| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-407SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81016 | |||
| BSA Free | NB100-406SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61679-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61673-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59335-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41301-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37281-0.025ml | |||
![Western Blot: 14-3-3 gamma Antibody (HS23) [NB100-407] - 14-3-3 gamma detected in lysates using NB 100-407. Lane 1: HeLa cell lysate, Lanes 2 and 3: bengamide treated lysates (8h and 24h, respectively).](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-HS23-Western-Blot-NB100-407-img0009.jpg "<b>Biological Strategies Validation. </b>Western Blot: 14-3-3 gamma Antibody (HS23) [NB100-407] - 14-3-3 gamma detected in lysates using NB 100-407. Lane 1: HeLa cell lysate, Lanes 2 and 3: bengamide treated lysates (8h and 24h, respectively).")
![<b>Biological Strategies Validation. </b>Western Blot: 14-3-3 gamma Antibody (HS23) [NB100-407] - Analysis of HeLa (A), A431 (B), NIH3T3 (C) and PC-12 (D) cell lysate using 14-3-3 gamma antibody at 1:500.](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-HS23-Western-Blot-NB100-407-img0008.jpg)
![Western Blot: 14-3-3 gamma [ac Val2] Antibody (KC21) [NB100-406] - (1) HeLa, (2) PC12 and (3) NIH-3T3 whole cell lysates were separated by SDS-PAGE on a 12% gel and transferred to PVDF membrane. The membrane was probed with Anti-14-3-3 gamma [Ac Val2] antibody at 2 ug/ml for 1 hour, followed by incubation a 1:10,000 dilution of anti-rabbit HRP. The protein was detected at approximately 28 kDa after incubation with SuperSignal West Pico Chemiluminescent substrate.](http://images.novusbio.com/fullsize/14-3-3-gamma-ac-Val2-Antibody-KC21-Western-Blot-NB100-406-img0005.jpg "Western Blot: 14-3-3 gamma [ac Val2] Antibody (KC21) [NB100-406] - (1) HeLa, (2) PC12 and (3) NIH-3T3 whole cell lysates were separated by SDS-PAGE on a 12% gel and transferred to PVDF membrane. The membrane was probed with Anti-14-3-3 gamma [Ac Val2] antibody at 2 ug/ml for 1 hour, followed by incubation a 1:10,000 dilution of anti-rabbit HRP. The protein was detected at approximately 28 kDa after incubation with SuperSignal West Pico Chemiluminescent substrate.")
![Immunocytochemistry/Immunofluorescence: 14-3-3 gamma [ac Val2] Antibody (KC21) [NB100-406] - 14-3-3 gamma [ac Val2] antibody was tested in SH-SY5Y cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/14-3-3-gamma-ac-Val2-Antibody-KC21-Immunocytochemistry-Immunofluorescence-NB100-406-img0004.jpg)
![Western Blot: 14-3-3 gamma [ac Val2] Antibody (KC21) [NB100-406] - 14-3-3 gamma detected in lysates using NB100-406. Lane 1: HeLa cell lysates, Lanes 2 and 3: bengamide treated lysates (8h and 24h, respectively).](http://images.novusbio.com/fullsize/14-3-3-gamma-ac-Val2-Antibody-KC21-Western-Blot-NB100-406-img0002.jpg)
![Simple Western: 14-3-3 gamma [ac Val2] Antibody (KC21) [NB100-406] - Lane view shows a specific band for 14-3-3 Gamma in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize/14-3-3-gamma-ac-Val2-Antibody-KC21-Simple-Western-NB100-406-img0007.jpg)
![Western Blot: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) [NBP2-61679] - Analysis using CHRNA6 mAb against human CHRNA6 (AA: 26-239) recombinant protein. (Expected MW is 51.2 kDa)](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-6-CHRNA6-Antibody-5B6G8-Western-Blot-NBP2-61679-img0005.jpg "Western Blot: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) [NBP2-61679] - Analysis using CHRNA6 mAb against human CHRNA6 (AA: 26-239) recombinant protein. (Expected MW is 51.2 kDa)")
![Immunocytochemistry/Immunofluorescence: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) [NBP2-61679] - Analysis of Hela cells using CHRNA6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-6-CHRNA6-Antibody-5B6G8-Immunocytochemistry-Immunofluorescence-NBP2-61679-img0003.jpg)
![Immunohistochemistry: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) [NBP2-61679] - Analysis of paraffin-embedded bladder cancer tissues using CHRNA6 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-6-CHRNA6-Antibody-5B6G8-Immunohistochemistry-NBP2-61679-img0004.jpg)
![Flow Cytometry: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) [NBP2-61679] - Analysis of SK-N-SH cells using CHRNA6 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-6-CHRNA6-Antibody-5B6G8-Flow-Cytometry-NBP2-61679-img0007.jpg)
![Western Blot: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) [NBP2-61679] - Analysis using CHRNA6 mAb against HEK293 (1) and CHRNA6 (AA: 26-239)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-6-CHRNA6-Antibody-5B6G8-Western-Blot-NBP2-61679-img0006.jpg)
![Flow Cytometry: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) [NBP2-61679] - Analysis of SH-SY5Y cells using CHRNA6 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-6-CHRNA6-Antibody-5B6G8-Flow-Cytometry-NBP2-61679-img0002.jpg)
![ELISA: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) [NBP2-61679] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-6-CHRNA6-Antibody-5B6G8-ELISA-NBP2-61679-img0001.jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody [NLS1331] - Brain, Hypothalamus.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M2-CHRM2-Antibody-Immunohistochemistry-Paraffin-NLS1331-img0006.jpg "Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody [NLS1331] - Brain, Hypothalamus.")
