| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43793 | |||
| BSA Free | NBP2-46632-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33498-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-59909SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-46853SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21180-25ul | |||
| Azide and BSA Free | NBP3-44262 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74215 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41264-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92978-0.02ml | |||
![Immunohistochemistry-Paraffin: MBP Antibody (CL2829) [NBP2-46632] - Analysis in human cerebral cortex and liver tissues using NBP2-46632 antibody. Corresponding MBP RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MBP-Antibody-CL2829-Immunohistochemistry-Paraffin-NBP2-46632-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MBP Antibody (CL2829) [NBP2-46632] - Analysis in human cerebral cortex and liver tissues using NBP2-46632 antibody. Corresponding MBP RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: MBP Antibody (2829) [NBP2-46632] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/MBP-Antibody-2829-Western-Blot-NBP2-46632-img0009.jpg)
![Immunohistochemistry-Paraffin: MBP Antibody (CL2829) [NBP2-46632] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/MBP-Antibody-CL2829-Immunohistochemistry-Paraffin-NBP2-46632-img0014.jpg)
![Western Blot: MBP Antibody (2829) [NBP2-46632] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/MBP-Antibody-2829-Western-Blot-NBP2-46632-img0008.jpg)
![Immunohistochemistry: MBP Antibody (2829) [NBP2-46632] - Staining of rat cerebral cortex shows strong immunoreactivity in myelinated fibers.](http://images.novusbio.com/fullsize/MBP-Antibody-2829-Immunohistochemistry-NBP2-46632-img0001.jpg)
![Immunohistochemistry: MBP Antibody (2829) [NBP2-46632] - Staining of mouse hippocampal formation shows strong immunoreactivity in myelinated fibers in the dentate gyrus.](http://images.novusbio.com/fullsize/MBP-Antibody-2829-Immunohistochemistry-NBP2-46632-img0002.jpg)
![Immunohistochemistry: MBP Antibody (2829) [NBP2-46632] - Staining of rat basal forebrain shows strong positivity in myelinated fibers.](http://images.novusbio.com/fullsize/MBP-Antibody-2829-Immunohistochemistry-NBP2-46632-img0004.jpg)
![Immunohistochemistry: MBP Antibody (2829) [NBP2-46632] - Staining of mouse cerebellum shows strong immunoreactivity in myelinated fibers.](http://images.novusbio.com/fullsize/MBP-Antibody-2829-Immunohistochemistry-NBP2-46632-img0005.jpg)
![Immunohistochemistry-Paraffin: MBP Antibody (2829) [NBP2-46632] - Staining of human cerebral cortex shows strong positivity in myelinated fibers.](http://images.novusbio.com/fullsize/MBP-Antibody-2829-Immunohistochemistry-Paraffin-NBP2-46632-img0003.jpg)
![Immunohistochemistry-Paraffin: MBP Antibody (CL2829) [NBP2-46632] - Staining of human cerebellum shows strong positivity in myelinated neural fiebrs.](http://images.novusbio.com/fullsize/MBP-Antibody-CL2829-Immunohistochemistry-Paraffin-NBP2-46632-img0012.jpg)
![Immunohistochemistry-Paraffin: MBP Antibody (CL2829) [NBP2-46632] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/MBP-Antibody-CL2829-Immunohistochemistry-Paraffin-NBP2-46632-img0013.jpg)
![Immunohistochemistry-Paraffin: Cathepsin G Antibody [NBP2-33498] - Analysis in human bone marrow and cerebral cortex tissues. Corresponding Cathepsin G RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Cathepsin-G-Antibody-Immunohistochemistry-Paraffin-NBP2-33498-img0004.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cathepsin G Antibody [NBP2-33498] - Analysis in human bone marrow and cerebral cortex tissues. Corresponding Cathepsin G RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cathepsin G Antibody [NBP2-33498] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/Cathepsin-G-Antibody-Immunohistochemistry-Paraffin-NBP2-33498-img0007.jpg)
![Immunohistochemistry-Paraffin: Cathepsin G Antibody [NBP2-33498] - Staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.](http://images.novusbio.com/fullsize/Cathepsin-G-Antibody-Immunohistochemistry-Paraffin-NBP2-33498-img0001.jpg)
