| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61776-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61833-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61850-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-160SS | |||
![Western Blot: nNOS Antibody (5K9R2) [NBP3-16008] - Western blot analysis of extracts of Rat brain, using nNOSantibody (NBP3-16008) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/nNOS-Antibody-5K9R2-Western-Blot-NBP3-16008-img0004.jpg "Western Blot: nNOS Antibody (5K9R2) [NBP3-16008] - Western blot analysis of extracts of Rat brain, using nNOSantibody (NBP3-16008) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Immunocytochemistry/ Immunofluorescence: nNOS Antibody (5K9R2) [nNOS] - Confocal imaging of paraffin-embedded Rat brain using nNOS Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16008_rabbit-nnos-mab-5k9r2-52202517213529.jpg)
![Western Blot: GluR2 Antibody (7A7D12) [NBP2-61776] - Analysis using GRIA2 mAb against human GRIA2 (AA: 35-175) recombinant protein. (Expected MW is 41.9 kDa)](http://images.novusbio.com/fullsize/GluR2-Antibody-7A7D12-Western-Blot-NBP2-61776-img0003.jpg "Western Blot: GluR2 Antibody (7A7D12) [NBP2-61776] - Analysis using GRIA2 mAb against human GRIA2 (AA: 35-175) recombinant protein. (Expected MW is 41.9 kDa)")
![Western Blot: GluR2 Antibody (7A7D12) [NBP2-61776] - Analysis using GRIA2 mAb against HEK293 (1) and GRIA2 (AA: 35-175)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/GluR2-Antibody-7A7D12-Western-Blot-NBP2-61776-img0004.jpg)
![ELISA: GluR2 Antibody (7A7D12) [NBP2-61776] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/GluR2-Antibody-7A7D12-ELISA-NBP2-61776-img0001.jpg)
![ELISA: GluR2 Antibody (7A7D12) [NBP2-61776] - Analysis of SK-N-SH cells using GRIA2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/GluR2-Antibody-7A7D12-ELISA-NBP2-61776-img0002.jpg)
![Western Blot: mGluR2 Antibody (4A10B9) [NBP2-61833] - Analysis using GRM2 mAb against human GRM2 (AA: extra 414-558) recombinant protein. (Expected MW is 42.4 kDa)](http://images.novusbio.com/fullsize/mGluR2-Antibody-4A10B9-Western-Blot-NBP2-61833-img0003.jpg "Western Blot: mGluR2 Antibody (4A10B9) [NBP2-61833] - Analysis using GRM2 mAb against human GRM2 (AA: extra 414-558) recombinant protein. (Expected MW is 42.4 kDa)")
![Western Blot: mGluR2 Antibody (4A10B9) [NBP2-61833] - Analysis using GRM2 mAb against HEK293 (1) and GRM2 (AA: extra 414-558)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/mGluR2-Antibody-4A10B9-Western-Blot-NBP2-61833-img0004.jpg)
![ELISA: mGluR2 Antibody (4A10B9) [NBP2-61833] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/mGluR2-Antibody-4A10B9-ELISA-NBP2-61833-img0001.jpg)
![ELISA: mGluR2 Antibody (4A10B9) [NBP2-61833] - Analysis of SK-N-SH cells using GRM2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/mGluR2-Antibody-4A10B9-ELISA-NBP2-61833-img0002.jpg)
![Western Blot: mGluR6 Antibody (7D9C9) [NBP2-61850] - Analysis using GRM6 mAb against human GRM6 (AA: extra 480-585) recombinant protein. (Expected MW is 37.5 kDa)](http://images.novusbio.com/fullsize/mGluR6-Antibody-7D9C9-Western-Blot-NBP2-61850-img0003.jpg "Western Blot: mGluR6 Antibody (7D9C9) [NBP2-61850] - Analysis using GRM6 mAb against human GRM6 (AA: extra 480-585) recombinant protein. (Expected MW is 37.5 kDa)")
![Western Blot: mGluR6 Antibody (7D9C9) [NBP2-61850] - Analysis using GRM6 mAb against HEK293 (1) and GRM6 (AA: extra 480-585)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/mGluR6-Antibody-7D9C9-Western-Blot-NBP2-61850-img0004.jpg)
![ELISA: mGluR6 Antibody (7D9C9) [NBP2-61850] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/mGluR6-Antibody-7D9C9-ELISA-NBP2-61850-img0001.jpg)
![ELISA: mGluR6 Antibody (7D9C9) [NBP2-61850] - Analysis of K562 cells using GRM6 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/mGluR6-Antibody-7D9C9-ELISA-NBP2-61850-img0002.jpg)
![Western Blot: GABA-B R1 Antibody [NB300-160] - Brain lysate with GABA-B R1 antibody at a dilution of 1:500.](http://images.novusbio.com/fullsize/GABA-B-R1-Antibody-Western-Blot-NB300-160-img0003.jpg "Western Blot: GABA-B R1 Antibody [NB300-160] - Brain lysate with GABA-B R1 antibody at a dilution of 1:500.")
