| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77102-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70374 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-65537-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-43346-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-65362-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74840 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-04320SS | |||
![Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody (TRA-2-49) - BSA Free [NB600-540] - Confocal fluorescent analysis of A431 using anti-TRA-2-49 Mouse monoclonal antibody (Red). Actin filaments have been labeled with AlexaFluor(R) 488-Phalloidin (Green). Nucleus is stained with DAPI (Blue). Positive cytosol staining.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-TRA-2-49-BSA-Free-Immunocytochemistry-Immunofluorescence-NB600-540-img0011.jpg)
![Flow Cytometry: Alkaline Phosphatase, Tissue Non-Specific Antibody (TRA-2-49) [NB600-540] - Analysis using anti-TRA-2-49. 2102Ep cells were stained with anti-TRA-2-49 antibody (Green) or isotype control antibody (grey).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-TRA-2-49-Flow-Cytometry-NB600-540-img0008.jpg)
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15336] - PG-4 whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A1 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-Western-Blot-NBP2-15336-img0021.jpg "Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15336] - PG-4 whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A1 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15336] - Analysis of methanol-fixed A549, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ALDH1A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15336-img0001.jpg)
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15336] - Detects ALDH1A1 protein by Western blot analysis. A. 50 ug rat liver lysate/extract 10 % SDS-PAGE Dilution: 1:5000](http://images.novusbio.com/fullsize/ALDH1A1-Antibody-Western-Blot-NBP2-15336-img0012.jpg)
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15336] - Detects ALDH1A1 protein by Western blot analysis. A. 50 ug mouse liver lysate/extract 10 % SDS-PAGE Dilution: 1:5000](http://images.novusbio.com/fullsize/ALDH1A1-Antibody-Western-Blot-NBP2-15336-img0015.jpg)
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15336] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A1 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-Western-Blot-NBP2-15336-img0018.jpg)
![Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15336] - Human gastric cancer, using ALDH1A1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ALDH1A1-Antibody-Immunohistochemistry-Paraffin-NBP2-15336-img0002.jpg)
![Western Blot: Synaptogyrin 3 Antibody [NBP1-77102] - Mouse brain tissue lysate at (A) 1 and (B) 2 ug/mL .](http://images.novusbio.com/fullsize/Synaptogyrin-3-Antibody-Western-Blot-NBP1-77102-img0003.jpg "Western Blot: Synaptogyrin 3 Antibody [NBP1-77102] - Mouse brain tissue lysate at (A) 1 and (B) 2 ug/mL .")
![Immunohistochemistry-Paraffin: Synaptogyrin 3 Antibody [NBP1-77102] - Rat brain tissue with SYNGR3 antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/Synaptogyrin-3-Antibody-Immunohistochemistry-Paraffin-NBP1-77102-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Synaptogyrin 3 Antibody - BSA Free [NBP1-77102] - Immunofluorescence of Synaptogyrin 3 in Rat Brain cells with Synaptogyrin 3 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77102_rabbit-polyclonal-synaptogyrin-3-antibody-271120242045676.jpg)
![Western Blot: PLK1 Antibody (13E8) [NB100-74502] - Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with PLK1 mouse monoclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed.](http://images.novusbio.com/fullsize/PLK1-Antibody-13E8-Western-Blot-NB100-74502-img0004.jpg "Western Blot: PLK1 Antibody (13E8) [NB100-74502] - Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with PLK1 mouse monoclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed.")
![Immunocytochemistry/Immunofluorescence: PLK1 Antibody (13E8) [NB100-74502] - PLK1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) in WiDr colon carcinoma cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PLK1 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/PLK1-Antibody-13E8-Immunocytochemistry-Immunofluorescence-NB100-74502-img0010.jpg)
![Immunohistochemistry-Paraffin: PLK1 Antibody (13E8) [NB100-74502] - Both normal and cancer biopsies of deparaffinized human Colon tissue.](http://images.novusbio.com/fullsize/PLK1-Antibody-13E8-Immunohistochemistry-Paraffin-NB100-74502-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: PLK1 Antibody (13E8) [NB100-74502] - PLK1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PLK1 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/PLK1-Antibody-13E8-Immunocytochemistry-Immunofluorescence-NB100-74502-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: PLK1 Antibody (13E8) [NB100-74502] - PLK1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) in U251 glioma cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PLK1 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/PLK1-Antibody-13E8-Immunocytochemistry-Immunofluorescence-NB100-74502-img0009.jpg)
![Western Blot: CD30/TNFRSF8 Antibody (1C6) [NBP2-46157] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNFRSF8.](http://images.novusbio.com/fullsize/CD30-TNFRSF8-Antibody-1C6-Western-Blot-NBP2-46157-img0021.jpg "Western Blot: CD30/TNFRSF8 Antibody (1C6) [NBP2-46157] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNFRSF8.")
