| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48295 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85353-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-51590SS | |||
| Azide and BSA Free | NBP2-80874 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-59632 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44978 | |||
| Azide and BSA Free | NBP2-34605-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44979 | |||
| Azide and BSA Free | NBP2-34434-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-34205-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-94150-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85352-25ul | |||
![Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody (NGFR5) - Azide and BSA Free [NBP2-47965] - Human Adrenal Gland stained with NGFR Mouse Monoclonal Antibody (NGFR5).](http://images.novusbio.com/fullsize/NGFR-TNFRSF16-p75NTR-Antibody-NGFR5-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47965-img0002.jpg "Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody (NGFR5) - Azide and BSA Free [NBP2-47965] - Human Adrenal Gland stained with NGFR Mouse Monoclonal Antibody (NGFR5).")
![Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody (NGFR5) - Azide and BSA Free [NBP2-47965] - Human Melanoma stained with NGFR Mouse Monoclonal Antibody (NGFR5).](http://images.novusbio.com/fullsize/NGFR-TNFRSF16-p75NTR-Antibody-NGFR5-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47965-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody [NBP1-33454] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33454-img0003.jpg "Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody [NBP1-33454] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-33454] - Rat cervix. 14-3-3 beta antibody [N1C2] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-33454-img0007.jpg)
![Western Blot: 14-3-3 beta/alpha Antibody [NBP1-33454] - Sample (20 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Western-Blot-NBP1-33454-img0005.jpg)
![Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-33454] - Paraffin-embedded SW480 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-33454-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 beta antibody [N1C2] (NBP1-33454) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33454_rabbit-polyclonal-14-3-3-beta-alpha-antibody-western-blot-2611202518274621.jpg)
![Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with 14-3-3 beta antibody [N1C2] (NBP1-33454) diluted at 1:10000 and competitor's antibody (sc-628) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33454_rabbit-polyclonal-14-3-3-beta-alpha-antibody-western-blot-2611202518431912.jpg)
![Western Blot: NSP 5 alpha 3 alpha Antibody [NBP1-85353] - Analysis in human cell line EFO-21.](http://images.novusbio.com/fullsize/NSP-5-alpha-3-alpha-Antibody-Western-Blot-NBP1-85353-img0009.jpg "Western Blot: NSP 5 alpha 3 alpha Antibody [NBP1-85353] - Analysis in human cell line EFO-21.")
![Immunohistochemistry-Paraffin: NSP 5 alpha 3 alpha Antibody [NBP1-85353] - Staining of human endometrium.](http://images.novusbio.com/fullsize/NSP-5-alpha-3-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-85353-img0015.jpg)
![Western Blot: NSP 5 alpha 3 alpha Antibody [NBP1-85353] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/NSP-5-alpha-3-alpha-Antibody-Western-Blot-NBP1-85353-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: NSP 5 alpha 3 alpha Antibody [NBP1-85353] - Staining of human colon, endometrium, liver and testis using Anti-SPECC1 antibody NBP1-85353 (A) shows similar protein distribution across tissues to independent antibody NBP1-85352 (B).](http://images.novusbio.com/fullsize/NSP-5-alpha-3-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-85353-img0010.jpg)
![Immunohistochemistry-Paraffin: NSP 5 alpha 3 alpha Antibody [NBP1-85353] - Staining of human testis.](http://images.novusbio.com/fullsize/NSP-5-alpha-3-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-85353-img0012.jpg)
![Immunohistochemistry-Paraffin: NSP 5 alpha 3 alpha Antibody [NBP1-85353] - Staining of human colon.](http://images.novusbio.com/fullsize/NSP-5-alpha-3-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-85353-img0013.jpg)
![Immunohistochemistry-Paraffin: NSP 5 alpha 3 alpha Antibody [NBP1-85353] - Staining of human liver.](http://images.novusbio.com/fullsize/NSP-5-alpha-3-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-85353-img0014.jpg)
![Western Blot: NGF R/TNFRSF16/p75NTR Antibody (2F1C2) [NBP1-51590] - Analysis using p75NTR/NGF Receptor mouse mAb against NGFR-hIgGFc transfected HEK293 cell lysate.](http://images.novusbio.com/fullsize/NGF-R-TNFRSF16-p75NTR-Antibody-2F1C2-Western-Blot-NBP1-51590-img0009.jpg "Western Blot: NGF R/TNFRSF16/p75NTR Antibody (2F1C2) [NBP1-51590] - Analysis using p75NTR/NGF Receptor mouse mAb against NGFR-hIgGFc transfected HEK293 cell lysate.")
