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![Western Blot: CDC37 [p Ser13] Antibody (5P8B2) [NBP3-33562] - Western blot analysis of lysates from C6 cells, using CDC37 Rabbit mAb at 1:1000 dilution. C6 cells were treated by CIP(20uL/400ul) at 37C for 1 hour.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 3min.](http://images.novusbio.com/fullsize/nbp3-33562_rabbit-cdc37-p-ser13-mab-5p8b2-912202415152871.jpg "Western Blot: CDC37 [p Ser13] Antibody (5P8B2) [NBP3-33562] - Western blot analysis of lysates from C6 cells, using CDC37 Rabbit mAb at 1:1000 dilution. C6 cells were treated by CIP(20uL/400ul) at 37C for 1 hour.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 3min.")
![Western Blot: CDC37 [p Ser13] Antibody (5P8B2) [NBP3-33562] - Western blot analysis of various lysates using CDC37 Rabbit mAb at 1:1000 dilution. Both 293T cells and NIH/3T3 cells were treated by CIP(20uL/400ul) at 37C for 1 hour.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-33562_rabbit-cdc37-p-ser13-mab-5p8b2-912202415111821.jpg)
![Western Blot: AP2M1 Antibody [NBP3-35403] - Western blot analysis of lysates from HeLa cells, using AP2M1 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35403_rabbit-ap2m1-pab-101220241233851.jpg "Western Blot: AP2M1 Antibody [NBP3-35403] - Western blot analysis of lysates from HeLa cells, using AP2M1 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.")
![Western Blot: AP2M1 Antibody [NBP3-35403] - Western blot analysis of various lysates using AP2M1 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35403_rabbit-ap2m1-pab-1012202411552890.jpg)
![Western Blot: GSK-3 alpha/beta [p Ser21] Antibody [NBP3-05443] - Western blot analysis of extracts of 293 cells, using GSK-3 alpha/beta antibody (NBP3-05443) at 1:1000 dilution or GSK3alpha antibody (A0645).293 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-[p-Ser21]-Antibody-Western-Blot-NBP3-05443-img0003.jpg "Western Blot: GSK-3 alpha/beta [p Ser21] Antibody [NBP3-05443] - Western blot analysis of extracts of 293 cells, using GSK-3 alpha/beta antibody (NBP3-05443) at 1:1000 dilution or GSK3alpha antibody (A0645).293 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.")
![Western Blot: GSK-3 alpha/beta [p Ser21] Antibody [NBP3-05443] - Western blot analysis of extracts of 293 and HeLa cells, using GSK-3 alpha/beta antibody (NBP3-05443) at 1:1000 dilution. 293 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. HeLa cells were treated by Insulin (0.01U/mL) for 15 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-[p-Ser21]-Antibody-Western-Blot-NBP3-05443-img0002.jpg)
![Immunoprecipitation: GSK-3 alpha/beta [p Ser21] Antibody [NBP3-05443] - Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug GSK-3 alpha/beta pAb (NBP3-05443). Western blot was performed from the immunoprecipitate using GSK-3 alpha/beta pAb (NBP3-05443) at a dilution of 1:1000. 293T cells were treated by Insulin (100 nM) at 37C for 10 minutes after serum-starvation overnight.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-[p-Ser21]-Antibody-Immunoprecipitation-NBP3-05443-img0001.jpg)
![Western Blot: IRF3 [p Ser396] Antibody [NBP3-05445] - Western blot analysis of extracts of NIH/3T3 cells, using IRF3 antibody (NBP3-05445) at 1:1000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/IRF3-[p-Ser396]-Antibody-Western-Blot-NBP3-05445-img0001.jpg "Western Blot: IRF3 [p Ser396] Antibody [NBP3-05445] - Western blot analysis of extracts of NIH/3T3 cells, using IRF3 antibody (NBP3-05445) at 1:1000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Immunohistochemistry: IRF3 [p Ser396] Antibody - BSA Free [IRF3] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using IRF3 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-05445_rabbit-polyclonal-irf3-p-ser396-antibody-5220251638071.jpg)
![Western Blot: DAP Kinase 1 Antibody [NBP3-38180] - Western blot analysis of various lysates using DAP Kinase 1(DAPK1) Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-38180_rabbit-dap-kinase-1-pab-1012202411353089.jpg "Western Blot: DAP Kinase 1 Antibody [NBP3-38180] - Western blot analysis of various lysates using DAP Kinase 1(DAPK1) Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s.")
