| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43954 | |||
| BSA Free | NBP2-34495-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71556 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-126SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90125-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70715 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25148SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86169-25ul | |||
![Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining in human skin and skeletal muscle tissues. Corresponding p53 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunohistochemistry-Paraffin-NBP2-34495-img0021.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining in human skin and skeletal muscle tissues. Corresponding p53 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: p53 Antibody (CL2199) [NBP2-34495] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-P53 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-PPIB monoclonal antibody was used as loading control.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Western-Blot-NBP2-34495-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495] - Staining in U2OS cell line with Anti-p53 monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunocytochemistry-Immunofluorescence-NBP2-34495-img0014.jpg)
![Western Blot: p53 Antibody (CL2199) [NBP2-34495] - Lane 1: Marker [kDa] Lane 2: Human cell line U-251](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Western-Blot-NBP2-34495-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495] - Staining of human cell line A431 showing cell cycle dependent nuclear (without nucleoli)Staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunocytochemistry-Immunofluorescence-NBP2-34495-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495] - Staining in MCF7 cell line with Anti-p53 monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunocytochemistry-Immunofluorescence-NBP2-34495-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495] - Staining in U251 cell line with Anti-p53 monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunocytochemistry-Immunofluorescence-NBP2-34495-img0013.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining of human colorectal cancer shows strong nuclear positivity in tumor cells, but not in normal mucosa.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunohistochemistry-Paraffin-NBP2-34495-img0017.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining of human lung cancer (squamous cell carcinoma) shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunohistochemistry-Paraffin-NBP2-34495-img0018.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining of human skin shows moderate to strong nuclear positivity in a subset of epidermal cells.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunohistochemistry-Paraffin-NBP2-34495-img0019.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunohistochemistry-Paraffin-NBP2-34495-img0020.jpg)
![Simple Western: p53 Antibody (CL2199) [NBP2-34495] - Simple Western lane view shows a specific band for P53 in 0.2 mg/ml of Hek293T lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Simple-Western-NBP2-34495-img0009.jpg)
![Simple Western: p53 Antibody (CL2199) [NBP2-34495] - Electropherogram image(s) of corresponding Simple Western lane view. p53 antibody was used at 1:50 dilution on Hek293T lysate(s).](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Simple-Western-NBP2-34495-img0011.jpg)
![Western Blot: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Gephyrin monoclonal antibody.](http://images.novusbio.com/fullsize/Gephyrin-GPHN-Antibody-3B6-Western-Blot-NBP2-03449-img0018.jpg "Western Blot: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Gephyrin monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Gephyrin.](http://images.novusbio.com/fullsize/Gephyrin-GPHN-Antibody-3B6-Immunocytochemistry-Immunofluorescence-NBP2-03449-img0012.jpg)
![Immunohistochemistry-Paraffin: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - Staining of paraffin-embedded Human prostate tissue using anti-Gephyrin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Gephyrin-GPHN-Antibody-3B6-Immunohistochemistry-Paraffin-NBP2-03449-img0016.jpg)
![Flow Cytometry: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Gephyrin antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Gephyrin-GPHN-Antibody-3B6-Flow-Cytometry-NBP2-03449-img0011.jpg)
![Western Blot: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Gephyrin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Gephyrin.](http://images.novusbio.com/fullsize/Gephyrin-GPHN-Antibody-3B6-Western-Blot-NBP2-03449-img0017.jpg)
