| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-82375SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84826-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56883SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81768-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92715SS | |||
![Western Blot: Mucolipin 1 Antibody [NB110-82375] - Total protein from mouse brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Mucolipin 1 (NB110-82375) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/Mucolipin-1-Antibody-Western-Blot-NB110-82375-img0005.jpg "Western Blot: Mucolipin 1 Antibody [NB110-82375] - Total protein from mouse brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Mucolipin 1 (NB110-82375) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).")
![Simple Western: Mucolipin 1 Antibody [NB110-82375] - Lane view shows a specific band for Mucolipin 1 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Mucolipin-1-Antibody-Simple-Western-NB110-82375-img0004.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Analysis in human endometrium and cerebral cortex tissues. Corresponding ER alpha/NR3A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84826-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Analysis in human endometrium and cerebral cortex tissues. Corresponding ER alpha/NR3A1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Staining of human cerebral cortex, cervix, uterine, endometrium and gastrointestinal using Anti-ER alpha/NR3A1 antibody NBP1-84826 (A) shows similar protein distribution across tissues to independent antibody NBP1-84827 (B).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84826-img0016.jpg)
![Western Blot: ER alpha/NR3A1 Antibody [NBP1-84826] - Analysis in control (vector only transfected HEK293T lysate) and ESR1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Western-Blot-NBP1-84826-img0007.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Staining of human uterine cervix shows strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84826-img0019.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84826-img0014.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Staining of human endometrium shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84826-img0017.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Staining of human small intestine shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84826-img0018.jpg)
![Immunohistochemistry-Paraffin: Gremlin 1 Antibody [NB100-56883] - Detection of Gremlin in human colon cancer tissue using antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/Gremlin-1-Antibody-Immunohistochemistry-Paraffin-NB100-56883-img0002.jpg "Immunohistochemistry-Paraffin: Gremlin 1 Antibody [NB100-56883] - Detection of Gremlin in human colon cancer tissue using antibody at 5 ug/ml.")
![Western Blot: PSMA1 Antibody [NBP1-81768] - Analysis using Anti-PSMA1 antibody NBP1-81768 (A) shows similar pattern to independent antibody NBP2-37908 (B).](http://images.novusbio.com/fullsize/Proteasome-20S-C2-Antibody-Western-Blot-NBP1-81768-img0009.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PSMA1 Antibody [NBP1-81768] - Analysis using Anti-PSMA1 antibody NBP1-81768 (A) shows similar pattern to independent antibody NBP2-37908 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: PSMA1 Antibody [NBP1-81768] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Proteasome-20S-C2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81768-img0007.jpg)
![Immunohistochemistry-Paraffin: PSMA1 Antibody [NBP1-81768] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Proteasome-20S-C2-Antibody-Immunohistochemistry-Paraffin-NBP1-81768-img0011.jpg)
![Western Blot: PSMA1 Antibody [NBP1-81768] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/Proteasome-20S-C2-Antibody-Western-Blot-NBP1-81768-img0006.jpg)
![Immunohistochemistry-Paraffin: PSMA1 Antibody [NBP1-81768] - Staining of human testis shows distinct cytoplasmic and nuclear positivity in cells in seminiferus ducts and Leydig cells.](http://images.novusbio.com/fullsize/Proteasome-20S-C2-Antibody-Immunohistochemistry-Paraffin-NBP1-81768-img0008.jpg)
