| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB110-61018-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2079SS | |||
| Azide and BSA Free | NBP2-80732 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-22131SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-81943-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82685-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90162-25ul | |||
![Immunohistochemistry-Paraffin: VEGF Receptor 3 Antibody (RM0003-5F63) [NB110-61018] - 4% PFA fixed mouse heart and kidney tissue sections were subjected to IHC with rat anti mouse VEGFR3 (RM0003-5F63).](http://images.novusbio.com/fullsize/VEGF-Receptor-3-Antibody-RM0003-5F63-Immunohistochemistry-Paraffin-NB110-61018-img0001.jpg "Immunohistochemistry-Paraffin: VEGF Receptor 3 Antibody (RM0003-5F63) [NB110-61018] - 4% PFA fixed mouse heart and kidney tissue sections were subjected to IHC with rat anti mouse VEGFR3 (RM0003-5F63).")
![Western Blot: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Analysis of PI 3 Kinase p85 alpha on different lysates using anti-PI 3 Kinase p85 alpha antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: Raji](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-SU04-07-Western-Blot-NBP2-67488-img0010.jpg "Western Blot: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Analysis of PI 3 Kinase p85 alpha on different lysates using anti-PI 3 Kinase p85 alpha antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: Raji")
![Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Staining PI 3 Kinase p85 alpha in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-SU04-07-Immunocytochemistry-Immunofluorescence-NBP2-67488-img0005.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Analysis of paraffin-embedded mouse kidney tissue using anti-PI 3 Kinase p85 alpha antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-SU04-07-Immunohistochemistry-Paraffin-NBP2-67488-img0009.jpg)
![Flow Cytometry: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Analysis of HepG2 cells with PI 3 Kinase p85 alpha antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antib](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-SU04-07-Flow-Cytometry-NBP2-67488-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Staining PI 3 Kinase p85 alpha in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-SU04-07-Immunocytochemistry-Immunofluorescence-NBP2-67488-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Staining PI 3 Kinase p85 alpha in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-SU04-07-Immunocytochemistry-Immunofluorescence-NBP2-67488-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Staining PI 3 Kinase p85 alpha in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-SU04-07-Immunocytochemistry-Immunofluorescence-NBP2-67488-img0004.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Analysis of paraffin-embedded human kidney tissue using anti-PI 3 Kinase p85 alpha antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-SU04-07-Immunohistochemistry-Paraffin-NBP2-67488-img0006.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Analysis of paraffin-embedded human liver cancer tissue using anti-PI 3 Kinase p85 alpha antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-SU04-07-Immunohistochemistry-Paraffin-NBP2-67488-img0007.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Analysis of paraffin-embedded mouse heart tissue using anti-PI 3 Kinase p85 alpha antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-SU04-07-Immunohistochemistry-Paraffin-NBP2-67488-img0008.jpg)
![Western Blot: CrkRS Antibody [NB100-87011] - Detection of Human and Mouse CrkRS by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CrkRS antibody NB100-87011 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CrkRS-Antibody-Western-Blot-NB100-87011-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: CrkRS Antibody [NB100-87011] - Detection of Human and Mouse CrkRS by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CrkRS antibody NB100-87011 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: CrkRS Antibody [NB100-87011] - Detection of human CrkRS by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CrkRS antibody NB100-87011 used for IP at 6 ug per reaction. For blotting immunoprecipitated CrkRS, NB100-87011 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CrkRS-Antibody-NB100-87011-img0005.jpg)
![Western Blot: FGF R1 Antibody (M17A3) [NB100-2079] - Whole cell protein from HepG2 (lane 1), 3T3 (lane 2) and PC12 (lane 3) was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 4.0 ug/ml anti-FGFR1 in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/FGF-R1-Antibody-M17A3-Western-Blot-NB100-2079-img0004.jpg "Western Blot: FGF R1 Antibody (M17A3) [NB100-2079] - Whole cell protein from HepG2 (lane 1), 3T3 (lane 2) and PC12 (lane 3) was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 4.0 ug/ml anti-FGFR1 in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![Immunohistochemistry-Paraffin: FGF R1 Antibody (M17A3) [NB100-2079] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human normal skin using mouse monoclonal FGF R1 Antibody (clone M17A3) at 5 ug/ml concentration. The keratinocytes of the epidermal layer showed a very strong membrane-cytoplasmic with relatively weak nuclear staining for FGF R1 Antibody. The stratum basale layer of keratinocytes depicted more of membrane-cytoplasmic staining for this protein.](http://images.novusbio.com/fullsize/FGF-R1-Antibody-M17A3-Immunohistochemistry-Paraffin-NB100-2079-img0003.jpg)
![Flow Cytometry: FGF R1 Antibody (M17A3) [NB100-2079] - Analysis of FGF R1 in HEK293 cells (2x10^6 cells/ml) were stained with FGF R1 antibody (NB100-2079, red) at 1:1000 dilution. Detected with FITC conjugated goat anti-mouse IgG1 isotype control (green). Two distinct samples shown.](http://images.novusbio.com/fullsize/FGF-R1-Antibody-M17A3-Flow-Cytometry-NB100-2079-img0006.jpg)
![Flow Cytometry: FGF R1 Antibody (M17A3) [NB100-2079] - Analysis of FGF R1 in MCF-7 cells (1x10^6 cells/ml) were stained with FGF R1 antibody (NB100-2079, red) at 1:1000 dilution. Detected with FITC conjugated goat anti-mouse IgG1 isotype control (green). Two distinct samples shown.](http://images.novusbio.com/fullsize/FGF-R1-Antibody-M17A3-Flow-Cytometry-NB100-2079-img0005.jpg)
![Western Blot: CaMKII beta Antibody [NB120-22131] - Analysis of CAMKIIb in rat brain lysate using NB120-22131 at 1:2000.](http://images.novusbio.com/fullsize/CaMKII-beta-Antibody-Western-Blot-NB120-22131-img0002.jpg "Western Blot: CaMKII beta Antibody [NB120-22131] - Analysis of CAMKIIb in rat brain lysate using NB120-22131 at 1:2000.")