![Immunohistochemistry-Paraffin: Cathepsin G Antibody [NBP2-33498] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/Cathepsin-G-Antibody-Immunohistochemistry-Paraffin-NBP2-33498-img0005.jpg)
![Immunohistochemistry-Paraffin: Cathepsin G Antibody [NBP2-33498] - Staining of human rectum shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Cathepsin-G-Antibody-Immunohistochemistry-Paraffin-NBP2-33498-img0006.jpg)
![Western Blot: HMGB1/HMG-1 Antibody [NBP2-27117] - Analysis using HMGB1 antibody. Human ThP1 cell lysate in the (1) absence and (2) presence of immunizing peptide, as well as (3) mouse NIH 3T3 cell lysate were probed using HMGB1 antibody at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-Western-Blot-NBP2-27117-img0008.jpg "Western Blot: HMGB1/HMG-1 Antibody [NBP2-27117] - Analysis using HMGB1 antibody. Human ThP1 cell lysate in the (1) absence and (2) presence of immunizing peptide, as well as (3) mouse NIH 3T3 cell lysate were probed using HMGB1 antibody at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody [NBP2-27117] - Analysis of decalcified, FFPE tissue sections from the paws of mice with collagen-induced arthritis using the HMGB1 antibody. The antibody was used at 5 ug/ml. Data courtesy of Dr Ulf Andersson, Karolinska Institute.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-Immunohistochemistry-Paraffin-NBP2-27117-img0007.jpg)
![Flow Cytometry: HMGB1/HMG-1 Antibody [NBP2-27117] - Intracellular analysis using HMGB1 antibody. Human monocytes were probed using 0.5 ug of HMGB1 antibody (red) and 0.5 ug of isotype control antibody (green). NBP2-27117 and APC secondary this antibody were used for this test.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-Flow-Cytometry-NBP2-27117-img0006.jpg)
![Simple Western: HMGB1/HMG-1 Antibody [NBP2-27117] - Lane view shows a specific band for HMGB1 in 0.5 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-Simple-Western-NBP2-27117-img0005.jpg)
![Western Blot: PGD2 Synthase/PTGDS Antibody [NB110-59909] - Detection of Prostaglandin D Synthase/Lipocalin in human brain lysates (NB820-59177) using NB110-59909.](http://images.novusbio.com/fullsize/PGD2-Synthase-PTGDS-Antibody-Western-Blot-NB110-59909-img0004.jpg "Western Blot: PGD2 Synthase/PTGDS Antibody [NB110-59909] - Detection of Prostaglandin D Synthase/Lipocalin in human brain lysates (NB820-59177) using NB110-59909.")
![Simple Western: PGD2 Synthase/PTGDS Antibody [NB110-59909] - Simple Western lane view shows a specific band for PGD2/Synthase/PTGDS in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/PGD2-Synthase-PTGDS-Antibody-Simple-Western-NB110-59909-img0005.jpg)
![Western Blot: RCAN1 Antibody [NBP1-46853] - Analysis of RCAN in human fetal brain](http://images.novusbio.com/fullsize/RCAN1-Antibody-Western-Blot-NBP1-46853-img0003.jpg "Western Blot: RCAN1 Antibody [NBP1-46853] - Analysis of RCAN in human fetal brain")
![Immunohistochemistry: RCAN1 Antibody [NBP1-46853] - Analysis of RCAN1 in human fetal brain](http://images.novusbio.com/fullsize/RCAN1-Antibody-Immunohistochemistry-NBP1-46853-img0004.jpg)
![Western Blot: Galectin-3 Antibody [NBP2-16590] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16590-img0007.jpg "Western Blot: Galectin-3 Antibody [NBP2-16590] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Galectin-3 Antibody [NBP2-16590] - Immunohistochemical analysis of paraffin-embedded Gastric carcinoma, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Immunohistochemistry-Paraffin-NBP2-16590-img0003.jpg)
![Western Blot: Galectin-3 Antibody [NBP2-16590] - Sample (30 ug of whole cell lysate) A: A549 B: HCT116 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16590-img0001.jpg)
![Western Blot: Galectin-3 Antibody [NBP2-16590] - Sample (30 ug of whole cell lysate) A: Non-transfected 293T lysate B: LGALS3 transfected 293T lysates 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16590-img0002.jpg)
![Western Blot: Galectin-3 Antibody [NBP2-16590] - A. 50 ug rat colon lysate/extract 12 % SDS-PAGE Galectin3 antibody dilution: 1:1000](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16590-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Galectin-3 Antibody [NBP2-16590] - Wild-type (WT) and Galectin 3 knownout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16590-img0005.jpg)
