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![Immunohistochemistry-Paraffin: VPS29 Antibody [NB100-41109] - Staining of paraffin embedded Human Placenta. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/VPS29-Antibody-Immunohistochemistry-Paraffin-NB100-41109-img0003.jpg "Immunohistochemistry-Paraffin: VPS29 Antibody [NB100-41109] - Staining of paraffin embedded Human Placenta. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Immunohistochemistry-Paraffin: VPS29 Antibody [NB100-41109] - Staining of paraffin embedded Human Uterus. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/VPS29-Antibody-Immunohistochemistry-Paraffin-NB100-41109-img0002.jpg)
![Western Blot: PUMA Antibody [NBP1-52093] - (0.1ug/ml) staining of Peripheral Blood Lymphocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PUMA-Antibody-Western-Blot-NBP1-52093-img0002.jpg "Western Blot: PUMA Antibody [NBP1-52093] - (0.1ug/ml) staining of Peripheral Blood Lymphocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: DUSP14 Antibody [NB100-887] - Staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/DUSP14-Antibody-Immunohistochemistry-Paraffin-NB100-887-img0001.jpg "Immunohistochemistry-Paraffin: DUSP14 Antibody [NB100-887] - Staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Western Blot: Alkaline Phosphatase, Intestinal Antibody [NB120-7325] - Western Blot of Rabbit anti-Alkaline Phosphatase antibody Biotin Conjugated. Lane 1: Alkaline Phosphatase (calf intestine). Load: 50 ng per lane.Primary antibody: Alkaline Phosphatase antibody Biotin conjugated at 1:2,000 for 60 min at RT.Secondary antibody: Peroxidase Streptavidin secondary antibody at 1:40,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 57 kDa, 57 kDa for Alkaline Phosphatase.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Intestinal-Antibody-Western-Blot-NB120-7325-img0007.jpg "Western Blot: Alkaline Phosphatase, Intestinal Antibody [NB120-7325] - Western Blot of Rabbit anti-Alkaline Phosphatase antibody Biotin Conjugated. Lane 1: Alkaline Phosphatase (calf intestine). Load: 50 ng per lane.Primary antibody: Alkaline Phosphatase antibody Biotin conjugated at 1:2,000 for 60 min at RT.Secondary antibody: Peroxidase Streptavidin secondary antibody at 1:40,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 57 kDa, 57 kDa for Alkaline Phosphatase.")
![Western Blot: Alkaline Phosphatase, Intestinal Antibody [NB120-7325] - Lane 1: Alkaline Phosphatase (Calf Intestine). Load: 50 ng per lane. Primary antibody: Rabbit anti-Alkaline Phosphatase (Calf Intestine) Antibody at 1:1,000 overnight at 4C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 57 kDa, 60 kDa for Alkaline Phosphatase (Calf Intestine).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Intestinal-Antibody-Western-Blot-NB120-7325-img0001.jpg)
![Western Blot: Alkaline Phosphatase, Intestinal Antibody [NB120-7325] - Lane 1: Calf intestine Alkaline Phosphatase. Load: 1.5 ug Block: 5% Blotto 1 hour at 4C. Primary antibody: diluted to 1:1000 in 5% Blotto overnight at 4C. Secondary antibody: Goat anti Rabbit 1:20,000 in 1 Hour 4C Substrate: Femtomax 110.](http://images.novusbio.com/fullsize/Alkaline Phosphatase- Intestinal Antibody-Western Blot-NB120-7325-img0008.jpg)
![Western Blot: CTDSP2 Antibody [NBP1-32283] - A. 30 ug PC-12 whole cell lysate/extract. 12 % SDS-PAGE](http://images.novusbio.com/fullsize/CTDSP2-Antibody-Western-Blot-NBP1-32283-img0005.jpg "Western Blot: CTDSP2 Antibody [NBP1-32283] - A. 30 ug PC-12 whole cell lysate/extract. 12 % SDS-PAGE")
![Western Blot: CTDSP2 Antibody [NBP1-32283] - Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/CTDSP2-Antibody-Western-Blot-NBP1-32283-img0002.jpg)
![Western Blot: CTDSP2 Antibody [NBP1-32283] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE CTDSP2 antibody, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/CTDSP2-Antibody-Western-Blot-NBP1-32283-img0003.jpg)
![Western Blot: PPP2R1A Antibody [NB100-41408] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiPPP2R1A antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PPP2R1A-Antibody-Western-Blot-NB100-41408-img0004.jpg "Western Blot: PPP2R1A Antibody [NB100-41408] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiPPP2R1A antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: PPP2R1A Antibody [NB100-41408] - Detection of human PPP2R1A by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PPP2R1A antibody NB100-41408 used for IP at 3 ug per reaction. PPP2R1A was also immunoprecipitated by rabbit anti-PPP2R1A antibody NB100-41407. For blotting immunoprecipitated PPP2R1A, NB100-41408 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PPP2R1A-Antibody-Immunoprecipitation-NB100-41408-img0005.jpg)
![Western Blot: FCP1 Antibody [NBP1-31472] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/FCP1-Antibody-Western-Blot-NBP1-31472-img0006.jpg "Western Blot: FCP1 Antibody [NBP1-31472] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 7. 5% SDS PAGE; antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: FCP1 Antibody [NBP1-31472] - Paraffin-embedded HeLa xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/FCP1-Antibody-Immunohistochemistry-Paraffin-NBP1-31472-img0005.jpg)
![Immunohistochemistry-Paraffin: FCP1 Antibody [NBP1-31472] - Paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/FCP1-Antibody-Immunohistochemistry-Paraffin-NBP1-31472-img0004.jpg)
![Immunoprecipitation: MEK1 Antibody (9B6) [NBP3-26153] - Immunoprecipitating MEK1 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26153 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26153 (2ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).](http://images.novusbio.com/fullsize/nbp3-26153_rabbit-mek1-mab-9b6-2622024132378.jpg "Immunoprecipitation: MEK1 Antibody (9B6) [NBP3-26153] - Immunoprecipitating MEK1 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26153 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26153 (2ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).")
