| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95093-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61658-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52454-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-22417-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-11267-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05163-0.025ml | |||
![Western Blot: Sodium Potassium ATPase Beta 1 Antibody [NBP2-95093] - Analysis of extracts of various cell lines, using Sodium Potassium ATPase Beta 1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Beta-1-Antibody-Western-Blot-NBP2-95093-img0004.jpg "Western Blot: Sodium Potassium ATPase Beta 1 Antibody [NBP2-95093] - Analysis of extracts of various cell lines, using Sodium Potassium ATPase Beta 1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.")
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody [NBP2-95093] - Paraffin-embedded mouse stomach using Sodium Potassium ATPase Beta 1 .](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Beta-1-Antibody-Immunohistochemistry-Paraffin-NBP2-95093-img0003.jpg)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody [NBP2-95093] - Paraffin-embedded human colon carcinoma using Sodium Potassium ATPase Beta 1 .](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Beta-1-Antibody-Immunohistochemistry-Paraffin-NBP2-95093-img0001.jpg)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody [NBP2-95093] - Paraffin-embedded human gastric cancer using Sodium Potassium ATPase Beta 1 .](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Beta-1-Antibody-Immunohistochemistry-Paraffin-NBP2-95093-img0002.jpg)
![Western Blot: Sodium Potassium ATPase Beta 1 Antibody - BSA Free [Sodium Potassium ATPase Beta 1] - Western blot analysis of various lysates using Sodium Potassium ATPase Beta 1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp2-95093_rabbit-polyclonal-sodium-potassium-atpase-beta-1-antibody-52202516345269.jpg)
![Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Beta 1 Antibody - BSA Free [Sodium Potassium ATPase Beta 1] - Immunofluorescence analysis of NIH/3T3 cells using Sodium Potassium ATPase Beta 1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95093_rabbit-polyclonal-sodium-potassium-atpase-beta-1-antibody-5220251638037.jpg)
![Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Beta 1 Antibody - BSA Free [Sodium Potassium ATPase Beta 1] - Immunofluorescence analysis of C6 cells using Sodium Potassium ATPase Beta 1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95093_rabbit-polyclonal-sodium-potassium-atpase-beta-1-antibody-5220251628041.jpg)
![Western Blot: RPE65 Antibody (5X1F6) [NBP3-16840] - Analysis of extracts of Rat eye, using RPE65 Rabbit mAb (NBP3-16840) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/RPE65-Antibody-5X1F6-Western-Blot-NBP3-16840-img0005.jpg "Western Blot: RPE65 Antibody (5X1F6) [NBP3-16840] - Analysis of extracts of Rat eye, using RPE65 Rabbit mAb (NBP3-16840) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry: RPE65 Antibody (5X1F6) [NBP3-16840] - Immunofluorescence analysis of mouse eye using RPE65 Rabbit mAb (NBP3-16840) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RPE65-Antibody-5X1F6-Immunohistochemistry-NBP3-16840-img0004.jpg)
![Immunohistochemistry-Paraffin: RPE65 Antibody (5X1F6) [NBP3-16840] - Rat retina using RPE65 Rabbit mAb (NBP3-16840) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RPE65-Antibody-5X1F6-Immunohistochemistry-Paraffin-NBP3-16840-img0001.jpg)
![Immunohistochemistry-Paraffin: RPE65 Antibody (5X1F6) [NBP3-16840] - Mouse retina using RPE65 Rabbit mAb (NBP3-16840) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RPE65-Antibody-5X1F6-Immunohistochemistry-Paraffin-NBP3-16840-img0002.jpg)
![Immunohistochemistry: RPE65 Antibody (5X1F6) [NBP3-16840] - Immunofluorescence analysis of rat eye using RPE65 Rabbit mAb (NBP3-16840) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RPE65-Antibody-5X1F6-Immunohistochemistry-NBP3-16840-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: RPE65 Antibody (5X1F6) [NBP3-16840] - Immunofluorescence analysis of paraffin-embedded rat eye using RPE65 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16840_rabbit-rpe65-mab-5x1f6-52202511361295.jpg)
