| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74344 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05210-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41262-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12895-0.025mg | |||
![Immunohistochemistry-Paraffin: Capicua Antibody [NBP2-33420] - Staining in human testis and liver tissues . Corresponding CIC RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Capicua-Antibody-Immunohistochemistry-Paraffin-NBP2-33420-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Capicua Antibody [NBP2-33420] - Staining in human testis and liver tissues . Corresponding CIC RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: Capicua Antibody [NBP2-33420] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-CIC antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/Capicua-Antibody-Western-Blot-NBP2-33420-img0013.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Capicua Antibody [NBP2-33420] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented](http://images.novusbio.com/fullsize/Capicua-Antibody-Western-Blot-NBP2-33420-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Capicua Antibody [NBP2-33420] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Staining is shown in green.](http://images.novusbio.com/fullsize/Capicua-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33420-img0012.jpg)
![Western Blot: Capicua Antibody [NBP2-33420] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line A-549](http://images.novusbio.com/fullsize/Capicua-Antibody-Western-Blot-NBP2-33420-img0005.jpg)
![Immunohistochemistry-Paraffin: Capicua Antibody [NBP2-33420] - Staining of human cerebellum shows strong nuclear immunoreactivity in Purkinje cells, as well as positivity in neurons in granular and molecular layers.](http://images.novusbio.com/fullsize/Capicua-Antibody-Immunohistochemistry-Paraffin-NBP2-33420-img0007.jpg)
![Immunohistochemistry-Paraffin: Capicua Antibody [NBP2-33420] - Staining of human endometrium shows moderate to strong nuclear positivity in glandular stromal cells.](http://images.novusbio.com/fullsize/Capicua-Antibody-Immunohistochemistry-Paraffin-NBP2-33420-img0008.jpg)
![Immunohistochemistry-Paraffin: Capicua Antibody [NBP2-33420] - Staining of human liver shows very weak nuclear positivity in hepatocytes.](http://images.novusbio.com/fullsize/Capicua-Antibody-Immunohistochemistry-Paraffin-NBP2-33420-img0009.jpg)
![Immunohistochemistry-Paraffin: Capicua Antibody [NBP2-33420] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Capicua-Antibody-Immunohistochemistry-Paraffin-NBP2-33420-img0010.jpg)
![Western Blot: SPR Antibody (4F5) [NBP2-03256] Analysis of extracts (35ug) from 9 different cell lines by using anti-SPR monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Western-Blot-NBP2-03256-img0013.jpg "Western Blot: SPR Antibody (4F5) [NBP2-03256] Analysis of extracts (35ug) from 9 different cell lines by using anti-SPR monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: SPR Antibody (4F5) [NBP2-03256] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SPR.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunocytochemistry-Immunofluorescence-NBP2-03256-img0012.jpg)
![Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Human pancreas tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunohistochemistry-Paraffin-NBP2-03256-img0011.jpg)
![Flow Cytometry: SPR Antibody (4F5) [NBP2-03256] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SPR antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Flow-Cytometry-NBP2-03256-img0002.jpg)
![Western Blot: SPR Antibody (4F5) [NBP2-03256] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPR (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPR.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Western-Blot-NBP2-03256-img0003.jpg)
![Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunohistochemistry-Paraffin-NBP2-03256-img0004.jpg)
![Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunohistochemistry-Paraffin-NBP2-03256-img0005.jpg)
![Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunohistochemistry-Paraffin-NBP2-03256-img0006.jpg)
![Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunohistochemistry-Paraffin-NBP2-03256-img0007.jpg)
![Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunohistochemistry-Paraffin-NBP2-03256-img0008.jpg)
![Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Human Kidney tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunohistochemistry-Paraffin-NBP2-03256-img0009.jpg)
![Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Human liver tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunohistochemistry-Paraffin-NBP2-03256-img0010.jpg)
![Flow Cytometry: SPR Antibody (4F5) [NBP2-03256] - Analysis of Jurkat cells, using anti-SPR antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Flow-Cytometry-NBP2-03256-img0001.jpg)
![Western Blot: NPY1R Antibody [NBP1-59008] - Human Fetal Brain Lysate, concentration 1ug/ml.](http://images.novusbio.com/fullsize/NPY1R-Antibody-Western-Blot-NBP1-59008-img0010.jpg "Western Blot: NPY1R Antibody [NBP1-59008] - Human Fetal Brain Lysate, concentration 1ug/ml.")