![Immunohistochemistry-Paraffin: PSMA1 Antibody [NBP1-81768] - Staining of human cervix, uterine shows moderate to strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Proteasome-20S-C2-Antibody-Immunohistochemistry-Paraffin-NBP1-81768-img0010.jpg)
![Immunohistochemistry-Paraffin: PSMA1 Antibody [NBP1-81768] - Staining of human endometrium shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Proteasome-20S-C2-Antibody-Immunohistochemistry-Paraffin-NBP1-81768-img0012.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - Immunoblots of parental HCT116 cells and two clonal cell lines (clone 14 and clone 13) generated by CRISPR-mediated truncation of hRpn10. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-020-15073-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/S5a-Angiocidin-Antibody-Western-Blot-NBP2-19952-img0004.jpg "Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - Immunoblots of parental HCT116 cells and two clonal cell lines (clone 14 and clone 13) generated by CRISPR-mediated truncation of hRpn10. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-020-15073-7), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Proteasome 19S S5A Antibody [NBP2-19952] - Immunofluorescence analysis of paraformaldehyde-fixed A549, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19952-img0002.jpg)
![Western Blot: Proteasome 19S S5A Antibody [NBP2-19952] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Western-Blot-NBP2-19952-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 was knocked down in HCT116 cells by four different siRNAs and the cell lysates immunoprobed as indicated. Mock and scrambled control samples are included. b-actin is used as a loading control. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-020-15073-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/S5a-Angiocidin-Antibody-Knockdown-Validated-NBP2-19952-img0003.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - E6AP levels depend on hRpn10.hRpn10 (a) or E6AP (b) was knocked down in HCT116 cells by four different siRNAs & the cell lysates immunoprobed as indicated. Mock & scrambled control samples are included. beta -actin is used as a loading control in a & d. c Lysates from HCT116 or clone 14 cells expressing myc-hRpn10 constructs were immunoprobed as indicated. d Lysates from HCT116 or clone 13 cells expressing HA-E6AP and/or myc-hRpn10 constructs were immunoprobed as indicated. a–d Antibodies used for immunoprobing are indicated to the left of each panel. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-31020241537198.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - E6AP levels depend on hRpn10.hRpn10 (a) or E6AP (b) was knocked down in HCT116 cells by four different siRNAs & the cell lysates immunoprobed as indicated. Mock & scrambled control samples are included. beta -actin is used as a loading control in a & d. c Lysates from HCT116 or clone 14 cells expressing myc-hRpn10 constructs were immunoprobed as indicated. d Lysates from HCT116 or clone 13 cells expressing HA-E6AP and/or myc-hRpn10 constructs were immunoprobed as indicated. a–d Antibodies used for immunoprobing are indicated to the left of each panel. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-31020241538418.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - E6AP levels depend on hRpn10.hRpn10 (a) or E6AP (b) was knocked down in HCT116 cells by four different siRNAs & the cell lysates immunoprobed as indicated. Mock & scrambled control samples are included. beta -actin is used as a loading control in a & d. c Lysates from HCT116 or clone 14 cells expressing myc-hRpn10 constructs were immunoprobed as indicated. d Lysates from HCT116 or clone 13 cells expressing HA-E6AP and/or myc-hRpn10 constructs were immunoprobed as indicated. a–d Antibodies used for immunoprobing are indicated to the left of each panel. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202415291972.