![Immunohistochemistry: CD30/TNFRSF8 Antibody (1C6) [NBP2-46157] - Analysis of Human endometrium tissue.](http://images.novusbio.com/fullsize/CD30-TNFRSF8-Antibody-1C6-Immunohistochemistry-NBP2-46157-img0020.jpg)
![Flow Cytometry: CD30/TNFRSF8 Antibody (1C6) [NBP2-46157] - (Blue).(1:100)](http://images.novusbio.com/fullsize/CD30-TNFRSF8-Antibody-1C6-Flow-Cytometry-NBP2-46157-img0022.jpg)
![Immunohistochemistry: CD30/TNFRSF8 Antibody (1C6) [NBP2-46157] - Analysis of Human lymphoma tissue.](http://images.novusbio.com/fullsize/CD30-TNFRSF8-Antibody-1C6-Immunohistochemistry-NBP2-46157-img0001.jpg)
![Immunohistochemistry: CD30/TNFRSF8 Antibody (1C6) [NBP2-46157] - Analysis of Carcinoma of Human kidney tissue.](http://images.novusbio.com/fullsize/CD30-TNFRSF8-Antibody-1C6-Immunohistochemistry-NBP2-46157-img0002.jpg)
![Immunohistochemistry: CD30/TNFRSF8 Antibody (1C6) [NBP2-46157] - Analysis of Human lung tissue.](http://images.novusbio.com/fullsize/CD30-TNFRSF8-Antibody-1C6-Immunohistochemistry-NBP2-46157-img0003.jpg)
![Immunohistochemistry: CD30/TNFRSF8 Antibody (1C6) [NBP2-46157] - Analysis of Adenocarcinoma of Human ovary tissue.](http://images.novusbio.com/fullsize/CD30-TNFRSF8-Antibody-1C6-Immunohistochemistry-NBP2-46157-img0004.jpg)
![Immunohistochemistry: CD30/TNFRSF8 Antibody (1C6) [NBP2-46157] - Analysis of Carcinoma of Human bladder tissue.](http://images.novusbio.com/fullsize/CD30-TNFRSF8-Antibody-1C6-Immunohistochemistry-NBP2-46157-img0005.jpg)
![Immunohistochemistry: CD30/TNFRSF8 Antibody (1C6) [NBP2-46157] - Analysis of Human prostate tissue.](http://images.novusbio.com/fullsize/CD30-TNFRSF8-Antibody-1C6-Immunohistochemistry-NBP2-46157-img0006.jpg)
![Immunohistochemistry: CD30/TNFRSF8 Antibody (1C6) [NBP2-46157] - Analysis of Human bladder tissue..](http://images.novusbio.com/fullsize/CD30-TNFRSF8-Antibody-1C6-Immunohistochemistry-NBP2-46157-img0007.jpg)
![Immunohistochemistry: CD30/TNFRSF8 Antibody (1C6) [NBP2-46157] - Analysis of Human Kidney tissue.](http://images.novusbio.com/fullsize/CD30-TNFRSF8-Antibody-1C6-Immunohistochemistry-NBP2-46157-img0008.jpg)
![Immunohistochemistry: CD30/TNFRSF8 Antibody (1C6) [NBP2-46157] - Analysis of Adenocarcinoma of Human breast tissue.](http://images.novusbio.com/fullsize/CD30-TNFRSF8-Antibody-1C6-Immunohistochemistry-NBP2-46157-img0009.jpg)
![Immunohistochemistry: CD30/TNFRSF8 Antibody (1C6) [NBP2-46157] - Analysis of Carcinoma of Human liver tissue.](http://images.novusbio.com/fullsize/CD30-TNFRSF8-Antibody-1C6-Immunohistochemistry-NBP2-46157-img0010.jpg)
![Immunohistochemistry: CD30/TNFRSF8 Antibody (1C6) [NBP2-46157] - Analysis of Human Ovary tissue.](http://images.novusbio.com/fullsize/CD30-TNFRSF8-Antibody-1C6-Immunohistochemistry-NBP2-46157-img0011.jpg)
