| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38604-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95096-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03731-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59330-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59325-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61842-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12899-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59378-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61121-0.025ml | |||
![Western Blot: Nrf1 Antibody (4Q4E7) [NBP3-16161] - Western blot analysis of extracts of various cell lines, using Nrf1 Rabbit mAb (NBP3-16161) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/Nrf1-Antibody-4Q4E7-Western-Blot-NBP3-16161-img0003.jpg "Western Blot: Nrf1 Antibody (4Q4E7) [NBP3-16161] - Western blot analysis of extracts of various cell lines, using Nrf1 Rabbit mAb (NBP3-16161) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunohistochemistry-Paraffin: Nrf1 Antibody (4Q4E7) [NBP3-16161] - Immunohistochemistry of paraffin-embedded human colon using Nrf1 Rabbit mAb (NBP3-16161) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Nrf1-Antibody-4Q4E7-Immunohistochemistry-Paraffin-NBP3-16161-img0001.jpg)
![Western Blot: Nrf1 Antibody (4Q4E7) [NBP3-16161] - Western blot analysis of extracts of various cell lines, using Nrf1 Rabbit mAb (NBP3-16161) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Nrf1-Antibody-4Q4E7-Western-Blot-NBP3-16161-img0002.jpg)
![Chromatin Immunoprecipitation: Nrf1 Antibody (4Q4E7) [NBP3-16161] - Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using Nrf1 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-16161_rabbit-nrf1-mab-4q4e7-52202511383725.jpg)
![Immunohistochemistry: Coronin-1a Antibody [NBP3-38604] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen using Coronin-1a Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38604_rabbit-coronin-1a-pab-912202414201354.jpg "Immunohistochemistry: Coronin-1a Antibody [NBP3-38604] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen using Coronin-1a Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Immunohistochemistry: Coronin-1a Antibody [NBP3-38604] - Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using Coronin-1a Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38604_rabbit-coronin-1a-pab-912202414201323.jpg)
![Western Blot: Coronin-1a Antibody [NBP3-38604] - Western blot analysis of various lysates using Coronin-1a Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-38604_rabbit-coronin-1a-pab-912202415152849.jpg)
![Immunohistochemistry: Coronin-1a Antibody [NBP3-38604] - Immunohistochemistry analysis of paraffin-embedded Mouse liver using Coronin-1a Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38604_rabbit-coronin-1a-pab-912202414123825.jpg)
![Immunohistochemistry: Coronin-1a Antibody [NBP3-38604] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using Coronin-1a Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38604_rabbit-coronin-1a-pab-912202415152850.jpg)
![Immunohistochemistry: Coronin-1a Antibody [NBP3-38604] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using Coronin-1a Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38604_rabbit-coronin-1a-pab-91220241412386.jpg)
![Western Blot: RPE65 Antibody [NBP2-95096] - Analysis of extracts of various cell lines, using RPE65 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5s.](http://images.novusbio.com/fullsize/RPE65-Antibody-Western-Blot-NBP2-95096-img0003.jpg "Western Blot: RPE65 Antibody [NBP2-95096] - Analysis of extracts of various cell lines, using RPE65 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5s.")