![Immunocytochemistry/Immunofluorescence: NGF R/TNFRSF16/p75NTR Antibody (2F1C2) [NBP1-51590] - Analysis of EC cells using p75NTR/NGF Receptor mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/NGF-R-TNFRSF16-p75NTR-Antibody-2F1C2-Immunocytochemistry-Immunofluorescence-NBP1-51590-img0008.jpg)
![Flow Cytometry: NGF R/TNFRSF16/p75NTR Antibody (2F1C2) [NBP1-51590] - Analysis of EC cells using p75NTR/NGF Receptor mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/NGF-R-TNFRSF16-p75NTR-Antibody-2F1C2-Flow-Cytometry-NBP1-51590-img0007.jpg)
![Western Blot: p53 Antibody [NBP2-19667] - p53 antibody [N1], N-term detects p53 protein at nucleus by immunofluorescent analysis.Samples: HCT 116 cells mock (left) and treated with 30 uM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.Green: p53 protein stained by p53 antibody [N1], N-term.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/p53-Antibody-Western-Blot-NBP2-19667-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: p53 Antibody [NBP2-19667] - p53 antibody [N1], N-term detects p53 protein at nucleus by immunofluorescent analysis.Samples: HCT 116 cells mock (left) and treated with 30 uM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.Green: p53 protein stained by p53 antibody [N1], N-term.Blue: Hoechst 33342 staining.Scale bar = 10 um.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: p53 Antibody [NBP2-19667] - Confocal immunofluorescence analysis of paraformaldehyde-fixed U2OS, using p53 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/p53-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19667-img0003.jpg)
![<b>Biological Strategies Validation. </b>Immunoprecipitation: p53 Antibody [NBP2-19667] - Immunoprecipitation of p53 protein. HCT116 lysates with 30uM cisplatin treatment for 24 hours were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2. 5 ug of anti-p53 antibody. (A) Input, 20ug of HCT116 lysates. The precipitated protein was detected by NBP2-19667. anti-Rabbit IgG Kit was used in the Western blot.](http://images.novusbio.com/fullsize/p53-Antibody-Immunoprecipitation-NBP2-19667-img0007.jpg)
![Whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [N1], N-term (NBP2-19667) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19667_rabbit-polyclonal-p53-antibody-western-blot-2611202518354313.jpg)
![Western Blot: p53 Antibody (TP53/1799R) [NBP2-59631] - Western blot analysis of HeLa cell lysate using p53 antibody (TP53/1799R).](http://images.novusbio.com/fullsize/p53-Antibody-TP53-1799R-Western-Blot-NBP2-59631-img0005.jpg "Western Blot: p53 Antibody (TP53/1799R) [NBP2-59631] - Western blot analysis of HeLa cell lysate using p53 antibody (TP53/1799R).")
![Immunohistochemistry-Paraffin: p53 Antibody (TP53/1799R) [NBP2-59631] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained withp53 Recombinant Rabbit Monoclonal Antibody (TP53/1799R)](http://images.novusbio.com/fullsize/p53-Antibody-TP53-1799R-Immunohistochemistry-Paraffin-NBP2-59631-img0002.jpg)
![Flow Cytometry: p53 Antibody (TP53/1799R) [NBP2-59631] - Flow Cytometric Analysis of HeLa cells using p53 Recombinant Rabbit Monoclonal Antibody (TP53/1799R) followed by goat anti-rabbit IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/p53-Antibody-TP53-1799R-Flow-Cytometry-NBP2-59631-img0004.jpg)
![Western Blot: p53 Antibody (TP53/1799R) [NBP2-59631] - Western Blot Analysis of HeLa cell lysate using p53 Antibody (TP53/1799R).](http://images.novusbio.com/fullsize/p53-Antibody-TP53-1799R-Western-Blot-NBP2-59631-img0003.jpg)
![Protein Array: p53 Antibody (TP53/1799R) [NBP2-59631] - containing more than 19,000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary) produces when binding to a particular protein on the HuProt array. Z-scores are described in units of standard deviations above the mean value of all signals generated on that array. If targets on HuProt are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of an Ab to its intended target. An Ab is considered to specific to its intended target, if the Ab has an S-score of at least 2.5. For example, if Ab binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Ab to protein X is equal to 29](http://images.novusbio.com/fullsize/p53-Antibody-TP53-1799R-Protein-Array-NBP2-59631-img0001.jpg)
![Western Blot: p53 Antibody (DO-7) [NBP2-34308] - Western Blot Analysis of human HeLa cell lysate using p53 Mouse Monoclonal Antibody (DO-7).](http://images.novusbio.com/fullsize/p53-Antibody-DO-7-Western-Blot-NBP2-34308-img0004.jpg "Western Blot: p53 Antibody (DO-7) [NBP2-34308] - Western Blot Analysis of human HeLa cell lysate using p53 Mouse Monoclonal Antibody (DO-7).")