![Immunohistochemistry-Paraffin: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Gephyrin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Gephyrin-GPHN-Antibody-3B6-Immunohistochemistry-Paraffin-NBP2-03449-img0013.jpg)
![Immunohistochemistry-Paraffin: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Gephyrin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Gephyrin-GPHN-Antibody-3B6-Immunohistochemistry-Paraffin-NBP2-03449-img0014.jpg)
![Immunohistochemistry-Paraffin: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - Staining of paraffin-embedded Human liver tissue using anti-Gephyrin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Gephyrin-GPHN-Antibody-3B6-Immunohistochemistry-Paraffin-NBP2-03449-img0015.jpg)
![Flow Cytometry: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - Analysis of Jurkat cells, using anti-Gephyrin antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Gephyrin-GPHN-Antibody-3B6-Flow-Cytometry-NBP2-03449-img0010.jpg)
![Western Blot: Gephyrin/GPHN Antibody [NBP2-16641] - Human tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gephyrin antibody [N2C1], Internal diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Gephyrin-GPHN-Antibody-Western-Blot-NBP2-16641-img0013.jpg "Western Blot: Gephyrin/GPHN Antibody [NBP2-16641] - Human tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gephyrin antibody [N2C1], Internal diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: Gephyrin/GPHN Antibody [NBP2-16641] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Gephyrin-Antibody-Western-Blot-NBP2-16641-img0002.jpg)
![Immunoprecipitation: Gephyrin/GPHN Antibody [NBP2-16641] - A431 whole cell extracts using 5 ug of Gephyrin antibody [N2C1], Internal. Western blot analysis was performed using Gephyrin antibody [N2C1], Internal. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Gephyrin-GPHN-Antibody-Immunoprecipitation-NBP2-16641-img0006.jpg)
![Gephyrin antibody [N2C1], Internal detects Gephyrin protein expression by immunohistochemical analysis.Sample: Frozen sectioned E13.5 Rat brain. Green: Gephyrin protein stained by Gephyrin antibody [N2C1], Internal (NBP2-16641) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-16641_rabbit-polyclonal-gephyrin-gphn-antibody-immunohistochemistry-frozen-2611202518443520.jpg)
![Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gephyrin antibody [N2C1], Internal (NBP2-16641) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16641_rabbit-polyclonal-gephyrin-gphn-antibody-western-blot-261120251962711.jpg)
![Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gephyrin antibody [N2C1], Internal (NBP2-16641) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16641_rabbit-polyclonal-gephyrin-gphn-antibody-western-blot-261120251843195.jpg)
![Gephyrin antibody [N2C1], Internal detects Gephyrin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.Gephyrin stained by Gephyrin antibody [N2C1], Internal (NBP2-16641) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-16641_rabbit-polyclonal-gephyrin-gphn-antibody-immunohistochemistry-paraffin-2611202518202312.jpg)
![Gephyrin antibody [N2C1], Internal detects Gephyrin protein by immunofluorescent analysis.Sample: DIV10 rat E18 primary hippocampal neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: Gephyrin protein stained by Gephyrin antibody [N2C1], Internal (NBP2-16641) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-16641_rabbit-polyclonal-gephyrin-gphn-antibody-immunocytochemistry-immunofluorescence-2611202518274620.jpg)
![Western Blot: CLOCK Antibody [NB100-126] - Lanes 3-5 contained alpha CLOCK immunoprecipitated mouse DNA (15, 10 and 5 ul of antisera added respectively to the 900 ul of sonicated chromatin sample in the ChIP assay.](http://images.novusbio.com/fullsize/CLOCK-Antibody-Western-Blot-NB100-126-img0002.jpg "Western Blot: CLOCK Antibody [NB100-126] - Lanes 3-5 contained alpha CLOCK immunoprecipitated mouse DNA (15, 10 and 5 ul of antisera added respectively to the 900 ul of sonicated chromatin sample in the ChIP assay.")
![Western Blot: Brg1 Antibody (GT2712) [NBP3-13537] - Wild-type (WT) and Brg1 knockout (KO) 293T cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Brg1 antibody [GT2712] (NBP3-13537) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Brg1-Antibody-GT2712-Western-Blot-NBP3-13537-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Brg1 Antibody (GT2712) [NBP3-13537] - Wild-type (WT) and Brg1 knockout (KO) 293T cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Brg1 antibody [GT2712] (NBP3-13537) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Brg1 Antibody (GT2712) [NBP3-13537] - Brg1 antibody [GT2712] detects Brg1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Brg1 protein stained by Brg1 antibody [GT2712] (NBP3-13537) diluted at 1:100. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Brg1-Antibody-GT2712-Immunocytochemistry-Immunofluorescence-NBP3-13537-img0004.jpg)
