| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37483-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-50056-20ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27073SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54593-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-59619 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77458SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57554-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44099 | |||
| BSA Free | NBP2-59057-25ul | |||
![Western Blot: Glypican 3 Antibody (9C2) [NBP2-37483] - Analysis using GPC3 mouse mAb against HepG2 (1), HEK293 (2), Jurkat (3), SK-N-SH (4), PC-12 (5), F9 (6)and Mouse liver (7) cell lysate.](http://images.novusbio.com/fullsize/Glypican-3-Antibody-9C2-Western-Blot-NBP2-37483-img0003.jpg "Western Blot: Glypican 3 Antibody (9C2) [NBP2-37483] - Analysis using GPC3 mouse mAb against HepG2 (1), HEK293 (2), Jurkat (3), SK-N-SH (4), PC-12 (5), F9 (6)and Mouse liver (7) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Glypican 3 Antibody (9C2) [NBP2-37483] - Analysis of HeLa cells using GPC3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Glypican-3-Antibody-9C2-Immunocytochemistry-Immunofluorescence-NBP2-37483-img0004.jpg)
![Immunohistochemistry-Paraffin: Glypican 3 Antibody (9C2) [NBP2-37483] - Analysis of breast cancer tissues using GPC3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Glypican-3-Antibody-9C2-Immunohistochemistry-NBP2-37483-img0007.jpg)
![Flow Cytometry: Glypican 3 Antibody (9C2) [NBP2-37483] - Analysis of Jurkat cells using GPC3 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Glypican-3-Antibody-9C2-Flow-Cytometry-NBP2-37483-img0005.jpg)
![Western Blot: Glypican 3 Antibody (9C2) [NBP2-37483] - Analysis using GPC3 mAb against human GPC3 (AA: 55-200) recombinant protein. (Expected MW is 28.5 kDa)](http://images.novusbio.com/fullsize/Glypican-3-Antibody-9C2-Western-Blot-NBP2-37483-img0002.jpg)
![Immunohistochemistry-Paraffin: Glypican 3 Antibody (9C2) [NBP2-37483] - Analysis of liver cancer tissues using GPC3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Glypican-3-Antibody-9C2-Immunohistochemistry-NBP2-37483-img0006.jpg)
![ELISA: Glypican 3 Antibody (9C2) [NBP2-37483] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Glypican-3-Antibody-9C2-ELISA-NBP2-37483-img0001.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Staining of CD16 in human peripheral blood with anti-CD16 (3G8) APC.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0014.jpg "Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Staining of CD16 in human peripheral blood with anti-CD16 (3G8) APC.")
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) APC-CyTM7.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0001.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) PE.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0002.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) PE-CyTM7.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0003.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) PerCP.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0004.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) PerC-CyTM5.5](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0005.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) Pacific BlueTM.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0006.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) purified / GAM-APC.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0008.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Staining of CD16 in human peripheral blood with anti-CD16 (3G8) Alexa Fluor® 647.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0009.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Staining of CD16 in human peripheral blood with anti-CD16 (3G8) Pacific OrangeTM.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0010.