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody [NLS1331] - Analysis of anti-CHRM2 / M2 antibody with human brain, neurons and glia.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M2-CHRM2-Antibody-Immunohistochemistry-Paraffin-NLS1331-img0001.jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody [NLS1331] - Analysis of anti-CHRM2 / M2 antibody with human brain, glioblastoma.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M2-CHRM2-Antibody-Immunohistochemistry-Paraffin-NLS1331-img0002.jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody [NLS1331] - Anti-CHRM2 / M2 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M2-CHRM2-Antibody-Immunohistochemistry-Paraffin-NLS1331-img0003.jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody [NLS1331] - Brain, Parkinson's Disease Neuron Lewy BodyBrain, Parkinson's Disease Neuron Lewy Body.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M2-CHRM2-Antibody-Immunohistochemistry-Paraffin-NLS1331-img0005.jpg)
![Western Blot: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis using CHRNA3 mAb against human CHRNA3 (AA: 32-240) recombinant protein. (Expected MW is 50.6 kDa)](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-3-CHRNA3-Antibody-6D3A10-Western-Blot-NBP2-61673-img0005.jpg "Western Blot: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis using CHRNA3 mAb against human CHRNA3 (AA: 32-240) recombinant protein. (Expected MW is 50.6 kDa)")
![Immunocytochemistry/Immunofluorescence: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis of SMMC-7721 cells using CHRNA3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-3-CHRNA3-Antibody-6D3A10-Immunocytochemistry-Immunofluorescence-NBP2-61673-img0004.jpg)
![Flow Cytometry: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis of SK-N-SH cells using CHRNA3 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-3-CHRNA3-Antibody-6D3A10-Flow-Cytometry-NBP2-61673-img0007.jpg)
![Western Blot: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis using CHRNA3 mAb against HEK293 (1) and CHRNA3 (AA: 32-240)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-3-CHRNA3-Antibody-6D3A10-Western-Blot-NBP2-61673-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis of Hela cells using CHRNA3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-3-CHRNA3-Antibody-6D3A10-Immunocytochemistry-Immunofluorescence-NBP2-61673-img0003.jpg)
![Flow Cytometry: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis of SH-SY5Y cells using CHRNA3 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-3-CHRNA3-Antibody-6D3A10-Flow-Cytometry-NBP2-61673-img0002.jpg)
![ELISA: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-3-CHRNA3-Antibody-6D3A10-ELISA-NBP2-61673-img0001.jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody [NLS1333] - Human Ovary, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M2-CHRM2-Antibody-Immunohistochemistry-Paraffin-NLS1333-img0005.jpg "Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody [NLS1333] - Human Ovary, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody [NLS1333] - Analysis of brain, neurons and glia using this antibody at 6 ug/ ml.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M2-CHRM2-Antibody-Immunohistochemistry-Paraffin-NLS1333-img0001.jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody [NLS1333] - Analysis of anti-CHRM2 / M2 antibody with human brain, glioblastoma.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M2-CHRM2-Antibody-Immunohistochemistry-Paraffin-NLS1333-img0002.jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody [NLS1333] - Human Lung, Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M2-CHRM2-Antibody-Immunohistochemistry-Paraffin-NLS1333-img0004.jpg)
![Western Blot: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (3T5W8) [NBP3-16576] - Western blot analysis of extracts of various cell lines, using Muscarinic AChR M2 (Muscarinic Acetylcholine Receptor M2/CHRM2) Rabbit mAb (NBP3-16576) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M2-CHRM2-Antibody-3T5W8-Western-Blot-NBP3-16576-img0004.jpg "Western Blot: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (3T5W8) [NBP3-16576] - Western blot analysis of extracts of various cell lines, using Muscarinic AChR M2 (Muscarinic Acetylcholine Receptor M2/CHRM2) Rabbit mAb (NBP3-16576) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (3T5W8) [NBP3-16576] - Analysis of