![Immunohistochemistry-Frozen: Brg1 Antibody (GT2712) [NBP3-13537] - Brg1 antibody [GT2712] detects Brg1 protein by immunohistochemical analysis. Sample: Frozen-sectioned rat E13.5 brain. Green: Brg1 stained by Brg1 antibody [GT2712] (NBP3-13537) diluted at 1:250. Red: SOX2, stained by SOX2 antibody [N1C3] (NBP1-33766) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Brg1-Antibody-GT2712-Immunohistochemistry-Frozen-NBP3-13537-img0007.jpg)
![Western Blot: Brg1 Antibody (GT2712) [NBP3-13537] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Brg1 antibody [GT2712] (NBP3-13537) diluted at 1:1000.](http://images.novusbio.com/fullsize/Brg1-Antibody-GT2712-Western-Blot-NBP3-13537-img0001.jpg)
![Western Blot: Brg1 Antibody (GT2712) [NBP3-13537] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Brg1 antibody [GT2712] (NBP3-13537) diluted at 1:1000.](http://images.novusbio.com/fullsize/Brg1-Antibody-GT2712-Western-Blot-NBP3-13537-img0005.jpg)
![Western Blot: Brg1 Antibody (GT2712) [NBP3-13537] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Brg1 antibody [GT2712] (NBP3-13537) diluted at 1:1000.](http://images.novusbio.com/fullsize/Brg1-Antibody-GT2712-Western-Blot-NBP3-13537-img0006.jpg)
![Immunohistochemistry-Paraffin: Brg1 Antibody (GT2712) [NBP3-13537] - Brg1 antibody [GT2712] detects Brg1 protein at nucleus in human oral carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human oral carcinoma. Brg1 antibody [GT2712] (NBP3-13537) diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Brg1-Antibody-GT2712-Immunohistochemistry-Paraffin-NBP3-13537-img0002.jpg)
![Immunohistochemistry-Paraffin: Brg1 Antibody (GT2712) [NBP3-13537] - Brg1 antibody [GT2712] detects Brg1 protein at nucleus in human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. Brg1 antibody [GT2712] (NBP3-13537) diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Brg1-Antibody-GT2712-Immunohistochemistry-Paraffin-NBP3-13537-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Caspase-3 Antibody [NBP1-90125] - Staining of human cell line U-251 MG shows localization to nucleoplasm and mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90125-img0015.jpg "Immunocytochemistry/Immunofluorescence: Caspase-3 Antibody [NBP1-90125] - Staining of human cell line U-251 MG shows localization to nucleoplasm and mitochondria. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free [NBP3-25558] - MC1 Receptor antibody [HL1471] detects MC1 Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. MC1 Receptor stained by MC1 Receptor antibody [HL1471] (NBP3-25558) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-2120249432833.jpg "Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free [NBP3-25558] - MC1 Receptor antibody [HL1471] detects MC1 Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. MC1 Receptor stained by MC1 Receptor antibody [HL1471] (NBP3-25558) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free [NBP3-25558] - MC1 Receptor antibody [HL1471] detects MC1 Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. MC1 Receptor stained by MC1 Receptor antibody [HL1471] (NBP3-25558) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-2120249415999.jpg)
![Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free [NBP3-25558] - MC1 Receptor antibody [HL1471] detects MC1 Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. MC1 Receptor stained by MC1 Receptor antibody [HL1471] (NBP3-25558) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-212024943553.jpg)
![Western Blot: Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free [NBP3-25558] - Various extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MC1 Receptor antibody [HL1471] (NBP3-25558) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-2120249374992.jpg)
![Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free [NBP3-25558] - MC1 Receptor antibody [HL1471] detects MC1 Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. MC1 Receptor stained by MC1 Receptor antibody [HL1471] (NBP3-25558) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-2120249374949.jpg)
![Western Blot: Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free [NBP3-25558] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MC1 Receptor antibody [HL1471] (NBP3-25558) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-2120249432886.jpg)
