| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72977 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70166 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73030 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41270-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03282-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92546-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-94458-0.02ml | |||
![Western Blot: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Untreated (–) and treated (+) SK-N-SH whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (p Thr181) antibody [HL2497] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-western-blot-294202517153..jpg "<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Untreated (–) and treated (+) SK-N-SH whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (p Thr181) antibody [HL2497] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Untreated (–) and treated (+) mouse tissue extract (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (phospho Thr181) antibody [HL2497] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-western-blot-30420259378..jpg)
![Western Blot: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Whole cell and rat tissue extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (p Thr181) antibody [HL2497] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-western-blot-304202593920..jpg)
![ELISA: Rabbit Tau [p Thr181] mAb (HL2497) - Sandwich ELISA detection of Tau peptide and Tau (p Thr181) peptide using antibodies as below.Capture: Tau (p Thr181) antibody [HL2498] (NBP3-45158) (5 ug/mL)Detection: HRP-conjugated Tau (p Thr181) antibody [HL2497] (NBP3-45157) (1 ug/mL)Please notice that NBP3-45157 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-45158.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-elisa-304202594949.jpg)
![ELISA: Rabbit Tau [p Thr181] mAb (HL2497) - Sandwich ELISA detection of Tau peptide and Tau (p Thr181) peptide using antibodies as below.Capture: Tau (phospho Thr181) antibody [HL2497] (NBP3-45157) (5 ug/mL)Detection: HRP-conjugated Tau (p Thr181) antibody [HL2498] (NBP3-45158) (1 ug/mL)Please notice that NBP3-45158 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-45157. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-elisa-304202512432..jpg)
![Immunohistochemistry: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Tau (p Thr181) antibody [HL2497] detects Tau (p Thr181) protein at cytoplasm by immunohistochemical analysis in the presence of control peptide (left) or antigen-specific peptide (right).Sample: Paraffin-embedded mouse brain.Tau (p Thr181) stained by Tau (p Thr181) antibody [HL2497] (GTX638855) diluted at 1:100.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-immunohistochemistry-3042025122656..jpg)
![Western Blot: IGF-I R/IGF1R Antibody (HL1957) - Azide and BSA Free [NBP3-25519] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with IGF1R antibody [HL1957] (NBP3-25519) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25519_rabbit-igf-i-r-igf1r-mab-hl1957-azide-and-bsa-free-212024936274.jpg "Western Blot: IGF-I R/IGF1R Antibody (HL1957) - Azide and BSA Free [NBP3-25519] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with IGF1R antibody [HL1957] (NBP3-25519) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: IGF-I R/IGF1R Antibody (HL1957) - Azide and BSA Free [NBP3-25519] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with IGF1R antibody [HL1957] (NBP3-25519) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25519_rabbit-igf-i-r-igf1r-mab-hl1957-azide-and-bsa-free-2120249422681.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: IGF-I R/IGF1R Antibody (HL1957) - Azide and BSA Free [NBP3-25519] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with IGF1R antibody [HL1957] (NBP3-25519) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25519_rabbit-igf-i-r-igf1r-mab-hl1957-azide-and-bsa-free-2120249425469.jpg)
![Immunocytochemistry/Immunofluorescence: IGF-I R/IGF1R Antibody (HL1957) - Azide and BSA Free [NBP3-25519] - IGF1R antibody [HL1957] detects IGF1R protein at cell membrane by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IGF1R stained by IGF1R antibody [HL1957] (NBP3-25519) diluted at 1:1000. Blue: Fluoroshield with DAPI . Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp3-25519_rabbit-igf-i-r-igf1r-mab-hl1957-azide-and-bsa-free-2120249425455.jpg)
![Immunohistochemistry-Paraffin: IGF-I R/IGF1R Antibody (HL1957) - Azide and BSA Free [NBP3-25519] - IGF1R antibody [HL1957] detects IGF1R protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. IGF1R stained by IGF1R antibody [HL1957] (NBP3-25519) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25519_rabbit-igf-i-r-igf1r-mab-hl1957-azide-and-bsa-free-2120249422643.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: IGF-I R/IGF1R Antibody (HL1957) - Azide and BSA Free [NBP3-25519] - Wild-type (WT) and IGF1R knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with IGF1R antibody [HL1957] (NBP3-25519) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25519_rabbit-igf-i-r-igf1r-mab-hl1957-azide-and-bsa-free-212024940886.jpg)
![Western Blot: HCN4 Antibody (SHG 1E5) [NB100-74439] - Analysis of 25 ug of rat eye lysate onto an SDS polyacrylamide gel.](http://images.novusbio.com/fullsize/HCN4-Antibody-SHG-1E5-Western-Blot-NB100-74439-img0005.jpg "Western Blot: HCN4 Antibody (SHG 1E5) [NB100-74439] - Analysis of 25 ug of rat eye lysate onto an SDS polyacrylamide gel.")
