| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48609-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41103-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74848 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03282-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-48318-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12893-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81588-25ul | |||
![Immunohistochemistry-Paraffin: Glut1 Antibody [NBP2-48609] - Immunohistochemical analysis of human placenta and testis tissues. Corresponding SLC2A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Glut1-Antibody-Immunohistochemistry-Paraffin-NBP2-48609-img0017.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Glut1 Antibody [NBP2-48609] - Immunohistochemical analysis of human placenta and testis tissues. Corresponding SLC2A1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Glut1 Antibody [NBP2-48609] - Immunocytochemistry analysis of human cell line U-251 MG shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Glut1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48609-img0018.jpg)
![Immunohistochemistry-Paraffin: Glut1 Antibody [NBP2-48609] - Immunohistochemical analysis of human cerebral cortex shows strong membranous positivity in endothelial cells.](http://images.novusbio.com/fullsize/Glut1-Antibody-Immunohistochemistry-Paraffin-NBP2-48609-img0013.jpg)
![Immunohistochemistry-Paraffin: Glut1 Antibody [NBP2-48609] - Staining of human skin shows moderate membranous positivity in epidermal cells.](http://images.novusbio.com/fullsize/Glut1-Antibody-Immunohistochemistry-NBP2-48609-img0019.jpg)
![Immunohistochemistry: Glut1 Antibody [NBP2-48609] - Immunohistochemistry analysis of mouse brain shows moderate positivity in endothelial cells in the cerebral cortex.](http://images.novusbio.com/fullsize/Glut1-Antibody-Immunohistochemistry-Paraffin-NBP2-48609-img0012.jpg)
![Immunohistochemistry-Paraffin: Glut1 Antibody [NBP2-48609] - Immunohistochemistry analysis of human placenta shows strong membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Glut1-Antibody-Immunohistochemistry-Paraffin-NBP2-48609-img0014.jpg)
![Immunohistochemistry-Paraffin: Glut1 Antibody [NBP2-48609] - Immunohistochemistry analysis of human testis shows no membranous positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/Glut1-Antibody-Immunohistochemistry-Paraffin-NBP2-48609-img0016.jpg)
![Western Blot: IGF-I R/IGF1R Antibody (HL1957) - Azide and BSA Free [NBP3-25519] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with IGF1R antibody [HL1957] (NBP3-25519) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25519_rabbit-igf-i-r-igf1r-mab-hl1957-azide-and-bsa-free-212024936274.jpg "Western Blot: IGF-I R/IGF1R Antibody (HL1957) - Azide and BSA Free [NBP3-25519] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with IGF1R antibody [HL1957] (NBP3-25519) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: IGF-I R/IGF1R Antibody (HL1957) - Azide and BSA Free [NBP3-25519] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with IGF1R antibody [HL1957] (NBP3-25519) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25519_rabbit-igf-i-r-igf1r-mab-hl1957-azide-and-bsa-free-2120249422681.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: IGF-I R/IGF1R Antibody (HL1957) - Azide and BSA Free [NBP3-25519] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with IGF1R antibody [HL1957] (NBP3-25519) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25519_rabbit-igf-i-r-igf1r-mab-hl1957-azide-and-bsa-free-2120249425469.jpg)
![Immunocytochemistry/Immunofluorescence: IGF-I R/IGF1R Antibody (HL1957) - Azide and BSA Free [NBP3-25519] - IGF1R antibody [HL1957] detects IGF1R protein at cell membrane by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IGF1R stained by IGF1R antibody [HL1957] (NBP3-25519) diluted at 1:1000. Blue: Fluoroshield with DAPI . Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp3-25519_rabbit-igf-i-r-igf1r-mab-hl1957-azide-and-bsa-free-2120249425455.jpg)
![Immunohistochemistry-Paraffin: IGF-I R/IGF1R Antibody (HL1957) - Azide and BSA Free [NBP3-25519] - IGF1R antibody [HL1957] detects IGF1R protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. IGF1R stained by IGF1R antibody [HL1957] (NBP3-25519) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25519_rabbit-igf-i-r-igf1r-mab-hl1957-azide-and-bsa-free-2120249422643.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: IGF-I R/IGF1R Antibody (HL1957) - Azide and BSA Free [NBP3-25519] - Wild-type (WT) and IGF1R knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with IGF1R antibody [HL1957] (NBP3-25519) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25519_rabbit-igf-i-r-igf1r-mab-hl1957-azide-and-bsa-free-212024940886.jpg)
![Western Blot: Melanocortin-4 R Antibody [NBP2-41103] - Western blot analysis of MC4R in rat brain tissue lysate with MC4R antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/Melanocortin-4-R-Antibody-Western-Blot-NBP2-41103-img0001.jpg "Western Blot: Melanocortin-4 R Antibody [NBP2-41103] - Western blot analysis of MC4R in rat brain tissue lysate with MC4R antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.")