![Immunocytochemistry/Immunofluorescence: Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free [NBP3-25558] - MC1 Receptor antibody [HL1471] detects MC1 Receptor protein at cytoplasm by immunofluorescent analysis. Sample: A375 cells were fixed in ice-cold MeOH for 5 min. Green: MC1 Receptor stained by MC1 Receptor antibody [HL1471] (NBP3-25558) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-2120249342758.jpg)
![Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free [NBP3-25558] - MC1 Receptor antibody [HL1471] detects MC1 Receptor protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded A375 xenograft . MC1 Receptor stained by MC1 Receptor antibody [HL1471] (NBP3-25558) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-2120249362712.jpg)
![Frozen-sectioned mouse cerebellum.Green: MC1 Receptor stained by MC1 Receptor antibody [HL1471] diluted at 1:100.Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-immunohistochemistry-frozen-119202513498.png)
![<b>Independent Antibodies Validation. </b>Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membranes were blotted with Melanocortin-1 R/MC1R antibody [HL1471] (NBP3-25558) diluted at 1:50000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated withand does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-western-blot-141120251843244.jpg)
![<b>Independent Antibodies Validation. </b>Various tissue extracts (50 ug) was separated by 12% SDS-PAGE, and the membranes were blotted with Melanocortin-1 R/MC1R antibody [HL1471] (NBP3-25558) diluted at 1:1000 and competitor's antibody (Competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated withand does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-western-blot-1411202518433316.jpg)
![<b>Independent Antibodies Validation, Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Non-transfected (-) and transfected (+) unboiled A375 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with Melanocortin-1 R/MC1R antibody [HL1471] (NBP3-25558) diluted at 1:5000 and competitor's antibody (Highly Cited Competitor) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated withand does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-western-blot-14112025183376.jpg)
![Western Blot: FDFT1 Antibody (2F10) [NBP2-01170] Analysis of extracts (35ug) from 9 different cell lines by using anti-FDFT1 monoclonal antibody.](http://images.novusbio.com/fullsize/FDFT1-Antibody-2F10-Western-Blot-NBP2-01170-img0011.jpg "Western Blot: FDFT1 Antibody (2F10) [NBP2-01170] Analysis of extracts (35ug) from 9 different cell lines by using anti-FDFT1 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: FDFT1 Antibody (2F10) [NBP2-01170] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY FDFT1.](http://images.novusbio.com/fullsize/FDFT1-Antibody-2F10-Immunocytochemistry-Immunofluorescence-NBP2-01170-img0010.jpg)
![Immunohistochemistry-Paraffin: FDFT1 Antibody (2F10) [NBP2-01170] - Staining of paraffin-embedded Human prostate tissue using anti-FDFT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FDFT1-Antibody-2F10-Immunohistochemistry-Paraffin-NBP2-01170-img0009.jpg)
![Western Blot: FDFT1 Antibody (2F10) [NBP2-01170] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FDFT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FDFT1.](http://images.novusbio.com/fullsize/FDFT1-Antibody-2F10-Western-Blot-NBP2-01170-img0001.jpg)
![Immunohistochemistry-Paraffin: FDFT1 Antibody (2F10) [NBP2-01170] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-FDFT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FDFT1-Antibody-2F10-Immunohistochemistry-Paraffin-NBP2-01170-img0002.jpg)
![Immunohistochemistry-Paraffin: FDFT1 Antibody (2F10) [NBP2-01170] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-FDFT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FDFT1-Antibody-2F10-Immunohistochemistry-Paraffin-NBP2-01170-img0003.jpg)
![Immunohistochemistry-Paraffin: FDFT1 Antibody (2F10) [NBP2-01170] - Staining of paraffin-embedded Human breast tissue using anti-FDFT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FDFT1-Antibody-2F10-Immunohistochemistry-Paraffin-NBP2-01170-img0004.jpg)
![Immunohistochemistry-Paraffin: FDFT1 Antibody (2F10) [NBP2-01170] - Staining of paraffin-embedded Human endometrium tissue using anti-FDFT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FDFT1-Antibody-2F10-Immunohistochemistry-Paraffin-NBP2-01170-img0005.jpg)
![Immunohistochemistry-Paraffin: FDFT1 Antibody (2F10) [NBP2-01170] - Staining of paraffin-embedded Human Kidney tissue using anti-FDFT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FDFT1-Antibody-2F10-Immunohistochemistry-Paraffin-NBP2-01170-img0006.jpg)