![Immunohistochemistry: CD30/TNFRSF8 Antibody (1C6) [NBP2-46157] - Analysis of Human pancreas tissue.](http://images.novusbio.com/fullsize/CD30-TNFRSF8-Antibody-1C6-Immunohistochemistry-NBP2-46157-img0012.jpg)
![Immunohistochemistry: CD30/TNFRSF8 Antibody (1C6) [NBP2-46157] - Analysis of Carcinoma of Human thyroid tissue.](http://images.novusbio.com/fullsize/CD30-TNFRSF8-Antibody-1C6-Immunohistochemistry-NBP2-46157-img0013.jpg)
![Immunohistochemistry: CD30/TNFRSF8 Antibody (1C6) [NBP2-46157] - Analysis of Human colon tissue.](http://images.novusbio.com/fullsize/CD30-TNFRSF8-Antibody-1C6-Immunohistochemistry-NBP2-46157-img0014.jpg)
![Immunohistochemistry: CD30/TNFRSF8 Antibody (1C6) [NBP2-46157] - Analysis of Human lymph node tissue.](http://images.novusbio.com/fullsize/CD30-TNFRSF8-Antibody-1C6-Immunohistochemistry-NBP2-46157-img0015.jpg)
![Immunohistochemistry: CD30/TNFRSF8 Antibody (1C6) [NBP2-46157] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize/CD30-TNFRSF8-Antibody-1C6-Immunohistochemistry-NBP2-46157-img0016.jpg)
![Immunohistochemistry: CD30/TNFRSF8 Antibody (1C6) [NBP2-46157] - Analysis of Adenocarcinoma of Human colon tissue.](http://images.novusbio.com/fullsize/CD30-TNFRSF8-Antibody-1C6-Immunohistochemistry-NBP2-46157-img0017.jpg)
![Immunohistochemistry: CD30/TNFRSF8 Antibody (1C6) [NBP2-46157] - Analysis of Human thyroid tissue.](http://images.novusbio.com/fullsize/CD30-TNFRSF8-Antibody-1C6-Immunohistochemistry-NBP2-46157-img0018.jpg)
![Immunohistochemistry: CD30/TNFRSF8 Antibody (1C6) [NBP2-46157] - Analysis of Carcinoma of Human prostate tissue.](http://images.novusbio.com/fullsize/CD30-TNFRSF8-Antibody-1C6-Immunohistochemistry-NBP2-46157-img0019.jpg)
![Immunohistochemistry-Paraffin: CD59 Antibody (YTH53.1) [NB100-65537] Immunoperoxidase staining of formalin fixed, paraffin embedded human placenta with Rat anti Human CD59](http://images.novusbio.com/fullsize/CD59-Antibody-YTH53-1-Immunohistochemistry-Paraffin-NB100-65537-img0002.jpg "Immunohistochemistry-Paraffin: CD59 Antibody (YTH53.1) [NB100-65537] Immunoperoxidase staining of formalin fixed, paraffin embedded human placenta with Rat anti Human CD59")
![Flow Cytometry: CD59 Antibody (YTH53.1) [NB100-65537] - Staining of human peripheral blood granulocytes with RAT ANTI HUMAN CD59 (NB100-65537).](http://images.novusbio.com/fullsize/CD59-Antibody-YTH53-1-Flow-Cytometry-NB100-65537-img0001.jpg)
![Flow Cytometry: MCAM/CD146 Antibody (P1H12) [NBP1-43346] - A surface stain was performed on SK-MEL-28 cells with MCAM/CD146 Antibody (P1H12) NBP1-43346PCP (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to PerCP.](http://images.novusbio.com/fullsize/MCAM-CD146-Antibody-P1H12-Flow-Cytometry-NBP1-43346-img0004.jpg "Flow Cytometry: MCAM/CD146 Antibody (P1H12) [NBP1-43346] - A surface stain was performed on SK-MEL-28 cells with MCAM/CD146 Antibody (P1H12) NBP1-43346PCP (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to PerCP.")