![Immunohistochemistry-Paraffin: Melanocortin-4 R Antibody [NBP2-41103] - human brain tissue with MC4R antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/Melanocortin-4-R-Antibody-Immunohistochemistry-Paraffin-NBP2-41103-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Melanocortin-4 R Antibody [NBP2-41103] - Human Brain cells with MC4R antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/Melanocortin-4-R-Antibody-Immunofluorescence-NBP2-41103-img0003.jpg)
![Immunohistochemistry: Melanocortin-4 R Antibody - BSA Free [NBP2-41103] - Immunohistochemistry of Melanocortin-4 R in human brain tissue with Melanocortin-4 R antibody at 2.5 u/mL.](http://images.novusbio.com/fullsize/nbp2-41103_rabbit-polyclonal-melanocortin-4-r-antibody-271120242022912.jpg)
![Immunocytochemistry/ Immunofluorescence: Melanocortin-4 R Antibody - BSA Free [NBP2-41103] - Immunofluorescence of Melanocortin-4 R in Human Brain cells with Melanocortin-4 R antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp2-41103_rabbit-polyclonal-melanocortin-4-r-antibody-2711202419562138.jpg)
![Immunohistochemistry: Melanocortin-4 R Antibody - BSA Free [NBP2-41103] - Immunohistochemistry of Melanocortin-4 R in human brain tissue with Melanocortin-4 R antibody at 2.5 u/mL.](http://images.novusbio.com/fullsize/nbp2-41103_rabbit-polyclonal-melanocortin-4-r-antibody-2711202419531688.jpg)
![Western Blot: VIP Antibody (1E8) [NBP2-46349] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VIP.](http://images.novusbio.com/fullsize/VIP-Antibody-1E8-Western-Blot-NBP2-46349-img0008.jpg "Western Blot: VIP Antibody (1E8) [NBP2-46349] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VIP.")
![Immunohistochemistry: VIP Antibody (1E8) [NBP2-46349] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/VIP-Antibody-1E8-Immunohistochemistry-NBP2-46349-img0007.jpg)
![Immunohistochemistry: VIP Antibody (1E8) [NBP2-46349] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/VIP-Antibody-1E8-Immunohistochemistry-NBP2-46349-img0001.jpg)
![Immunohistochemistry: VIP Antibody (1E8) [NBP2-46349] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/VIP-Antibody-1E8-Immunohistochemistry-NBP2-46349-img0002.jpg)
![Immunohistochemistry: VIP Antibody (1E8) [NBP2-46349] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/VIP-Antibody-1E8-Immunohistochemistry-NBP2-46349-img0003.jpg)
![Immunohistochemistry: VIP Antibody (1E8) [NBP2-46349] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/VIP-Antibody-1E8-Immunohistochemistry-NBP2-46349-img0004.jpg)
![Immunohistochemistry: VIP Antibody (1E8) [NBP2-46349] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/VIP-Antibody-1E8-Immunohistochemistry-NBP2-46349-img0005.jpg)
![Immunohistochemistry: VIP Antibody (1E8) [NBP2-46349] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/VIP-Antibody-1E8-Immunohistochemistry-NBP2-46349-img0006.jpg)
![Western Blot: Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody [NBP3-03282] - Analysis of extracts of various cell lines, using Gastrin-releasing Peptide/Bombesin/Neuromedin C antibody (NBP3-03282) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Gastrin-releasing-Peptide-Bombesin-Neuromedin-C-Antibody-Western-Blot-NBP3-03282-img0004.jpg "Western Blot: Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody [NBP3-03282] - Analysis of extracts of various cell lines, using Gastrin-releasing Peptide/Bombesin/Neuromedin C antibody (NBP3-03282) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin: Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody [NBP3-03282] - Mouse heart using Gastrin-releasing Peptide/Bombesin/Neuromedin C Rabbit pAb (NBP3-03282) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Gastrin-releasing-Peptide-Bombesin-Neuromedin-C-Antibody-Immunohistochemistry-Paraffin-NBP3-03282-img0003.jpg)
![Western Blot: Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody [NBP3-03282] - Analysis of extracts of various cell lines, using Gastrin-releasing Peptide/Bombesin/Neuromedin C antibody (NBP3-03282) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Gastrin-releasing Peptide-Bombesin-Neuromedin C Antibody-Western Blot-NBP3-03282-img0005.jpg)
![Immunohistochemistry-Paraffin: Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody [NBP3-03282] - Rat brain using Gastrin-releasing Peptide/Bombesin/Neuromedin C Rabbit pAb (NBP3-03282) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Gastrin-releasing-Peptide-Bombesin-Neuromedin-C-Antibody-Immunohistochemistry-Paraffin-NBP3-03282-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody - BSA Free [Gastrin-releasing Peptide/Bombesin/Neuromedin C] - Immunofluorescence analysis of NIH/3T3 cells using Gastrin-releasing Peptide/Bombesin/Neuromedin C Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03282_rabbit-polyclonal-gastrin-releasing-peptide-bombesin-neuromedin-c-antibody-52202516345228.jpg)
