| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59057 | |||
| BSA Free | NBP2-59057-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90589-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88972-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38564-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86674-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37465-0.025ml | |||
![Western Blot: TRIOBP Antibody [NBP1-90589] - Analysis in human cell lines A-431 and Caco-2. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.](http://images.novusbio.com/fullsize/TRIOBP-Antibody-Western-Blot-NBP1-90589-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: TRIOBP Antibody [NBP1-90589] - Analysis in human cell lines A-431 and Caco-2. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: TRIOBP Antibody [NBP1-90589] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/TRIOBP-Antibody-Immunohistochemistry-Paraffin-NBP1-90589-img0013.jpg)
![Immunohistochemistry-Paraffin: TRIOBP Antibody [NBP1-90589] - Staining of human endometrium shows strong cytoplasmic positivity in stromal cells.](http://images.novusbio.com/fullsize/TRIOBP-Antibody-Immunohistochemistry-Paraffin-NBP1-90589-img0010.jpg)
![Immunohistochemistry-Paraffin: TRIOBP Antibody [NBP1-90589] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/TRIOBP-Antibody-Immunohistochemistry-Paraffin-NBP1-90589-img0011.jpg)
![Immunohistochemistry-Paraffin: TRIOBP Antibody [NBP1-90589] - Staining of human prostate shows weak cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/TRIOBP-Antibody-Immunohistochemistry-Paraffin-NBP1-90589-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: TET1 Antibody [NBP1-88972] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear membrane.](http://images.novusbio.com/fullsize/TET1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88972-img0005.jpg "Immunocytochemistry/Immunofluorescence: TET1 Antibody [NBP1-88972] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear membrane.")
![Immunohistochemistry-Paraffin: TET1 Antibody [NBP1-88972] - Staining of human placenta shows moderate nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/TET1-Antibody-Immunohistochemistry-Paraffin-NBP1-88972-img0009.jpg)
![Immunohistochemistry-Paraffin: TET1 Antibody [NBP1-88972] - Staining of human cerebellum shows moderate nuclear positivity in purkinje cells.](http://images.novusbio.com/fullsize/TET1-Antibody-Immunohistochemistry-Paraffin-NBP1-88972-img0006.jpg)
![Immunohistochemistry-Paraffin: TET1 Antibody [NBP1-88972] - Staining of human cervix, uterine shows moderate nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/TET1-Antibody-Immunohistochemistry-Paraffin-NBP1-88972-img0007.jpg)
![Immunohistochemistry-Paraffin: TET1 Antibody [NBP1-88972] - Staining of human kidney shows moderate nuclear positivity in cells in tubules.](http://images.novusbio.com/fullsize/TET1-Antibody-Immunohistochemistry-Paraffin-NBP1-88972-img0008.jpg)
![Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564] - Staining of human cerebellum shows moderate nuclear positivity in all layers.](http://images.novusbio.com/fullsize/SIN3A-Antibody-Immunohistochemistry-Paraffin-NBP2-38564-img0011.jpg "Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564] - Staining of human cerebellum shows moderate nuclear positivity in all layers.")
![Immunohistochemistry: SIN3A Antibody [NBP2-38564] - Staining of human testis shows nuclear positivity in seminiferus ducts.](http://images.novusbio.com/fullsize/SIN3A-Antibody-Immunohistochemistry-NBP2-38564-img0002.jpg)
![Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/SIN3A-Antibody-Immunohistochemistry-Paraffin-NBP2-38564-img0003.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564] - Staining in human testis and liver tissues using anti-SIN3A antibody. Corresponding SIN3A RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SIN3A-Antibody-Immunohistochemistry-Paraffin-NBP2-38564-img0004.jpg)
![Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564] - Staining of human skin.](http://images.novusbio.com/fullsize/SIN3A-Antibody-Immunohistochemistry-Paraffin-NBP2-38564-img0006.jpg)
![Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564] - Staining of human kidney.](http://images.novusbio.com/fullsize/SIN3A-Antibody-Immunohistochemistry-Paraffin-NBP2-38564-img0007.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564] - Staining of human cerebellum, kidney, liver and testis using Anti-SIN3A antibody NBP2-38564 (A) shows similar protein distribution across tissues to independent antibody NBP2-38949 (B).](http://images.novusbio.com/fullsize/SIN3A-Antibody-Immunohistochemistry-Paraffin-NBP2-38564-img0008.jpg)
![Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/SIN3A-Antibody-Immunohistochemistry-Paraffin-NBP2-38564-img0009.jpg)
![Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564] - Staining of human kidney shows moderate to strong nuclear positivity in cells in tubules.](http://images.novusbio.com/fullsize/SIN3A-Antibody-Immunohistochemistry-Paraffin-NBP2-38564-img0010.jpg)
![Immunohistochemistry-Paraffin: SIN3A Antibody [NBP2-38564] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/SIN3A-Antibody-Immunohistochemistry-Paraffin-NBP2-38564-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: MAML3 Antibody [NBP1-86674] - Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles.](http://images.novusbio.com/fullsize/MAML3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86674-img0006.jpg "Immunocytochemistry/Immunofluorescence: MAML3 Antibody [NBP1-86674] - Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles.")