![Immunocytochemistry/Immunofluorescence: CaMKII beta Antibody [NB120-22131] - Staining of CAMKIIb using NB120-22131 in rat hippocampal neurons at a dilution of 1:100, note that staining is rather weak.](http://images.novusbio.com/fullsize/CaMKII-beta-Antibody-Immunocytochemistry-Immunofluorescence-NB120-22131-img0001.jpg)
![Western Blot: CaMKII beta Antibody [NB120-22131] - Analysis of CAMKII beta in A) human, B) mouse and C) rat brain lysate using CAMKII beta antibody at 1:2000.](http://images.novusbio.com/fullsize/CaMKII-beta-Antibody-Western-Blot-NB120-22131-img0003.jpg)
![Immunohistochemistry-Paraffin: Sin1/MAPKAP1 Antibody [NB110-40424] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- Sin1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Sin1-MAPKAP1-Antibody-Immunohistochemistry-Paraffin-NB110-40424-img0009.jpg)
![Western Blot: Sin1/MAPKAP1 Antibody [NB110-40424] - Detection of Human Sin1 on HeLa whole cell lysate.](http://images.novusbio.com/fullsize/Sin1-MAPKAP1-Antibody-Western-Blot-NB110-40424-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: GSK-3 alpha Antibody [NB100-81943] - Staining of methanol-fixed MCF7 cells NB100-81943](http://images.novusbio.com/fullsize/GSK-3-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NB100-81943-img0006.jpg "Immunocytochemistry/Immunofluorescence: GSK-3 alpha Antibody [NB100-81943] - Staining of methanol-fixed MCF7 cells NB100-81943")
![Immunoprecipitation: GSK-3 alpha Antibody [NB100-81943] - Human HaCaT keratinocyte lysates with GSK3 alpha Antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/GSK-3-alpha-Antibody-Immunoprecipitation-NB100-81943-img0010.jpg)
![Western Blot: GSK-3 alpha Antibody [NB100-81943] - Analysis of extracts from 293 cells using NB100-81943](http://images.novusbio.com/fullsize/GSK-3-alpha-Antibody-Western-Blot-NB100-81943-img0008.jpg)
![Immunohistochemistry-Paraffin: GSK-3 alpha Antibody [NB100-81943] - Analysis of paraffin- embedded human breast carcinoma tissue using NB100-81943(left) or the same antibody preincubated with blocking peptide (right).](http://images.novusbio.com/fullsize/nb100-81943_rabbit-polyclonal-gsk-3-alpha-antibody-10320252332100.jpg)
![Western Blot: Fyn Antibody [NBP1-82685] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Fyn-Antibody-Western-Blot-NBP1-82685-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Fyn Antibody [NBP1-82685] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Fyn Antibody [NBP1-82685] - Staining of human cell line AF22 shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Fyn-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82685-img0009.jpg)
![Immunohistochemistry-Paraffin: Fyn Antibody [NBP1-82685] - Staining of human tonsil shows strong cytoplasmic positivity in a subset of reaction center cells and lymphoid cells outside reaction centra.](http://images.novusbio.com/fullsize/Fyn-Antibody-Immunohistochemistry-Paraffin-NBP1-82685-img0002.jpg)
![Western Blot: Fyn Antibody [NBP1-82685] - Analysis in human cell line U-87 MG.](http://images.novusbio.com/fullsize/Fyn-Antibody-Western-Blot-NBP1-82685-img0007.jpg)
![Western Blot: Fyn Antibody [NBP1-82685] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Fyn-Antibody-Western-Blot-NBP1-82685-img0008.jpg)
![Immunohistochemistry-Paraffin: Fyn Antibody [NBP1-82685] -Staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.](http://images.novusbio.com/fullsize/antibody/nbp1-82685_rabbit-polyclonal-fyn-antibody-immunohistochemistry-paraffin-422025132224..png)
![Immunohistochemistry-Paraffin: Fyn Antibody [NBP1-82685] - Staining of human kidney shows moderate cytoplasmic positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/antibody/nbp1-82685_rabbit-polyclonal-fyn-antibody-immunohistochemistry-paraffin-422025132315..png)
