| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89152-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43954 | |||
| BSA Free | NBP2-34495-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81592-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90125-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38622-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61780-0.025ml | |||
![Western Blot: BIRC6 Antibody [NB110-40730] - Dual IAP-targeting ASOs knockdown BIRC6, cIAP1 or survivin proteins and lead to marked suppression of CRPC cell proliferation. Western blotting showing protein levels of BIRC6, cIAP1 and survivin in two CRPC cell lines (A) PC-3 cells and (B) C4-2 cells transfected with Mock or increasing dosages of scrambled ASO (Scrb), dASOs 6w2 and 6w5 for 72 hr. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/2229), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BIRC6-Antibody-Western-Blot-NB110-40730-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: BIRC6 Antibody [NB110-40730] - Dual IAP-targeting ASOs knockdown BIRC6, cIAP1 or survivin proteins and lead to marked suppression of CRPC cell proliferation. Western blotting showing protein levels of BIRC6, cIAP1 and survivin in two CRPC cell lines (A) PC-3 cells and (B) C4-2 cells transfected with Mock or increasing dosages of scrambled ASO (Scrb), dASOs 6w2 and 6w5 for 72 hr. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/2229), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: BIRC6 Antibody [NB110-40730] - Treatments with dASOs resulted in significant lower viable tumor volume. Representative images of control ASO-6w2- and 6w5-treated PC-3 xenografts using H&E, and IHC staining of BIRC6, cleaved-caspase 3 and Ki-67. 20x magnification. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/2229), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BIRC6-Antibody-Immunohistochemistry-NB110-40730-img0005.jpg)
![Immunohistochemistry-Paraffin: BIRC6 Antibody [NB110-40730] - FFPE section of human metastatic lymph node. Antibody: Affinity purified rabbitanti-BIRC6/Apollon used at a dilution of 1:250. Detection: DAB](http://images.novusbio.com/fullsize/BIRC6-Antibody-Immunohistochemistry-Paraffin-NB110-40730-img0003.jpg)
![Immunohistochemistry-Paraffin: BIRC6 Antibody [NB110-40730] - FFPE section of human ovarian tumor. Antibody: Affinity purified rabbit antiBIRC6/Apollon used at a dilution of 1:250. Detection: DAB](http://images.novusbio.com/fullsize/BIRC6-Antibody-Immunohistochemistry-Paraffin-NB110-40730-img0002.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry: BIRC6 Antibody [NB110-40730] - Elevated BIRC6 expression is associated with advanced stages of prostate cancer: co-upregulation of other IAP members. Representative images of correlated expressions between BIRC6 and survivin, XIAP and cIAP1. 20x magnification, scale bar, 100 um. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/2229), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BIRC6-Antibody-Immunohistochemistry-NB110-40730-img0004.jpg)
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP1-89152] - Analysis in human cell lines A-549 and A-431 using anti-ALDH1A1 antibody. Corresponding ALDH1A1 RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-Western-Blot-NBP1-89152-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP1-89152] - Analysis in human cell lines A-549 and A-431 using anti-ALDH1A1 antibody. Corresponding ALDH1A1 RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP1-89152] - Staining of human cell line A549 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89152-img0006.jpg)
![Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP1-89152] - Staining of human tonsil shows very weak positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-Immunohistochemistry-Paraffin-NBP1-89152-img0015.jpg)
![Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP1-89152] - Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-Immunohistochemistry-Paraffin-NBP1-89152-img0012.jpg)
![Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP1-89152] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-Immunohistochemistry-Paraffin-NBP1-89152-img0013.jpg)
![Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP1-89152] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-Immunohistochemistry-Paraffin-NBP1-89152-img0014.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining in human skin and skeletal muscle tissues. Corresponding p53 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunohistochemistry-Paraffin-NBP2-34495-img0021.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining in human skin and skeletal muscle tissues. Corresponding p53 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: p53 Antibody (CL2199) [NBP2-34495] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-P53 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-PPIB monoclonal antibody was used as loading control.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Western-Blot-NBP2-34495-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495] - Staining in U2OS cell line with Anti-p53 monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunocytochemistry-Immunofluorescence-NBP2-34495-img0014.jpg)