![Immunohistochemistry: RPE65 Antibody [NBP2-95096] - Analysis of Mouse eye cells using RPE65 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RPE65-Antibody-Immunohistochemistry-NBP2-95096-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: RPE65 Antibody - BSA Free [NBP2-95096] - Perform microwave antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded mouse retina using RPE65 Rabbit pAb (A9841) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95096_rabbit-polyclonal-rpe65-antibody-52202519302965.jpg)
![Immunocytochemistry/ Immunofluorescence: RPE65 Antibody - BSA Free [NBP2-95096] - Perform microwave antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded rat retina using RPE65 Rabbit pAb (A9841) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95096_rabbit-polyclonal-rpe65-antibody-5220251931373.jpg)
![Western Blot: RPE65 Antibody - BSA Free [NBP2-95096] - Western blot analysis of lysates from Mouse eye, using RPE65 Rabbit pAb (A9841) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp2-95096_rabbit-polyclonal-rpe65-antibody-52202519294553.jpg)
![Western Blot: GRK2 Antibody [NBP3-03731] - Western blot analysis of extracts from normal (control) and GRK2 knockout (KO) 293T cells, using GRK2 antibody (NBP3-03731) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/GRK2-Antibody-Western-Blot-NBP3-03731-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: GRK2 Antibody [NBP3-03731] - Western blot analysis of extracts from normal (control) and GRK2 knockout (KO) 293T cells, using GRK2 antibody (NBP3-03731) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: GRK2 Antibody [NBP3-03731] - Paraffin-embedded mouse kidney using GRK2 antibody at dilution of 1:200 (40x lens).](http://images.novusbio.com/fullsize/GRK2-Antibody-Immunohistochemistry-Paraffin-NBP3-03731-img0003.jpg)
![Immunohistochemistry-Paraffin: GRK2 Antibody [NBP3-03731] - Paraffin-embedded rat brain using GRK2 antibody at dilution of 1:200 (40x lens).](http://images.novusbio.com/fullsize/GRK2-Antibody-Immunohistochemistry-Paraffin-NBP3-03731-img0001.jpg)
![Immunohistochemistry-Paraffin: GRK2 Antibody [NBP3-03731] - Paraffin-embedded human esophageal cancer using GRK2 antibody at dilution of 1:200 (40x lens).](http://images.novusbio.com/fullsize/GRK2-Antibody-Immunohistochemistry-Paraffin-NBP3-03731-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: GRK2 Antibody [NBP3-03731] - Analysis of extracts of various cell lines, using GRK2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/GRK2-Antibody-Knockout-Validated-NBP3-03731-img0005.jpg)
![Western Blot: VGLUT2 Antibody (S29-29) [NBP2-59330] - Western Blot analysis of Rat brain membrane lysate showing detection of VGLUT2 protein using Mouse Anti-VGLUT2 Monoclonal Antibody, Clone S29-29 (NBP2-59330). Primary Antibody: Mouse Anti-VGLUT2 Monoclonal Antibody (NBP2-59330) at 1:000.](http://images.novusbio.com/fullsize/VGLUT2-Antibody-S29-29-Western-Blot-NBP2-59330-img0004.jpg "Western Blot: VGLUT2 Antibody (S29-29) [NBP2-59330] - Western Blot analysis of Rat brain membrane lysate showing detection of VGLUT2 protein using Mouse Anti-VGLUT2 Monoclonal Antibody, Clone S29-29 (NBP2-59330). Primary Antibody: Mouse Anti-VGLUT2 Monoclonal Antibody (NBP2-59330) at 1:000.")