![Immunohistochemistry-Paraffin: MAML3 Antibody [NBP1-86674] - Analysis in human endometrium and skeletal muscle tissues using NBP1-86674 antibody. Corresponding MAML3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MAML3-Antibody-Immunohistochemistry-Paraffin-NBP1-86674-img0007.jpg)
![Immunohistochemistry-Paraffin: MAML3 Antibody [NBP1-86674] - Staining of human Skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/MAML3-Antibody-Immunohistochemistry-Paraffin-NBP1-86674-img0011.jpg)
![Immunohistochemistry-Paraffin: MAML3 Antibody [NBP1-86674] - Staining of human Endometrium shows moderate nuclear positivity in stromal and glandular cells.](http://images.novusbio.com/fullsize/MAML3-Antibody-Immunohistochemistry-Paraffin-NBP1-86674-img0009.jpg)
![Immunohistochemistry-Paraffin: MAML3 Antibody [NBP1-86674] - Staining of human Cervix shows moderate nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/MAML3-Antibody-Immunohistochemistry-Paraffin-NBP1-86674-img0008.jpg)
![Immunohistochemistry-Paraffin: MAML3 Antibody [NBP1-86674] - Staining of human Lymph node shows moderate nuclear positivity in germinal center and non-germinal center cells.](http://images.novusbio.com/fullsize/MAML3-Antibody-Immunohistochemistry-Paraffin-NBP1-86674-img0010.jpg)
![Immunohistochemistry-Paraffin: MAML3 Antibody [NBP1-86674] - Staining of human Testis shows moderate nuclear positivity in cells in seminiferous ducts and Leydig cells.](http://images.novusbio.com/fullsize/MAML3-Antibody-Immunohistochemistry-Paraffin-NBP1-86674-img0012.jpg)
![Western Blot: Enolase 2/Neuron-specific Enolase Antibody (5D3) [NBP2-37465] - Analysis using ENO2 mouse mAb against Mouse brain (1), NIH3T3 (2), and C6 (3) cell lysate.](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-5D3-Western-Blot-NBP2-37465-img0003.jpg "Western Blot: Enolase 2/Neuron-specific Enolase Antibody (5D3) [NBP2-37465] - Analysis using ENO2 mouse mAb against Mouse brain (1), NIH3T3 (2), and C6 (3) cell lysate.")
![Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (5D3) [NBP2-37465] - Analysis of liver cancer tissues using ENO2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-5D3-Immunohistochemistry-NBP2-37465-img0006.jpg)
![Flow Cytometry: Enolase 2/Neuron-specific Enolase Antibody (5D3) [NBP2-37465] - Analysis of HeLa cells using ENO2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-5D3-Flow-Cytometry-NBP2-37465-img0004.jpg)
![Western Blot: Enolase 2/Neuron-specific Enolase Antibody (5D3) [NBP2-37465] - Analysis using ENO2 mAb against human ENO2 recombinant protein. (Expected MW is 46 kDa)](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-5D3-Western-Blot-NBP2-37465-img0002.jpg)
![Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (5D3) [NBP2-37465] - Analysis of lung cancer tissues using ENO2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-5D3-Immunohistochemistry-NBP2-37465-img0005.jpg)
![ELISA: Enolase 2/Neuron-specific Enolase Antibody (5D3) [NBP2-37465] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-5D3-ELISA-NBP2-37465-img0001.jpg)
![Western Blot: CRABP2 Antibody [NBP2-24764] - Analysis in HCT116 cell lysate in the 1) absence and 2) presence of immunizing peptide. Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/CRABP2-Antibody-Western-Blot-NBP2-24764-img0002.jpg "Western Blot: CRABP2 Antibody [NBP2-24764] - Analysis in HCT116 cell lysate in the 1) absence and 2) presence of immunizing peptide. Dilution: 1.0 ug/ml.")