![Immunocytochemistry/ Immunofluorescence: Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody - BSA Free [Gastrin-releasing Peptide/Bombesin/Neuromedin C] - Immunofluorescence analysis of PC-12 cells using Gastrin-releasing Peptide/Bombesin/Neuromedin C Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03282_rabbit-polyclonal-gastrin-releasing-peptide-bombesin-neuromedin-c-antibody-5220251639613.jpg)
![Western Blot: Syntaxin 1A Antibody (2B2F10) [NBP3-15706] - Western blot analysis of extracts of Rat brain, using Syntaxin 1A antibody (NBP3-15706) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/Syntaxin-1A-Antibody-2B2F10-Western-Blot-NBP3-15706-img0004.jpg "Western Blot: Syntaxin 1A Antibody (2B2F10) [NBP3-15706] - Western blot analysis of extracts of Rat brain, using Syntaxin 1A antibody (NBP3-15706) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Western Blot: Syntaxin 1A Antibody (2B2F10) [NBP3-15706] - Western blot analysis of extracts of various cell lines, using Syntaxin 1A antibody (NBP3-15706) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Syntaxin-1A-Antibody-2B2F10-Western-Blot-NBP3-15706-img0005.jpg)
![Immunohistochemistry-Paraffin: Syntaxin 1A Antibody (2B2F10) [NBP3-15706] - Immunohistochemistry of paraffin-embedded mouse brain using Syntaxin 1A Rabbit mAb (NBP3-15706) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Syntaxin-1A-Antibody-2B2F10-Immunohistochemistry-Paraffin-NBP3-15706-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: Syntaxin 1A Antibody (2B2F10) [Syntaxin 1A] - Immunofluorescence analysis of paraffin-embedded rat brain using Syntaxin 1A Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15706_rabbit-syntaxin-1a-mab-2b2f10-522025173456.jpg)
![Immunocytochemistry/ Immunofluorescence: Syntaxin 1A Antibody (2B2F10) [Syntaxin 1A] - Immunofluorescence analysis of paraffin-embedded mouse brain using Syntaxin 1A Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15706_rabbit-syntaxin-1a-mab-2b2f10-522025173352.jpg)
![Western Blot: TRP7 Antibody (N64A/36) [NBP1-48318] - Western Blot analysis of Rat brain membrane lysate showing detection of TrpC7 protein using Mouse Anti-TrpC7 Monoclonal Antibody, Clone N64A/36 (NBP1-48318). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpC7 Monoclonal Antibody (NBP1-48318) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/TRP7-Antibody-N64A-36-Western-Blot-NBP1-48318-img0005.jpg "Western Blot: TRP7 Antibody (N64A/36) [NBP1-48318] - Western Blot analysis of Rat brain membrane lysate showing detection of TrpC7 protein using Mouse Anti-TrpC7 Monoclonal Antibody, Clone N64A/36 (NBP1-48318). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpC7 Monoclonal Antibody (NBP1-48318) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: TRP7 Antibody (N64A/36) [NBP1-48318] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TrpC7 Monoclonal Antibody, Clone N64A/36 (NBP1-48318). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-TrpC7 Monoclonal Antibody (NBP1-48318) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TrpC7 Antibody (D) Composite.](http://images.novusbio.com/fullsize/TRP7-Antibody-N64A-36-Immunocytochemistry-Immunofluorescence-NBP1-48318-img0007.jpg)
![Immunohistochemistry: TRP7 Antibody (N64A/36) [NBP1-48318] - Immunohistochemistry analysis using Mouse Anti-TrpC7 Monoclonal Antibody, Clone N64A/36 (NBP1-48318). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-TrpC7 Monoclonal Antibody (NBP1-48318) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Everything.](http://images.novusbio.com/fullsize/TRP7-Antibody-N64A-36-Immunohistochemistry-NBP1-48318-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: TRP7 Antibody (N64A/36) [NBP1-48318] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-TrpC7 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Nuclear staining .](http://images.novusbio.com/fullsize/TRP7-Antibody-N64A-36-Immunocytochemistry-Immunofluorescence-NBP1-48318-img0004.jpg)
![Immunohistochemistry-Paraffin: TRP7 Antibody (N64A/36) [NBP1-48318] - Mouse backskin sections. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.](http://images.novusbio.com/fullsize/TRP7-Antibody-N64A-36-Immunohistochemistry-Paraffin-NBP1-48318-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Cav1.3 Antibody (S48) [NBP2-12893] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav1.3Monoclonal Antibody, Clone S48 (NBP2-12893). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Cav1.3Monoclonal Antibody (NBP2-12893) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav1.3Antibody (D) Composite.](http://images.novusbio.com/fullsize/Cav1-3-Antibody-S48-Immunocytochemistry-Immunofluorescence-NBP2-12893-img0007.jpg "Immunocytochemistry/Immunofluorescence: Cav1.3 Antibody (S48) [NBP2-12893] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav1.3Monoclonal Antibody, Clone S48 (NBP2-12893). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Cav1.3Monoclonal Antibody (NBP2-12893) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav1.3Antibody (D) Composite.")