![Immunohistochemistry-Paraffin: p53 Antibody (DO-7) [NBP2-34308] - Formalin-fixed, paraffin-embedded human colon stained with p53 MAb (DO-7)](http://images.novusbio.com/fullsize/p53-Antibody-DO-7-Immunohistochemistry-Paraffin-NBP2-34308-img0001.jpg)
![Simple Western: p53 Antibody (DO-7) [NBP2-34308] - Simple Western lane view shows a specific band for p53 in 0.2 mg/ml of MDA-MB-231 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/p53-Antibody-DO-7-Simple-Western-NBP2-34308-img0002.jpg)
![Simple Western: p53 Antibody (DO-7) [NBP2-34308] - Electropherogram image of the corresponding Simple western lane view. p53 antibody was used at 10 ug/ml dilution on MDA-MB-231 lysate(s) respectively.](http://images.novusbio.com/fullsize/p53-Antibody-DO-7-Simple-Western-NBP2-34308-img0003.jpg)
![Western Blot: p53 Antibody (SPM590) [NBP2-34387] - Western Blot Analysis of human HeLa cell lysate using p53 Antibody (SPM590).](http://images.novusbio.com/fullsize/p53-Antibody-SPM590-Western-Blot-NBP2-34387-img0003.jpg "Western Blot: p53 Antibody (SPM590) [NBP2-34387] - Western Blot Analysis of human HeLa cell lysate using p53 Antibody (SPM590).")
![Immunohistochemistry-Paraffin: p53 Antibody (SPM590) [NBP2-34387] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with P53 Monoclonal antibody (SPM590)](http://images.novusbio.com/fullsize/p53-Antibody-SPM590-Immunohistochemistry-Paraffin-NBP2-34387-img0002.jpg)
![Immunohistochemistry-Paraffin: DRP1 Antibody [NBP2-34205] - Staining in human cerebral cortex and pancreas tissues.. Corresponding DNM1L RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/DRP1-Antibody-Immunohistochemistry-Paraffin-NBP2-34205-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: DRP1 Antibody [NBP2-34205] - Staining in human cerebral cortex and pancreas tissues.. Corresponding DNM1L RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: DRP1 Antibody [NBP2-34205] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/DRP1-Antibody-Western-Blot-NBP2-34205-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: DRP1 Antibody [NBP2-34205] - Staining of human cell line U-251 MG shows localization to cytosol and vesicles.](http://images.novusbio.com/fullsize/DRP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-34205-img0010.jpg)
![Immunohistochemistry-Paraffin: DRP1 Antibody [NBP2-34205] - Staining of human pancreas shows strong cytoplasmic positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/DRP1-Antibody-Immunohistochemistry-Paraffin-NBP2-34205-img0011.jpg)
![Immunohistochemistry-Paraffin: DRP1 Antibody [NBP2-34205] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/DRP1-Antibody-Immunohistochemistry-Paraffin-NBP2-34205-img0012.jpg)
![Immunohistochemistry-Paraffin: DRP1 Antibody [NBP2-34205] - Staining of human prostate shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/DRP1-Antibody-Immunohistochemistry-Paraffin-NBP2-34205-img0014.jpg)
![Western Blot: CLN5 Antibody [NBP1-94150] - Analysis in control (vector only transfected HEK293T lysate) and CLN5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/CLN5-Antibody-Western-Blot-NBP1-94150-img0009.jpg "Western Blot: CLN5 Antibody [NBP1-94150] - Analysis in control (vector only transfected HEK293T lysate) and CLN5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: CLN5 Antibody [NBP1-94150] - Staining of human gallbladder shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CLN5-Antibody-Immunohistochemistry-Paraffin-NBP1-94150-img0008.jpg)