![Immunocytochemistry/Immunofluorescence: VGLUT2 Antibody (S29-29) [NBP2-59330] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGLUT2 Monoclonal Antibody, Clone S29-29 (NBP2-59330). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-VGLUT2 Monoclonal Antibody (NBP2-59330) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VGLUT2 Antibody (D) Composite.](http://images.novusbio.com/fullsize/VGLUT2-Antibody-S29-29-Immunocytochemistry-Immunofluorescence-NBP2-59330-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: VGLUT2 Antibody (S29-29) [NBP2-59330] - Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VGLUT2 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Secretory Vesicle, Synaptic Vesicle Membrane, Membrane, Cell Junction, Synapse. Magnification: 60X.](http://images.novusbio.com/fullsize/VGLUT2-Antibody-S29-29-Immunocytochemistry-Immunofluorescence-NBP2-59330-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: VGLUT2 Antibody (S29-29) [NBP2-59330] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGLUT2 Monoclonal Antibody, Clone S29-29 (NBP2-59330). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VGLUT2 Monoclonal Antibody (NBP2-59330) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Secretory Vesicle, Synaptic Vesicle Membrane, Membrane, Cell Junction, Synapse. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) VGLUT2 Antibody. (D) Composite.](http://images.novusbio.com/fullsize/VGLUT2-Antibody-S29-29-Immunocytochemistry-Immunofluorescence-NBP2-59330-img0006.jpg)
![Western Blot: GABA-AR alpha 2 Antibody (S399-19) [NBP2-59325] - Western Blot analysis of Rat Brain showing detection of ~55 kDa GABA-AR alpha 2 Alpha 2 protein using Mouse Anti-GABA-AR alpha 2 Alpha 2 Monoclonal Antibody, Clone S399-19 (NBP2-59325). Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 10 ug. Block: 5% Skim Milk for 1 hour at RT. Primary Antibody: Mouse Anti-GABA-AR alpha 2 Alpha 2 Monoclonal Antibody (NBP2-59325) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~55 kDa.](http://images.novusbio.com/fullsize/GABA-AR-alpha-2-Antibody-S399-19-Western-Blot-NBP2-59325-img0008.jpg "Western Blot: GABA-AR alpha 2 Antibody (S399-19) [NBP2-59325] - Western Blot analysis of Rat Brain showing detection of ~55 kDa GABA-AR alpha 2 Alpha 2 protein using Mouse Anti-GABA-AR alpha 2 Alpha 2 Monoclonal Antibody, Clone S399-19 (NBP2-59325). Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 10 ug. Block: 5% Skim Milk for 1 hour at RT. Primary Antibody: Mouse Anti-GABA-AR alpha 2 Alpha 2 Monoclonal Antibody (NBP2-59325) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~55 kDa.")
![Immunocytochemistry/Immunofluorescence: GABA-AR alpha 2 Antibody (S399-19) [NBP2-59325] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Alpha 2 Monoclonal Antibody, Clone S399-19 (NBP2-59325). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha 2 Monoclonal Antibody (NBP2-59325) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-A Receptor Alpha 2 Antibody (D) Composite.](http://images.novusbio.com/fullsize/GABA-AR-alpha-2-Antibody-S399-19-Immunocytochemistry-Immunofluorescence-NBP2-59325-img0009.jpg)
![Western Blot: GABA-AR alpha 2 Antibody (S399-19) [NBP2-59325] - Western Blot analysis of Mouse Brain showing detection of ~55 kDa GABA-AR alpha 2 Alpha 2 protein using Mouse Anti-GABA-AR alpha 2 Alpha 2 Monoclonal Antibody, Clone S399-19 (NBP2-59325). Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 ug. Primary Antibody: Mouse Anti-GABA-AR alpha 2 Alpha 2 Monoclonal Antibody (NBP2-59325) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~55 kDa. Other Band(s): ~ 37 kDa, ~50 kDa, ~70 kDa.](http://images.novusbio.com/fullsize/GABA-AR-alpha-2-Antibody-S399-19-Western-Blot-NBP2-59325-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: GABA-AR alpha 2 Antibody (S399-19) [NBP2-59325] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha2 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/GABA-AR-alpha-2-Antibody-S399-19-Immunocytochemistry-Immunofluorescence-NBP2-59325-img0002.jpg)
