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![Western Blot: Aurora A [p Thr288] Antibody [NBP3-05434] - Western blot analysis of extracts of HeLa and HT-29 cells, using Aurora A antibody (NBP3-05434) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours. Hela cells were treated by Nocodazole (50ng/mL) for 20 hours.HT-29 cells were treated by Hydroxyurea (4 mM) for 20 hours or treated by Nocodazole (100ng/mL) for 16 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Aurora-A-[p-Thr288]-Antibody-Western-Blot-NBP3-05434-img0001.jpg "Western Blot: Aurora A [p Thr288] Antibody [NBP3-05434] - Western blot analysis of extracts of HeLa and HT-29 cells, using Aurora A antibody (NBP3-05434) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours. Hela cells were treated by Nocodazole (50ng/mL) for 20 hours.HT-29 cells were treated by Hydroxyurea (4 mM) for 20 hours or treated by Nocodazole (100ng/mL) for 16 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.")
![Simple Western: Aurora A [p Thr288] Antibody - BSA Free [NBP3-05434] - Analysis of protein expression post-drug treatment. (A) CHLA-10 and (B) TC-71 cells treated with drugs were assessed for changes in protein expression 24 h post-treatment via capillary electrophoresis-based Wes analysis. Increased protein levels of KIF11, p-KIF11Thr926 AURKA, and p-AURKAThr288 were observed for the drug combination group, whereas KIF15 levels were noticeably lower. Similarly, enhanced cleaved-PARP expression was observed with the combination treatment. The uncropped blots are shown in Figures S8 and S9. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/37894278 ), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp3-05434_rabbit-polyclonal-aurora-a-p-thr288-antibody-308202410445511.jpg)
![Western Blot: MARCKS [p Ser162] Antibody [NBP3-05423] - Western blot analysis of extracts from 293 cells, using MARCKS antibody (NBP3-05423). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.](http://images.novusbio.com/fullsize/MARCKS [p Ser162] Antibody-Western Blot-NBP3-05423-img0002.jpg "Western Blot: MARCKS [p Ser162] Antibody [NBP3-05423] - Western blot analysis of extracts from 293 cells, using MARCKS antibody (NBP3-05423). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.")
![Immunocytochemistry/Immunofluorescence: MARCKS [p Ser162] Antibody [NBP3-05423] - Immunofluorescence analysis of methanol-fixed HeLa cells using MARCKS antibody (NBP3-05423).](http://images.novusbio.com/fullsize/MARCKS-[p-Ser162]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05423-img0001.jpg)
![Western Blot: CDK2 Antibody (10E0N5) [NBP3-33184] - Western blot analysis of various lysates using CDK2 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-33184_rabbit-cdk2-mab-10e0n5-912202414154382.jpg "Western Blot: CDK2 Antibody (10E0N5) [NBP3-33184] - Western blot analysis of various lysates using CDK2 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.")
![<b>Genetic Strategies Validation. </b>Western Blot: CDK2 Antibody (10E0N5) [NBP3-33184] - Western blot analysis of lysates from wild type(WT) and CDK2 knockout (KO) 293T cells, using [KO Validated] CDK2 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-33184_rabbit-cdk2-mab-10e0n5-822025209873.jpg)
![Western Blot: AKT1/2/3 [p Ser473] Antibody (2E3A3) [NBP3-16948] - Analysis of extracts of various cell lines, using Phospho-Akt-S473 antibody (NBP3-16948) at 1:1000 dilution.Jurkat cells and C6 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/AKT1-2-3-p-Ser473-Antibody-2E3A3-Western-Blot-NBP3-16948-img0001.jpg "Western Blot: AKT1/2/3 [p Ser473] Antibody (2E3A3) [NBP3-16948] - Analysis of extracts of various cell lines, using Phospho-Akt-S473 antibody (NBP3-16948) at 1:1000 dilution.Jurkat cells and C6 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: AKT1/2/3 [p Ser473] Antibody (2E3A3) [NBP3-16948] - Analysis of extracts of NIH/3T3 cells, using Phospho-Akt-S473 antibody (NBP3-16948) at 1:1000 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/AKT1-2-3-p-Ser473-Antibody-2E3A3-Western-Blot-NBP3-16948-img0002.jpg)
![Flow Cytometry: CD5 Antibody (4H8E5) [NBP1-26703] - Analysis using the PE conjugate of NBP1-26703. Double staining of 0.2 ug/1^6 cells from BALB/c spleen with rat anti-mouse CD5-R-PE and rat anti-mouse CD19-FITC Clone 6D5), following which small lymphocytes were gated and analyzed using a flow cytometer.](http://images.novusbio.com/fullsize/CD5-Antibody-4H8E5-Flow-Cytometry-NBP1-26703-img0005.jpg "Flow Cytometry: CD5 Antibody (4H8E5) [NBP1-26703] - Analysis using the PE conjugate of NBP1-26703. Double staining of 0.2 ug/1^6 cells from BALB/c spleen with rat anti-mouse CD5-R-PE and rat anti-mouse CD19-FITC Clone 6D5), following which small lymphocytes were gated and analyzed using a flow cytometer.")
![Flow Cytometry: CD5 Antibody (4H8E5) [NBP1-26703] - Analysis using the Allophycocyanin conjugate of NBP1-26703. Multiple staining of C57BL/6 splenocytes.](http://images.novusbio.com/fullsize/CD5-Antibody-4H8E5-Flow-Cytometry-NBP1-26703-img0002.jpg)
![Flow Cytometry: CD5 Antibody (4H8E5) [NBP1-26703] - Analysis using the Biotin conjugate of NBP1-26703. Multiple staining of C57BL/6 splenocytes.](http://images.novusbio.com/fullsize/CD5-Antibody-4H8E5-Flow-Cytometry-NBP1-26703-img0003.jpg)
![Flow Cytometry: CD5 Antibody (4H8E5) [NBP1-26703] - Analysis using the FITC conjugate of NBP1-26703. Multiple staining of C57BL/6 splenocytes.](http://images.novusbio.com/fullsize/CD5-Antibody-4H8E5-Flow-Cytometry-NBP1-26703-img0004.jpg)
![Western Blot: Aquaporin-2 [p Ser264] Antibody [NBP1-48313] - vasopressin (AVP) treated rat kidney lysate showing specific immunolabeling of the approx 29k and 37k glycosylated form of the AQP2 protein phosphorylated at Ser264.](http://images.novusbio.com/fullsize/Aquaporin-2-[p-Ser264]-Antibody-Western-Blot-NBP1-48313-img0001.jpg "Western Blot: Aquaporin-2 [p Ser264] Antibody [NBP1-48313] - vasopressin (AVP) treated rat kidney lysate showing specific immunolabeling of the approx 29k and 37k glycosylated form of the AQP2 protein phosphorylated at Ser264.")