![Western Blot: p53 Antibody (CL2199) [NBP2-34495] - Lane 1: Marker [kDa] Lane 2: Human cell line U-251](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Western-Blot-NBP2-34495-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495] - Staining of human cell line A431 showing cell cycle dependent nuclear (without nucleoli)Staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunocytochemistry-Immunofluorescence-NBP2-34495-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495] - Staining in MCF7 cell line with Anti-p53 monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunocytochemistry-Immunofluorescence-NBP2-34495-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495] - Staining in U251 cell line with Anti-p53 monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunocytochemistry-Immunofluorescence-NBP2-34495-img0013.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining of human colorectal cancer shows strong nuclear positivity in tumor cells, but not in normal mucosa.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunohistochemistry-Paraffin-NBP2-34495-img0017.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining of human lung cancer (squamous cell carcinoma) shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunohistochemistry-Paraffin-NBP2-34495-img0018.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining of human skin shows moderate to strong nuclear positivity in a subset of epidermal cells.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunohistochemistry-Paraffin-NBP2-34495-img0019.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunohistochemistry-Paraffin-NBP2-34495-img0020.jpg)
![Simple Western: p53 Antibody (CL2199) [NBP2-34495] - Simple Western lane view shows a specific band for P53 in 0.2 mg/ml of Hek293T lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Simple-Western-NBP2-34495-img0009.jpg)
![Simple Western: p53 Antibody (CL2199) [NBP2-34495] - Electropherogram image(s) of corresponding Simple Western lane view. p53 antibody was used at 1:50 dilution on Hek293T lysate(s).](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Simple-Western-NBP2-34495-img0011.jpg)
![Western Blot: HIP1 Antibody [NBP1-81592] - Analysis in human cell lines A-549 and Caco-2 using Anti-HIP1 antibody. Corresponding HIP1 RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/HIP1-Antibody-Western-Blot-NBP1-81592-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: HIP1 Antibody [NBP1-81592] - Analysis in human cell lines A-549 and Caco-2 using Anti-HIP1 antibody. Corresponding HIP1 RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: HIP1 Antibody [NBP1-81592] - Staining of human cell line U-251 MG shows localization to vesicles.](http://images.novusbio.com/fullsize/HIP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81592-img0018.jpg)
![Immunohistochemistry-Paraffin: HIP1 Antibody [NBP1-81592] - Staining of human testis using Anti-HIP1 antibody NBP1-81592.](http://images.novusbio.com/fullsize/HIP1-Antibody-Immunohistochemistry-Paraffin-NBP1-81592-img0023.jpg)
![Western Blot: HIP1 Antibody [NBP1-81592] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/HIP1-Antibody-Western-Blot-NBP1-81592-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HIP1 Antibody [NBP1-81592] - Analysis in human testis and liver tissues using Anti-HIP1 antibody. Corresponding HIP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HIP1-Antibody-Immunohistochemistry-Paraffin-NBP1-81592-img0014.jpg)
![Immunohistochemistry-Paraffin: HIP1 Antibody [NBP1-81592] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/HIP1-Antibody-Immunohistochemistry-Paraffin-NBP1-81592-img0016.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HIP1 Antibody [NBP1-81592] - Staining in human testis and liver tissues using anti-HIP1 antibody. Corresponding HIP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HIP1-Antibody-Immunohistochemistry-Paraffin-NBP1-81592-img0017.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: HIP1 Antibody [NBP1-81592] - Staining of human colon, kidney, liver and testis using Anti-HIP1 antibody NBP1-81592 (A) shows similar protein distribution across tissues to independent antibody NBP1-81593 (B).](http://images.novusbio.com/fullsize/HIP1-Antibody-Immunohistochemistry-Paraffin-NBP1-81592-img0019.jpg)
![Immunohistochemistry-Paraffin: HIP1 Antibody [NBP1-81592] - Staining of human colon.](http://images.novusbio.com/fullsize/HIP1-Antibody-Immunohistochemistry-Paraffin-NBP1-81592-img0020.jpg)
![Immunohistochemistry-Paraffin: HIP1 Antibody [NBP1-81592] - Staining of human kidney.](http://images.novusbio.com/fullsize/HIP1-Antibody-Immunohistochemistry-Paraffin-NBP1-81592-img0021.jpg)
![Immunohistochemistry-Paraffin: HIP1 Antibody [NBP1-81592] - Staining of human liver using Anti-HIP1 antibody NBP1-81592.](http://images.novusbio.com/fullsize/HIP1-Antibody-Immunohistochemistry-Paraffin-NBP1-81592-img0022.jpg)
![Immunohistochemistry-Paraffin: NGF R/TNFRSF16/p75NTR Antibody [NBP2-19669] - Rat brain. p75 NGF Receptor antibody diluted at 1:500.](http://images.novusbio.com/fullsize/NGF-R-TNFRSF16-p75NTR-Antibody-Immunohistochemistry-Paraffin-NBP2-19669-img0005.jpg "Immunohistochemistry-Paraffin: NGF R/TNFRSF16/p75NTR Antibody [NBP2-19669] - Rat brain. p75 NGF Receptor antibody diluted at 1:500.")