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 RAZUL contributes E6AP to the proteasome.a Immunoblots of Rpt3 immunoprecipitates or WCE from HCT116 or clone 13 lysates expressing myc-hRpn10 constructs. An asterisk “*” indicates heavy chain antibody. Cyclophilin B (Cyp B) is used as a loading control for WCE samples in a–c & hRpn2 as a positive control for the immunoprecipitation. IgG controls are included. b, d Immunoblots of Rpt3 or IgG (control) immunoprecipitates or WCE of lysates from HCT116 cells transfected with empty vector (as a control) or myc-hRpn10 RAZUL. c Immunoblots of Rpt3 immunoprecipitates or WCE from lysates of HCT116 cells transfected with a scrambled control or siRNA against E6AP. a–d Antibodies used for immunoprobing are indicated to the left of each panel. e Pull-down assay for a commercially available mixture of His6-tagged, non-cleavable K48-linked Ub2/Ub4 with incubation of human 26S proteasome (lane 6), 26S proteasome with equimolar E6AP (lane 7), or just E6AP (lane 8). E6AP or 26S proteasome was added to Ni-NTA agarose resin as negative controls (lanes 4 & 5). K48-linked Ub2/Ub4, E6AP, & 26S proteasome were loaded directly in lanes 1–3, as indicated. f Selected regions from 1D 13C-edited, 1H NMR experiments acquired at 850 MHz & 25 °C for free 13C-AZUL (black) or mixtures with equimolar unlabeled RAZUL (blue) or 26S proteasome (red). The concentration of each sample was 0.3 μM & 200,000 scans were recorded for each experiment. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202416113548.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 RAZUL contributes E6AP to the proteasome.a Immunoblots of Rpt3 immunoprecipitates or WCE from HCT116 or clone 13 lysates expressing myc-hRpn10 constructs. An asterisk “*” indicates heavy chain antibody. Cyclophilin B (Cyp B) is used as a loading control for WCE samples in a–c & hRpn2 as a positive control for the immunoprecipitation. IgG controls are included. b, d Immunoblots of Rpt3 or IgG (control) immunoprecipitates or WCE of lysates from HCT116 cells transfected with empty vector (as a control) or myc-hRpn10 RAZUL. c Immunoblots of Rpt3 immunoprecipitates or WCE from lysates of HCT116 cells transfected with a scrambled control or siRNA against E6AP. a–d Antibodies used for immunoprobing are indicated to the left of each panel. e Pull-down assay for a commercially available mixture of His6-tagged, non-cleavable K48-linked Ub2/Ub4 with incubation of human 26S proteasome (lane 6), 26S proteasome with equimolar E6AP (lane 7), or just E6AP (lane 8). E6AP or 26S proteasome was added to Ni-NTA agarose resin as negative controls (lanes 4 & 5). K48-linked Ub2/Ub4, E6AP, & 26S proteasome were loaded directly in lanes 1–3, as indicated. f Selected regions from 1D 13C-edited, 1H NMR experiments acquired at 850 MHz & 25 °C for free 13C-AZUL (black) or mixtures with equimolar unlabeled RAZUL (blue) or 26S proteasome (red). The concentration of each sample was 0.3 μM & 200,000 scans were recorded for each experiment. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202416113543.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 RAZUL contributes E6AP to the proteasome.a Immunoblots of Rpt3 immunoprecipitates or WCE from HCT116 or clone 13 lysates expressing myc-hRpn10 constructs. An asterisk “*” indicates heavy chain antibody. Cyclophilin B (Cyp B) is used as a loading control for WCE samples in a–c & hRpn2 as a positive control for the immunoprecipitation. IgG controls are included. b, d Immunoblots of Rpt3 or IgG (control) immunoprecipitates or WCE of lysates from HCT116 cells transfected with empty vector (as a control) or myc-hRpn10 RAZUL. c Immunoblots of Rpt3 immunoprecipitates or WCE from lysates of HCT116 cells transfected with a scrambled control or siRNA against E6AP. a–d Antibodies used for immunoprobing are indicated to the left of each panel. e Pull-down assay for a commercially available mixture of His6-tagged, non-cleavable K48-linked Ub2/Ub4 with incubation of human 26S proteasome (lane 6), 26S proteasome with equimolar E6AP (lane 7), or just E6AP (lane 8). E6AP or 26S proteasome was added to Ni-NTA agarose resin as negative controls (lanes 4 & 5). K48-linked Ub2/Ub4, E6AP, & 26S proteasome were loaded directly in lanes 1–3, as indicated. f Selected regions from 1D 13C-edited, 1H NMR experiments acquired at 850 MHz & 25 °C for free 13C-AZUL (black) or mixtures with equimolar unlabeled RAZUL (blue) or 26S proteasome (red). The concentration of each sample was 0.3 μM & 200,000 scans were recorded for each experiment. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-31020241611358.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 RAZUL contributes E6AP to the proteasome.a Immunoblots of Rpt3 immunoprecipitates or WCE from HCT116 or clone 13 lysates expressing myc-hRpn10 constructs. An asterisk “*” indicates heavy chain antibody. Cyclophilin B (Cyp B) is used as a loading control for WCE samples in a–c & hRpn2 as a positive control for the immunoprecipitation. IgG controls are included. b, d Immunoblots of Rpt3 or IgG (control) immunoprecipitates or WCE of lysates from HCT116 cells transfected with empty vector (as a control) or myc-hRpn10 RAZUL. c Immunoblots of Rpt3 immunoprecipitates or WCE from lysates of HCT116 cells transfected with a scrambled control or siRNA against E6AP. a–d Antibodies used for immunoprobing are indicated to the left of each panel. e Pull-down assay for a commercially available mixture of His6-tagged, non-cleavable K48-linked Ub2/Ub4 with incubation of human 26S proteasome (lane 6), 26S proteasome with equimolar E6AP (lane 7), or just E6AP (lane 8). E6AP or 26S proteasome was added to Ni-NTA agarose resin as negative controls (lanes 4 & 5). K48-linked Ub2/Ub4, E6AP, & 26S proteasome were loaded directly in lanes 1–3, as indicated. f Selected regions from 1D 13C-edited, 1H NMR experiments acquired at 850 MHz & 25 °C for free 13C-AZUL (black) or mixtures with equimolar unlabeled RAZUL (blue) or 26S proteasome (red). The concentration of each sample was 0.3 μM & 200,000 scans were recorded for each experiment. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202416121996.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - A C-terminal domain in hRpn10 binds E6AP AZUL.a Positions of known functional domains within hRpn10 (top) & E6AP isoform II (bottom). Question mark “?” indicates hRpn10 uncharacterized region & the E6AP catalytic cysteine C843 is indicated. b Pull-down assay of His-tagged hRpn10full-length (full), hRpn10196–377 or hRpn10196–306 without (−) or with (+) incubation of E6AP. c1H, 15N HSQC spectra of 0.2 mM 15N-hRpn10305–377 (black) & with twofold molar excess unlabeled AZUL (green). Shifted signals are labeled. d Table summarizing Kd, kon, & koff average values with standard deviations for the hRpn10305–377: AZUL interaction measured by ITC and/or SPR. N/A, not applicable. e HCT116 lysates expressing empty vector, myc-hRpn10 full length, or myc-Rpn10 with RAZUL deleted ( delta 307–377) were subjected to myc-immunoprecipitation with anti-myc-tag nanobody-coupled agarose. Whole cell extracts (WCE) & myc-immunoprecipitates were immunoprobed with the indicated antibodies. Cyclophilin B (Cyp B) is used as a loading control in e & f. f Lysates from HCT116 cells expressing HA-E6AP & the myc-hRpn10 constructs of e were subjected to HA IP followed by immunoblotting with the indicated antibodies. An asterisk “*” indicates non-specific interaction; double asterisk “**” indicates heavy chain antibody. e–f All antibodies used for immunoprobing are indicated to the left of the images. Note that the hRpn10 & E6AP antibodies recognize both endogenous & exogenously expressed protein, causing these panels to show both tagged & endogenous protein. g Schematic representation highlighting interaction domains of hRpn10 including newly identified RAZUL. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202416114714.jpg)
![Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555] - Mouse pancreas. NAGLU antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-N-acetylglucosaminidase-NAGLU-Antibody-Immunohistochemistry-Paraffin-NBP1-31555-img0012.jpg "Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555] - Mouse pancreas. NAGLU antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunocytochemistry/Immunofluorescence: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NAGLU protein stained by NAGLU antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/alpha-N-acetylglucosaminidase-NAGLU-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31555-img0001.jpg)
![Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555] - Mouse pancreas. NAGLU antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-N-acetylglucosaminidase-NAGLU-Antibody-Immunohistochemistry-Paraffin-NBP1-31555-img0009.jpg)
![Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555] - Rat duodenum. NAGLU antibody diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-N-acetylglucosaminidase-NAGLU-Antibody-Immunohistochemistry-Paraffin-NBP1-31555-img0010.jpg)
![Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555] - Mouse brain. NAGLU antibody diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-N-acetylglucosaminidase-NAGLU-Antibody-Immunohistochemistry-Paraffin-NBP1-31555-img0011.jpg)
![alpha-N-acetylglucosaminidase/NAGLU antibody detects alpha-N-acetylglucosaminidase/NAGLU protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Green: alpha-N-acetylglucosaminidase/NAGLU antibody (NBP1-31555) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) .Blue: Nuclear staining with Hoechst 33342. ^[^GTX]br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31555_rabbit-polyclonal-alpha-n-acetylglucosaminidase-naglu-antibody-immunohistochemistry-paraffin-141120251843564.jpg)
![alpha-N-acetylglucosaminidase/NAGLU antibody detects alpha-N-acetylglucosaminidase/NAGLU Protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse cerebellum. Green: alpha-N-acetylglucosaminidase/NAGLU stained by alpha-N-acetylglucosaminidase/NAGLU antibody (NBP1-31555) diluted at 1:250.Red: NF-H, stained by NF-H antibody [GT114] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 10 min](http://images.novusbio.com/fullsize/nbp1-32244_rabbit-polyclonal-calretinin-antibody-immunohistochemistry-frozen-141120251855418.jpg)
![Western Blot: Park7/DJ-1 Antibody (4H4) [NBP1-92715] - Western blot shows lysates of 293T human embryonic kidney parental cell line and PARK7 knockout (KO) 293T cell line. PVDF membrane was probed with Mouse Anti-Human PARK7 Monoclonal Antibody (Catalog # NBP1-92715) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for PARK7 at approximately 23 kDa (as indicated) in the parental 293T cell line, but is not detectable in the knockout 293T cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Park7-DJ-1-Antibody-4H4-Knockout-Validated-NBP1-92715-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Park7/DJ-1 Antibody (4H4) [NBP1-92715] - Western blot shows lysates of 293T human embryonic kidney parental cell line and PARK7 knockout (KO) 293T cell line. PVDF membrane was probed with Mouse Anti-Human PARK7 Monoclonal Antibody (Catalog # NBP1-92715) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for PARK7 at approximately 23 kDa (as indicated) in the parental 293T cell line, but is not detectable in the knockout 293T cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Park7/DJ-1 Antibody (4H4) [NBP1-92715] - Analysis of whole brain and cell lysates using mouse mAb against DJ1/Park7, NBP1-92715, dilution 1:5,000 in green. [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HeLa, [6] HEK293, and [7] C6 cells. The DJ1 antibody detects protein with apparent molecular weight of 21kDa but only in human cell lines, since it does not recognize the rat or mouse DJ1 protein. The blot was simultaneously probed with chicken pAb to vimentin, dilution 1;5,000 in red, revealing a single band at about 50kDa present in all lanes, though at much lower levels in the tissue lysates.](http://images.novusbio.com/fullsize/Park7-DJ-1-Antibody-4H4-Western-Blot-NBP1-92715-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Park7(DJ-1) Antibody (4H4) [NBP1-92715] - HeLa cells stained with NBP1-92715 (green), chicken antibody to Vimentin (NB300-223, red) and DNA (blue). NBP1-92715 reveals strong cytoplasmic staining for Park7(DJ-1).](http://images.novusbio.com/fullsize/Park7-DJ-1-Antibody-4H4-Immunocytochemistry-Immunofluorescence-NBP1-92715-img0003.jpg)