![Western Blot: Neutrophil Elastase/ELA2 Antibody (6B6B10) [NBP2-61658] - Analysis using ELANE mAb against HEK293 (1) and ELANE (AA: 140-267)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-6B6B10-Western-Blot-NBP2-61658-img0005.jpg "Western Blot: Neutrophil Elastase/ELA2 Antibody (6B6B10) [NBP2-61658] - Analysis using ELANE mAb against HEK293 (1) and ELANE (AA: 140-267)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry: Neutrophil Elastase/ELA2 Antibody (6B6B10) [NBP2-61658] - Analysis of paraffin-embedded stomach cancer tissues using ELANE mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-6B6B10-Immunohistochemistry-NBP2-61658-img0003.jpg)
![Flow Cytometry: Neutrophil Elastase/ELA2 Antibody (6B6B10) [NBP2-61658] - Analysis of Hela cells using ELANE mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-6B6B10-Flow-Cytometry-NBP2-61658-img0002.jpg)
![Western Blot: Neutrophil Elastase/ELA2 Antibody (6B6B10) [NBP2-61658] - Analysis using ELANE mAb against human ELANE (AA: 140-267) recombinant protein. (Expected MW is 39 kDa)](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-6B6B10-Western-Blot-NBP2-61658-img0004.jpg)
![Western Blot: Neutrophil Elastase/ELA2 Antibody (6B6B10) [NBP2-61658] - Analysis using ELANE mouse mAb against U937 (1), SPC-A-1 (2), and COS7 (3) cell lysate.](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-6B6B10-Western-Blot-NBP2-61658-img0006.jpg)
![ELISA: Neutrophil Elastase/ELA2 Antibody (6B6B10) [NBP2-61658] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-6B6B10-ELISA-NBP2-61658-img0001.jpg)
![Western Blot: Gemin 3 Antibody (1C9F6) [NBP2-52454] - Analysis using DDX20 mAb against human DDX20 (AA: 725-824) recombinant protein. (Expected MW is 38.4 kDa)](http://images.novusbio.com/fullsize/Gemin-3-Antibody-1C9F6-Western-Blot-NBP2-52454-img0005.jpg "Western Blot: Gemin 3 Antibody (1C9F6) [NBP2-52454] - Analysis using DDX20 mAb against human DDX20 (AA: 725-824) recombinant protein. (Expected MW is 38.4 kDa)")
![Immunocytochemistry/Immunofluorescence: Gemin 3 Antibody (1C9F6) [NBP2-52454] - Analysis of HeLa cells using DDX20 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/Gemin-3-Antibody-1C9F6-Immunofluorescence-NBP2-52454-img0002.jpg)
![Flow Cytometry: Gemin 3 Antibody (1C9F6) [NBP2-52454] - Analysis of HeLa cells using DDX20 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Gemin-3-Antibody-1C9F6-Flow-Cytometry-NBP2-52454-img0001.jpg)
![Western Blot: Gemin 3 Antibody (1C9F6) [NBP2-52454] - Western blot analysis using DDX20 mouse mAb against Jurkat (1), Hela (2), NTERA-2 (3), HL7702 (4), K562 (5), and C6 (6) cell lysate.](http://images.novusbio.com/fullsize/Gemin-3-Antibody-1C9F6-Western-Blot-NBP2-52454-img0003.jpg)
![Western Blot: Gemin 3 Antibody (1C9F6) [NBP2-52454] - Analysis using DDX20 mAb against HEK293 (1) and DDX20 (AA: 725-824)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Gemin-3-Antibody-1C9F6-Western-Blot-NBP2-52454-img0004.jpg)
![ELISA: Gemin 3 Antibody (1C9F6) [NBP2-52454] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/Gemin-3-Antibody-1C9F6-ELISA-NBP2-52454-img0007.jpg)
![Western Blot: SOD3/EC-SOD Antibody (4GG11G6) [NBP1-22417] - analysis of Human cartilage lysates showing detection of SOD3 protein using Mouse Anti-SOD3 Monoclonal Antibody, Clone 4GG11G6 . Primary Antibody: Mouse Anti-SOD3 Monoclonal Antibody at 1:1000. Left: Control, Middle: Young cartilage, Right: Cartilage sample with osteo-arthritis..](http://images.novusbio.com/fullsize/SOD3-EC-SOD-Antibody-4GG11G6-Western-Blot-NBP1-22417-img0004.jpg "Western Blot: SOD3/EC-SOD Antibody (4GG11G6) [NBP1-22417] - analysis of Human cartilage lysates showing detection of SOD3 protein using Mouse Anti-SOD3 Monoclonal Antibody, Clone 4GG11G6 . Primary Antibody: Mouse Anti-SOD3 Monoclonal Antibody at 1:1000. Left: Control, Middle: Young cartilage, Right: Cartilage sample with osteo-arthritis..")