![Immunohistochemistry-Paraffin: HCN4 Antibody (SHG 1E5) [NB100-74439] - Both normal and cancer biopsies of deparaffinized Human heart tissue tissues.](http://images.novusbio.com/fullsize/HCN4-Antibody-SHG-1E5-Immunohistochemistry-Paraffin-NB100-74439-img0004.jpg)
![Immunohistochemistry-Paraffin: HCN4 Antibody (SHG 1E5) [NB100-74439] - Both normal and cancer biopsies of deparaffinized Human tonsil tissue tissues.](http://images.novusbio.com/fullsize/HCN4-Antibody-SHG-1E5-Immunohistochemistry-Paraffin-NB100-74439-img0003.jpg)
![Western Blot: NF-M Antibody (2G3) [NBP2-46139] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NF-M.](http://images.novusbio.com/fullsize/NF-M-Antibody-2G3-Western-Blot-NBP2-46139-img0010.jpg "Western Blot: NF-M Antibody (2G3) [NBP2-46139] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NF-M.")
![Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human testicular cancer tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/NF-M-Antibody-2G3-Immunohistochemistry-NBP2-46139-img0009.jpg)
![Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human skin tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/NF-M-Antibody-2G3-Immunohistochemistry-NBP2-46139-img0001.jpg)
![Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human adult heart tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/NF-M-Antibody-2G3-Immunohistochemistry-NBP2-46139-img0002.jpg)
![Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human adult brain tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/NF-M-Antibody-2G3-Immunohistochemistry-NBP2-46139-img0003.jpg)
![Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human melanoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/NF-M-Antibody-2G3-Immunohistochemistry-NBP2-46139-img0004.jpg)
![Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human embryonic liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/NF-M-Antibody-2G3-Immunohistochemistry-NBP2-46139-img0005.jpg)
![Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human muscle tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/NF-M-Antibody-2G3-Immunohistochemistry-NBP2-46139-img0006.jpg)
![Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human embryonic brain cortex tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/NF-M-Antibody-2G3-Immunohistochemistry-NBP2-46139-img0007.jpg)
![Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human embryonic cerebellum tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/NF-M-Antibody-2G3-Immunohistochemistry-NBP2-46139-img0008.jpg)
![NF-M Antibody (OTI2G3)[NBP2-46139]Simple Western� analysis of lysates (0.2 mg/mL) from HEK293T cell culture using CD63 mouse mAb (SKU: TA803474). EVs were harvested from cell culture media and enriched by polymer-based precipitation. EV contained cell culture media without enrichment was used as controls. The virtual lane view (left) shows the target band corresponding to CD63 (highly glycosylated) at 1:20 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary of EV lysate (Green), cell lysate (Blue) and unenriched media (Grey). The experiment was performed on the Jess Simple Western instrument for ProteinSimple, a BioTechne band, using the 12-230 kDa separation module. *CD63 is highly glycosylated protein, verified by overexpressed lysate tagged with DDK. PNGase F treatment can bring the band to the expected size.](http://images.novusbio.com/fullsize/nbp2-46139_mouse-nf-m-mab-oti2g3-simple-western-131120251236356.jpg%253fstore=america&image-type=image)
![Western Blot: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY ALOX5 (RC217259, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiALOX5.(1:2000)](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-3F1-Western-Blot-NBP2-46513-img0003.jpg "Western Blot: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY ALOX5 (RC217259, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiALOX5.(1:2000)")
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - Paraffin-embedded Adenocarcinoma of Human ovary tissue using antiALOX5 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150)](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-3F1-Immunohistochemistry-Paraffin-NBP2-46513-img0014.jpg)
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - Paraffin-embedded Human colon tissue using anti-ALOX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150).](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-3F1-Immunohistochemistry-Paraffin-NBP2-46513-img0005.jpg)
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - Paraffin-embedded Human Kidney tissue using anti-ALOX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,(1:150)](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-3F1-Immunohistochemistry-Paraffin-NBP2-46513-img0007.jpg)