![Simple Western: Park7/DJ-1 Antibody (4H4) [NBP1-92715] - Lane view shows a specific band for Park7 (DJ-1) in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Park7-DJ-1-Antibody-4H4-Simple-Western-NBP1-92715-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: IP3R1 Antibody (HL1541) - Azide and BSA Free [NBP3-25535] - IP3 Receptor I antibody [HL1541] detects IP3 Receptor I protein at endoplasmic reticulum by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IP3 Receptor I stained by IP3 Receptor I antibody [HL1541] (NBP3-25535) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25535_rabbit-ip3r1-mab-hl1541-azide-and-bsa-free-212024940874.jpg "Immunocytochemistry/Immunofluorescence: IP3R1 Antibody (HL1541) - Azide and BSA Free [NBP3-25535] - IP3 Receptor I antibody [HL1541] detects IP3 Receptor I protein at endoplasmic reticulum by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IP3 Receptor I stained by IP3 Receptor I antibody [HL1541] (NBP3-25535) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Western Blot: IP3R1 Antibody (HL1541) - Azide and BSA Free [NBP3-25535] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with IP3 Receptor I antibody [HL1541] (NBP3-25535) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25535_rabbit-ip3r1-mab-hl1541-azide-and-bsa-free-2120249371512.jpg)
![Western Blot: IP3R1 Antibody (HL1541) - Azide and BSA Free [NBP3-25535] - Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with IP3 Receptor I antibody [HL1541] (NBP3-25535) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25535_rabbit-ip3r1-mab-hl1541-azide-and-bsa-free-2120249432830.jpg)
![Western Blot: IP3R1 Antibody (HL1541) - Azide and BSA Free [NBP3-25535] - Whole cell extract (30 Aug) was separated by 5% SDS-PAGE, and the membrane was blotted with IP3 Receptor I antibody [HL1541] (NBP3-25535) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25535_rabbit-ip3r1-mab-hl1541-azide-and-bsa-free-2120249371526.jpg)
![Immunohistochemistry-Paraffin: IP3R1 Antibody (HL1541) - Azide and BSA Free [NBP3-25535] - IP3 Receptor I antibody [HL1541] detects IP3 Receptor I protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. IP3 Receptor I stained by IP3 Receptor I antibody [HL1541] (NBP3-25535) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25535_rabbit-ip3r1-mab-hl1541-azide-and-bsa-free-2120249425457.jpg)
![Immunohistochemistry-Paraffin: IP3R1 Antibody (HL1541) - Azide and BSA Free [NBP3-25535] - IP3 Receptor I antibody [HL1541] detects IP3 Receptor I protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. IP3 Receptor I stained by IP3 Receptor I antibody [HL1541] (NBP3-25535) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25535_rabbit-ip3r1-mab-hl1541-azide-and-bsa-free-2120249362765.jpg)
![Immunohistochemistry-Paraffin: IP3R1 Antibody (HL1541) - Azide and BSA Free [NBP3-25535] - IP3 Receptor I antibody [HL1541] detects IP3 Receptor I protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. IP3 Receptor I stained by IP3 Receptor I antibody [HL1541] (NBP3-25535) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25535_rabbit-ip3r1-mab-hl1541-azide-and-bsa-free-212024940889.jpg)
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with IP3R1 antibody [HL1541] (NBP3-25535) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25535_rabbit-ip3r1-mab-hl1541-azide-and-bsa-free-western-blot-141120251844343.jpg)
![Western Blot: GluR2 Antibody (9R1X2) [NBP3-15370] - Analysis of extracts of various cell lines, using GluR2 Rabbit mAb (NBP3-15370) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/GluR2-Antibody-9R1X2-Western-Blot-NBP3-15370-img0006.jpg "Western Blot: GluR2 Antibody (9R1X2) [NBP3-15370] - Analysis of extracts of various cell lines, using GluR2 Rabbit mAb (NBP3-15370) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: GluR2 Antibody (9R1X2) [NBP3-15370] - Immunofluorescence analysis of SH-SY5Y cells using GluR2 Rabbit mAb (NBP3-15370) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/GluR2-Antibody-9R1X2-Immunocytochemistry-Immunofluorescence-NBP3-15370-img0003.jpg)
![Immunohistochemistry: GluR2 Antibody (9R1X2) [NBP3-15370] - Immunofluorescence analysis of mouse brain using GluR2 Rabbit mAb (NBP3-15370) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/GluR2-Antibody-9R1X2-Immunohistochemistry-NBP3-15370-img0005.jpg)