![Western Blot: mGluR3 Antibody (6H10C3) [NBP2-61842] - Analysis using GRM3 mAb against HEK293 (1) and GRM3 (AA: extra 433-576)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/mGluR3-Antibody-6H10C3-Western-Blot-NBP2-61842-img0005.jpg "Western Blot: mGluR3 Antibody (6H10C3) [NBP2-61842] - Analysis using GRM3 mAb against HEK293 (1) and GRM3 (AA: extra 433-576)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry: mGluR3 Antibody (6H10C3) [NBP2-61842] - Analysis of paraffin-embedded stomach cancer tissues using GRM3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/mGluR3-Antibody-6H10C3-Immunohistochemistry-NBP2-61842-img0004.jpg)
![Western Blot: mGluR3 Antibody (6H10C3) [NBP2-61842] - Analysis using GRM3 mAb against human GRM3 (AA: extra 433-576) recombinant protein. (Expected MW is 42.4 kDa)](http://images.novusbio.com/fullsize/mGluR3-Antibody-6H10C3-Western-Blot-NBP2-61842-img0006.jpg)
![Immunohistochemistry: mGluR3 Antibody (6H10C3) [NBP2-61842] - Analysis of paraffin-embedded bladder cancer tissues using GRM3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/mGluR3-Antibody-6H10C3-Immunohistochemistry-NBP2-61842-img0003.jpg)
![ELISA: mGluR3 Antibody (6H10C3) [NBP2-61842] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/mGluR3-Antibody-6H10C3-ELISA-NBP2-61842-img0001.jpg)
![ELISA: mGluR3 Antibody (6H10C3) [NBP2-61842] - Analysis of SH-SY5Y cells using GRM3 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/mGluR3-Antibody-6H10C3-ELISA-NBP2-61842-img0002.jpg)
![Western Blot: Kv7.4 Antibody (S43/6) [NBP2-12899] - Western Blot analysis of Rat tissue lysate showing detection of Kv7.4 protein using Mouse Anti-Kv7.4 Monoclonal Antibody, Clone N43/6 (NBP2-12899). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kv7.4 Monoclonal Antibody (NBP2-12899) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/Kv7-4-Antibody-S43-6-Western-Blot-NBP2-12899-img0008.jpg "Western Blot: Kv7.4 Antibody (S43/6) [NBP2-12899] - Western Blot analysis of Rat tissue lysate showing detection of Kv7.4 protein using Mouse Anti-Kv7.4 Monoclonal Antibody, Clone N43/6 (NBP2-12899). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kv7.4 Monoclonal Antibody (NBP2-12899) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: Kv7.4 Antibody (S43/6) [NBP2-12899] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Kv7.4 Monoclonal Antibody, Clone N43/6 (NBP2-12899). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Kv7.4 Monoclonal Antibody (NBP2-12899) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Kv7.4 Antibody (D) Composite.](http://images.novusbio.com/fullsize/Kv7-4-Antibody-S43-6-Immunocytochemistry-Immunofluorescence-NBP2-12899-img0010.jpg)
![Immunohistochemistry: Kv7.4 Antibody (S43/6) [NBP2-12899] - Immunohistochemistry analysis using Mouse Anti-Kv7.4 Monoclonal Antibody, Clone N43/6 (NBP2-12899). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kv7.4 Monoclonal Antibody (NBP2-12899) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/Kv7-4-Antibody-S43-6-Immunohistochemistry-NBP2-12899-img0009.jpg)
![Immunohistochemistry-Frozen: Kv7.4 Antibody (S43/6) [NBP2-12899] - Tissue: frozen brain section. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-KCNQ4 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 uL for 5 minutes at RT.](http://images.novusbio.com/fullsize/Kv7-4-Antibody-S43-6-Immunohistochemistry-Frozen-NBP2-12899-img0006.jpg)
![Western Blot: VAChT/SLC18A3 Antibody (S6-38) [NBP2-59378] - Western Blot analysis of Rat brain membrane lysate showing detection of VAChT/SLC18A3 protein using Mouse Anti-VAChT/SLC18A3 Monoclonal Antibody, Clone S6-38 (NBP2-59378). Primary Antibody: Mouse Anti-VAChT/SLC18A3 Monoclonal Antibody (NBP2-59378) at 1:1000.](http://images.novusbio.com/fullsize/VAChT-SLC18A3-Antibody-S6-38-Western-Blot-NBP2-59378-img0004.jpg "Western Blot: VAChT/SLC18A3 Antibody (S6-38) [NBP2-59378] - Western Blot analysis of Rat brain membrane lysate showing detection of VAChT/SLC18A3 protein using Mouse Anti-VAChT/SLC18A3 Monoclonal Antibody, Clone S6-38 (NBP2-59378). Primary Antibody: Mouse Anti-VAChT/SLC18A3 Monoclonal Antibody (NBP2-59378) at 1:1000.")