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Staining of CD16 in human peripheral blood with anti-CD16 (3G8) biotin.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0011.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Staining of human peripheral blood with anti-CD16 (3G8) FITC.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0012.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (3G8) [NBP1-50056] - Staining of CD16 in human peripheral blood with anti-CD16 (3G8) Alexa Fluor® 488.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-3G8-Flow-Cytometry-NBP1-50056-img0013.jpg)
![Western Blot: Vang-like Protein 2/VANGL2 Antibody [NBP2-24704] - Analysis of Van Gogh like-2 in human brain lysate in the 1) absence and2) presence of immunizing peptide, 3) mouse brain and 4) rat brain using this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Vang-like-Protein-2-VANGL2-Antibody-Western-Blot-NBP2-24704-img0006.jpg "Western Blot: Vang-like Protein 2/VANGL2 Antibody [NBP2-24704] - Analysis of Van Gogh like-2 in human brain lysate in the 1) absence and2) presence of immunizing peptide, 3) mouse brain and 4) rat brain using this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: Vang-like Protein 2/VANGL2 Antibody [NBP2-24704] - Analysis of Van Gogh like-2 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.](http://images.novusbio.com/fullsize/Vang-like-Protein-2-VANGL2-Antibody-Immunohistochemistry-Paraffin-NBP2-24704-img0005.jpg)
![Western Blot: HOXB4 Antibody [NBP2-24561] - Analysis of HOXB4 in K562 cell lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/HOXB4-Antibody-Western-Blot-NBP2-24561-img0003.jpg "Western Blot: HOXB4 Antibody [NBP2-24561] - Analysis of HOXB4 in K562 cell lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: HOXB4 Antibody [NBP2-24561] - Analysis of HOXB4 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/ml.](http://images.novusbio.com/fullsize/HOXB4-Antibody-Immunohistochemistry-Paraffin-NBP2-24561-img0001.jpg)
![Western Blot: FABP7/B-FABP Antibody [NBP2-27073] - Analysis of FABP7 in human spleen in the 1) absence and 2) presence of immunizing peptide, 3) mouse spleen, and 4) mouse embryonic brain lysate using this antibody. goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Western-Blot-NBP2-27073-img0004.jpg "Western Blot: FABP7/B-FABP Antibody [NBP2-27073] - Analysis of FABP7 in human spleen in the 1) absence and 2) presence of immunizing peptide, 3) mouse spleen, and 4) mouse embryonic brain lysate using this antibody. goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: FABP7/B-FABP Antibody [NBP2-27073] - Analysis of FABP7 in formalin-fixed, paraffin-embedded human brain tissue using this antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/FABP7-B-FABP-Antibody-Immunohistochemistry-Paraffin-NBP2-27073-img0003.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1792) [NBP2-53408] - Formalin-fixed, paraffin-embedded Mouse Intestine stained with SOX2 Antibody (SOX2/1792).](http://images.novusbio.com/fullsize/SOX2-Antibody-SOX2-1792-Immunohistochemistry-Paraffin-NBP2-53408-img0005.jpg "Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1792) [NBP2-53408] - Formalin-fixed, paraffin-embedded Mouse Intestine stained with SOX2 Antibody (SOX2/1792).")
![Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1792) [NBP2-53408] - Formalin-fixed, paraffin-embedded Human Cervical Carcinoma stained with SOX2 Monoclonal Antibody (SOX2/1792).](http://images.novusbio.com/fullsize/SOX2-Antibody-SOX2-1792-Immunohistochemistry-Paraffin-NBP2-53408-img0001.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1792) [NBP2-53408] - Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with SOX2 Antibody (SOX2/1792).](http://images.novusbio.com/fullsize/SOX2-Antibody-SOX2-1792-Immunohistochemistry-Paraffin-NBP2-53408-img0003.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1792) [NBP2-53408] - Formalin-fixed, paraffin-embedded Mouse Stomach stained with SOX2 Antibody (SOX2/1792).](http://images.novusbio.com/fullsize/SOX2-Antibody-SOX2-1792-Immunohistochemistry-Paraffin-NBP2-53408-img0004.jpg)