![Immunohistochemistry: NPY1R Antibody [NBP1-59008] - Analysis of mouse gut tissue.](http://images.novusbio.com/fullsize/NPY1R-Antibody-Immunohistochemistry-NBP1-59008-img0011.jpg)
![Immunohistochemistry-Paraffin: NPY1R Antibody [NBP1-59008] - Human liver.](http://images.novusbio.com/fullsize/NPY1R-Antibody-Immunohistochemistry-Paraffin-NBP1-59008-img0007.jpg)
![Immunohistochemistry-Paraffin: NPY1R Antibody [NBP1-59008] - Human Testis Tissue, 5ug/ml](http://images.novusbio.com/fullsize/NPY1R-Antibody-Immunohistochemistry-Paraffin-NBP1-59008-img0008.jpg)
![Immunohistochemistry-Paraffin: NPY1R Antibody [NBP1-59008] - Human Gut Tissue, primary dilution 10ug/ml and 2ug/ml. Secondary goat anti-rabbit Cy3 at 1:500.](http://images.novusbio.com/fullsize/NPY1R-Antibody-Immunohistochemistry-Paraffin-NBP1-59008-img0009.jpg)
![Western Blot: NPY1R Antibody [NBP1-59008] - Representative immunoblotting images showing the protein expression of NPY1R in (E) MCF7, (F) T47D and (G) ZR75-1 parental cells and their EDR and TamR derivatives. Ponceau staining was used to assess equal protein loading for visual assessment. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35121782/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp1-59008_rabbit-polyclonal-npy1r-antibody-25520231539514.jpg)
![Western Blot: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPR (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPR.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Western-Blot-NBP2-74344-img0002.jpg "Western Blot: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPR (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPR.")
![Immunocytochemistry/Immunofluorescence: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SPR.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-74344-img0005.jpg)
![Immunohistochemistry: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Staining of paraffin-embedded Human liver tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74344-img0012.jpg)
![Flow Cytometry: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-SPR antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-74344-img0004.jpg)
![Western Blot: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Analysis of extracts (35ug) from 9 different cell lines by using anti-SPR monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Western-Blot-NBP2-74344-img0001.jpg)
![Immunohistochemistry: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74344-img0006.jpg)
![Immunohistochemistry: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74344-img0007.jpg)
![Immunohistochemistry: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74344-img0008.jpg)
![Immunohistochemistry: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74344-img0009.jpg)
![Immunohistochemistry: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74344-img0010.jpg)
![Immunohistochemistry: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Staining of paraffin-embedded Human Kidney tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74344-img0011.jpg)
![Immunohistochemistry: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Staining of paraffin-embedded Human pancreas tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74344-img0013.jpg)
![Flow Cytometry: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Analysis of Jurkat cells, using anti-SPR antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-74344-img0003.jpg)
![Western Blot: NF-H Antibody (9B12) [NBP1-05210] - Analysis of different tissue lysates using mouse mAb to NF-H, dilution 1:10000 (Green): [1] protein standard, [2] rat spinal cord [3] mouse spinal cord, and [4] cow spinal cord. Strong band at about 200-220kDa corresponds to the major phosphorylated form of the NF-H subunit. Smaller proteolytic fragments of NF-H are also detected in some preparations.](http://images.novusbio.com/fullsize/NF-H-Antibody-9B12-Western-Blot-NBP1-05210-img0006.jpg "Western Blot: NF-H Antibody (9B12) [NBP1-05210] - Analysis of different tissue lysates using mouse mAb to NF-H, dilution 1:10000 (Green): [1] protein standard, [2] rat spinal cord [3] mouse spinal cord, and [4] cow spinal cord. Strong band at about 200-220kDa corresponds to the major phosphorylated form of the NF-H subunit. Smaller proteolytic fragments of NF-H are also detected in some preparations.")
