| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38021-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38164-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12036-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82439-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87375-25ul | |||
![Immunohistochemistry: EAAT3 Antibody [NBP3-38021] - Perform microwave antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded mouse brain using SLC1A1 Rabbit pAb at dilution of 1:400 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38021_rabbit-eaat3-pab-10122024140439.jpg "Immunohistochemistry: EAAT3 Antibody [NBP3-38021] - Perform microwave antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded mouse brain using SLC1A1 Rabbit pAb at dilution of 1:400 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: EAAT3 Antibody [NBP3-38021] - Western blot analysis of various lysates using at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-38021_rabbit-eaat3-pab-10122024140454.jpg)
![Western Blot: FZR1/CDH1 Antibody [NBP3-38164] - Western blot analysis of lysates from 293T cells, using FZR1/CDH1 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-38164_rabbit-fzr1-cdh1-pab-1012202411421066.jpg "Western Blot: FZR1/CDH1 Antibody [NBP3-38164] - Western blot analysis of lysates from 293T cells, using FZR1/CDH1 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s.")
![Immunocytochemistry/ Immunofluorescence: FZR1/CDH1 Antibody [NBP3-38164] - Immunofluorescence analysis of HeLa cells using FZR1/CDH1 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38164_rabbit-fzr1-cdh1-pab-1012202411353070.jpg)
![Western Blot: E-Cadherin [p Ser838, p Ser840] Antibody (S03-3B3) [NBP3-19826] - Western blot detection of E-Cadherin (phospho-Ser838/Ser840) in HepG2, U2OS, U251, Lncap using NBP3-19826 (1:1000 diluted)](http://images.novusbio.com/fullsize/E-Cadherin-p-Ser838-p-Ser840-Antibody-S03-3B3-Western-Blot-NBP3-19826-img0002.jpg "Western Blot: E-Cadherin [p Ser838, p Ser840] Antibody (S03-3B3) [NBP3-19826] - Western blot detection of E-Cadherin (phospho-Ser838/Ser840) in HepG2, U2OS, U251, Lncap using NBP3-19826 (1:1000 diluted)")
![Immunohistochemistry-Paraffin: E-Cadherin [p Ser838, p Ser840] Antibody (S03-3B3) [NBP3-19826] - Immunohistochemistry of E-Cadherin (phospho-Ser838/Ser840) in paraffin-embedded Human breast cancer tissue using NBP3-19826 at dilution 1/50](http://images.novusbio.com/fullsize/E-Cadherin-p-Ser838-p-Ser840-Antibody-S03-3B3-Immunohistochemistry-Paraffin-NBP3-19826-img0001.jpg)
![Western Blot: Sodium Potassium ATPase Beta 1 Antibody (S02-8B6) [NBP3-20085] - Western blot detection of Sodium Potassium ATPase Beta 1 in Rat Brain lysates using NBP3-20085 (1:1000 diluted). Predicted band size: 35kDa. Observed band size: 45-55kDa.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Beta-1-Antibody-S02-8B6-Western-Blot-NBP3-20085-img0002.jpg "Western Blot: Sodium Potassium ATPase Beta 1 Antibody (S02-8B6) [NBP3-20085] - Western blot detection of Sodium Potassium ATPase Beta 1 in Rat Brain lysates using NBP3-20085 (1:1000 diluted). Predicted band size: 35kDa. Observed band size: 45-55kDa.")