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - Paraffin-embedded Carcinoma of Human kidney tissue using anti-ALOX5 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min (1:150)](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-3F1-Immunohistochemistry-Paraffin-NBP2-46513-img0010.jpg)
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - Paraffin-embedded Human liver tissue using anti-ALOX5 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,1:150).](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-3F1-Immunohistochemistry-Paraffin-NBP2-46513-img0015.jpg)
![Western Blot: Noggin Antibody (OTI1H8) [NBP1-47881] - Noggin Antibody (1H8) [NBP1-47881] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Noggin monoclonal antibody.](http://images.novusbio.com/fullsize/Noggin-Antibody-1H8-Western-Blot-NBP1-47881-img0012.jpg "Western Blot: Noggin Antibody (OTI1H8) [NBP1-47881] - Noggin Antibody (1H8) [NBP1-47881] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Noggin monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: Noggin Antibody (OTI1H8) [NBP1-47881] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Noggin.](http://images.novusbio.com/fullsize/Noggin-Antibody-1H8-Immunocytochemistry-NBP1-47881-img0007.jpg)
![Immunohistochemistry-Paraffin: Noggin Antibody (1H8) [NBP1-47881] - Staining of paraffin-embedded Human Ovary tissue using anti-Noggin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Noggin-Antibody-1H8-Immunohistochemistry-Paraffin-NBP1-47881-img0009.jpg)
![Western Blot: Noggin Antibody (1H8) [NBP1-47881] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Noggin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Noggin.](http://images.novusbio.com/fullsize/Noggin-Antibody-1H8-Western-Blot-NBP1-47881-img0010.jpg)
![Western Blot: Noggin Antibody (1H8) [NBP1-47881] - Noggingin antibody (1H8) at 1:40000 + Recombinant human Noggingin.](http://images.novusbio.com/fullsize/Noggin-Antibody-1H8-Western-Blot-NBP1-47881-img0011.jpg)
![Immunohistochemistry-Paraffin: Noggin Antibody (1H8) [NBP1-47881] - Staining of paraffin-embedded Human lung tissue using anti-Noggin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Noggin-Antibody-1H8-Immunohistochemistry-Paraffin-NBP1-47881-img0008.jpg)
![Western Blot: Brg1 Antibody [NBP2-41270] - Analysis of SMARCA4 in SK-N-SH cell lysate with SMARCA4 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/Brg1-Antibody-Western-Blot-NBP2-41270-img0001.jpg "Western Blot: Brg1 Antibody [NBP2-41270] - Analysis of SMARCA4 in SK-N-SH cell lysate with SMARCA4 antibody at 1 ug/ml.")
![Immunohistochemistry-Paraffin: Brg1 Antibody [NBP2-41270] - Mouse testis tissue with SMARCA4 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Brg1-Antibody-Immunohistochemistry-Paraffin-NBP2-41270-img0005.jpg)
![Immunohistochemistry: Brg1 Antibody [NBP2-41270] - Mouse testis tissue with SMARCA4 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/Brg1-Antibody-Immunofluorescence-NBP2-41270-img0004.jpg)
![Immunohistochemistry: Brg1 Antibody - BSA Free [NBP2-41270] - Immunohistochemistry of Brg1 in rat brain tissue with Brg1 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41270_rabbit-polyclonal-brg1-antibody-2711202420141992.jpg)
![Immunohistochemistry: Brg1 Antibody - BSA Free [NBP2-41270] - Immunohistochemistry of Brg1 in rat brain tissue with Brg1 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41270_rabbit-polyclonal-brg1-antibody-27112024201313.jpg)
![Western Blot: ITM2B Antibody [NBP1-36963] - HepG2 cell lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ITM2B-Antibody-Western-Blot-NBP1-36963-img0006.jpg "Western Blot: ITM2B Antibody [NBP1-36963] - HepG2 cell lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: ITM2B Antibody [NBP1-36963] - Paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary antibody at 10 ug/mL, 1 hr incubation followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi apparatus and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10u g/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/ITM2B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-36963-img0008.jpg)
![Immunohistochemistry-Paraffin: ITM2B Antibody [NBP1-36963] - Human small intestine tissue. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/ITM2B-Antibody-Immunohistochemistry-Paraffin-NBP1-36963-img0003.jpg)
![Flow Cytometry: ITM2B Antibody [NBP1-36963] - Paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary antibody at 10 ug/mL, 1 hr incubation followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/ITM2B-Antibody-Flow-Cytometry-NBP1-36963-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: ITM2B Antibody [NBP1-36963] - Paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary antibody at 10 ug/mL, 1 hr incubation followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi apparatus and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/ITM2B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-36963-img0007.jpg)