![Immunohistochemistry-Paraffin: FDFT1 Antibody (2F10) [NBP2-01170] - Staining of paraffin-embedded Human liver tissue using anti-FDFT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FDFT1-Antibody-2F10-Immunohistochemistry-Paraffin-NBP2-01170-img0007.jpg)
![Immunohistochemistry-Paraffin: FDFT1 Antibody (2F10) [NBP2-01170] - Staining of paraffin-embedded Human lymphoma tissue using anti-FDFT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FDFT1-Antibody-2F10-Immunohistochemistry-Paraffin-NBP2-01170-img0008.jpg)
![Western Blot: HMGB1/HMG-1 Antibody (1C4T5) [HMGB1/HMG-1] - Western blot analysis of lysates from wild type (WT) and HMGB1/HMG-1 knockout (KO) HeLa cells using [KO Validated] HMGB1/HMG-1 Rabbit mAb at 1:6000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 0.5s.](http://images.novusbio.com/fullsize/nbp3-15755_rabbit-hmgb1-hmg-1-mab-1c4t5-52202517352784.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HMGB1/HMG-1 Antibody (1C4T5) [HMGB1/HMG-1] - Western blot analysis of lysates from wild type (WT) and HMGB1/HMG-1 knockout (KO) HeLa cells using [KO Validated] HMGB1/HMG-1 Rabbit mAb at 1:6000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 0.5s.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 Antibody (1C4T5) [NBP3-15755] - Confocal imaging of NIH-3T3 cells using [KO Validated] HMGB1 Rabbit mAb (dilution 1:100) (Red). The cells were counterstained with alpha-Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15755_rabbit-hmgb1-hmg-1-mab-1c4t5-immunocytochemistry-immunofluorescence-1962023104824.jpg)
![Immunohistochemistry: HMGB1/HMG-1 Antibody (1C4T5) [HMGB1/HMG-1] - Immunohistochemistry analysis of paraffin-embedded Rat liver using [KO Validated] HMGB1/HMG-1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15755_rabbit-hmgb1-hmg-1-mab-1c4t5-52202517344263.jpg)
![Immunohistochemistry: HMGB1/HMG-1 Antibody (1C4T5) [HMGB1/HMG-1] - Immunohistochemistry analysis of paraffin-embedded Mouse testis using [KO Validated] HMGB1/HMG-1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15755_rabbit-hmgb1-hmg-1-mab-1c4t5-52202517311012.jpg)
![Immunohistochemistry: HMGB1/HMG-1 Antibody (1C4T5) [HMGB1/HMG-1] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using [KO Validated] HMGB1/HMG-1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15755_rabbit-hmgb1-hmg-1-mab-1c4t5-52202517304984.jpg)
![Immunohistochemistry: HMGB1/HMG-1 Antibody (1C4T5) [HMGB1/HMG-1] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using [KO Validated] HMGB1/HMG-1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15755_rabbit-hmgb1-hmg-1-mab-1c4t5-52202517311085.jpg)
![Immunohistochemistry: HMGB1/HMG-1 Antibody (1C4T5) [HMGB1/HMG-1] - Immunohistochemistry analysis of paraffin-embedded Mouse colon using [KO Validated] HMGB1/HMG-1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15755_rabbit-hmgb1-hmg-1-mab-1c4t5-5220251734575.jpg)
![Immunohistochemistry: HMGB1/HMG-1 Antibody (1C4T5) [HMGB1/HMG-1] - Immunohistochemistry analysis of paraffin-embedded Mouse liver using [KO Validated] HMGB1/HMG-1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15755_rabbit-hmgb1-hmg-1-mab-1c4t5-52202517352740.jpg)
![Western Blot: HMGB1/HMG-1 Antibody (1C4T5) [NBP3-15755] - Western blot analysis of various lysates using [KO Validated] HMGB1/HMG-1 Rabbit mAb at 1:6000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 0.5s.](http://images.novusbio.com/fullsize/nbp3-15755_rabbit-hmgb1-hmg-1-mab-1c4t5-8220252010416.jpg)
![Western Blot: RPSA Antibody [NBP1-33002] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RPSA antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/RPSA-Antibody-Western-Blot-NBP1-33002-img0018.jpg "Western Blot: RPSA Antibody [NBP1-33002] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RPSA antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: RPSA Antibody [NBP1-33002] - CA922 xenograft, using RPSA antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/RPSA-Antibody-Immunohistochemistry-Paraffin-NBP1-33002-img0012.jpg)
![Western Blot: RPSA Antibody [NBP1-33002] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/RPSA-Antibody-Western-Blot-NBP1-33002-img0013.jpg)
![Western Blot: RPSA Antibody [NBP1-33002] - Whole cell extracts (30 ug) was separated by 10 % SDS-PAGE, and blotted with RPSA antibody diluted by 1:1000](http://images.novusbio.com/fullsize/RPSA-Antibody-Western-Blot-NBP1-33002-img0014.jpg)