![Protein Array: SOX2 Antibody (SOX2/1792) [NBP2-53408] - Analysis of Protein Array containing more than 19,000 full-length human proteins using SOX2 Antibody (SOX2/1792) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD?s) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD?s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/SOX2-Antibody-SOX2-1792-Protein-Array-NBP2-53408-img0006.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1792) [NBP2-53408] - Analysis of SOX2 stained human head-neck cancer sample. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-53408_mouse-monoclonal-sox2-antibody-sox2-1792-17720241044646.jpg)
![Western Blot: SOX2 Antibody (SOX2/1792) [NBP2-53408] - Western Blot of Recombinant SOX2 protein fragment and NCCIT cell lysate using SOX2 Antibody (SOX2/1792).](http://images.novusbio.com/fullsize/nbp2-53408_mouse-monoclonal-sox2-antibody-sox2-1792-17720241003232.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (S100B/1012) [NBP2-59618] - Confocal Immunofluorescent analysis of A2058 cells using AF488-labeled (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue). (Negative Control)](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1012-Immunocytochemistry-Immunofluorescence-NBP2-59618-img0007.jpg "Immunocytochemistry/Immunofluorescence: S100B Antibody (S100B/1012) [NBP2-59618] - Confocal Immunofluorescent analysis of A2058 cells using AF488-labeled (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue). (Negative Control)")
![Protein Array: S100B Antibody (S100B/1012) [NBP2-59618] - containing more than 19,000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary) produces when binding to a particular protein on the HuProt array. Z-scores are described in units of standard deviations above the mean value of all signals generated on that array. If targets on HuProt are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of an Ab to its intended target. An Ab is considered to specific to its intended target, if the Ab has an S-score of at least 2.5. For example, if Ab binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Ab to protein X is equal to 29](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1012-Protein-Array-NBP2-59618-img0001.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (S100B/1012) [NBP2-59618] - Formalin-fixed. Paraffin-embedded human Melanoma stained with S100B Mouse Monoclonal Antibody (S100B/1012).](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1012-Immunohistochemistry-Paraffin-NBP2-59618-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (S100B/1012) [NBP2-59618] - Confocal Immunofluorescent analysis of A2058 cells using AF488-labeled S100B Antibody (S100B/1012) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1012-Immunocytochemistry-Immunofluorescence-NBP2-59618-img0006.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (S100B/1012) [NBP2-59618] - Formalin-fixed, paraffin-embedded human Schwanoma stained with S100B Mouse Monoclonal Antibody (S100B/1012).](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1012-Immunohistochemistry-Paraffin-NBP2-59618-img0004.jpg)
![Immunohistochemistry: Collagen I alpha 1 Antibody [NBP1-77458] - Analysis of Collagen 1 in mouse epidermis using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Collagen-I-Antibody-Immunohistochemistry-NBP1-77458-img0003.jpg "Immunohistochemistry: Collagen I alpha 1 Antibody [NBP1-77458] - Analysis of Collagen 1 in mouse epidermis using DAB with hematoxylin counterstain.")
![Immunocytochemistry/Immunofluorescence: Collagen I alpha 1 Antibody [NBP1-77458] - Collagen 1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77458-img0004.jpg)