![Western Blot: NGFR/TNFRSF16/p75NTR Antibody [NBP2-19669] - Untreated (-) and treated (+) PC-3 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NGFR/TNFRSF16/p75NTR antibody (NBP2-19669) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19669_rabbit-polyclonal-ngfr-tnfrsf16-p75ntr-antibody-91020242063324.jpg)
![p75 NGF Receptor / CD271 antibody detects p75 NGF Receptor / CD271 protein by immunofluorescent analysis.Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: p75 NGF Receptor / CD271 stained by p75 NGF Receptor / CD271 antibody (NBP2-19669) diluted at 1:500.Red: Tau, stained by Tau antibody [GT287] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-19669_rabbit-polyclonal-ngfr-tnfrsf16-p75ntr-antibody-immunocytochemistry-immunofluorescence-261120251823614.jpg)
![p75 NGF Receptor antibody detects p75 NGF Receptor protein expression by immunohistochemical analysis.Sample: Frozen sectioned E13.5 Rat brain. Green: p75 NGF Receptor protein stained by p75 NGF Receptor antibody (NBP2-19669) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-19669_rabbit-polyclonal-ngfr-tnfrsf16-p75ntr-antibody-immunohistochemistry-frozen-2611202518303814.jpg)
![Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - TNF Induces phosphorylation of p65 in KBM-5 cells. Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS276 p65. Pan reactive anti p65 was used a control to show the presence of total p65 in both the cytoplasmic and nuclear extracts. Phosphorylation of p65 occurs after approximately 10 min of TNF exposure. Migration of phosphorylated p65 into the nucleus occurs within a similar time frame. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemi -luminescent detection method.](http://images.novusbio.com/fullsize/RelA-NFkB p65 [p Ser276] Antibody-Western Blot-NBP1-77807-img0010.jpg "Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - TNF Induces phosphorylation of p65 in KBM-5 cells. Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS276 p65. Pan reactive anti p65 was used a control to show the presence of total p65 in both the cytoplasmic and nuclear extracts. Phosphorylation of p65 occurs after approximately 10 min of TNF exposure. Migration of phosphorylated p65 into the nucleus occurs within a similar time frame. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemi -luminescent detection method.")