![Immunohistochemistry: MEK1 Antibody (9B6) [NBP3-26153] - Image of MEK1 Antibody (9B6) diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26153_rabbit-mek1-mab-9b6-2722024857336.jpg)
![Western Blot: MEK1 Antibody (9B6) [NBP3-26153] - Positive Western Blot detected in: Hela whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, 293T whole cell lysate, A549 whole cell lysate, U251 whole cell lysate, Rat brain tissue. All lanes: MEK1 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 44, 41 kDa Observed band size: 44 kDa](http://images.novusbio.com/fullsize/nbp3-26153_rabbit-mek1-mab-9b6-28220241147210.jpg)
![Western Blot: HISPPD1 Antibody [NBP2-16836] - Sample (30 ug of whole cell lysate) A: Raji B: K562 5% SDS PAGE gel, diluted at 1:3000.](http://images.novusbio.com/fullsize/HISPPD1-Antibody-Western-Blot-NBP2-16836-img0001.jpg "Western Blot: HISPPD1 Antibody [NBP2-16836] - Sample (30 ug of whole cell lysate) A: Raji B: K562 5% SDS PAGE gel, diluted at 1:3000.")
![Immunohistochemistry-Paraffin: HISPPD1 Antibody [NBP2-16836] - Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/HISPPD1-Antibody-Immunohistochemistry-Paraffin-NBP2-16836-img0002.jpg)
![Western Blot: ATAD3A Antibody [NBP2-14881] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE; antibody diluted at 1:5000.](http://images.novusbio.com/fullsize/ATAD3A-Antibody-Western-Blot-NBP2-14881-img0002.jpg "Western Blot: ATAD3A Antibody [NBP2-14881] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE; antibody diluted at 1:5000.")
![Immunohistochemistry-Paraffin: ATAD3A Antibody [NBP2-14881] - Paraffin-embedded colon carcinoma. ATAD3A antibody dilution: 1:500.](http://images.novusbio.com/fullsize/ATAD3A-Antibody-Immunohistochemistry-Paraffin-NBP2-14881-img0003.jpg)
![Western Blot: PHOSPHO1 Antibody (4B2) [H00162466-M04] - Analysis of PHOSPHO1 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/PHOSPHO1-Antibody-4B2-Western-Blot-H00162466-M04-img0004.jpg "Western Blot: PHOSPHO1 Antibody (4B2) [H00162466-M04] - Analysis of PHOSPHO1 expression in PC-12 (Cat # L012V1).")
![Western Blot: PHOSPHO1 Antibody (4B2) [H00162466-M04] - Analysis of PHOSPHO1 expression in Jurkat (Cat # L017V1).](http://images.novusbio.com/fullsize/PHOSPHO1-Antibody-4B2-Western-Blot-H00162466-M04-img0005.jpg)
![ELISA: PHOSPHO1 Antibody (4B2) [H00162466-M04] - Detection limit for recombinant GST tagged PHOSPHO1 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PHOSPHO1-Antibody-4B2-ELISA-H00162466-M04-img0003.jpg)
![Western Blot: Eosinophil derived neurotoxin Antibody (HL2166) - Azide and BSA Free [NBP3-25453] - Untreated (-) and treated (+) THP-1 whole cell extract (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Eosinophil derived neurotoxin antibody [HL2166] (NBP3-25453) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25453_rabbit-eosinophil-derived-neurotoxin-mab-hl2166-azide-bsa-free-2120249435511.jpg "Western Blot: Eosinophil derived neurotoxin Antibody (HL2166) - Azide and BSA Free [NBP3-25453] - Untreated (-) and treated (+) THP-1 whole cell extract (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Eosinophil derived neurotoxin antibody [HL2166] (NBP3-25453) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Eosinophil derived neurotoxin Antibody (HL2166) - Azide and BSA Free [NBP3-25453] - Untreated (-) and treated (+) THP-1 whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Eosinophil derived neurotoxin antibody [HL2166] (NBP3-25453) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25453_rabbit-eosinophil-derived-neurotoxin-mab-hl2166-azide-bsa-free-2120249374932.jpg)
![Immunohistochemistry-Paraffin: Eosinophil derived neurotoxin Antibody (HL2166) - Azide and BSA Free [NBP3-25453] - Eosinophil derived neurotoxin antibody [HL2166] detects Eosinophil derived neurotoxin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human kidney. Eosinophil derived neurotoxin stained by Eosinophil derived neurotoxin antibody [HL2166] (NBP3-25453) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25453_rabbit-eosinophil-derived-neurotoxin-mab-hl2166-azide-bsa-free-2120249422640.jpg)