![Immunocytochemistry/Immunofluorescence: C20orf27 Antibody [NBP2-14399] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/C20orf27-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-14399-img0004.jpg "Immunocytochemistry/Immunofluorescence: C20orf27 Antibody [NBP2-14399] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & cytosol.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: LHVKLLSVVPVPEGYSVKCEYSAHKEGVLKA |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | C20ORF27 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100. |
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| Control Peptide |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for C20orf27 Antibody (NBP2-14399)Find related products by research area.
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| Gene Symbol | C20ORF27 |
![Immunohistochemistry-Paraffin: C20orf27 Antibody [NBP2-14399] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp2-14399_rabbit-polyclonal-c20orf27-antibody-immunohistochemistry-paraffin-21920237337..jpg "Immunohistochemistry-Paraffin: C20orf27 Antibody [NBP2-14399] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.")
![Immunohistochemistry-Paraffin: C20orf27 Antibody [NBP2-14399] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp2-14399_rabbit-polyclonal-c20orf27-antibody-immunohistochemistry-paraffin-2192023750..jpg "Immunohistochemistry-Paraffin: C20orf27 Antibody [NBP2-14399] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: C20orf27 Antibody [NBP2-14399] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/antibody/nbp2-14399_rabbit-polyclonal-c20orf27-antibody-immunohistochemistry-paraffin-21920237642..jpg "Immunohistochemistry-Paraffin: C20orf27 Antibody [NBP2-14399] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.")
![Immunohistochemistry-Paraffin: C20orf27 Antibody [NBP2-14399] - Staining of human placenta shows no positivity in trophoblastic cells as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-14399_rabbit-polyclonal-c20orf27-antibody-immunohistochemistry-paraffin-21920237748..jpg "Immunohistochemistry-Paraffin: C20orf27 Antibody [NBP2-14399] - Staining of human placenta shows no positivity in trophoblastic cells as expected.")
![Western Blot: C20orf27 Antibody [NBP2-14399] -Analysis in human cell line NB4.](http://images.novusbio.com/fullsize/antibody/nbp2-14399_rabbit-polyclonal-c20orf27-antibody-western-blot-21920237854..jpg "Western Blot: C20orf27 Antibody [NBP2-14399] -Analysis in human cell line NB4.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")