![Flow Cytometry: MCAM/CD146 Antibody (P1H12) [NBP1-43346] - Staining of Human Umbilical Vein Endothelial Cells (HUVEC) with 0.125 ug of Mouse IgG1 K Isotype Control Purified (blue histogram) or 0.125 ug of Anti-CD146 Purified (purple histogram) followed by F(ab')2 Anti-Mouse IgG PE.Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/MCAM-CD146-Antibody-P1H12-Flow-Cytometry-NBP1-43346-img0001.jpg)
![Flow Cytometry: MCAM/CD146 Antibody (P1H12) [NBP1-43346] - Analysis using the FITC conjugate of NBP1-43346. Left: Staining of Human Umbilical Vein Endothelial Cells (HUVEC) with Mouse IgG1 K Isotype Control FITC (blue histogram) or Anti-CD146 FITC (purple histogram). Right: Staining of normal human peripheral blood cells with Anti-Human CD3 APC and Anti-CD146 FITC.](http://images.novusbio.com/fullsize/MCAM-CD146-Antibody-P1H12-Flow-Cytometry-NBP1-43346-img0003.jpg)
![Western Blot: VCAM-1/CD106 Antibody (3H10) [NBP2-03600] - Analysis of extracts (35ug) from 9 different cell lines by using anti-VCAM1 monoclonal antibody.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-3H10-Western-Blot-NBP2-03600-img0021.jpg "Western Blot: VCAM-1/CD106 Antibody (3H10) [NBP2-03600] - Analysis of extracts (35ug) from 9 different cell lines by using anti-VCAM1 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: VCAM-1/CD106 Antibody (3H10) [NBP2-03600] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY VCAM1.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-3H10-Immunocytochemistry-Immunofluorescence-NBP2-03600-img0015.jpg)
![Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (3H10) [NBP2-03600] - Staining of paraffin-embedded Human prostate tissue using anti-VCAM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-3H10-Immunohistochemistry-Paraffin-NBP2-03600-img0019.jpg)
![Western Blot: VCAM-1/CD106 Antibody (3H10) [NBP2-03600] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VCAM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VCAM1.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-3H10-Western-Blot-NBP2-03600-img0020.jpg)
![Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (3H10) [NBP2-03600] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-VCAM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-3H10-Immunohistochemistry-Paraffin-NBP2-03600-img0016.jpg)
![Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (3H10) [NBP2-03600] - CD106 / VCAM1 Antibody (3H10) [NBP2-03600] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-VCAM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-3H10-Immunohistochemistry-Paraffin-NBP2-03600-img0017.jpg)
![Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (3H10) [NBP2-03600] - Staining of paraffin-embedded Human Kidney tissue using anti-VCAM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-3H10-Immunohistochemistry-Paraffin-NBP2-03600-img0018.jpg)
![ELISA: TfR (Transferrin R) Antibody (13E4) [NB100-73092] - Titration curve of purified soluble transferrin receptor (sTfR) using pair 23D10 - 13E4. Capture: MAb 23D10 (NB110-8468); 200 ng/well Detection: MAb 13E4 conjugated with HRP Room temperature](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-13E4-ELISA-NB100-73092-img0005.jpg "ELISA: TfR (Transferrin R) Antibody (13E4) [NB100-73092] - Titration curve of purified soluble transferrin receptor (sTfR) using pair 23D10 - 13E4. Capture: MAb 23D10 (NB110-8468); 200 ng/well Detection: MAb 13E4 conjugated with HRP Room temperature")
![Western Blot: Nanog Antibody (5A10) [NBP1-04320] - The cell lysates of NIH3T3 (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Nanog (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/Nanog-Antibody-5A10-Western-Blot-NBP1-04320-img0001.jpg "Western Blot: Nanog Antibody (5A10) [NBP1-04320] - The cell lysates of NIH3T3 (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Nanog (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.")