![Immunocytochemistry/Immunofluorescence: NF-H Antibody (9B12) [NBP1-05210] - Mixed neuron/glial cultures stained with NBP1-05210 (red) and rabbit GFAP antibody NB300-141 (green). Axonal profiles are stained in red, while astrocytic cells are revealed in green. Nuclei are revealed with a fluorescent DNA stain (blue).](http://images.novusbio.com/fullsize/NF-H-Antibody-9B12-Immunocytochemistry-Immunofluorescence-NBP1-05210-img0003.jpg)
![Immunohistochemistry Free-Floating: NF-H Antibody (9B12) [NBP1-05210] - Analysis of a rat brain coronal section of the third ventricle stained with mouse monoclonal antibody to phosphorylated NF-H, dilution 1:5,000 (Green). Hoechst staining of nuclear DNA (Blue). Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24hrs, cut to 45uM, and free-floating sections were stained with above antibody. The antibody is a robust marker of the axons of neuronal cells.](http://images.novusbio.com/fullsize/NF-H-Antibody-9B12-Immunohistochemistry-Free-Floating-NBP1-05210-img0005.jpg)
![Wallerian degeneration and mitochondrial degeneration in axon for rat’s distal nerve stump. a, Representative images of toluidine blue staining, immunohistochemistry (NF-H), electron microscopy, before (pre) and 1, 2, and 14 d after axotomy. Immunohistochemistry was obtained the same results for three individuals. Scale bar, * 10 μm, ** 2 μm. Arrows, myelinated axon; arrowheads, demyelinated Schwann cell. b, Quantification of the myelinated axon. c, d, Quantification of the G-ratio [c: cumulative G-ratio per individual, d: scatter plot, Spearman’s rank correlation coefficient and p values showing G-ratio of individual myelinated axons against axon diameter (n = 400)]. The histologic WD was observed significantly from 2 d after axotomy. In axons, before (pre) and 1 and 2 d after axotomy were evaluated, except for after 14 d with completely collapsed axon morphology (n = 4 rat per group). e, Representative images of mitochondrial findings, before (pre) and 1, 2, and 14 d after axotomy. Evaluation was performed in axons and Schwann cells separately. Scale bar, * 200 nm. f, Violin plot of mitochondrial diameter (nm; n = 200). Cumulative mitochondria diameter per individual are also shown (n = 4 rat per group). g, Quantitative analysis of atypical mitochondria (%; n = 4 rat per group). In axons, mitochondria were significantly degenerated from 1 d after axotomy, whereas in Schwann cells, mitochondria were not significantly degenerated until 14 d after axotomy. All histologic evaluations were performed 3 mm distal to the sectional end and corresponding uninjured nerve. Error bars indicate SD; *p < 0.05, ***p < 0.001, one-way ANOVA followed by the Tukey’s post hoc test (for comparison of axons and Schwann cells, respectively). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36894321), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-05210_mouse-monoclonal-nf-h-antibody-9b12-immunocytochemistry-immunofluorescence-132202617595724.jpg)
![Western Blot: Ndufs4 Antibody [NBP1-31465] - Sample(30ug whole cell lysate) A: 293T 12% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Ndufs4-Antibody-Western-Blot-NBP1-31465-img0007.jpg "Western Blot: Ndufs4 Antibody [NBP1-31465] - Sample(30ug whole cell lysate) A: 293T 12% SDS PAGE, antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Ndufs4 Antibody [NBP1-31465] - Analysis of methanol-fixed HeLa, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Ndufs4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31465-img0003.jpg)
![Immunohistochemistry-Paraffin: Ndufs4 Antibody [NBP1-31465] - Human ovarian cancer, using NDUFS4 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Ndufs4-Antibody-Immunohistochemistry-Paraffin-NBP1-31465-img0004.jpg)
![Western Blot: Ndufs4 Antibody [NBP1-31465] - Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Ndufs4-Antibody-Western-Blot-NBP1-31465-img0005.jpg)
![Western Blot: Ndufs4 Antibody [NBP1-31465] - 50 ug Rat heart lysate/extract 12% SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/Ndufs4-Antibody-Western-Blot-NBP1-31465-img0006.jpg)
![Immunohistochemistry-Paraffin: TRPV4 Antibody [NB110-74960] - Rat kidney using Rabbit antibody to TRPV4, dilution 1: 1000, incubated 30 min at RT (using Autostainer).](http://images.novusbio.com/fullsize/TRPV4-Antibody-Immunohistochemistry-Paraffin-NB110-74960-img0005.jpg "Immunohistochemistry-Paraffin: TRPV4 Antibody [NB110-74960] - Rat kidney using Rabbit antibody to TRPV4, dilution 1: 1000, incubated 30 min at RT (using Autostainer).")