paraffin-embedded Human esophageal cancer using Muscarinic AChR M2 (Muscarinic AChR M2 (ACM2)) Rabbit mAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-16576_rabbit-muscarinic-acetylcholine-receptor-m2-chrm2-mab-3t5w8-immunohistochemistry-paraffin-3152024111317..jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (3T5W8) [NBP3-16576] - Human tonsil tissue using Muscarinic AChR M2 (ACM2) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16576_rabbit-muscarinic-acetylcholine-receptor-m2-chrm2-mab-3t5w8-immunohistochemistry-paraffin-3152024111413..jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (3T5W8) [NBP3-16576] - Mouse kidney tissue using Muscarinic AChR M2 (ACM2) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16576_rabbit-muscarinic-acetylcholine-receptor-m2-chrm2-mab-3t5w8-immunohistochemistry-paraffin-315202411152..jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (3T5W8) [NBP3-16576] - Mouse testis tissue using Muscarinic AChR M2 (ACM2) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16576_rabbit-muscarinic-acetylcholine-receptor-m2-chrm2-mab-3t5w8-immunohistochemistry-paraffin-315202411165..jpg)
![Immunohistochemistry: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (3T5W8) [NBP3-16576] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Muscarinic AChR M2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16576_rabbit-muscarinic-acetylcholine-receptor-m2-chrm2-mab-3t5w8-52202511402786.jpg)
![Western Blot: GABA-B R2 Antibody (S81-2) [NBP2-59335] - Western Blot analysis of Rat Brain Membrane showing detection of ~105 kDa GABA-B R2 protein using Mouse Anti-GABA-B R2 Monoclonal Antibody, Clone S81-2 (NBP2-59335). Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 ug). . Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-GABA-B R2 Monoclonal Antibody (NBP2-59335) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~105 kDa.](http://images.novusbio.com/fullsize/GABA-B-R2-Antibody-S81-2-Western-Blot-NBP2-59335-img0005.jpg "Western Blot: GABA-B R2 Antibody (S81-2) [NBP2-59335] - Western Blot analysis of Rat Brain Membrane showing detection of ~105 kDa GABA-B R2 protein using Mouse Anti-GABA-B R2 Monoclonal Antibody, Clone S81-2 (NBP2-59335). Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 ug). . Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-GABA-B R2 Monoclonal Antibody (NBP2-59335) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~105 kDa.")
![Immunocytochemistry/Immunofluorescence: GABA-B R2 Antibody (S81-2) [NBP2-59335] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-B Receptor 2 Monoclonal Antibody, Clone S81-2 (NBP2-59335). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-B Receptor 2 Monoclonal Antibody (NBP2-59335) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-B Receptor 2 Antibody (D) Composite.](http://images.novusbio.com/fullsize/GABA-B-R2-Antibody-S81-2-Immunocytochemistry-Immunofluorescence-NBP2-59335-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: GABA-B R2 Antibody (S81-2) [NBP2-59335] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-B Receptor 2 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X.](http://images.novusbio.com/fullsize/GABA-B-R2-Antibody-S81-2-Immunocytochemistry-Immunofluorescence-NBP2-59335-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: GABA-B R2 Antibody (S81-2) [NBP2-59335] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-B Receptor 2 Monoclonal Antibody, Clone S81-2 (NBP2-59335). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-B Receptor 2 Monoclonal Antibody (NBP2-59335) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GABA-B Receptor 2 Antibody. (D) Composite.](http://images.novusbio.com/fullsize/GABA-B-R2-Antibody-S81-2-Immunocytochemistry-Immunofluorescence-NBP2-59335-img0007.jpg)