![Flow Cytometry: Nanog Antibody (5A10) [NBP1-04320] - Flow cytometry analysis of Nanog in Hep3B cell line, staining at 2-5ug for 1x106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.](http://images.novusbio.com/fullsize/Nanog-Antibody-5A10-Flow-Cytometry-NBP1-04320-img0003.jpg)
![Immunohistochemistry-Paraffin: Nanog Antibody (5A10) [NBP1-04320] - Human seminoma tissue were incubated with anti-human Nanog (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodiumcitrate buffer and detected using Diaminobenzidine (DAB)](http://images.novusbio.com/fullsize/Nanog-Antibody-5A10-Immunohistochemistry-Paraffin-NBP1-04320-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: Nanog Antibody (5A10) - BSA Free [NBP1-04320] - Representative IF IHC-stained sections of MDBMSCC demonstrating the expression of pSTAT3 [(A), red] & EMA [(A), green] by cells within the tumor nests. There was a CSC subpopulation remonstrating nuclear co-expression of STAT3 [(B), red] & CD34 [(B), green], appearing as orange, on the endothelium of the microvessels within the stroma; & another subpopulation staining only positively for pSTAT3 within the stroma [(B), red]. Nuclear expression of NANOG [(C), red] was demonstrated on the endothelium of the microvessels which expressed CD34 [(C), green] within stroma. The NANOG+ cells [(C), red] that do not express CD34 were seen within the tumor nests & the stroma. SOX2 [(D), red] was also expressed by cells within tumor nests & the stroma, & the endothelium of the microvessels expressing CD34 [(D), green]. Nuclear expression of both SOX2 [(E), red] & SALL4 [(E), green], appearing as orange, was seen on the cells within the tumor nests & the stroma. Expression of both SOX2 [(F), red] & OCT4 [(F), green], appearing as orange, was seen on cells within the tumor nests & the stroma, & the endothelium of the microvessels within the stroma. pSTAT3 [(G), red] & membranous staining CD44 [(G), green] were co-expressed by cells within the tumor nests. Scale bars: 20 μm. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fsurg.2016.00046/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-04320_mouse-nanog-mab-5a10-bsa-free-310202416165642.jpg)
![Western Blot: 14-3-3 gamma Antibody (AT4B9) [NBP2-50579] - Lane 1.: 293T cell lysate Lane 2.: A431 cell lysate Lane 3.: K562 cell lysate Lane 4.: NIH3T3 cell lysate](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-AT4B9-Western-Blot-NBP2-50579-img0003.jpg "Western Blot: 14-3-3 gamma Antibody (AT4B9) [NBP2-50579] - Lane 1.: 293T cell lysate Lane 2.: A431 cell lysate Lane 3.: K562 cell lysate Lane 4.: NIH3T3 cell lysate")
![Immunocytochemistry/Immunofluorescence: 14-3-3 gamma Antibody (AT4B9) [NBP2-50579] - Analysis of 14-3-3 gamma in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human 14-3-3 gamma antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-AT4B9-Immunocytochemistry-Immunofluorescence-NBP2-50579-img0004.jpg)
![Western Blot: 14-3-3 gamma Antibody (AT4B9) [NBP2-50579] - The lysates of Hela (40ug) and Mouse brain (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human 14-3-3 gamma antibody (1:1000) Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-AT4B9-Western-Blot-NBP2-50579-img0001.jpg)
![Western Blot: 14-3-3 gamma Antibody (AT4B9) [NBP2-50579] - The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PDVF membrane and probed with anti-human 14-3-3 gamma antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. Recombinant Human YWHAZ Lane 2. Recombinant Human YWHAB Lane 3. Recombinant Human YWHAE Lane 4. Recombinant Human YWHAH Lane 5.: Recombinant Human YWHAG Lane 6.: Recombinant Human SFN Lane 7.: Recombinant Human YWHAQ.](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-AT4B9-Western-Blot-NBP2-50579-img0002.jpg)