![Immunohistochemistry: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - Diluted to1:500 to detect p65 in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Immunohistochemistry-NBP1-77807-img0007.jpg)
![Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - pS529 shows phospho p65 staining in carcinoma cells.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Western-Blot-NBP1-77807-img0005.jpg)
![Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - pS276 shows phospho p65 staining in carcinoma cells. Western blot of total protein lysates from various human head and neck tumors shows phospho p65 staining in tumor cell lines using phospho specific polyclonal anti-human pS276 p65. Lanes 1-6 contain protein lysates from human squamal carcinoma cell lines. Lane 7 is a protein lysate from a primary culture of human keratinocytes and does not show significant levels of phosphorylated p65. Lane 8 contains protein lysate from ATCC SCC9 cells (also a head and neck squamal carcinoma). Lane 9 contains lysate from EGF-induced human derived A431 cells. Lane 10 (not shown) contains a molecular weight standard. Concurrent staining with anti-beta microtubulin (not shown) was used to confirm equal protein loading in all lanes. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemiluminescent detection method.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Western-Blot-NBP1-77807-img0006.jpg)
![Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - TNF Induces phosphorylation of p65 in KBM-5 cells.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Western-Blot-NBP1-77807-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Caspase-3 Antibody [NBP1-90125] - Staining of human cell line U-251 MG shows localization to nucleoplasm and mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90125-img0015.jpg "Immunocytochemistry/Immunofluorescence: Caspase-3 Antibody [NBP1-90125] - Staining of human cell line U-251 MG shows localization to nucleoplasm and mitochondria. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Analysis in human parathyroid gland and skeletal muscle tissues using NBP2-38622 antibody. Corresponding CASR RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Analysis in human parathyroid gland and skeletal muscle tissues using NBP2-38622 antibody. Corresponding CASR RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human kidney, liver, pancreas and parathyroid gland using Anti-CASR antibody NBP2-38622 (A) shows similar protein distribution across tissues to independent antibody NBP1-84687 (B).](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0012.jpg)
![Western Blot: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Analysis in control (vector only transfected HEK293T lysate) and CASR over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Western-Blot-NBP2-38622-img0006.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0017.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human parathyroid gland shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0014.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human kidney shows moderate membranous positivity in cells in distal tubules.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0015.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human pancreas shows weak to moderate membranous positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0016.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0018.jpg)
![Western Blot: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis using YWHAB mAb against HEK293 (1) and YWHAB (AA: 1-246)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-Western-Blot-NBP2-61780-img0009.jpg "Western Blot: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis using YWHAB mAb against HEK293 (1) and YWHAB (AA: 1-246)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis of HepG2 cells using YWHAB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-Immunocytochemistry-Immunofluorescence-NBP2-61780-img0005.jpg)
![Immunohistochemistry: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis of paraffin-embedded endometrial cancer tissues using YWHAB mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-Immunohistochemistry-NBP2-61780-img0007.jpg)
![Western Blot: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis using YWHAB mAb against human YWHAB (AA: 1-246) recombinant protein. (Expected MW is 54 kDa)](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-Western-Blot-NBP2-61780-img0008.jpg)
![Western Blot: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis using YWHAB mouse mAb against Hela (1), NIH/3T3 (2), C6 (3), A431 (4), K562 (5), PC-12 (6), and U937 (7) cell lysate.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-Western-Blot-NBP2-61780-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis of GC-7901 cells using YWHAB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-Immunocytochemistry-Immunofluorescence-NBP2-61780-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis of Hela cells using YWHAB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-Immunocytochemistry-Immunofluorescence-NBP2-61780-img0004.jpg)
![Immunohistochemistry: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis of paraffin-embedded rectum cancer tissues using YWHAB mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-Immunohistochemistry-NBP2-61780-img0006.jpg)
![ELISA: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-ELISA-NBP2-61780-img0001.jpg)
![ELISA: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis of Hela cells using YWHAB mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-ELISA-NBP2-61780-img0002.jpg)
![Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [HL2915] (NBP3-48751) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48751_rabbit-akt-mab-hl2915-western-blot-1411202518341523.jpg "Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [HL2915] (NBP3-48751) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![AKT antibody [HL2915] detects AKT protein by immunohistochemical analysis.Sample: Paraffin-embedded rat esophagus.AKT stained by AKT antibody [HL2915] (NBP3-48751) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48751_rabbit-akt-mab-hl2915-immunohistochemistry-paraffin-1411202518382820.jpg)
![Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [HL2915] (NBP3-48751) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48751_rabbit-akt-mab-hl2915-western-blot-1411202518435619.jpg)
![AKT antibody [HL2915] detects AKT protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.AKT stained by AKT antibody [HL2915] (NBP3-48751) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48751_rabbit-akt-mab-hl2915-immunohistochemistry-paraffin-141120251841520.jpg)
![AKT antibody [HL2915] detects AKT protein by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: AKT stained by AKT antibody [HL2915] (NBP3-48751) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-48751_rabbit-akt-mab-hl2915-immunocytochemistry-immunofluorescence-1411202518424111.jpg)
![AKT antibody [HL2915] detects AKT protein by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.AKT stained by AKT antibody [HL2915] (NBP3-48751) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48751_rabbit-akt-mab-hl2915-immunohistochemistry-paraffin-1411202518312110.jpg)
![Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [HL2915] (NBP3-48751) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48751_rabbit-akt-mab-hl2915-western-blot-1411202518394118.jpg)
![Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [HL2915] (NBP3-48751) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48751_rabbit-akt-mab-hl2915-western-blot-1411202518334523.jpg)
![Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - TNF Induces phosphorylation of p65 in KBM-5 cells. Nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS529 p65. Anti-beta-actin staining confirms loading of equivalent amounts of protein. HRP conjugated Gt-anti-Rabbit IgG used to develop blot using a chemiluminescent detection method. Other detection methods will yield similar results.](http://images.novusbio.com/fullsize/RelA-NFkB p65 [p Ser529] Antibody-Western Blot-NBP1-77808-img0005.jpg "Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - TNF Induces phosphorylation of p65 in KBM-5 cells. Nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS529 p65. Anti-beta-actin staining confirms loading of equivalent amounts of protein. HRP conjugated Gt-anti-Rabbit IgG used to develop blot using a chemiluminescent detection method. Other detection methods will yield similar results.")
![Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - pS529 shows phospho p65 staining in carcinoma cells. western blot of total protein lysates from various human head and neck tumors shows phospho p65 staining in tumor cell lines using phospho specific polyclonal anti-human pS529 p65. Lanes 1-6 contain protein lysates from human squamal carcinoma cell lines. Lane 7 is a protein lysate from a primary culture of human keratinocytes. Lane 8 contains protein lysate from ATCC SCC9 cells (also a head and neck squamal carcinoma). Lane 9 contains lysate from EGF-induced human derived A431 cells. Lane 10 (not shown) contains a molecular weight standard. Concurrent staining with anti-beta microtubulin (not shown) was used to confirm equal protein loading in all lanes. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemiluminescent detection method.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser529]-Antibody-Western-Blot-NBP1-77808-img0003.jpg)
![Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - TNF Induces phosphorylation of p65 in KBM-5 cells. Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS276 p65. Pan reactive anti p65 (NBP1-77808) was used a control to show the presence of total p65 in both the cytoplasmic and nuclear extracts. Phosphorylation of p65 occurs after approximately 10 min of TNF exposure. Migration of phosphorylated p65 into the nucleus occurs within a similar time frame. HRP conjugated Gt-anti-Rabbit IgG was used to develop the western blot using a chemi-luminescent detection method.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser529]-Antibody-Western-Blot-NBP1-77808-img0004.jpg)
![Western Blot: BIRC6 Antibody [NB300-264] - Detection of Human and Mouse BIRC6 by Western Blot. Samples: Whole cell lysate from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BIRC6 antibody NB300-264 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/BIRC6-Antibody-Western-Blot-NB300-264-img0012.jpg "<b>Biological Strategies Validation. </b>Western Blot: BIRC6 Antibody [NB300-264] - Detection of Human and Mouse BIRC6 by Western Blot. Samples: Whole cell lysate from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BIRC6 antibody NB300-264 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: BIRC6 Antibody [NB300-264] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-BIRC6/Apollon used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB Coutnerstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/BIRC6-Antibody-Immunohistochemistry-Paraffin-NB300-264-img0008.jpg)
![Immunoprecipitation: BIRC6 Antibody [NB300-264] - Detection of human BIRC6 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BIRC6 antibody NB300-264 (lot 2) used for IP at 6 ug per reaction. BIRC6 was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated BIRC6, NB300-264 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/BIRC6-Antibody-Immunoprecipitation-NB300-264-img0013.jpg)