![Immunocytochemistry/Immunofluorescence: VAChT/SLC18A3 Antibody (S6-38) [NBP2-59378] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VAChT/SLC18A3 Monoclonal Antibody, Clone S6-38 (NBP2-59378). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-VAChT/SLC18A3 Monoclonal Antibody (NBP2-59378) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VAChT/SLC18A3 Antibody (D) Composite.](http://images.novusbio.com/fullsize/VAChT-SLC18A3-Antibody-S6-38-Immunocytochemistry-Immunofluorescence-NBP2-59378-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: VAChT/SLC18A3 Antibody (S6-38) [NBP2-59378] - Analysis using Mouse Anti-VAChT Monoclonal Antibody, Clone S6-38. Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VAChT Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Membrane. Magnification: 60X.](http://images.novusbio.com/fullsize/VAChT-SLC18A3-Antibody-S6-38-Immunocytochemistry-Immunofluorescence-NBP2-59378-img0003.jpg)
![Immunohistochemistry: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - Immunohistochemistry analysis of rat brain injected with Type 1 mouse alpha synuclein PFFs (NBP2-61596). Species: Female Sprague-Dawley Rat. Rat was injected with 16g Type 1 mouse alpha synuclein PFFs (NBP2-61596) in each of 2 injection sites: AP+1.6, ML+2.4, DV-4.2 from skull; and AP-1.4, ML+0.2, DV-2.8 from skull. 30 days post-injection. Fixation: Saline perfusion followed by 4% PFA fixation for 48 hrs. Primary antibody: rabbit monoclonal anti-pSer129 alpha synuclein. Secondary Antibody: Biotin-SP Donkey Anti-Rabbit IgG (H+L) at 1:500 for 2 hours in cold room with shaking. ABC signal amplification, DAB staining. Magnification: 20X. Alpha synuclein pathology is seen in the periform/insular cortex and the cingulate cortex on both the same (ipsi) and opposite (contra) sides as the injection sites.](http://images.novusbio.com/fullsize/Recombinant-Mouse-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Immunohistochemistry-NBP2-61596-img0003.jpg "Immunohistochemistry: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - Immunohistochemistry analysis of rat brain injected with Type 1 mouse alpha synuclein PFFs (NBP2-61596). Species: Female Sprague-Dawley Rat. Rat was injected with 16g Type 1 mouse alpha synuclein PFFs (NBP2-61596) in each of 2 injection sites: AP+1.6, ML+2.4, DV-4.2 from skull; and AP-1.4, ML+0.2, DV-2.8 from skull. 30 days post-injection. Fixation: Saline perfusion followed by 4% PFA fixation for 48 hrs. Primary antibody: rabbit monoclonal anti-pSer129 alpha synuclein. Secondary Antibody: Biotin-SP Donkey Anti-Rabbit IgG (H+L) at 1:500 for 2 hours in cold room with shaking. ABC signal amplification, DAB staining. Magnification: 20X. Alpha synuclein pathology is seen in the periform/insular cortex and the cingulate cortex on both the same (ipsi) and opposite (contra) sides as the injection sites.")