![Western Blot: NeuroD1 Antibody (3H8) [H00004760-M01] - Analysis of NEUROD1 expression in transfected 293T cell line by NEUROD1 monoclonal antibody (M01), clone 3H8.Lane 1: NEUROD1 transfected lysate(39.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NeuroD1-Antibody-3H8-Western-Blot-H00004760-M01-img0011.jpg "Western Blot: NeuroD1 Antibody (3H8) [H00004760-M01] - Analysis of NEUROD1 expression in transfected 293T cell line by NEUROD1 monoclonal antibody (M01), clone 3H8.Lane 1: NEUROD1 transfected lysate(39.9 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: NeuroD1 Antibody (3H8) [H00004760-M01] - Analysis of monoclonal antibody to NEUROD1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/NeuroD1-Antibody-3H8-Immunocytochemistry-Immunofluorescence-H00004760-M01-img0007.jpg)
![Immunohistochemistry-Paraffin: NeuroD1 Antibody (3H8) [H00004760-M01] - Analysis of monoclonal antibody to NEUROD1 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/NeuroD1-Antibody-3H8-Immunohistochemistry-Paraffin-H00004760-M01-img0009.jpg)
![Western Blot: NeuroD1 Antibody (3H8) [H00004760-M01] - Western Blot analysis of NEUROD1 expression in IMR-32 ( Cat # L008V1 ).](http://images.novusbio.com/fullsize/NeuroD1-Antibody-3H8-Western-Blot-H00004760-M01-img0001.jpg)
![ELISA: NeuroD1 Antibody (3H8) [H00004760-M01] - Detection limit for recombinant GST tagged NEUROD1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NeuroD1-Antibody-3H8-ELISA-H00004760-M01-img0012.jpg)
![Western Blot: SMURF2 Antibody [NBP2-57554] - Analysis in human cell line U-251 MG.](http://images.novusbio.com/fullsize/SMURF2-Antibody-Western-Blot-NBP2-57554-img0008.jpg "Western Blot: SMURF2 Antibody [NBP2-57554] - Analysis in human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: SMURF2 Antibody [NBP2-57554] - Staining of human cell line BJ shows localization to nuclear speckles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SMURF2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57554-img0001.jpg)
![Immunohistochemistry-Paraffin: SMURF2 Antibody [NBP2-57554] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/SMURF2-Antibody-Immunohistochemistry-Paraffin-NBP2-57554-img0007.jpg)
![Immunohistochemistry-Paraffin: SMURF2 Antibody [NBP2-57554] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/SMURF2-Antibody-Immunohistochemistry-Paraffin-NBP2-57554-img0004.jpg)
![Immunohistochemistry-Paraffin: SMURF2 Antibody [NBP2-57554] - Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/SMURF2-Antibody-Immunohistochemistry-Paraffin-NBP2-57554-img0005.jpg)
![Immunohistochemistry-Paraffin: SMURF2 Antibody [NBP2-57554] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/SMURF2-Antibody-Immunohistochemistry-Paraffin-NBP2-57554-img0006.jpg)
![Immunohistochemistry: 5-hmC Antibody (RM236) [NBP2-61469] - Immunohistochemical staining of formalin fixed and paraffin embedded Human brain tissue sections, using NBP2-61469](http://images.novusbio.com/fullsize/5-hmC-Antibody-RM236-Immunohistochemistry-NBP2-61469-img0009.jpg "Immunohistochemistry: 5-hmC Antibody (RM236) [NBP2-61469] - Immunohistochemical staining of formalin fixed and paraffin embedded Human brain tissue sections, using NBP2-61469")
![Flow Cytometry: 5-hmC Antibody (RM236) [NBP2-61469] - Flow Cytometry analysis of 5-hmC expression in HEK293 cells using NBP2-61470. The cells were fixed with ice-cold MeOH, permeabilized with 0.5% Triton X-100, denatured with 2N HCl, then stained with NBP2-61469 (Blue) or with a negative control antibody (red)](http://images.novusbio.com/fullsize/5-hmC-Antibody-RM236-Flow-Cytometry-NBP2-61469-img0014.jpg)
![Methylated DNA Immunoprecipitation: 5-hmC Antibody (RM236) [NBP2-61469] - hMeDIP was performed using Anti-5-hmC antibody (NBP2-61469) at a 10:1 DNA: Ab ratio. 1 ng of unmethylated, 5-Methylcytosine (5-mC) or 5-Hydroxymethylcytosine (5-hmC) DNA standard (897 bp) was spiked in 1ug of genomic DNA isolated from HeLa cells as the control. Realtime PCR was then performed to determine the capture of DNA standard as in % of input.](http://images.novusbio.com/fullsize/nbp2-61469_rabbit-monoclonal-5-hmc-antibody-rm236-methylated-dna-immunoprecipitation-189202523512111.png)