![Western Blot: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (JE38-35) [NBP3-32617] - Western blot analysis of Muscarinic Acetylcholine Receptor M2/CHRM2 on different lysates with Rabbit anti-Muscarinic Acetylcholine Receptor M2/CHRM2 antibody (NBP3-32617) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: U-87 MG cell lysate Lane 4: Neuro-2a cell lysate Lane 5: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 1 minute 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32617) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32617_rabbit-muscarinic-acetylcholine-receptor-m2-chrm2-mab-27820241631631.jpg "Western Blot: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (JE38-35) [NBP3-32617] - Western blot analysis of Muscarinic Acetylcholine Receptor M2/CHRM2 on different lysates with Rabbit anti-Muscarinic Acetylcholine Receptor M2/CHRM2 antibody (NBP3-32617) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: U-87 MG cell lysate Lane 4: Neuro-2a cell lysate Lane 5: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 1 minute 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32617) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (JE38-35) [NBP3-32617] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Muscarinic Acetylcholine Receptor M2/CHRM2 antibody (NBP3-32617) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32617) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32617_rabbit-muscarinic-acetylcholine-receptor-m2-chrm2-mab-29820241435280.jpg)
![Immunocytochemistry/ Immunofluorescence: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (JE38-35) [NBP3-32617] - Immunocytochemistry analysis of SH-SY5Y cells labeling Muscarinic Acetylcholine Receptor M2/CHRM2 with Rabbit anti-Muscarinic Acetylcholine Receptor M2/CHRM2 antibody (NBP3-32617) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Muscarinic Acetylcholine Receptor M2/CHRM2 antibody (NBP3-32617) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃.Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32617_rabbit-muscarinic-acetylcholine-receptor-m2-chrm2-mab-29202410484825.jpg)
![Flow Cytometry: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (JE38-35) [NBP3-32617] - Flow cytometric analysis of SH-SY5Y cells labeling Muscarinic Acetylcholine Receptor M2/CHRM2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32617, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32617_rabbit-muscarinic-acetylcholine-receptor-m2-chrm2-mab-392024884617.jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (31-1D1) [NB120-2805] - Human kidney tissue.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M2-CHRM2-Antibody-31-1D1-Immunohistochemistry-Paraffin-NB120-2805-img0005.jpg "Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (31-1D1) [NB120-2805] - Human kidney tissue.")
![Western Blot: KCNK1 Antibody [NBP2-41301] - Western blot analysis of KCNK1 in 3T3 cell lysate with KCNK1 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/KCNK1-Antibody-Western-Blot-NBP2-41301-img0001.jpg "Western Blot: KCNK1 Antibody [NBP2-41301] - Western blot analysis of KCNK1 in 3T3 cell lysate with KCNK1 antibody at 1 ug/ml.")
![Immunohistochemistry: KCNK1 Antibody - BSA Free [NBP2-41301] - Immunohistochemistry of KCNK1 in mouse brain tissue with KCNK1 antibody at 5 u/ml.](http://images.novusbio.com/fullsize/nbp2-41301_rabbit-polyclonal-kcnk1-antibody-2711202419541567.jpg)
![Immunocytochemistry/ Immunofluorescence: KCNK1 Antibody - BSA Free [NBP2-41301] - Immunofluorescence of KCNK1 in mouse brain tissue with KCNK1 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41301_rabbit-polyclonal-kcnk1-antibody-2711202419313249.jpg)
![Western Blot: GABA-B R2 Antibody (5G7F7) [NBP2-37281] - Western blot analysis using GABBR2 mAb against HEK293 (1) and GABBR2 (AA: 319-483)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/GABA-B-R2-Antibody-5G7F7-Western-Blot-NBP2-37281-img0003.jpg "Western Blot: GABA-B R2 Antibody (5G7F7) [NBP2-37281] - Western blot analysis using GABBR2 mAb against HEK293 (1) and GABBR2 (AA: 319-483)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: GABA-B R2 Antibody (5G7F7) [NBP2-37281] - Immunofluorescence analysis of Hela cells using GABBR2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/GABA-B-R2-Antibody-5G7F7-Immunocytochemistry-Immunofluorescence-NBP2-37281-img0004.jpg)
![Immunohistochemistry: GABA-B R2 Antibody (5G7F7) [NBP2-37281] - Immunohistochemical analysis of paraffin-embedded cerebellum tissues using GABBR2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/GABA-B-R2-Antibody-5G7F7-Immunohistochemistry-NBP2-37281-img0005.jpg)
![Western Blot: GABA-B R2 Antibody (5G7F7) [NBP2-37281] - Western blot analysis using GABBR2 mAb against human GABBR2 (AA: 319-483) recombinant protein. (Expected MW is 44.9 kDa)](http://images.novusbio.com/fullsize/GABA-B-R2-Antibody-5G7F7-Western-Blot-NBP2-37281-img0002.jpg)
![ELISA: GABA-B R2 Antibody (5G7F7) [NBP2-37281] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/GABA-B-R2-Antibody-5G7F7-ELISA-NBP2-37281-img0001.jpg)