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody (S02-8B6) [NBP3-20085] - Immunohistochemistry of Sodium Potassium ATPase Beta 1 in paraffin-embedded Human colon cancer tissue using NBP3-20085 at dilution 1/20](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Beta-1-Antibody-S02-8B6-Immunohistochemistry-Paraffin-NBP3-20085-img0001.jpg)
![Western Blot: CD98 Antibody (RL388) - Chimeric [NBP3-12036] - Mouse fetal liver lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric rabbit IgG version of RL388 (NBP3-12036) at 0.05 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/CD98-Antibody-RL388-Chimeric-Western-Blot-NBP3-12036-img0001.jpg "Western Blot: CD98 Antibody (RL388) - Chimeric [NBP3-12036] - Mouse fetal liver lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric rabbit IgG version of RL388 (NBP3-12036) at 0.05 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry /Immunofluorescence: CD98 Antibody (RL388) - Chimeric - Azide and BSA Free [NBP3-12036] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with NBP3-12036 at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12036, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (NBP2-52638) followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-12036_rabbit-monoclonal-cd98-antibody-rl388-chimeric-74202522141236.png)
![SDS-Page: Recombinant Human P2Y1/P2RY1 Protein [H00005028-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00005028-Q01-1.jpg "SDS-Page: Recombinant Human P2Y1/P2RY1 Protein [H00005028-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: VE-Cadherin Antibody (PSH03-15) [NBP3-33088] - Western blot analysis of VE-Cadherin on different lysates with Rabbit anti-VE-Cadherin antibody (NBP3-33088) at 1/2,000 dilution. Lane 1: EA.hy926-si NT cell lysate Lane 2: EA.hy926-si VE Cadherin cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 88 kDa Observed band size: 140 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33088) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33088_rabbit-ve-cadherin-mab-psh03-15-288202414191513.jpg "Western Blot: VE-Cadherin Antibody (PSH03-15) [NBP3-33088] - Western blot analysis of VE-Cadherin on different lysates with Rabbit anti-VE-Cadherin antibody (NBP3-33088) at 1/2,000 dilution. Lane 1: EA.hy926-si NT cell lysate Lane 2: EA.hy926-si VE Cadherin cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 88 kDa Observed band size: 140 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33088) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: VE-Cadherin Antibody (PSH03-15) [NBP3-33088] - Immunocytochemistry analysis of HeLa cells labeling VE-Cadherin with Rabbit anti-VE-Cadherin antibody (NBP3-33088) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-VE-Cadherin antibody (NBP3-33088) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33088_rabbit-ve-cadherin-mab-psh03-15-29202410234272.jpg)
![Flow Cytometry: VE-Cadherin Antibody (PSH03-15) [NBP3-33088] - Flow cytometric analysis of EA.hy926 cells labeling VE-Cadherin. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-33088, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33088_rabbit-ve-cadherin-mab-psh03-15-2920241645321.jpg)
![Immunohistochemistry-Paraffin: P2Y1/P2RY1 Antibody [NBP1-82439] - Staining of human stomach shows low expression as expected.](http://images.novusbio.com/fullsize/P2Y1-P2RY1-Antibody-Immunohistochemistry-Paraffin-NBP1-82439-img0008.jpg "Immunohistochemistry-Paraffin: P2Y1/P2RY1 Antibody [NBP1-82439] - Staining of human stomach shows low expression as expected.")
![Immunohistochemistry-Paraffin: P2Y1/P2RY1 Antibody [NBP1-82439] - Staining of human placenta shows high expression.](http://images.novusbio.com/fullsize/P2Y1-P2RY1-Antibody-Immunohistochemistry-Paraffin-NBP1-82439-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: P2Y1/P2RY1 Antibody [NBP1-82439] - Staining in human placenta and stomach tissues using anti-P2RY1 antibody. Corresponding P2RY1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/P2Y1-P2RY1-Antibody-Immunohistochemistry-Paraffin-NBP1-82439-img0009.jpg)
![Immunohistochemistry-Paraffin: P2Y8 Antibody [NBP1-87375] - Staining in human lymph node and cerebral cortex tissues using anti-P2RY8 antibody. Corresponding P2RY8 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/P2Y8-Antibody-Immunohistochemistry-Paraffin-NBP1-87375-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: P2Y8 Antibody [NBP1-87375] - Staining in human lymph node and cerebral cortex tissues using anti-P2RY8 antibody. Corresponding P2RY8 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: P2Y8 Antibody [NBP1-87375] - Staining of human lymph node shows high expression.](http://images.novusbio.com/fullsize/P2Y8-Antibody-Immunohistochemistry-Paraffin-NBP1-87375-img0006.jpg)
![Immunohistochemistry-Paraffin: P2Y8 Antibody [NBP1-87375] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/P2Y8-Antibody-Immunohistochemistry-Paraffin-NBP1-87375-img0005.jpg)