![Immunohistochemistry-Paraffin: TRPV4 Antibody [NB110-74960] - Rat kidney using Rabbit antibody to TRPV4, dilution 1: 1000, incubated 30 min at RT (using Autostainer)](http://images.novusbio.com/fullsize/TRPV4-Antibody-Immunohistochemistry-Paraffin-NB110-74960-img0003.jpg)
![Western Blot: TRPV4 Antibody [NBP2-41262] - Analysis of TRPV4 in (A) human testis tissue and (B) SK-N-SH cell lysate with TRPV4 antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/TRPV4-Antibody-Western-Blot-NBP2-41262-img0005.jpg "Western Blot: TRPV4 Antibody [NBP2-41262] - Analysis of TRPV4 in (A) human testis tissue and (B) SK-N-SH cell lysate with TRPV4 antibody at 1 ug/mL.")
![Immunohistochemistry: TRPV4 Antibody - BSA Free [NBP2-41262] - Immunohistochemistry of TRPV4 in human lung tissue with TRPV4 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41262_rabbit-polyclonal-trpv4-antibody-2711202419553050.jpg)
![Immunohistochemistry: TRPV4 Antibody - BSA Free [NBP2-41262] - Immunohistochemistry of TRPV4 in human kidney tissue with TRPV4 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41262_rabbit-polyclonal-trpv4-antibody-2711202419553036.gif)
![Immunocytochemistry/ Immunofluorescence: TRPV4 Antibody - BSA Free [NBP2-41262] - Immunofluorescence of TRPV4 in human lung tissue with TRPV4 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41262_rabbit-polyclonal-trpv4-antibody-271120242004123.jpg)
![Immunocytochemistry/ Immunofluorescence: TRPV4 Antibody - BSA Free [NBP2-41262] - Immunofluorescence of TRPV4 in human kidney tissue with TRPV4 antibody at 5 ug/ml.Red: TRPV4 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp2-41262_rabbit-polyclonal-trpv4-antibody-271120242004135.gif)
![Western Blot: HCN2 Antibody (S71-37) [NBP2-12895] - Western Blot analysis of Mouse Brain showing detection of ~95 kDa HCN2 protein using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71 (NBP2-12895). Lane 1: MW Ladder. Lane 2: Mouse Brain (15 ug). Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (NBP2-12895) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~95 kDa.](http://images.novusbio.com/fullsize/HCN2-Antibody-S71-37-Western-Blot-NBP2-12895-img0010.jpg "Western Blot: HCN2 Antibody (S71-37) [NBP2-12895] - Western Blot analysis of Mouse Brain showing detection of ~95 kDa HCN2 protein using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71 (NBP2-12895). Lane 1: MW Ladder. Lane 2: Mouse Brain (15 ug). Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (NBP2-12895) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~95 kDa.")