![Immunohistochemistry-Paraffin: GluR2 Antibody (9R1X2) [NBP3-15370] - Rat brain using GluR2 Rabbit mAb (NBP3-15370) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/GluR2-Antibody-9R1X2-Immunohistochemistry-Paraffin-NBP3-15370-img0001.jpg)
![Immunohistochemistry-Paraffin: GluR2 Antibody (9R1X2) [NBP3-15370] - Mouse brain using GluR2 Rabbit mAb (NBP3-15370) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/GluR2-Antibody-9R1X2-Immunohistochemistry-Paraffin-NBP3-15370-img0002.jpg)
![Immunohistochemistry: GluR2 Antibody (9R1X2) [NBP3-15370] - Immunofluorescence analysis of rat brain using GluR2 Rabbit mAb (NBP3-15370) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/GluR2-Antibody-9R1X2-Immunohistochemistry-NBP3-15370-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: GluR2 Antibody (9R1X2) [GluR2] - Confocal imaging of paraffin-embedded Rat brain tissue using GluR2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15370_rabbit-glur2-mab-9r1x2-52202517162341.jpg)
![Immunocytochemistry/ Immunofluorescence: GluR2 Antibody (9R1X2) [GluR2] - Confocal imaging of paraffin-embedded Mouse brain tissue using GluR2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15370_rabbit-glur2-mab-9r1x2-52202517172841.jpg)
![Western Blot: VAMP-1 Antibody (1A3K6) [NBP3-16212] - Western blot analysis of extracts of various cell lines, using VAMP-1 Rabbit mAb (NBP3-16212) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/VAMP-1-Antibody-1A3K6-Western-Blot-NBP3-16212-img0007.jpg "Western Blot: VAMP-1 Antibody (1A3K6) [NBP3-16212] - Western blot analysis of extracts of various cell lines, using VAMP-1 Rabbit mAb (NBP3-16212) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/ Immunofluorescence: VAMP-1 Antibody (1A3K6) [NBP3-16212] - Immunofluorescence analysis of U-2 OS cells using VAMP-1 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16212_rabbit-vamp-1-mab-1a3k6-72202512443367.jpg)
![Immunohistochemistry: VAMP-1 Antibody (1A3K6) [NBP3-16212] - Immunohistochemistry analysis of VAMP-1 in paraffin-embedded mouse brain tissue using VAMP-1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16212_rabbit-vamp-1-mab-1a3k6-7220251244330.jpg)
![Immunohistochemistry: VAMP-1 Antibody (1A3K6) [NBP3-16212] - Immunohistochemistry analysis of VAMP-1 in paraffin-embedded mouse colon tissue using VAMP-1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16212_rabbit-vamp-1-mab-1a3k6-7220251245355.jpg)
![Immunohistochemistry: VAMP-1 Antibody (1A3K6) [NBP3-16212] - Immunohistochemistry analysis of VAMP-1 in paraffin-embedded rat brain tissue using VAMP-1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16212_rabbit-vamp-1-mab-1a3k6-72202512435380.jpg)
![Immunohistochemistry: VAMP-1 Antibody (1A3K6) [NBP3-16212] - Immunohistochemistry analysis of VAMP-1 in paraffin-embedded human brain tissue using VAMP-1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16212_rabbit-vamp-1-mab-1a3k6-72202512432096.jpg)
![Immunocytochemistry/ Immunofluorescence: VAMP-1 Antibody (1A3K6) [NBP3-16212] - Immunofluorescence analysis of C6 cells using VAMP-1 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16212_rabbit-vamp-1-mab-1a3k6-72202512443348.jpg)
![Immunocytochemistry/ Immunofluorescence: VAMP-1 Antibody (1A3K6) [NBP3-16212] - Immunofluorescence analysis of NIH-3T3 cells using VAMP-1 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16212_rabbit-vamp-1-mab-1a3k6-8220252010410.jpg)
![Western Blot: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Analysis of extracts of various cell lines, using Glutamate Dehydrogenase Rabbit mAb (NBP3-16582) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-7L7N2-Western-Blot-NBP3-16582-img0005.jpg "Western Blot: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Analysis of extracts of various cell lines, using Glutamate Dehydrogenase Rabbit mAb (NBP3-16582) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Immunofluorescence analysis of NIH-3T3 cells using Glutamate Dehydrogenase Rabbit mAb (NBP3-16582) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-7L7N2-Immunocytochemistry-Immunofluorescence-NBP3-16582-img0004.jpg)