![In vitro assay: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - Active alpha synuclein aggregate seeds the formation of new alpha Synuclein aggregates from the pool of active monomers. Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha Synuclein aggregates. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate is combined with 100 uM of active alpha Synuclein monomer, as compared to active alpha Synuclein aggregate and active alpha Synuclein monomer alone. Thioflavin T excitation maximum = 450 nm; emission maximum = 485 nm.](http://images.novusbio.com/fullsize/Recombinant-Mouse-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-In-vitro-assay-NBP2-61596-img0002.jpg)
![Electron Microscopy: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - TEM of Type 1 mouse alpha synuclein Pre-formed Fibrils (NBP2-61596). Fibrils were sonicated and image was taken at 100kx magnification.](http://images.novusbio.com/fullsize/Recombinant-Mouse-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Electron-Microscopy-NBP2-61596-img0005.jpg)
![Electron Microscopy: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - TEM of Type 1 mouse alpha synuclein Pre-formed Fibrils (NBP2-61596). Image was taken at 100kx magnification.](http://images.novusbio.com/fullsize/Recombinant-Mouse-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Electron-Microscopy-NBP2-61596-img0004.jpg)
![Immunohistochemistry: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - TH fluorescence in mouse brain. Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp2-61596_recombinant-mouse-alpha-synuclein-active-pre-formed-fibrils-type-1-protein-immunohistochemistry-205202512497..jpg)
![Western Blot: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Western blot analysis of Human, Mouse brain lysate showing detection of ~16 kDa alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human brain lysate. Lane 3: Mouse brain lysate. Lane 4: Alpha Synuclein Monomer (0.5 ug). Load: 15 ug. Block: 2.5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~16 kDa. Other Band(s): 100, 75, 45, 30,16 kDa.](http://images.novusbio.com/fullsize/alpha-Synuclein-[p-Ser129]-Antibody-Western-Blot-NBP2-61121-img0004.jpg "Western Blot: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Western blot analysis of Human, Mouse brain lysate showing detection of ~16 kDa alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human brain lysate. Lane 3: Mouse brain lysate. Lane 4: Alpha Synuclein Monomer (0.5 ug). Load: 15 ug. Block: 2.5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~16 kDa. Other Band(s): 100, 75, 45, 30,16 kDa.")
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Phospho serine 129 antibody (NBP2-61121) was used to detect phosphorylated alpha synuclein in primary mouse hippocampal neurons treated with 100 nM sonicated mouse alpha synuclein PFFs (A). Phosphorylated alpha synuclein was visible in perinucleus and neurites compared to untreated control (B). Read the protocol at pabmabs.com/?p=7901. Image courtesy of Trine Rasmussen, Simon Molgaard Jensen at Aarhus University.](http://images.novusbio.com/fullsize/alpha-Synuclein-[p-Ser129]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-61121-img0006.jpg)
![Immunohistochemistry: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Immunohistochemistry analysis using Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121). Tissue: Brain. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.](http://images.novusbio.com/fullsize/alpha-Synuclein-[p-Ser129]-Antibody-Immunohistochemistry-NBP2-61121-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121). Tissue: Primary hippocampal neurons treated with active Alpha Synuclein Protein Aggregate (SPR-322) at 4 g/ml to induce fibrils. Species: Rat. Fixation: 4% paraformaldehyde. Primary Antibody: Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121) at 1:500 for 24 hours at 4C. Secondary Antibody: Goat Anti-Rabbit Alexa Fluor 488 at 1:700 for 1 hour at RT. Counterstain: Guinea Pig Anti-NeuN (red) neuronal marker (Donkey Anti-Guinea Pig Alexa Fluor 647 1:700); Hoechst (blue) nuclear stain at 1:6000, 1:3000 for 60 min at RT, 5 min at RT. Magnification: 20X.](http://images.novusbio.com/fullsize/alpha-Synuclein [p Ser129] Antibody-Immunocytochemistry-Immunofluorescence-NBP2-61121-img0007.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ER alpha/NR3A1.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI2D3-Azide-and-BSA-Free-Western-Blot-NBP2-70688-img0001.jpg "Western Blot: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ER alpha/NR3A1.")
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of Human breast tissue.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI2D3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70688-img0005.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI2D3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70688-img0002.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of Adenocarcinoma of Human ovary tissue.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI2D3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70688-img0003.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of Carcinoma of Human kidney tissue.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI2D3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70688-img0004.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of Human endometrium tissue.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI2D3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70688-img0006.jpg)