![Immunohistochemistry: SOD3/EC-SOD Antibody (4GG11G6) [NBP1-22417] - analysis using Mouse Anti-SOD3 Monoclonal Antibody, Clone 4GG11G6 . Tissue: cartilage. Species: Human. Primary Antibody: Mouse Anti-SOD3 Monoclonal Antibody at 1:1000.](http://images.novusbio.com/fullsize/SOD3-EC-SOD-Antibody-4GG11G6-Immunohistochemistry-NBP1-22417-img0003.jpg)
![Western Blot: MAP2 Antibody (HM-2) [NB120-11267] - Rat brain extract (adult and newborn) was separated on SDS-PAGE and probed with Monoclonal PA To MAP2 (MO) CL:HM-2 . The antibody was developed with AlkPhos APA Mouse Fab ads HIgG and a NBTBCIP substrate. Observed molecular weight is 280 kDA.](http://images.novusbio.com/fullsize/MAP2-Antibody-HM-2-Western-Blot-NB120-11267-img0003.jpg "Western Blot: MAP2 Antibody (HM-2) [NB120-11267] - Rat brain extract (adult and newborn) was separated on SDS-PAGE and probed with Monoclonal PA To MAP2 (MO) CL:HM-2 . The antibody was developed with AlkPhos APA Mouse Fab ads HIgG and a NBTBCIP substrate. Observed molecular weight is 280 kDA.")
![Immunocytochemistry/Immunofluorescence: MAP2 Antibody (HM-2) [NB120-11267] - B35 cells.](http://images.novusbio.com/fullsize/MAP2-Antibody-HM-2-Immunocytochemistry-Immunofluorescence-NB120-11267-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: VIP Antibody (2) [NBP1-05163] - Staining in human pancreas tissue. Signal is strong in nerves of exocrine part of pancreas. Dilution: 1:100.](http://images.novusbio.com/fullsize/VIP-Antibody-2-Immunocytochemistry-Immunofluorescence-NBP1-05163-img0005.jpg "Immunocytochemistry/Immunofluorescence: VIP Antibody (2) [NBP1-05163] - Staining in human pancreas tissue. Signal is strong in nerves of exocrine part of pancreas. Dilution: 1:100.")
![Immunohistochemistry-Paraffin: VIP Antibody (2) - BSA Free [NBP1-05163] - Formalin fixed paraffin-embedded human pancreas tissue . Dilution: 1:100](http://images.novusbio.com/fullsize/VIP-Antibody-2-BSA-Free-Immunohistochemistry-Paraffin-NBP1-05163-img0006.jpg)
![Immunohistochemistry-Paraffin: VIP Antibody (2) [NBP1-05163] - Staining of human appendix tissue. Dilution: 1:100](http://images.novusbio.com/fullsize/VIP-Antibody-2-Immunohistochemistry-Paraffin-NBP1-05163-img0002.jpg)
![Immunohistochemistry-Paraffin: VIP Antibody (2) [NBP1-05163] - Staining of human appendix tissue. Dilution: 1:100](http://images.novusbio.com/fullsize/VIP-Antibody-2-Immunohistochemistry-Paraffin-NBP1-05163-img0004.jpg)
![Western Blot: Drebrin 1 Antibody (JE30-20) [NBP3-32279] - Western blot analysis of Drebrin 1 on different lysates with Rabbit anti-Drebrin 1 antibody (NBP3-32279) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Jurkat cell lysate Lane 3: A431 cell lysate (low expression) Lane 4: Mouse brain tissue lysate Lane 5: AD Mouse brain tissue lysate (low expression) Lane 6: Rat brain tissue lysate Lane 7: PC-12 cell lysate Lane 8: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 71 kDa Observed band size: 110 kDa Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32279) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32279_rabbit-drebrin-1-mab-je30-20-278202415242654.jpg "Western Blot: Drebrin 1 Antibody (JE30-20) [NBP3-32279] - Western blot analysis of Drebrin 1 on different lysates with Rabbit anti-Drebrin 1 antibody (NBP3-32279) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Jurkat cell lysate Lane 3: A431 cell lysate (low expression) Lane 4: Mouse brain tissue lysate Lane 5: AD Mouse brain tissue lysate (low expression) Lane 6: Rat brain tissue lysate Lane 7: PC-12 cell lysate Lane 8: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 71 kDa Observed band size: 110 kDa Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32279) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Drebrin 1 Antibody (JE30-20) [NBP3-32279] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Drebrin 1 antibody (NBP3-32279) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32279) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32279_rabbit-drebrin-1-mab-je30-20-29820241435255.jpg)