![Western Blot: NSP 5 alpha 3 alpha Antibody [NBP1-85352] - Analysis using Anti-SPECC1 antibody NBP1-85352 (A) shows similar pattern to independent antibody NBP1-85353 (B).](http://images.novusbio.com/fullsize/NSP-5-alpha-3-alpha-Antibody-Western-Blot-NBP1-85352-img0006.jpg "<b>Independent Antibodies Validation. </b>Western Blot: NSP 5 alpha 3 alpha Antibody [NBP1-85352] - Analysis using Anti-SPECC1 antibody NBP1-85352 (A) shows similar pattern to independent antibody NBP1-85353 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: NSP 5 alpha 3 alpha Antibody [NBP1-85352] - Staining of human liver.](http://images.novusbio.com/fullsize/NSP-5-alpha-3-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-85352-img0009.jpg)
![Immunohistochemistry-Paraffin: NSP 5 alpha 3 alpha Antibody [NBP1-85352] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferus ducts.](http://images.novusbio.com/fullsize/NSP-5-alpha-3-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-85352-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: NSP 5 alpha 3 alpha Antibody [NBP1-85352] - Staining of human colon, endometrium, liver and testis using Anti-SPECC1 antibody NBP1-85352 (A) shows similar protein distribution across tissues to independent antibody NBP1-85353 (B).](http://images.novusbio.com/fullsize/NSP-5-alpha-3-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-85352-img0005.jpg)
![Immunohistochemistry-Paraffin: NSP 5 alpha 3 alpha Antibody [NBP1-85352] - Staining of human colon.](http://images.novusbio.com/fullsize/NSP-5-alpha-3-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-85352-img0007.jpg)
![Immunohistochemistry-Paraffin: NSP 5 alpha 3 alpha Antibody [NBP1-85352] - Staining of human endometrium.](http://images.novusbio.com/fullsize/NSP-5-alpha-3-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-85352-img0008.jpg)
![Immunohistochemistry-Paraffin: NSP 5 alpha 3 alpha Antibody [NBP1-85352] - Staining of human testis.](http://images.novusbio.com/fullsize/NSP-5-alpha-3-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-85352-img0010.jpg)
![Western Blot: RelA/NFkB p65 Antibody (PSH0-27) [NBP3-32648] - Western blot analysis of RelA/NFkB p65 on different lysates with Rabbit anti-RelA/NFkB p65 antibody (NBP3-32648) at 1/500 dilution. Lane 1: Hela cell lysate (control) Lane 2: Hela-RELA-KO (1) Lane 3: Hela-RELA-KO (2) Lysates/proteins at 35 ug/Lane. Predicted band size: 60 kDa Observed band size: 65 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32648) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32648_rabbit-rela-nfkb-p65-mab-psh0-27-278202415212453.jpg "Western Blot: RelA/NFkB p65 Antibody (PSH0-27) [NBP3-32648] - Western blot analysis of RelA/NFkB p65 on different lysates with Rabbit anti-RelA/NFkB p65 antibody (NBP3-32648) at 1/500 dilution. Lane 1: Hela cell lysate (control) Lane 2: Hela-RELA-KO (1) Lane 3: Hela-RELA-KO (2) Lysates/proteins at 35 ug/Lane. Predicted band size: 60 kDa Observed band size: 65 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32648) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: RelA/NFkB p65 Antibody (PSH0-27) [NBP3-32648] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-RelA/NFkB p65 antibody (NBP3-32648) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32648) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32648_rabbit-rela-nfkb-p65-mab-psh0-27-29820241435276.jpg)
![Immunocytochemistry/ Immunofluorescence: RelA/NFkB p65 Antibody (PSH0-27) [NBP3-32648] - Immunocytochemistry analysis of NIH/3T3 cells treated with or without 50μg/mL TNF-alpha for 20 minutes labeling RelA/NFkB p65 with Rabbit anti-RelA/NFkB p65 antibody (NBP3-32648) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RelA/NFkB p65 antibody (NBP3-32648) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32648_rabbit-rela-nfkb-p65-mab-psh0-27-29202410484811.jpg)
![Flow Cytometry: RelA/NFkB p65 Antibody (PSH0-27) [NBP3-32648] - Flow cytometric analysis of NIH/3T3 cells labeling RelA/NFkB p65. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32648, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32648_rabbit-rela-nfkb-p65-mab-psh0-27-2920241637214.jpg)