![Immunohistochemistry-Paraffin: Nitrotyrosine Antibody (HM11) [NB600-1024] - Analysis using the Biotin conjugate of NB600-1024. Nitrotyrosine in human liver of severely obesed patients. Staining of paraffin tissue section with clone HM.11. Anti-nitrotyrosine at 2 ug/ml (1h, RT).](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-HM11-Immunohistochemistry-Paraffin-NB600-1024-img0003.jpg "Immunohistochemistry-Paraffin: Nitrotyrosine Antibody (HM11) [NB600-1024] - Analysis using the Biotin conjugate of NB600-1024. Nitrotyrosine in human liver of severely obesed patients. Staining of paraffin tissue section with clone HM.11. Anti-nitrotyrosine at 2 ug/ml (1h, RT).")
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (8F1-R) [NBP3-32019] - Western blot analysis of Aldehyde Dehydrogenase 1-A1/ALDH1A1 on different lysates with Mouse anti-Aldehyde Dehydrogenase 1-A1/ALDH1A1 antibody (NBP3-32019) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 ug/Lane) Lane 2: HT-29 cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: K-562 cell lysate (20 ug/Lane) Lane 5: Human liver tissue lysate (40 ug/Lane) Lane 6: Human lung tissue lysate (40 ug/Lane) Predicted band size: 55 kDa Observed band size: 50 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32019) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32019_mouse-aldehyde-dehydrogenase-1-a1-aldh1a1-mab-8f1-r-278202415141717.jpg "Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (8F1-R) [NBP3-32019] - Western blot analysis of Aldehyde Dehydrogenase 1-A1/ALDH1A1 on different lysates with Mouse anti-Aldehyde Dehydrogenase 1-A1/ALDH1A1 antibody (NBP3-32019) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 ug/Lane) Lane 2: HT-29 cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: K-562 cell lysate (20 ug/Lane) Lane 5: Human liver tissue lysate (40 ug/Lane) Lane 6: Human lung tissue lysate (40 ug/Lane) Predicted band size: 55 kDa Observed band size: 50 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32019) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (8F1-R) [NBP3-32019] - Immunocytochemistry analysis of A549 cells labeling Aldehyde Dehydrogenase 1-A1/ALDH1A1 with Mouse anti-Aldehyde Dehydrogenase 1-A1/ALDH1A1 antibody (NBP3-32019) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Aldehyde Dehydrogenase 1-A1/ALDH1A1 antibody (NBP3-32019) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32019_mouse-aldehyde-dehydrogenase-1-a1-aldh1a1-mab-8f1-r-29202410234298.jpg)
![Flow Cytometry: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (8F1-R) [NBP3-32019] - Flow cytometric analysis of A549 cells labeling Aldehyde Dehydrogenase 1-A1/ALDH1A1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32019, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32019_mouse-aldehyde-dehydrogenase-1-a1-aldh1a1-mab-8f1-r-29202416453266.jpg)
![Western Blot: p53 Antibody (6B5-R) [NBP3-32698] - Western blot analysis of p53 on different lysates with Mouse anti-p53 antibody (NBP3-32698) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: MCF7 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 0.5uM doxorubicincell for 24 hours lysate Lane 5: Neuro-2a cell lysate Lane 6: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: Lane 1-2: 1 minute 40 seconds; Lane 3-6: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32698) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32698_mouse-p53-mab-6b5-r-278202415212411.jpg "Western Blot: p53 Antibody (6B5-R) [NBP3-32698] - Western blot analysis of p53 on different lysates with Mouse anti-p53 antibody (NBP3-32698) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: MCF7 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 0.5uM doxorubicincell for 24 hours lysate Lane 5: Neuro-2a cell lysate Lane 6: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: Lane 1-2: 1 minute 40 seconds; Lane 3-6: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32698) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: p53 Antibody (6B5-R) [NBP3-32698] - Immunocytochemistry analysis of A431 cells labeling p53 with Mouse anti-p53 antibody (NBP3-32698) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p53 antibody (NBP3-32698) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32698_mouse-p53-mab-6b5-r-29202410484875.jpg)