![Immunocytochemistry/ Immunofluorescence: Drebrin 1 Antibody (JE30-20) [NBP3-32279] - Immunocytochemistry analysis of SH-SY5Y cells labeling Drebrin 1 with Rabbit anti-Drebrin 1 1 antibody (NBP3-32279) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Drebrin 1 antibody (NBP3-32279) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32279_rabbit-drebrin-1-mab-je30-20-29202410484899.jpg)
![Flow Cytometry: Drebrin 1 Antibody (JE30-20) [NBP3-32279] - Flow cytometric analysis of SH-SY5Y cells labeling Drebrin 1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32279, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32279_rabbit-drebrin-1-mab-je30-20-392024884613.jpg)
![Western Blot: RelA/NFkB p65 Antibody (PSH0-27) [NBP3-32648] - Western blot analysis of RelA/NFkB p65 on different lysates with Rabbit anti-RelA/NFkB p65 antibody (NBP3-32648) at 1/500 dilution. Lane 1: Hela cell lysate (control) Lane 2: Hela-RELA-KO (1) Lane 3: Hela-RELA-KO (2) Lysates/proteins at 35 ug/Lane. Predicted band size: 60 kDa Observed band size: 65 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32648) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32648_rabbit-rela-nfkb-p65-mab-psh0-27-278202415212453.jpg "Western Blot: RelA/NFkB p65 Antibody (PSH0-27) [NBP3-32648] - Western blot analysis of RelA/NFkB p65 on different lysates with Rabbit anti-RelA/NFkB p65 antibody (NBP3-32648) at 1/500 dilution. Lane 1: Hela cell lysate (control) Lane 2: Hela-RELA-KO (1) Lane 3: Hela-RELA-KO (2) Lysates/proteins at 35 ug/Lane. Predicted band size: 60 kDa Observed band size: 65 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32648) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: RelA/NFkB p65 Antibody (PSH0-27) [NBP3-32648] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-RelA/NFkB p65 antibody (NBP3-32648) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32648) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32648_rabbit-rela-nfkb-p65-mab-psh0-27-29820241435276.jpg)
![Immunocytochemistry/ Immunofluorescence: RelA/NFkB p65 Antibody (PSH0-27) [NBP3-32648] - Immunocytochemistry analysis of NIH/3T3 cells treated with or without 50μg/mL TNF-alpha for 20 minutes labeling RelA/NFkB p65 with Rabbit anti-RelA/NFkB p65 antibody (NBP3-32648) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RelA/NFkB p65 antibody (NBP3-32648) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32648_rabbit-rela-nfkb-p65-mab-psh0-27-29202410484811.jpg)
![Flow Cytometry: RelA/NFkB p65 Antibody (PSH0-27) [NBP3-32648] - Flow cytometric analysis of NIH/3T3 cells labeling RelA/NFkB p65. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32648, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32648_rabbit-rela-nfkb-p65-mab-psh0-27-2920241637214.jpg)
![Immunohistochemistry-Paraffin: alpha-1D Adrenergic R/ADRA1D Antibody [NLS12] - Vascular smooth muscle](http://images.novusbio.com/fullsize/alpha-1D-Adrenergic-R-ADRA1D-Antibody-Immunohistochemistry-Paraffin-NLS12-img0005.jpg "Immunohistochemistry-Paraffin: alpha-1D Adrenergic R/ADRA1D Antibody [NLS12] - Vascular smooth muscle")
![Immunohistochemistry-Paraffin: alpha-1D Adrenergic R/ADRA1D Antibody [NLS12] - Analysis of anti-ADRA1D antibody with human prostate.](http://images.novusbio.com/fullsize/alpha-1D-Adrenergic-R-ADRA1D-Antibody-Immunohistochemistry-Paraffin-NLS12-img0002.jpg)
![Immunohistochemistry-Paraffin: alpha-1D Adrenergic R/ADRA1D Antibody [NLS12] - Anti-ADRA1D antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/alpha-1D-Adrenergic-R-ADRA1D-Antibody-Immunohistochemistry-Paraffin-NLS12-img0003.jpg)
![Immunohistochemistry-Paraffin: alpha-1D Adrenergic R/ADRA1D Antibody [NLS12] - Brain Thalamus Adjacent Choroid Plexus Epithelium](http://images.novusbio.com/fullsize/alpha-1D-Adrenergic-R-ADRA1D-Antibody-Immunohistochemistry-Paraffin-NLS12-img0004.jpg)