![Immunohistochemistry: Cav1.3 Antibody (S48) [NBP2-12893] - Immunohistochemistry analysis using Mouse Anti-CaV1.3 Calcium Channel Monoclonal Antibody, Clone S48 (NBP2-12893). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaV1.3 Calcium Channel Monoclonal Antibody (NBP2-12893) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/Cav1-3-Antibody-S48-Immunohistochemistry-NBP2-12893-img0006.jpg)
![Immunohistochemistry: Cav1.3 Antibody (S48) [NBP2-12893] - IHC analysis of Cav1.3 tested on human hippocampus lysates. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.](http://images.novusbio.com/fullsize/Cav1-3-Antibody-S48-Immunohistochemistry-NBP2-12893-img0001.jpg)
![Immunohistochemistry: Cav1.3 Antibody (S48) [NBP2-12893] - Immunohistochemistry analysis using Mouse Anti-CaV1.3 Calcium Channel Monoclonal Antibody, Clone S48 (NBP2-12893). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaV1.3 Calcium Channel Monoclonal Antibody (NBP2-12893) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/Cav1-3-Antibody-S48-Immunohistochemistry-NBP2-12893-img0005.jpg)
![Western Blot: VIP Antibody (OTI1E8) - Azide and BSA Free [NBP2-74848] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VIP.](http://images.novusbio.com/fullsize/VIP-Antibody-OTI1E8-Azide-and-BSA-Free-Western-Blot-NBP2-74848-img0001.jpg "Western Blot: VIP Antibody (OTI1E8) - Azide and BSA Free [NBP2-74848] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VIP.")
![Immunohistochemistry: VIP Antibody (OTI1E8) - Azide and BSA Free [NBP2-74848] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/VIP-Antibody-OTI1E8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74848-img0007.jpg)
![Immunohistochemistry: VIP Antibody (OTI1E8) - Azide and BSA Free [NBP2-74848] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/VIP-Antibody-OTI1E8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74848-img0002.jpg)
![Immunohistochemistry: VIP Antibody (OTI1E8) - Azide and BSA Free [NBP2-74848] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/VIP-Antibody-OTI1E8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74848-img0003.jpg)
![Immunohistochemistry: VIP Antibody (OTI1E8) - Azide and BSA Free [NBP2-74848] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/VIP-Antibody-OTI1E8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74848-img0004.jpg)
![Immunohistochemistry: VIP Antibody (OTI1E8) - Azide and BSA Free [NBP2-74848] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/VIP-Antibody-OTI1E8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74848-img0005.jpg)
![Immunohistochemistry: VIP Antibody (OTI1E8) - Azide and BSA Free [NBP2-74848] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/VIP-Antibody-OTI1E8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74848-img0006.jpg)
![Immunohistochemistry: VIP Antibody (OTI1E8) - Azide and BSA Free [NBP2-74848] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/VIP-Antibody-OTI1E8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74848-img0008.jpg)
![Immunohistochemistry: Glut1 Antibody (SA0377) [NBP3-32385] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Glut1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32385, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-298202414262648.jpg "Immunohistochemistry: Glut1 Antibody (SA0377) [NBP3-32385] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Glut1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32385, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry: Glut1 Antibody (SA0377) [NBP3-32385] - Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Glut1 with Rabbit anti-Glut1 antibody (NBP3-32385) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32385, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-298202414333665.jpg)
![Immunocytochemistry/ Immunofluorescence: Glut1 Antibody (SA0377) [NBP3-32385] - Immunocytochemistry analysis of HeLa cells labeling Glut1 with Rabbit anti-Glut1 antibody (NBP3-32385) at 1/500 dilution.Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Glucose Transporter GLUT1 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-29202410334092.jpg)