![Immunocytochemistry/Immunofluorescence: HCN2 Antibody (S71-37) [NBP2-12895] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71 (NBP2-12895). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (NBP2-12895) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) HCN2 Antibody (D) Composite.](http://images.novusbio.com/fullsize/HCN2-Antibody-S71-37-Immunocytochemistry-Immunofluorescence-NBP2-12895-img0013.jpg)
![Immunohistochemistry: HCN2 Antibody (S71-37) [NBP2-12895] - Immunohistochemistry analysis using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71 (NBP2-12895). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (NBP2-12895) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/HCN2-Antibody-S71-37-Immunohistochemistry-NBP2-12895-img0012.jpg)
![Western Blot: HCN2 Antibody (S71-37) [NBP2-12895] - Western Blot analysis of Rat brain membrane lysate showing detection of HCN2 protein using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71 (NBP2-12895). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (NBP2-12895) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HCN2-Antibody-S71-37-Western-Blot-NBP2-12895-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: HCN2 Antibody (S71-37) [NBP2-12895] - Human hippocampal tissues. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.](http://images.novusbio.com/fullsize/HCN2-Antibody-S71-37-Immunocytochemistry-Immunofluorescence-NBP2-12895-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: HCN2 Antibody (S71-37) [NBP2-12895] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71-37 (NBP2-12895). Tissue: Differentiated SH-SY5Y. Species: Human. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (NBP2-12895) at 1:100. Secondary Antibody: AlexaFluor 488. Counterstain: phalloidin (Alexa 647, red), beta tubulin (Anti-beta III Tubulin Ab, Alexa 555, magenta) Hoechst (blue). (A) Phalloidin (B) Anti-beta III Tubulin Ab. (C) HCN2 Antibody. (D) Hoechst (E) Composite.](http://images.novusbio.com/fullsize/HCN2-Antibody-S71-37-Immunocytochemistry-Immunofluorescence-NBP2-12895-img0014.jpg)
![Immunohistochemistry: HCN2 Antibody (S71-37) [NBP2-12895] - Immunohistochemistry analysis using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71 (NBP2-12895). Tissue: frozen brain section. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (NBP2-12895) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT.](http://images.novusbio.com/fullsize/HCN2-Antibody-S71-37-Immunohistochemistry-NBP2-12895-img0011.jpg)
![Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP2-57836] - Analysis in human adrenal gland and testis tissues. Corresponding Dopamine beta-Hydroxylase RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Dopamine-beta-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-57836-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP2-57836] - Analysis in human adrenal gland and testis tissues. Corresponding Dopamine beta-Hydroxylase RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP2-57836] - Staining of human adrenal gland, kidney, lower gastrointestinal and testis using Anti-Dopamine beta-Hydroxylase antibody NBP2-57836 (A) shows similar protein distribution across tissues to independent antibody NBP2-55677 (B).](http://images.novusbio.com/fullsize/Dopamine-beta-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-57836-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: Dopamine beta-Hydroxylase Antibody [NBP2-57836] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Dopamine-beta-Hydroxylase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57836-img0007.jpg)
![Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP2-57836] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/Dopamine-beta-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-57836-img0018.jpg)
![Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP2-57836] - Staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Dopamine-beta-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-57836-img0013.jpg)
![Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP2-57836] - Staining of human colon shows moderate cytoplasmic positivity in peripheral ganglion.](http://images.novusbio.com/fullsize/Dopamine-beta-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-57836-img0015.jpg)
![Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP2-57836] - Staining of human kidney shows very weak positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/Dopamine-beta-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-57836-img0017.jpg)
![Western Blot: Dopamine beta-Hydroxylase Antibody [NBP2-57836] - Analysis in human cell line SH-SY5Y.](http://images.novusbio.com/fullsize/antibody/nbp2-57836_rabbit-polyclonal-dopamine-beta-hydroxylase-antibody-western-blot-2120249955..jpg)
![Western Blot: Ankyrin Brain Antibody [NBP2-33863] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Western-Blot-NBP2-33863-img0003.jpg "Western Blot: Ankyrin Brain Antibody [NBP2-33863] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue")
![Immunocytochemistry/Immunofluorescence: Ankyrin Brain Antibody [NBP2-33863] - Staining of human cell line RH-30 shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33863-img0008.jpg)
![Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Staining of human placenta shows no positivity in trophoblastic cells as expected.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunohistochemistry-Paraffin-NBP2-33863-img0012.jpg)
![Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunohistochemistry-Paraffin-NBP2-33863-img0004.jpg)
![Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunohistochemistry-Paraffin-NBP2-33863-img0005.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Analysis in human cerebral cortex and skin tissues using NBP2-33863 antibody. Corresponding ANK2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunohistochemistry-Paraffin-NBP2-33863-img0009.jpg)
![Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Staining of human cerebral cortex shows strong positivity in neuropil.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunohistochemistry-Paraffin-NBP2-33863-img0010.jpg)
![Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Staining of human kidney shows weak to moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunohistochemistry-Paraffin-NBP2-33863-img0011.jpg)
![Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Staining of human skin shows no positivity in squamous epithelial cells as expected.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunohistochemistry-Paraffin-NBP2-33863-img0013.jpg)