![Western Blot: Cytokeratin 19 Antibody (SA30-06) [NBP3-32248] - All lanes: Western blot analysis of Cytokeratin 19 with anti-Cytokeratin 19 antibody [SA30-06] (NBP3-32248) at 1:5,000 dilution. Lane 1: Wild-type HepG2 whole cell lysate (20 ug). Lane 2/3: Cytokeratin 19 knockout HepG2 whole cell lysate (20 ug). NBP3-32248 was shown to specifically react with Cytokeratin 19 in wild-type HepG2 cells. No band was observed when Cytokeratin 19 knockout samples were tested. Wild-type and Cytokeratin 19 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-Cytokeratin 19 Antibody (ET1601-6, 1/5,000) and anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32248_rabbit-cytokeratin-19-mab-sa30-06-278202416285243.jpg "Western Blot: Cytokeratin 19 Antibody (SA30-06) [NBP3-32248] - All lanes: Western blot analysis of Cytokeratin 19 with anti-Cytokeratin 19 antibody [SA30-06] (NBP3-32248) at 1:5,000 dilution. Lane 1: Wild-type HepG2 whole cell lysate (20 ug). Lane 2/3: Cytokeratin 19 knockout HepG2 whole cell lysate (20 ug). NBP3-32248 was shown to specifically react with Cytokeratin 19 in wild-type HepG2 cells. No band was observed when Cytokeratin 19 knockout samples were tested. Wild-type and Cytokeratin 19 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-Cytokeratin 19 Antibody (ET1601-6, 1/5,000) and anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Cytokeratin 19 Antibody (SA30-06) [NBP3-32248] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Cytokeratin 19 antibody (NBP3-32248) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32248) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32248_rabbit-cytokeratin-19-mab-sa30-06-298202414115431.jpg)
![Immunohistochemistry: Cytokeratin 19 Antibody (SA30-06) [NBP3-32248] - Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling Cytokeratin 19 (NBP3-32248). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 19 (NBP3-32248, red) at 1/500 dilution at +4℃ overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 594) was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32248_rabbit-cytokeratin-19-mab-sa30-06-298202414333689.jpg)
![Immunocytochemistry/ Immunofluorescence: Cytokeratin 19 Antibody (SA30-06) [NBP3-32248] - ICC staining of Cytokeratin 19 in AGS cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (NBP3-32248, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32248_rabbit-cytokeratin-19-mab-sa30-06-29202410484831.jpg)
![Flow Cytometry: Cytokeratin 19 Antibody (SA30-06) [NBP3-32248] - Flow cytometric analysis of Cytokeratin 19 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (NBP3-32248, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).](http://images.novusbio.com/fullsize/nbp3-32248_rabbit-cytokeratin-19-mab-sa30-06-39202488466.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (4C4.9 + S100B/1012) [NBP2-54559] - analysis of A2058 cells using AF488-labeled S100B Monoclonal Antibody (4C4.9 + S100B/1012) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-+-S100B-1012-Immunocytochemistry-Immunofluorescence-NBP2-54559-img0002.jpg "Immunocytochemistry/Immunofluorescence: S100B Antibody (4C4.9 + S100B/1012) [NBP2-54559] - analysis of A2058 cells using AF488-labeled S100B Monoclonal Antibody (4C4.9 + S100B/1012) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue).")
![Immunohistochemistry-Paraffin: S100B Antibody (4C4.9 + S100B/1012) [NBP2-54559] - Formalin-fixed, paraffin-embedded human Melanoma stained with S100B Mouse Monoclonal Antibody (4C4.9 + S100B/1012)](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-+-S100B-1012-Immunohistochemistry-Paraffin-NBP2-54559-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (4C4.9 + S100B/1012) [NBP2-54559] - analysis of A2058 cells using AF488-labeled Isotype Control Monoclonal Antibody (IgG2a) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue). (Negative Control)](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-+-S100B-1012-Immunocytochemistry-Immunofluorescence-NBP2-54559-img0001.jpg)