![Flow Cytometry: Glut1 Antibody (SA0377) [NBP3-32385] - Flow cytometric analysis of Jurkat cells labeling Glut1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32385, red) at 1/1,000 dilution, compared with Rabbit IgG Isotype Control (blue). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with an iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-29202416372147.jpg)
![Western Blot: Glut1 Antibody (SA0377) [NBP3-32385] - Western blot analysis of Glut1 on different lysates with Rabbit anti-Glut1 antibody (NBP3-32385) at 1/50,000 dilution. Lane 1: HeLa cell lysate (no heat) Lane 2: HT-29 cell lysate (no heat) Lane 3: HepG2 cell lysate (no heat) Lane 4: NIH/3T3 cell lysate (no heat) Lane 5: L-929 cell lysate (no heat) Lane 6: Mouse brain tissue lysate (no heat) Lane 7: Rat brain tissue lysate (no heat) Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 45-60 kDa Exposure time: Lane 1-7 (left): 20 seconds; Lane 1-7 (right): 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/50,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-3920248453511.jpg)
![Western Blot: beta-3 Adrenergic R/ADRB3 Antibody [H00000155-B01P] - Analysis of ADRB3 expression in transfected 293T cell line by ADRB3 polyclonal antibody. Lane 1: ADRB3 transfected lysate(44.88 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/beta-3-Adrenergic-R-ADRB3-Antibody-Western-Blot-H00000155-B01P-img0005.jpg "Western Blot: beta-3 Adrenergic R/ADRB3 Antibody [H00000155-B01P] - Analysis of ADRB3 expression in transfected 293T cell line by ADRB3 polyclonal antibody. Lane 1: ADRB3 transfected lysate(44.88 KDa). Lane 2: Non-transfected lysate.")
![Flow Cytometry: beta-3 Adrenergic R/ADRB3 Antibody [H00000155-B01P] - Analysis of negative control 293 cells (Black) and ADRB3 expressing 293 cells (Green) using ADRB3 purified MaxPab mouse polyclonal antibody.](http://images.novusbio.com/fullsize/beta-3-Adrenergic-R-ADRB3-Antibody-Flow-Cytometry-H00000155-B01P-img0003.jpg)
![Immunohistochemistry-Paraffin: SYT7 Antibody [NBP1-81588] - Staining in human cerebral cortex and skeletal muscle tissues . Corresponding SYT7 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SYT7-Antibody-Immunohistochemistry-Paraffin-NBP1-81588-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SYT7 Antibody [NBP1-81588] - Staining in human cerebral cortex and skeletal muscle tissues . Corresponding SYT7 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: SYT7 Antibody [NBP1-81588] - Analysis in control (vector only transfected HEK293T lysate) and SYT7 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/SYT7-Antibody-Western-Blot-NBP1-81588-img0015.jpg)
![Western Blot: SYT7 Antibody [NBP1-81588] - Analysis in human cell line SK-BR-3.](http://images.novusbio.com/fullsize/SYT7-Antibody-Western-Blot-NBP1-81588-img0009.jpg)
![Immunohistochemistry-Paraffin: SYT7 Antibody [NBP1-81588] - Staining of human cerebellum shows moderate to strong cytoplasmic positivity in purkinje cells.](http://images.novusbio.com/fullsize/SYT7-Antibody-Immunohistochemistry-Paraffin-NBP1-81588-img0010.jpg)
![Immunohistochemistry-Paraffin: SYT7 Antibody [NBP1-81588] - Staining of human cerebral cortex shows strong positivity in neuropil.](http://images.novusbio.com/fullsize/SYT7-Antibody-Immunohistochemistry-Paraffin-NBP1-81588-img0011.jpg)
![Immunohistochemistry-Paraffin: SYT7 Antibody [NBP1-81588] - Staining of human prostate shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/SYT7-Antibody-Immunohistochemistry-Paraffin-NBP1-81588-img0012.jpg)
![Immunohistochemistry-Paraffin: SYT7 Antibody [NBP1-81588] - Staining of human skeletal muscle shows no cytoplasmic positivity in myocytes as expected.](http://images.novusbio.com/fullsize/SYT7-Antibody-Immunohistochemistry-Paraffin-NBP1-81588-img0013.jpg)