![Immunohistochemistry-Paraffin: CRABP2 Antibody [NBP2-24764] - Staining of human liver tissue using an isotype control (left) and this antibody (right) at 5 ug/ml.](http://images.novusbio.com/fullsize/CRABP2-Antibody-Immunohistochemistry-Paraffin-NBP2-24764-img0001.jpg)
![Western Blot: CRABP2 Antibody [NBP2-15998] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with CRABP2 antibody [N1C3] diluted at 1:1000.](http://images.novusbio.com/fullsize/CRABP2-Antibody-Western-Blot-NBP2-15998-img0005.jpg "Western Blot: CRABP2 Antibody [NBP2-15998] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with CRABP2 antibody [N1C3] diluted at 1:1000.")
![Western Blot: CRABP2 Antibody [NBP2-15998] - Sample (30 ug of whole cell lysate) A: Hep G2 15% SDS PAGE NBP2-15998 diluted at 1:1000.](http://images.novusbio.com/fullsize/CRABP2-Antibody-Western-Blot-NBP2-15998-img0001.jpg)
![Immunohistochemistry-Paraffin: CRABP2 Antibody [NBP2-15998] - Immunohistochemical analysis of paraffin-embedded MDAMB468 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/CRABP2-Antibody-Immunohistochemistry-Paraffin-NBP2-15998-img0002.jpg)
![CRABP2 antibody [N1C3] detects CRABP2 protein expression by immunohistochemical analysis.Sample:Paraffin-Embedded adult mouse retina. Green: CRABP2 protein stained by CRABP2 antibody [N1C3] (NBP2-15998) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-15998_rabbit-polyclonal-crabp2-antibody-immunohistochemistry-paraffin-261120251847573.jpg)
![CRABP2 antibody [N1C3] detects CRABP2 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CRABP2 protein stained by CRABP2 antibody [N1C3] (NBP2-15998) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-15998_rabbit-polyclonal-crabp2-antibody-immunocytochemistry-immunofluorescence-261120251849134.jpg)
![Western Blot: MAML3 Antibody [NB100-2129] - Detection of Human and Mouse MAML3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MAML3 antibody NB100-2129 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/MAML3-Antibody-Western-Blot-NB100-2129-img0005.jpg "Western Blot: MAML3 Antibody [NB100-2129] - Detection of Human and Mouse MAML3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MAML3 antibody NB100-2129 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: MAML3 Antibody [NB100-2129] - Detection of human MAML3 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-MAML3 (NB100-2129). Detection: DAB](http://images.novusbio.com/fullsize/MAML3-Antibody-Immunohistochemistry-NB100-2129-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: MAML3 Antibody [NB100-2129] - Detection of human MAML3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MAML3 antibody NB100-2129 (lot NB100-2129-2) used for IP at 6 ug per reaction. MAML3 was also immunoprecipitated by a previous lot of this antibody (lot NB100-2129-1) and rabbit anti-MAML3 antibody NB100-2128 For blotting immunoprecipitated MAML3, NB100-2129 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/MAML3-Antibody-Immunoprecipitation-NB100-2129-img0007.jpg)
![Immunohistochemistry-Frozen: Enolase 2/Neuron-specific Enolase Antibody [NBP1-32090] - Frozen sectioned E13.5 Rat brain. Green: NSE protein stained by NSE antibody [N1C1] diluted at 1:250. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-Immunohistochemistry-Frozen-NBP1-32090-img0006.jpg "Immunohistochemistry-Frozen: Enolase 2/Neuron-specific Enolase Antibody [NBP1-32090] - Frozen sectioned E13.5 Rat brain. Green: NSE protein stained by NSE antibody [N1C1] diluted at 1:250. Blue: Fluoroshield with DAPI.")
