in the 1) absence and 2) presence of immunizing peptide.")
![Simple Western: IL-33 Antibody [NBP2-24858] - Lane view shows a specific band for IL-33 in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/IL-33-Antibody-Simple-Western-NBP2-24858-img0002.jpg)
![Western Blot: GATA-2 Antibody (2D11) [H00002624-M01] - Analysis of GATA2 expression in transfected 293T cell line by GATA2 monoclonal antibody (M01), clone 2D11.Lane 1: GATA2 transfected lysate(50.5 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/GATA-2-Antibody-2D11-Western-Blot-H00002624-M01-img0016.jpg "Western Blot: GATA-2 Antibody (2D11) [H00002624-M01] - Analysis of GATA2 expression in transfected 293T cell line by GATA2 monoclonal antibody (M01), clone 2D11.Lane 1: GATA2 transfected lysate(50.5 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: GATA-2 Antibody (2D11) [H00002624-M01] - Analysis of monoclonal antibody to GATA2 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/GATA-2-Antibody-2D11-Immunocytochemistry-Immunofluorescence-H00002624-M01-img0012.jpg)
![Western Blot: GATA-2 Antibody (2D11) [H00002624-M01] - Analysis of GATA2 over-expressed 293 cell line, cotransfected with GATA2 Validated Chimera RNAi ( Cat # H00002624-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with GATA2 monoclonal antibody (M01), clone 2D11 (Cat # H00002624-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/GATA-2-Antibody-2D11-Western-Blot-H00002624-M01-img0013.jpg)
![Western Blot: GATA-2 Antibody (2D11) [H00002624-M01] - GATA2 monoclonal antibody (M01), clone 2D11 Analysis of GATA2 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/GATA-2-Antibody-2D11-Western-Blot-H00002624-M01-img0015.jpg)
![ELISA: GATA-2 Antibody (2D11) [H00002624-M01] - Detection limit for recombinant GST tagged GATA2 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/GATA-2-Antibody-2D11-ELISA-H00002624-M01-img0017.jpg)
![Chromatin Immunoprecipitation: SOX2 Antibody (PO00-28) [NBP3-32972] - Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either SOX2 (NBP3-32972) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.](http://images.novusbio.com/fullsize/nbp3-32972_rabbit-sox2-mab-po00-28-26820241525104.jpg "Chromatin Immunoprecipitation: SOX2 Antibody (PO00-28) [NBP3-32972] - Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either SOX2 (NBP3-32972) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.")
![Western Blot: SOX2 Antibody (PO00-28) [NBP3-32972] - Western blot analysis of SOX2 on different lysates with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/2,000 dilution. Lane 1: NCCIT cell lysate Lane 2: F9 cell lysate Lane 3: HeLa cell lysate (negative) Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32972) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32972_rabbit-sox2-mab-po00-28-28820241249344.jpg)
![Immunohistochemistry: SOX2 Antibody (PO00-28) [NBP3-32972] - Immunohistochemical analysis of paraffin-embedded human trachea tissue with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32972) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32972_rabbit-sox2-mab-po00-28-298202414161844.jpg)
![Immunocytochemistry/ Immunofluorescence: SOX2 Antibody (PO00-28) [NBP3-32972] - Immunocytochemistry analysis of F9 cells labeling SOX2 with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32972_rabbit-sox2-mab-po00-28-29202410334048.jpg)