![Western Blot: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Analysis of extracts of various cell lines, using Glutamate Dehydrogenase Rabbit mAb (NBP3-16582) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-7L7N2-Western-Blot-NBP3-16582-img0006.jpg)
![Immunohistochemistry: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Glutamate Dehydrogenase Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16582_rabbit-glutamate-dehydrogenase-mab-7l7n2-52202511383740.jpg)
![Immunohistochemistry: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using Glutamate Dehydrogenase Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16582_rabbit-glutamate-dehydrogenase-mab-7l7n2-522025125432.jpg)
![Immunohistochemistry: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Glutamate Dehydrogenase Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16582_rabbit-glutamate-dehydrogenase-mab-7l7n2-52202511361270.jpg)
![Immunohistochemistry: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Glutamate Dehydrogenase Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16582_rabbit-glutamate-dehydrogenase-mab-7l7n2-52202511411443.jpg)
![Immunohistochemistry: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using Glutamate Dehydrogenase Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16582_rabbit-glutamate-dehydrogenase-mab-7l7n2-5220251215457.jpg)
![Immunohistochemistry: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using Glutamate Dehydrogenase Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16582_rabbit-glutamate-dehydrogenase-mab-7l7n2-52202511361223.jpg)
![Immunohistochemistry: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using Glutamate Dehydrogenase Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16582_rabbit-glutamate-dehydrogenase-mab-7l7n2-52202511411490.jpg)
![Immunohistochemistry: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Glutamate Dehydrogenase Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16582_rabbit-glutamate-dehydrogenase-mab-7l7n2-52202511365186.jpg)
![Western Blot: USP9x Antibody (JG35-11) [NBP2-75704] - Analysis of USP9X on different lysates using anti-USP9X antibody at 1/1,000 dilution.Positive control:Lane 1: SiHa Lane 2: A549Lane 3: 293 Lane 4: Mouse colon](http://images.novusbio.com/fullsize/USP9x-Antibody-JG35-11-Western-Blot-NBP2-75704-img0007.jpg "Western Blot: USP9x Antibody (JG35-11) [NBP2-75704] - Analysis of USP9X on different lysates using anti-USP9X antibody at 1/1,000 dilution.Positive control:Lane 1: SiHa Lane 2: A549Lane 3: 293 Lane 4: Mouse colon")
![Immunocytochemistry/Immunofluorescence: USP9x Antibody (JG35-11) [NBP2-75704] - Staining USP9X in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/USP9x-Antibody-JG35-11-Immunocytochemistry-Immunofluorescence-NBP2-75704-img0004.jpg)
![Immunohistochemistry-Paraffin: USP9x Antibody (JG35-11) [NBP2-75704] - Analysis of paraffin-embedded mouse fallopian tube tissue using anti-USP9X antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.](http://images.novusbio.com/fullsize/USP9x-Antibody-JG35-11-Immunohistochemistry-Paraffin-NBP2-75704-img0006.jpg)
![Flow Cytometry: USP9x Antibody (JG35-11) [NBP2-75704] - Analysis of 293T cells with USP9X antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/USP9x-Antibody-JG35-11-Flow-Cytometry-NBP2-75704-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: USP9x Antibody (JG35-11) [NBP2-75704] - Staining USP9X in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/USP9x-Antibody-JG35-11-Immunocytochemistry-Immunofluorescence-NBP2-75704-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: USP9x Antibody (JG35-11) [NBP2-75704] - Staining USP9X in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/USP9x-Antibody-JG35-11-Immunocytochemistry-Immunofluorescence-NBP2-75704-img0003.jpg)
![Immunohistochemistry-Paraffin: USP9x Antibody (JG35-11) [NBP2-75704] - Analysis of paraffin-embedded human tonsil tissue using anti-USP9X antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.](http://images.novusbio.com/fullsize/USP9x-Antibody-JG35-11-Immunohistochemistry-Paraffin-NBP2-75704-img0005.jpg)