![Sandwich ELISA: Galectin-3 Antibody [NBP2-16590] - Sandwich ELISA detection of recombinant full-length Galectin 3 protein using as detection antibody at concentration of 1 ug/mL. HRP-conjugated rabbit IgG antibody was diluted at 1:10000 and used to detect the primary antibody.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Sandwich-ELISA-NBP2-16590-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: IP3R1 Antibody (HL1541) - Azide and BSA Free [NBP3-25535] - IP3 Receptor I antibody [HL1541] detects IP3 Receptor I protein at endoplasmic reticulum by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IP3 Receptor I stained by IP3 Receptor I antibody [HL1541] (NBP3-25535) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25535_rabbit-ip3r1-mab-hl1541-azide-and-bsa-free-212024940874.jpg "Immunocytochemistry/Immunofluorescence: IP3R1 Antibody (HL1541) - Azide and BSA Free [NBP3-25535] - IP3 Receptor I antibody [HL1541] detects IP3 Receptor I protein at endoplasmic reticulum by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IP3 Receptor I stained by IP3 Receptor I antibody [HL1541] (NBP3-25535) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Western Blot: IP3R1 Antibody (HL1541) - Azide and BSA Free [NBP3-25535] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with IP3 Receptor I antibody [HL1541] (NBP3-25535) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25535_rabbit-ip3r1-mab-hl1541-azide-and-bsa-free-2120249371512.jpg)
![Western Blot: IP3R1 Antibody (HL1541) - Azide and BSA Free [NBP3-25535] - Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with IP3 Receptor I antibody [HL1541] (NBP3-25535) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25535_rabbit-ip3r1-mab-hl1541-azide-and-bsa-free-2120249432830.jpg)
![Western Blot: IP3R1 Antibody (HL1541) - Azide and BSA Free [NBP3-25535] - Whole cell extract (30 Aug) was separated by 5% SDS-PAGE, and the membrane was blotted with IP3 Receptor I antibody [HL1541] (NBP3-25535) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25535_rabbit-ip3r1-mab-hl1541-azide-and-bsa-free-2120249371526.jpg)
![Immunohistochemistry-Paraffin: IP3R1 Antibody (HL1541) - Azide and BSA Free [NBP3-25535] - IP3 Receptor I antibody [HL1541] detects IP3 Receptor I protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. IP3 Receptor I stained by IP3 Receptor I antibody [HL1541] (NBP3-25535) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25535_rabbit-ip3r1-mab-hl1541-azide-and-bsa-free-2120249425457.jpg)
![Immunohistochemistry-Paraffin: IP3R1 Antibody (HL1541) - Azide and BSA Free [NBP3-25535] - IP3 Receptor I antibody [HL1541] detects IP3 Receptor I protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. IP3 Receptor I stained by IP3 Receptor I antibody [HL1541] (NBP3-25535) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25535_rabbit-ip3r1-mab-hl1541-azide-and-bsa-free-2120249362765.jpg)
![Immunohistochemistry-Paraffin: IP3R1 Antibody (HL1541) - Azide and BSA Free [NBP3-25535] - IP3 Receptor I antibody [HL1541] detects IP3 Receptor I protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. IP3 Receptor I stained by IP3 Receptor I antibody [HL1541] (NBP3-25535) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25535_rabbit-ip3r1-mab-hl1541-azide-and-bsa-free-212024940889.jpg)
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with IP3R1 antibody [HL1541] (NBP3-25535) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25535_rabbit-ip3r1-mab-hl1541-azide-and-bsa-free-western-blot-141120251844343.jpg)
![Western Blot: SLC7A8 Antibody (7D6) [NBP1-47989] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLC7A8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLC7A8.](http://images.novusbio.com/fullsize/SLC7A8-Antibody-7D6-Western-Blot-NBP1-47989-img0001.jpg "Western Blot: SLC7A8 Antibody (7D6) [NBP1-47989] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLC7A8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLC7A8.")