![ELISA: 5-hmC Antibody (RM236) [NBP2-61469] - Direct ELISA of Mouse brain genomic DNA using Anti-5-hmC antibody (NBP2-61469). The plate was directly coated with different concentrations of genomic DNA isolated from Mouse brain tissue. NBP2-61469 was used as the primary antibody, and a HRP conjugated Anti-Rabbit IgG as the secondary antibody.](http://images.novusbio.com/fullsize/nbp2-61469_rabbit-monoclonal-5-hmc-antibody-rm236-elisa-189202523512124.png)
![ELISA: 5-hmC Antibody (RM236) [NBP2-61469] - ELISA of single stranded DNA using Anti-5-hmC antibody (NBP2-61469). The plate was coated with streptavidin and then biotinylated single stranded unmethylated DNA, 5-Methylcytosine (5-mC) DNA, and 5-Hydroxymethylcytosine (5-hmC) DNA. A serial dilution of NBP2-61469 was used as the primary antibody, and an alkaline phosphatase conjugated Anti-Rabbit IgG as the secondary antibody.](http://images.novusbio.com/fullsize/nbp2-61469_rabbit-monoclonal-5-hmc-antibody-rm236-elisa-189202523521116.png)
![Dot Blot: 5-hmC Antibody (RM236) [NBP2-61469] - Dot blot of double stranded DNA using NBP2-61469. The membrane was pre-spotted with double stranded 5-Hydroxymethylcytosine (5-hmC) DNA, 5-Methylcytosine (5-mC) DNA, and unmethylated DNA.](http://images.novusbio.com/fullsize/nbp2-61469_rabbit-monoclonal-5-hmc-antibody-rm236-dot-blot-18920252338175.png)
![Immunocytochemistry/Immunofluorescence: 5-hmC Antibody (RM236) [NBP2-61469] - Immunocytochemical staining of HeLa cells using NBP2-61469 at 0.5 ug/ml (red) . Actin filaments was labeled with fluorescein phalloidin green). HeLa cells were fixed with 4% paraformaldehyde and permeabilized with methanol (-20C) before treatment with 2 N HCl for 30 min at 37C to denature the DNA.](http://images.novusbio.com/fullsize/nbp2-61469_rabbit-monoclonal-5-hmc-antibody-rm236-immunocytochemistry-immunofluorescence-199202502430.png)
![Western Blot: SOX2 Antibody (CL4716) [NBP2-59057] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-SOX2 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/SOX2-Antibody-CL4716-Western-Blot-NBP2-59057-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: SOX2 Antibody (CL4716) [NBP2-59057] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-SOX2 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.")
![<b>Genetic Strategies Validation. </b>Western Blot: SOX2 Antibody (CL4716) [NBP2-59057] - Analysis in human cell line NTERA-2.](http://images.novusbio.com/fullsize/SOX2-Antibody-CL4716-Western-Blot-NBP2-59057-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: SOX2 Antibody (CL4716) [NBP2-59057] - Staining of U-251 cells using the anti-SOX2 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/SOX2-Antibody-CL4716-Immunocytochemistry-Immunofluorescence-NBP2-59057-img0001.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (CL4716) [NBP2-59057] - Staining of human placenta shows no positivity as expected (negative control).](http://images.novusbio.com/fullsize/SOX2-Antibody-CL4716-Immunohistochemistry-Paraffin-NBP2-59057-img0005.jpg)
![Immunohistochemistry: SOX2 Antibody (CL4716) [NBP2-59057] - SOX2 Antibody [NBP2-59057] - Staining of mouse embryo E11 shows nuclear immunoreactivity in the developing brain, eye and trigeminal ganglion.](http://images.novusbio.com/fullsize/SOX2-Antibody-CL4716-Immunohistochemistry-NBP2-59057-img0007.jpg)
![Immunohistochemistry: SOX2 Antibody (CL4716) [NBP2-59057] - SOX2 Antibody [NBP2-59057] - Staining of mouse embryo E11 shows nuclear immunoreactivity in the developing neural tube.](http://images.novusbio.com/fullsize/SOX2-Antibody-CL4716-Immunohistochemistry-NBP2-59057-img0008.jpg)