![Immunohistochemistry-Paraffin: Fyn Antibody [NBP1-82685] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/antibody/nbp1-82685_rabbit-polyclonal-fyn-antibody-immunohistochemistry-paraffin-42202513248..png)
![Simple Western: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Detection of human CD247/CD3Z by Simple Western. Samples: Whole cell lysate (2.0 mg/mL) from Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] (NBP2-76399) used at 1:10, 1:50, 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.](http://images.novusbio.com/fullsize/nbp2-76399_rabbit-monoclonal-cd3-zeta-antibody-bl-336-1b2-simple-western-3032023112047.jpg "Simple Western: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Detection of human CD247/CD3Z by Simple Western. Samples: Whole cell lysate (2.0 mg/mL) from Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] (NBP2-76399) used at 1:10, 1:50, 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.")
![Flow Cytometry: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Jurkat cells were fixed in 4% formaldehyde and permeabilized with 90% methanol. Antibody: 1E6 cells were incubated with 0.5 ul of rabbit anti-CD247/CD3Zrecombinant monoclonal antibody [BL-336-1B2] (red shaded) or isotype control(unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-BL-336-1B2-Flow-Cytometry-NBP2-76399-img0007.jpg)
![Immunohistochemistry: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Section of human tonsil. Antibody: Rabbit anti-CD247/CD3Z recombinantmonoclonal antibody [BL-336-1B2] used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-BL-336-1B2-Immunohistochemistry-NBP2-76399-img0006.jpg)
![Immunohistochemistry-Paraffin: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Antibody: Rabbit anti-CD247/CD3Z recombinant monoclonal [BL-336-1B2].Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-BL-336-1B2-Immunohistochemistry-Paraffin-NBP2-76399-img0009.jpg)
![Immunoprecipitation: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Detection of human CD247/CD3Z by WB of immunoprecipitates from Jurkat lysate.Antibodies: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] (NBP2-76399).](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-BL-336-1B2-Immunoprecipitation-NBP2-76399-img0008.jpg)
![Immunohistochemistry-Paraffin: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Rabbit antiCD247/CD3Z recombinant monoclonal antibody[BL-336-1B2]. (Secondary: HRPconjugated goat anti-rabbit IgG. Substrate:DAB.](http://images.novusbio.com/fullsize/nbp2-76399_rabbit-monoclonal-cd3-zeta-antibody-bl-336-1b2-immunohistochemistry-paraffin-732024101321..jpg)
![Western Blot: CD3 zeta Antibody (BL-336-1B2) [NBP2-76399] - Whole cell lysate (10 ug) from Jurkat, U2OS, SR, HEK293T, and MOLT-4 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit antiCOPB2 antibody.](http://images.novusbio.com/fullsize/nbp2-76399_rabbit-monoclonal-cd3-zeta-antibody-bl-336-1b2-western-blot-732024101522..jpg)
![Immunohistochemistry-Paraffin: Annexin A1 Antibody [NBP1-90162] - Staining of human colon, esophagus, lung and lymph node using Anti-Annexin A1 antibody NBP1-90162 (A) shows similar protein distribution across tissues to independent antibody NBP1-90161 (B).](http://images.novusbio.com/fullsize/Annexin-A1-Antibody-Immunohistochemistry-Paraffin-NBP1-90162-img0028.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Annexin A1 Antibody [NBP1-90162] - Staining of human colon, esophagus, lung and lymph node using Anti-Annexin A1 antibody NBP1-90162 (A) shows similar protein distribution across tissues to independent antibody NBP1-90161 (B).")