![Western Blot: CREB [p Ser129] Antibody [NBP3-05463] - Western blot analysis of extracts of 293T cells, using CREB antibody (NBP3-05463) at 1:500 dilution.293T cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/CREB-[p-Ser129]-Antibody-Western-Blot-NBP3-05463-img0001.jpg "Western Blot: CREB [p Ser129] Antibody [NBP3-05463] - Western blot analysis of extracts of 293T cells, using CREB antibody (NBP3-05463) at 1:500 dilution.293T cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: CREB [p Ser129] Antibody [NBP3-05463] - Western blot analysis of extracts of C6 cells, using CREB antibody (NBP3-05463) at 1:500 dilution.C6 cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/CREB [p Ser129] Antibody-Western Blot-NBP3-05463-img0002.jpg)
![Western Blot: MARCKS [p Ser158] Antibody [NBP3-05422] - Western blot analysis of extracts from 3T3 cells using MARCKS antibody (NBP3-05422). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.](http://images.novusbio.com/fullsize/MARCKS [p Ser158] Antibody-Western Blot-NBP3-05422-img0002.jpg "Western Blot: MARCKS [p Ser158] Antibody [NBP3-05422] - Western blot analysis of extracts from 3T3 cells using MARCKS antibody (NBP3-05422). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.")
![Immunocytochemistry/Immunofluorescence: MARCKS [p Ser158] Antibody [NBP3-05422] - Immunofluorescence analysis of methanol-fixed HeLa cells using MARCKS antibody (NBP3-05422).](http://images.novusbio.com/fullsize/MARCKS-[p-Ser158]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05422-img0001.jpg)
![Western Blot: Aurora A [p Thr288] Antibody [NBP3-05434] - Western blot analysis of extracts of HeLa and HT-29 cells, using Aurora A antibody (NBP3-05434) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours. Hela cells were treated by Nocodazole (50ng/mL) for 20 hours.HT-29 cells were treated by Hydroxyurea (4 mM) for 20 hours or treated by Nocodazole (100ng/mL) for 16 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Aurora-A-[p-Thr288]-Antibody-Western-Blot-NBP3-05434-img0001.jpg "Western Blot: Aurora A [p Thr288] Antibody [NBP3-05434] - Western blot analysis of extracts of HeLa and HT-29 cells, using Aurora A antibody (NBP3-05434) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours. Hela cells were treated by Nocodazole (50ng/mL) for 20 hours.HT-29 cells were treated by Hydroxyurea (4 mM) for 20 hours or treated by Nocodazole (100ng/mL) for 16 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.")
![Simple Western: Aurora A [p Thr288] Antibody - BSA Free [NBP3-05434] - Analysis of protein expression post-drug treatment. (A) CHLA-10 and (B) TC-71 cells treated with drugs were assessed for changes in protein expression 24 h post-treatment via capillary electrophoresis-based Wes analysis. Increased protein levels of KIF11, p-KIF11Thr926 AURKA, and p-AURKAThr288 were observed for the drug combination group, whereas KIF15 levels were noticeably lower. Similarly, enhanced cleaved-PARP expression was observed with the combination treatment. The uncropped blots are shown in Figures S8 and S9. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/37894278 ), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp3-05434_rabbit-polyclonal-aurora-a-p-thr288-antibody-308202410445511.jpg)