![Western Blot: Enolase 1 Antibody [NBP1-32090] - Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Western-Blot-NBP1-32090-img0003.jpg)
![Immunohistochemistry-Paraffin: Enolase 1 Antibody [NBP1-32090] - Paraffin-embedded PC14 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Immunohistochemistry-Paraffin-NBP1-32090-img0002.jpg)
![Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NBP1-32090] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Enolase 2/Neuron-specific Enolase antibody [N1C1] (NBP1-32090) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32090_rabbit-polyclonal-enolase-2-neuron-specific-enolase-antibody-9102024205620.jpg)
![Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NBP1-32090] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Enolase 2/Neuron-specific Enolase antibody [N1C1] (NBP1-32090) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32090_rabbit-polyclonal-enolase-2-neuron-specific-enolase-antibody-91020242041213.jpg)
![Western Blot: Enolase 2/Neuron-specific Enolase Antibody (9B4Y4) [Enolase 2/Neuron-specific Enolase] - Western blot analysis of various lysates using Enolase 2/Neuron-specific Enolase Rabbit mAb at 1:10000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Negative control (NC): U-937Exposure time: 15s.](http://images.novusbio.com/fullsize/nbp3-15659_rabbit-enolase-2-neuron-specific-enolase-mab-9b4y4-52202517315564.jpg "Western Blot: Enolase 2/Neuron-specific Enolase Antibody (9B4Y4) [Enolase 2/Neuron-specific Enolase] - Western blot analysis of various lysates using Enolase 2/Neuron-specific Enolase Rabbit mAb at 1:10000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Negative control (NC): U-937Exposure time: 15s.")
![Immunocytochemistry/ Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody (9B4Y4) [Enolase 2/Neuron-specific Enolase] - Confocal imaging of Neuro-2a cells using Enolase 2/Neuron-specific Enolase Rabbit mAb . The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15659_rabbit-enolase-2-neuron-specific-enolase-mab-9b4y4-5220251733389.jpg)
![Immunohistochemistry: Enolase 2/Neuron-specific Enolase Antibody (9B4Y4) [Enolase 2/Neuron-specific Enolase] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Enolase 2/Neuron-specific Enolase Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15659_rabbit-enolase-2-neuron-specific-enolase-mab-9b4y4-52202517361231.jpg)
![Immunohistochemistry: Enolase 2/Neuron-specific Enolase Antibody (9B4Y4) [Enolase 2/Neuron-specific Enolase] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Enolase 2/Neuron-specific Enolase Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15659_rabbit-enolase-2-neuron-specific-enolase-mab-9b4y4-52202517333854.jpg)
![Immunohistochemistry: Enolase 2/Neuron-specific Enolase Antibody (9B4Y4) [Enolase 2/Neuron-specific Enolase] - Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using Enolase 2/Neuron-specific Enolase Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15659_rabbit-enolase-2-neuron-specific-enolase-mab-9b4y4-52202517361234.jpg)
![Chromatin Immunoprecipitation: SOX2 Antibody (PO00-28) [NBP3-32972] - Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either SOX2 (NBP3-32972) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.](http://images.novusbio.com/fullsize/nbp3-32972_rabbit-sox2-mab-po00-28-26820241525104.jpg "Chromatin Immunoprecipitation: SOX2 Antibody (PO00-28) [NBP3-32972] - Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either SOX2 (NBP3-32972) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.")
![Western Blot: SOX2 Antibody (PO00-28) [NBP3-32972] - Western blot analysis of SOX2 on different lysates with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/2,000 dilution. Lane 1: NCCIT cell lysate Lane 2: F9 cell lysate Lane 3: HeLa cell lysate (negative) Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32972) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32972_rabbit-sox2-mab-po00-28-28820241249344.jpg)
![Immunohistochemistry: SOX2 Antibody (PO00-28) [NBP3-32972] - Immunohistochemical analysis of paraffin-embedded human trachea tissue with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32972) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32972_rabbit-sox2-mab-po00-28-298202414161844.jpg)
![Immunocytochemistry/ Immunofluorescence: SOX2 Antibody (PO00-28) [NBP3-32972] - Immunocytochemistry analysis of F9 cells labeling SOX2 with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32972_rabbit-sox2-mab-po00-28-29202410334048.jpg)