![Immunohistochemistry-Paraffin: Annexin A1 Antibody [NBP1-90162] - Staining of human esophagus shows moderate to strong positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Annexin-A1-Antibody-Immunohistochemistry-Paraffin-NBP1-90162-img0022.jpg)
![Immunohistochemistry-Paraffin: Annexin A1 Antibody [NBP1-90162] - Staining of human colon shows moderate positivity in lymphoid cells.](http://images.novusbio.com/fullsize/Annexin-A1-Antibody-Immunohistochemistry-Paraffin-NBP1-90162-img0024.jpg)
![Immunohistochemistry-Paraffin: Annexin A1 Antibody [NBP1-90162] - Staining of human lung shows strong positivity in macrophages.](http://images.novusbio.com/fullsize/Annexin-A1-Antibody-Immunohistochemistry-Paraffin-NBP1-90162-img0020.jpg)
![Western Blot: CD3 zeta Antibody (H146-968) - Azide Free [NBP1-44683] - Analysis of CD3 zeta (CD247) in lysate of murine splenocytes (reducing conditions) by the monoclonal antibody H146-968.](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-H146-968-Azide-Free-Western-Blot-NBP1-44683-img0001.jpg "Western Blot: CD3 zeta Antibody (H146-968) - Azide Free [NBP1-44683] - Analysis of CD3 zeta (CD247) in lysate of murine splenocytes (reducing conditions) by the monoclonal antibody H146-968.")
![Flow Cytometry: CD3 zeta Antibody (H146-968) - Azide Free [NBP1-44683] - Analysis (intracellular staining) of CD3 zeta (CD247) in human peripheral blood using the monoclonal antibody H146-968 PE](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-H146-968-Azide-Free-Flow-Cytometry-NBP1-44683-img0003.jpg)
![Flow Cytometry: CD3 zeta Antibody (H146-968) - Azide Free [NBP1-44683] - Flow Cytometry: CD3 zeta Antibody (H146-968) [PE] [NBP1-44683PE] - A cell surface stain was performed on hPBMCs with CD3 Zeta antibody (H146-968) NBP1-44683 (top image) and a matched isotype control (bottom image). Cells were incubated in an antibody dilution of 1:200 for 20 minutes at room temperature. Both antibodies were conjugated to PE.](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-H146-968-Azide-Free-Flow-Cytometry-NBP1-44683-img0002.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of rat fore brain. Histone H3K18ac (acetyl Lys18) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Immunohistochemistry-Paraffin-NBP2-43535-img0007.jpg "Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of rat fore brain. Histone H3K18ac (acetyl Lys18) antibody dilution: 1:500.")
![<b>Biological Strategies Validation. </b>Chromatin Immunoprecipitation: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Cross-linked ChIP was performed with HeLa chromatin extract treated with Trichostatin A (0.4 uM for 18 h) and 5 ug of either control rabbit IgG or anti-Histone H3K18ac (acetyl Lys18) antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or beta-globin promoter.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Chromatin-Immunoprecipitation-NBP2-43535-img0001.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of mouse duodenum. Histone H3K18ac (acetyl Lys18) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Immunohistochemistry-Paraffin-NBP2-43535-img0004.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of mouse prostate. Histone H3K18ac (acetyl Lys18) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Immunohistochemistry-Paraffin-NBP2-43535-img0006.jpg)
![<b>Biological Strategies Validation. </b>Immunoprecipitation: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of HeLa whole cell lysate/extract: A. 30 ug whole cell lysate/extract of Histone H3 (acetyl Lys18) protein expressing HeLa cells (0.4uM TSA treatment for 18hr) B. Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of Histone H3 (acetyl Lys18) protein by 2.5 ug of Histone H3 (acetyl Lys18) antibody 12% SDS-PAGE The immunoprecipitated Histone H3 protein (acetyl Lys18) was detected by Histone H3 (acetyl Lys18) antibody. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Immunoprecipitation-NBP2-43535-img0008.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects HIST1H3A protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.HIST1H3A stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-910202420122076.jpg)
![Immunocytochemistry/ Immunofluorescence: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects HIST1H3A protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HIST1H3A stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-91020242014689.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects Histone H3K18ac protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse stomach.Histone H3K18ac stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-910202420115031.jpg)
![Immunocytochemistry/ Immunofluorescence: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects HIST1H3A protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HIST1H3A stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-91020242014664.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects HIST1H3A protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.HIST1H3A stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-910202420115087.jpg)
![Immunocytochemistry/ Immunofluorescence: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects HIST1H3A protein at chromosome by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HIST1H3A stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-91020242011459.jpg)
![Immunocytochemistry/ Immunofluorescence: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects HIST1H3A protein at chromosome by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HIST1H3A stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-9102024205654.jpg)
![Histone H3 (acetyl Lys18) antibody detects Histone H3 (acetyl Lys18) protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Histone H3 (acetyl Lys18) protein stained by Histone H3 (acetyl Lys18) antibody (NBP2-43535) diluted at 1:500.Red: alpha Tubulin antibody [GT114], a cytoskeleton marker, stained by diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-immunocytochemistry-immunofluorescence-261120251823621.jpg)