![Immunohistochemistry-Paraffin: SLC7A8 Antibody (7D6) [NBP1-47989] - Carcinoma of bladder tissue.](http://images.novusbio.com/fullsize/SLC7A8-Antibody-7D6-Immunohistochemistry-Paraffin-NBP1-47989-img0012.jpg)
![Immunohistochemistry-Paraffin: SLC7A8 Antibody (7D6) [NBP1-47989] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-SLC7A8 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SLC7A8-Antibody-7D6-Immunohistochemistry-Paraffin-NBP1-47989-img0002.jpg)
![Immunohistochemistry-Paraffin: SLC7A8 Antibody (7D6) [NBP1-47989] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-SLC7A8 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SLC7A8-Antibody-7D6-Immunohistochemistry-Paraffin-NBP1-47989-img0003.jpg)
![Immunohistochemistry-Paraffin: SLC7A8 Antibody (7D6) [NBP1-47989] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-SLC7A8 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SLC7A8-Antibody-7D6-Immunohistochemistry-Paraffin-NBP1-47989-img0004.jpg)
![Immunohistochemistry-Paraffin: SLC7A8 Antibody (7D6) [NBP1-47989] - Staining of paraffin-embedded Carcinoma of bladder tissue using anti-SLC7A8 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SLC7A8-Antibody-7D6-Immunohistochemistry-Paraffin-NBP1-47989-img0005.jpg)
![Immunohistochemistry-Paraffin: SLC7A8 Antibody (7D6) [NBP1-47989] - Staining of paraffin-embedded Carcinoma of thyroid tissue using anti-SLC7A8 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SLC7A8-Antibody-7D6-Immunohistochemistry-Paraffin-NBP1-47989-img0006.jpg)
![Immunohistochemistry-Paraffin: SLC7A8 Antibody (7D6) [NBP1-47989] - Staining of paraffin-embedded colon tissue using anti-SLC7A8 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SLC7A8-Antibody-7D6-Immunohistochemistry-Paraffin-NBP1-47989-img0007.jpg)
![Immunohistochemistry-Paraffin: SLC7A8 Antibody (7D6) [NBP1-47989] - Staining of paraffin-embedded endometrium tissue using anti-SLC7A8 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SLC7A8-Antibody-7D6-Immunohistochemistry-Paraffin-NBP1-47989-img0008.jpg)
![Immunohistochemistry-Paraffin: SLC7A8 Antibody (7D6) [NBP1-47989] - Staining of paraffin-embedded Ovary tissue using anti-SLC7A8mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SLC7A8-Antibody-7D6-Immunohistochemistry-Paraffin-NBP1-47989-img0009.jpg)
![Immunohistochemistry-Paraffin: SLC7A8 Antibody (7D6) [NBP1-47989] - Staining of paraffin-embedded pancreas tissue using anti-SLC7A8 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SLC7A8-Antibody-7D6-Immunohistochemistry-Paraffin-NBP1-47989-img0010.jpg)
![Immunohistochemistry-Paraffin: SLC7A8 Antibody (7D6) [NBP1-47989] Staining of paraffin-embedded prostate tissue using anti-SLC7A8 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SLC7A8-Antibody-7D6-Immunohistochemistry-Paraffin-NBP1-47989-img0011.jpg)
![Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - KO Validation in HeLa Cells.Loading: 10 u of HeLa WT cell lysates or HMGB1/HMG-1 KO cell lysates. Antibodies: HMGB1/HMG-1 (0.5 u/mL) and beta-actin (1 u/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp2-41264_rabbit-polyclonal-hmgb1-hmg-1-antibody-271120241932699.gif "<b>Genetic Strategies Validation. </b>Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - KO Validation in HeLa Cells.Loading: 10 u of HeLa WT cell lysates or HMGB1/HMG-1 KO cell lysates. Antibodies: HMGB1/HMG-1 (0.5 u/mL) and beta-actin (1 u/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Genetic Strategies Validation. </b>Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Validation in Mouse Tissues.Loading: 15 ul of lysate Antibodies: HMGB1/HMG-1 , 1 ug/mL , 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp2-41264_rabbit-polyclonal-hmgb1-hmg-1-antibody-2711202420405564.gif)
![Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Validation in Rat Tissues.Loading: 15 ug of lysate Antibodies: HMGB1/HMG-1, 1 u/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp2-41264_rabbit-polyclonal-hmgb1-hmg-1-antibody-271120242074672.gif)
![Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Validation in Human, Mouse and Rat Cell Lines.Loading: 15 ug of lysate Antibodies: HMGB1/HMG-1, 1 u/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp2-41264_rabbit-polyclonal-hmgb1-hmg-1-antibody-2711202420392765.gif)
![Immunocytochemistry/ Immunofluorescence: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Validation of HMGB1/HMG-1 in HL60 Cells.Immunofluorescent analysis of 4% paraformaldehyde-fixed HL60 cells labeling HMGB1/HMG-1 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).](http://images.novusbio.com/fullsize/nbp2-41264_rabbit-polyclonal-hmgb1-hmg-1-antibody-2711202419432287.jpg)
![Immunohistochemistry: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Validation of HMGB1/HMG-1 in Human Stomach Tissue.Immunohistochemical analysis of paraffin-embedded human stomach tissue using anti-HMGB1/HMG-1 antibody at 1 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp2-41264_rabbit-polyclonal-hmgb1-hmg-1-antibody-2711202420312979.jpg)
![Immunohistochemistry: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Validation of HMGB1/HMG-1 in Mouse Spleen Tissue.Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-HMGB1/HMG-1 antibody at 2 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp2-41264_rabbit-polyclonal-hmgb1-hmg-1-antibody-2711202420392762.jpg)
![Immunohistochemistry: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Validation of HMGB1/HMG-1 in Rat Spleen Tissue. Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-HMGB1/HMG-1 antibody at 1 ul/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp2-41264_rabbit-polyclonal-hmgb1-hmg-1-antibody-2711202420333866.jpg)
![Western Blot: CD11b Antibody [NBP2-92978] - Analysis of extracts of various cell lines, using CD11b antibody (NBP2-92978) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/CD11b Antibody-Western Blot-NBP2-92978-img0005.jpg "Western Blot: CD11b Antibody [NBP2-92978] - Analysis of extracts of various cell lines, using CD11b antibody (NBP2-92978) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.")