![Western Blot: RPSA Antibody [NBP1-33002] - Liver protein expression in different experimental groups of Laminin R, AdipoR2 and SIRT1.Western Blotting analysis of protein expression in the liver on different experimental groups of aminin Receptor. Image shows demonstrative bands of the analyzed proteins and respective housekeeping protein (beta-tubulin) in liver. Data are expressed in mean +/- s.e.m. *p<0.05 Control diet and EGCG (CE) group versus Control diet and Water (CW) group. #p<0.05 High-fat diet and EGCG (HFE) group versus HFW group. $p<0.05 HFW versus CW. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0141227), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RPSA-Antibody-Western-Blot-NBP1-33002-img0015.jpg)
![Western Blot: RPSA Antibody [NBP1-33002] - Sample (30 ug of whole cell lysate). A: Jurkat. 12% SDS PAGE. RPSA Antibody diluted at 1:1000](http://images.novusbio.com/fullsize/RPSA-Antibody-Western-Blot-NBP1-33002-img0016.jpg)
![Western Blot: RPSA Antibody [NBP1-33002] - RPSA antibody detects RPSA protein by Western blot analysis. Mouse tissue extracts (50 ug) was separated by 10 % SDS-PAGE, and the membrane was blotted with RPSA antibody diluted by 1:1000.](http://images.novusbio.com/fullsize/RPSA-Antibody-Western-Blot-NBP1-33002-img0017.jpg)
![Immunohistochemistry-Paraffin: RPSA Antibody [NBP1-33002] - Mouse small intestine. RPSA antibody [N1C3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/RPSA-Antibody-Immunohistochemistry-Paraffin-NBP1-33002-img0009.jpg)
![Western Blot: RPSA Antibody [NBP1-33002] - RPSA antibody detects RPSA protein by Western blot analysis. Whole cell extracts (30 ug) was separated by 10 % SDS-PAGE, and blotted with RPSA antibody (NBP1-33002) diluted by 1:1000](http://images.novusbio.com/fullsize/nbp1-33002_rabbit-polyclonal-rpsa-antibody-9102024205675.jpg)
![Western Blot: HMGB1/HMG-1 Antibody (1F3) [NBP2-25148] - Analysis of lysates from different cell lines probed with mouse mAb to high mobility group protein B1, (HMGB1), NBP2-25148, dilution 1:2,000: [1] protein standard, [2] NIH-3T3, [3] C6, [4] HEK293, [5] HeLa, and [6] SH-SY5Y. The 25kDa band revealed by MCA-1F3 antibody, corresponds to HMGB1 protein.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-1F3-Western-Blot-NBP2-25148-img0009.jpg "Western Blot: HMGB1/HMG-1 Antibody (1F3) [NBP2-25148] - Analysis of lysates from different cell lines probed with mouse mAb to high mobility group protein B1, (HMGB1), NBP2-25148, dilution 1:2,000: [1] protein standard, [2] NIH-3T3, [3] C6, [4] HEK293, [5] HeLa, and [6] SH-SY5Y. The 25kDa band revealed by MCA-1F3 antibody, corresponds to HMGB1 protein.")
![Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 Antibody (1F3) [NBP2-25148] - Analysis of of HeLa cells stained with mouse mAb to HMGB1,NBP2-25148, dilution 1:2,000 in red and costained with rabbit pAb to GAPDH, dilution 1:2,000 in green. MCA-1F3 antibody stains the chromatin binding protein HMGB1, which is localized in the nuclei.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-1F3-Immunocytochemistry-Immunofluorescence-NBP2-25148-img0010.jpg)
![Flow Cytometry: HMGB1/HMG-1 Antibody (1F3) [NBP2-25148] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using HMGB1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-1F3-Flow-Cytometry-NBP2-25148-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 Antibody (1F3) [NBP2-25148] - HeLa cells grown in tissue culture and stained with NBP2-25148 (red), chicken polyclonal antibody to Vimentin NB300-223 (green) and DNA (blue). NBP2-25148 reveals strong nuclear staining which overlaps with the DNA stain.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-1F3-Immunocytochemistry-Immunofluorescence-NBP2-25148-img0008.jpg)
![Simple Western: HMGB1/HMG-1 Antibody (1F3) [NBP2-25148] - Simple Western lane view shows a specific band for HMGB1 in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-1F3-Simple-Western-NBP2-25148-img0005.jpg)
![Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody [NBP1-86169] - Staining of human skeletal muscle shows moderate granular cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Immunohistochemistry-Paraffin-NBP1-86169-img0010.jpg "Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody [NBP1-86169] - Staining of human skeletal muscle shows moderate granular cytoplasmic positivity in myocytes.")
![Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody [NBP1-86169] - Staining of human colon shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Immunohistochemistry-Paraffin-NBP1-86169-img0007.jpg)
![Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody [NBP1-86169] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Immunohistochemistry-Paraffin-NBP1-86169-img0009.jpg)