![Immunohistochemistry-Paraffin: CRIPTO Antibody [NBP1-68841] - Cripto1 Antibody [NBP1-68841] (3.8ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/CRIPTO-Antibody-Immunohistochemistry-Paraffin-NBP1-68841-img0002.jpg "Immunohistochemistry-Paraffin: CRIPTO Antibody [NBP1-68841] - Cripto1 Antibody [NBP1-68841] (3.8ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody [NBP3-03282] - Analysis of extracts of various cell lines, using Gastrin-releasing Peptide/Bombesin/Neuromedin C antibody (NBP3-03282) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Gastrin-releasing-Peptide-Bombesin-Neuromedin-C-Antibody-Western-Blot-NBP3-03282-img0004.jpg "Western Blot: Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody [NBP3-03282] - Analysis of extracts of various cell lines, using Gastrin-releasing Peptide/Bombesin/Neuromedin C antibody (NBP3-03282) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin: Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody [NBP3-03282] - Mouse heart using Gastrin-releasing Peptide/Bombesin/Neuromedin C Rabbit pAb (NBP3-03282) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Gastrin-releasing-Peptide-Bombesin-Neuromedin-C-Antibody-Immunohistochemistry-Paraffin-NBP3-03282-img0003.jpg)
![Western Blot: Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody [NBP3-03282] - Analysis of extracts of various cell lines, using Gastrin-releasing Peptide/Bombesin/Neuromedin C antibody (NBP3-03282) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Gastrin-releasing Peptide-Bombesin-Neuromedin C Antibody-Western Blot-NBP3-03282-img0005.jpg)
![Immunohistochemistry-Paraffin: Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody [NBP3-03282] - Rat brain using Gastrin-releasing Peptide/Bombesin/Neuromedin C Rabbit pAb (NBP3-03282) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Gastrin-releasing-Peptide-Bombesin-Neuromedin-C-Antibody-Immunohistochemistry-Paraffin-NBP3-03282-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody - BSA Free [Gastrin-releasing Peptide/Bombesin/Neuromedin C] - Immunofluorescence analysis of NIH/3T3 cells using Gastrin-releasing Peptide/Bombesin/Neuromedin C Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03282_rabbit-polyclonal-gastrin-releasing-peptide-bombesin-neuromedin-c-antibody-52202516345228.jpg)
![Immunocytochemistry/ Immunofluorescence: Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody - BSA Free [Gastrin-releasing Peptide/Bombesin/Neuromedin C] - Immunofluorescence analysis of PC-12 cells using Gastrin-releasing Peptide/Bombesin/Neuromedin C Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03282_rabbit-polyclonal-gastrin-releasing-peptide-bombesin-neuromedin-c-antibody-5220251639613.jpg)
![Western Blot: DSPP Antibody - Azide and BSA Free [NBP2-92546] - Western blot analysis of various lysates using DSPP Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp2-92546_rabbit-polyclonal-dspp-antibody-4220251430494.jpg%3Ft=1711070358 "Western Blot: DSPP Antibody - Azide and BSA Free [NBP2-92546] - Western blot analysis of various lysates using DSPP Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Western Blot: MTCO1 Antibody [NBP2-94458] - Analysis of extracts of various cell lines, using MTCO1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/MTCO1-Antibody-Western-Blot-NBP2-94458-img0012.jpg "Western Blot: MTCO1 Antibody [NBP2-94458] - Analysis of extracts of various cell lines, using MTCO1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry: MTCO1 Antibody - Azide and BSA Free [NBP2-94458] - Immunohistochemistry analysis of paraffin-embedded rat brain using MTCO1 Rabbit pAb (A17889) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp2-94458_rabbit-polyclonal-mtco1-antibody-52202519302978.jpg)
![Immunocytochemistry/ Immunofluorescence: MTCO1 Antibody - Azide and BSA Free [NBP2-94458] - Immunofluorescence analysis of PC-12 cells using MTCO1 Rabbit pAb (A17889) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94458_rabbit-polyclonal-mtco1-antibody-52202519313440.jpg)
![Immunohistochemistry: MTCO1 Antibody - Azide and BSA Free [NBP2-94458] - Immunohistochemistry analysis of paraffin-embedded rat kidney using MTCO1 Rabbit pAb (A17889) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp2-94458_rabbit-polyclonal-mtco1-antibody-52202519313412.jpg)
![Immunohistochemistry: MTCO1 Antibody - Azide and BSA Free [NBP2-94458] - Immunohistochemistry analysis of paraffin-embedded mouse heart using MTCO1 Rabbit pAb (A17889) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp2-94458_rabbit-polyclonal-mtco1-antibody-522025193134.jpg)
![Immunohistochemistry: MTCO1 Antibody - Azide and BSA Free [NBP2-94458] - Immunohistochemistry analysis of paraffin-embedded mouse kidney using MTCO1 Rabbit pAb (A17889) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp2-94458_rabbit-polyclonal-mtco1-antibody-52202519302999.jpg)