![Immunohistochemistry-Paraffin: CD11b Antibody [NBP2-92978] - Mouse spleen using CD11b antibody (NBP2-92978) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CD11b-Antibody-Immunohistochemistry-Paraffin-NBP2-92978-img0004.jpg)
![Immunohistochemistry-Paraffin: CD11b Antibody [NBP2-92978] - Human tonsil using CD11b antibody (NBP2-92978) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CD11b-Antibody-Immunohistochemistry-Paraffin-NBP2-92978-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: CD11b Antibody [NBP2-92978] - Immunofluorescence analysis of TF-1 cells using CD11b Rabbit pAb (NBP2-92978) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CD11b-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92978-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: CD11b Antibody - Azide and BSA Free [NBP2-92978] - Immunofluorescence analysis of TF-1 cells using CD11b Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92978_rabbit-polyclonal-cd11b-antibody-4220251429043.jpg)
![Immunocytochemistry/ Immunofluorescence: CD11b Antibody - Azide and BSA Free [NBP2-92978] - Immunofluorescence analysis of THP-1 cells using CD11b Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92978_rabbit-polyclonal-cd11b-antibody-4220251430438.jpg)
![Immunocytochemistry/ Immunofluorescence: CD11b Antibody - Azide and BSA Free [NBP2-92978] - Immunofluorescence analysis of THP-1 cells using CD11b Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92978_rabbit-polyclonal-cd11b-antibody-4220251429086.jpg)
![Immunocytochemistry/ Immunofluorescence: CD11b Antibody - Azide and BSA Free [NBP2-92978] - Immunofluorescence analysis of THP-1 cells using CD11b Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92978_rabbit-polyclonal-cd11b-antibody-4220251427526.jpg)
![Immunocytochemistry/ Immunofluorescence: CD11b Antibody - Azide and BSA Free [NBP2-92978] - Immunofluorescence analysis of TF-1 cells using CD11b Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92978_rabbit-polyclonal-cd11b-antibody-42202514251860.jpg)
![Western Blot: CaMKIV Antibody (1G4P4) [NBP3-16802] - Western blot analysis of extracts of various cell lines, using CaMKIV antibody (NBP3-16802) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/CaMKIV-Antibody-1G4P4-Western-Blot-NBP3-16802-img0006.jpg "Western Blot: CaMKIV Antibody (1G4P4) [NBP3-16802] - Western blot analysis of extracts of various cell lines, using CaMKIV antibody (NBP3-16802) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: CaMKIV Antibody (1G4P4) [NBP3-16802] - Immunofluorescence analysis of U-2 OS cells using CaMKIV Rabbit mAb (NBP3-16802) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CaMKIV-Antibody-1G4P4-Immunocytochemistry-Immunofluorescence-NBP3-16802-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: CaMKIV Antibody (1G4P4) [NBP3-16802] - Immunofluorescence analysis of NIH-3T3 cells using CaMKIV Rabbit mAb (NBP3-16802) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CaMKIV-Antibody-1G4P4-Immunocytochemistry-Immunofluorescence-NBP3-16802-img0004.jpg)
![Immunohistochemistry: CaMKIV Antibody (1G4P4) [NBP3-16802] - Immunohistochemistry analysis of CaMKIV in paraffin-embedded rat testis tissue using CaMKIV Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16802_rabbit-camkiv-mab-1g4p4-822025208415.jpg)
![Immunohistochemistry: CaMKIV Antibody (1G4P4) [NBP3-16802] - Immunohistochemistry analysis of CaMKIV in paraffin-embedded rat brain tissue using CaMKIV Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16802_rabbit-camkiv-mab-1g4p4-822025208448.jpg)
![Immunohistochemistry: CaMKIV Antibody (1G4P4) [NBP3-16802] - Immunohistochemistry analysis of CaMKIV in paraffin-embedded rat kidney tissue using CaMKIV Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16802_rabbit-camkiv-mab-1g4p4-8220252010484.jpg)
![Immunohistochemistry: CaMKIV Antibody (1G4P4) [NBP3-16802] - Immunohistochemistry analysis of CaMKIV in paraffin-embedded mouse colon tissue using CaMKIV Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16802_rabbit-camkiv-mab-1g4p4-822025208485.jpg)
![Immunohistochemistry: CaMKIV Antibody (1G4P4) [NBP3-16802] - Immunohistochemistry analysis of CaMKIV in paraffin-embedded mouse brain tissue using CaMKIV Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16802_rabbit-camkiv-mab-1g4p4-8220252010477.jpg)
![Immunohistochemistry: CaMKIV Antibody (1G4P4) [NBP3-16802] - Immunohistochemistry analysis of CaMKIV in paraffin-embedded rat colon tissue using CaMKIV Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16802_rabbit-camkiv-mab-1g4p4-822025208456.jpg)
![Immunohistochemistry: CaMKIV Antibody (1G4P4) [NBP3-16802] - Immunohistochemistry analysis of CaMKIV in paraffin-embedded human breast cancer tissue using CaMKIV Rabbit mAb at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16802_rabbit-camkiv-mab-1g4p4-8220252010475.jpg)