![Immunohistochemistry: TRPA1 Antibody [NBP3-10359] - Immunohistochemical analysis of rat sensory ganglia tissue](http://images.novusbio.com/fullsize/TRPA1-Antibody-Immunohistochemistry-NBP3-10359-img0001.jpg "Immunohistochemistry: TRPA1 Antibody [NBP3-10359] - Immunohistochemical analysis of rat sensory ganglia tissue")
![Western Blot: Synaptophysin Antibody (JE42-51) [NBP3-32986] - Western blot analysis of Synaptophysin on different lysates with Rabbit anti-Synaptophysin antibody (NBP3-32986) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse cerebellum tissue lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32986) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32986_rabbit-synaptophysin-mab-je42-51-288202412493471.jpg "Western Blot: Synaptophysin Antibody (JE42-51) [NBP3-32986] - Western blot analysis of Synaptophysin on different lysates with Rabbit anti-Synaptophysin antibody (NBP3-32986) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse cerebellum tissue lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32986) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Synaptophysin Antibody (JE42-51) [NBP3-32986] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Synaptophysin antibody (NBP3-32986) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32986) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32986_rabbit-synaptophysin-mab-je42-51-29820241420922.jpg)
![Immunohistochemistry-Paraffin: OXTR Antibody [NBP2-76347] - Negative Control showing staining of paraffin embedded Human Ovary, with no primary antibody.](http://images.novusbio.com/fullsize/OXTR-Antibody-Immunohistochemistry-Paraffin-NBP2-76347-img0003.jpg "Immunohistochemistry-Paraffin: OXTR Antibody [NBP2-76347] - Negative Control showing staining of paraffin embedded Human Ovary, with no primary antibody.")
![Flow Cytometry: OXTR Antibody [NBP2-76347] - Analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/OXTR-Antibody-Flow-Cytometry-NBP2-76347-img0001.jpg)
![Immunohistochemistry-Paraffin: OXTR Antibody [NBP2-76347] - Staining of paraffin embedded Human Ovary with antibody at 8 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/OXTR-Antibody-Immunohistochemistry-Paraffin-NBP2-76347-img0002.jpg)
![Western Blot: EAAT2/GLT1 Antibody (PS01-62) [NBP3-32287] - Western blot analysis of EAAT2/GLT1 on different lysates with Rabbit anti-EAAT2/GLT1 antibody (NBP3-32287) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate (20 ug/Lane) Lane 2: Rat brain tissue lysate (20 ug/Lane) Lane 3: Mouse cerebellum tissue lysate (20 ug/Lane) Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32287) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32287_rabbit-eaat2-glt1-mab-ps01-62-278202415242637.jpg "Western Blot: EAAT2/GLT1 Antibody (PS01-62) [NBP3-32287] - Western blot analysis of EAAT2/GLT1 on different lysates with Rabbit anti-EAAT2/GLT1 antibody (NBP3-32287) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate (20 ug/Lane) Lane 2: Rat brain tissue lysate (20 ug/Lane) Lane 3: Mouse cerebellum tissue lysate (20 ug/Lane) Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32287) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: EAAT2/GLT1 Antibody (PS01-62) [NBP3-32287] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-EAAT2/GLT1 antibody (NBP3-32287) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32287) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32287_rabbit-eaat2-glt1-mab-ps01-62-29820241435275.jpg)