| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-14045-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43897 | |||
| BSA Free | NBP2-52872-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-34131-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-62693-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82544-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59789-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38012-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54915-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61841-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61848-0.025ml | |||
![Immunocytochemistry/Immunofluorescence: GFR alpha-1/GDNF R alpha-1 Antibody [NBP2-14045] - Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.](http://images.novusbio.com/fullsize/GFR-alpha-1-GDNF-R-alpha-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-14045-img0003.jpg "Immunocytochemistry/Immunofluorescence: GFR alpha-1/GDNF R alpha-1 Antibody [NBP2-14045] - Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.")
![Immunohistochemistry-Paraffin: GFR alpha-1/GDNF R alpha-1 Antibody [NBP2-14045] - Staining of human kidney shows weak granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/GFR-alpha-1-GDNF-R-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-14045-img0008.jpg)
![Immunohistochemistry-Paraffin: GFR alpha-1/GDNF R alpha-1 Antibody [NBP2-14045] - Staining of human smooth muscle shows strong granular cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/GFR-alpha-1-GDNF-R-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-14045-img0004.jpg)
![Immunohistochemistry-Paraffin: GFR alpha-1/GDNF R alpha-1 Antibody [NBP2-14045] - Staining of human prostate shows strong granular cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/GFR-alpha-1-GDNF-R-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-14045-img0005.jpg)
![Immunohistochemistry-Paraffin: GFR alpha-1/GDNF R alpha-1 Antibody [NBP2-14045] - Staining of human colon shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/GFR-alpha-1-GDNF-R-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-14045-img0006.jpg)
![Immunohistochemistry-Paraffin: GFR alpha-1/GDNF R alpha-1 Antibody [NBP2-14045] - Staining of human heart muscle shows moderate cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/GFR-alpha-1-GDNF-R-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-14045-img0007.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (CL0178) [NBP2-52872] - Staining in human cerebral cortex and liver tissues. Corresponding AQP4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-CL0178-Immunohistochemistry-Paraffin-NBP2-52872-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (CL0178) [NBP2-52872] - Staining in human cerebral cortex and liver tissues. Corresponding AQP4 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Aquaporin-4 Antibody (CL0178) [NBP2-52872] - Analysis in human cerebral cortex tissue.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-CL0178-Western-Blot-NBP2-52872-img0013.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (CL0178) [NBP2-52872] - Staining of human cerebral cortex shows moderate to strong membranous positivity in astrocytes.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-CL0178-Immunohistochemistry-Paraffin-NBP2-52872-img0008.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (CL0178) [NBP2-52872] - Staining of human cerebellum shows strong positivity in neuropil, mainly in the molecular layer.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-CL0178-Immunohistochemistry-Paraffin-NBP2-52872-img0009.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (CL0178) [NBP2-52872] - Staining of human stomach shows moderate to strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-CL0178-Immunohistochemistry-Paraffin-NBP2-52872-img0010.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (CL0178) [NBP2-52872] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-CL0178-Immunohistochemistry-Paraffin-NBP2-52872-img0011.jpg)
![Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody [NBP2-34131] - Staining of human cerebral cortex, colon, eye, retina and lymph node using Anti-VIAAT/SLC32A1/VGAT antibody NBP2-34131 (A) shows similar protein distribution across tissues to independent antibody NBP2-34117 (B).](http://images.novusbio.com/fullsize/VIAAT-SLC32A1-VGAT-Antibody-Immunohistochemistry-Paraffin-NBP2-34131-img0017.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody [NBP2-34131] - Staining of human cerebral cortex, colon, eye, retina and lymph node using Anti-VIAAT/SLC32A1/VGAT antibody NBP2-34131 (A) shows similar protein distribution across tissues to independent antibody NBP2-34117 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody [NBP2-34131] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/VIAAT-SLC32A1-VGAT-Antibody-Immunohistochemistry-Paraffin-NBP2-34131-img0008.jpg)
![Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody [NBP2-34131] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/VIAAT-SLC32A1-VGAT-Antibody-Immunohistochemistry-Paraffin-NBP2-34131-img0009.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody [NBP2-34131] - Staining in human cerebral cortex and pancreas tissues using anti-VGAT antibody. Corresponding VGAT RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/VIAAT-SLC32A1-VGAT-Antibody-Immunohistochemistry-Paraffin-NBP2-34131-img0011.jpg)
![Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody [NBP2-34131] - Staining of human lymph node.](http://images.novusbio.com/fullsize/VIAAT-SLC32A1-VGAT-Antibody-Immunohistochemistry-Paraffin-NBP2-34131-img0012.jpg)
![Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody [NBP2-34131] - staining of human eye, retina.](http://images.novusbio.com/fullsize/VIAAT-SLC32A1-VGAT-Antibody-Immunohistochemistry-Paraffin-NBP2-34131-img0014.jpg)
![Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody [NBP2-34131] - Staining of human colon.](http://images.novusbio.com/fullsize/VIAAT-SLC32A1-VGAT-Antibody-Immunohistochemistry-Paraffin-NBP2-34131-img0013.jpg)
![Immunohistochemistry-Paraffin: Chromogranin C Antibody [NBP2-62693] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/Chromogranin-C-Antibody-Immunohistochemistry-Paraffin-NBP2-62693-img0007.jpg "Immunohistochemistry-Paraffin: Chromogranin C Antibody [NBP2-62693] - Staining of human cerebral cortex.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Chromogranin C Antibody [NBP2-62693] - Analysis in human adrenal gland and liver tissues using Anti-SCG2 antibody. Corresponding SCG2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Chromogranin-C-Antibody-Immunohistochemistry-Paraffin-NBP2-62693-img0001.jpg)
![Immunohistochemistry-Paraffin: Chromogranin C Antibody [NBP2-62693] - Staining of human adrenal gland shows high expression.](http://images.novusbio.com/fullsize/Chromogranin-C-Antibody-Immunohistochemistry-Paraffin-NBP2-62693-img0002.jpg)
![Immunohistochemistry-Paraffin: Chromogranin C Antibody [NBP2-62693] - Staining of human anterior pituitary gland shows strong cytoplasmic positivity in endocrine cells.](http://images.novusbio.com/fullsize/Chromogranin-C-Antibody-Immunohistochemistry-Paraffin-NBP2-62693-img0003.jpg)
![Immunohistochemistry-Paraffin: Chromogranin C Antibody [NBP2-62693] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/Chromogranin-C-Antibody-Immunohistochemistry-Paraffin-NBP2-62693-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Chromogranin C Antibody [NBP2-62693] - Staining of human adrenal gland, cerebral cortex, lymph node and pituitary gland using Anti-SCG2 antibody NBP2-62693 (A) shows similar protein distribution across tissues to independent antibody NBP1-85631 (B).](http://images.novusbio.com/fullsize/Chromogranin-C-Antibody-Immunohistochemistry-Paraffin-NBP2-62693-img0005.jpg)
![Immunohistochemistry-Paraffin: Chromogranin C Antibody [NBP2-62693] - Staining of human lymph node.](http://images.novusbio.com/fullsize/Chromogranin-C-Antibody-Immunohistochemistry-Paraffin-NBP2-62693-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Plexin A1 Antibody [NBP1-82544] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & cytosol.](http://images.novusbio.com/fullsize/Plexin-A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82544-img0005.jpg "Immunocytochemistry/Immunofluorescence: Plexin A1 Antibody [NBP1-82544] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & cytosol.")
![Immunohistochemistry-Paraffin: Plexin A1 Antibody [NBP1-82544] - Staining of human skeletal muscle shows no positivity in myocytes.](http://images.novusbio.com/fullsize/Plexin-A1-Antibody-Immunohistochemistry-Paraffin-NBP1-82544-img0010.jpg)
![Immunohistochemistry-Paraffin: Plexin A1 Antibody [NBP1-82544] - Staining of human lung shows strong cytoplasmic postivity in macrophages.](http://images.novusbio.com/fullsize/Plexin-A1-Antibody-Immunohistochemistry-Paraffin-NBP1-82544-img0006.jpg)
![Immunohistochemistry-Paraffin: Plexin A1 Antibody [NBP1-82544] - Staining of human cerebellum shows moderate cytoplsmic postivity in Purkinje cells.](http://images.novusbio.com/fullsize/Plexin-A1-Antibody-Immunohistochemistry-Paraffin-NBP1-82544-img0007.jpg)
![Immunohistochemistry-Paraffin: Plexin A1 Antibody [NBP1-82544] - Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Plexin-A1-Antibody-Immunohistochemistry-Paraffin-NBP1-82544-img0008.jpg)
![Immunohistochemistry-Paraffin: Plexin A1 Antibody [NBP1-82544] - Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Plexin-A1-Antibody-Immunohistochemistry-Paraffin-NBP1-82544-img0009.jpg)
![Immunohistochemistry-Paraffin: CD133 Antibody [NBP2-59789] - Staining in human cervix, uterine and liver tissues using NBP2-59789 antibody. Corresponding PROM1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD133-Antibody-Immunohistochemistry-Paraffin-NBP2-59789-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD133 Antibody [NBP2-59789] - Staining in human cervix, uterine and liver tissues using NBP2-59789 antibody. Corresponding PROM1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: CD133 Antibody [NBP2-59789] - Analysis in human cell line CACO-2 and human cell line PC-3.](http://images.novusbio.com/fullsize/CD133-Antibody-Western-Blot-NBP2-59789-img0005.jpg)
![Immunohistochemistry-Paraffin: CD133 Antibody [NBP2-59789] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/CD133-Antibody-Immunohistochemistry-Paraffin-NBP2-59789-img0011.jpg)
![Immunohistochemistry-Paraffin: CD133 Antibody [NBP2-59789] - Staining of human breast shows moderate to strong membranous and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CD133-Antibody-Immunohistochemistry-Paraffin-NBP2-59789-img0006.jpg)
![Immunohistochemistry-Paraffin: CD133 Antibody [NBP2-59789] - Staining of human cervix shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/CD133-Antibody-Immunohistochemistry-Paraffin-NBP2-59789-img0007.jpg)
![Immunohistochemistry-Paraffin: CD133 Antibody [NBP2-59789] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/CD133-Antibody-Immunohistochemistry-Paraffin-NBP2-59789-img0008.jpg)
![Immunohistochemistry-Paraffin: ELAVL2 Antibody [NBP2-38012] - Staining in human cerebral cortex and skeletal muscle tissues using NBP2-38012 antibody. Corresponding ELAVL2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ELAVL2-Antibody-Immunohistochemistry-Paraffin-NBP2-38012-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ELAVL2 Antibody [NBP2-38012] - Staining in human cerebral cortex and skeletal muscle tissues using NBP2-38012 antibody. Corresponding ELAVL2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ELAVL2 Antibody [NBP2-38012] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ELAVL2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38012-img0002.jpg)
![Immunohistochemistry-Paraffin: ELAVL2 Antibody [NBP2-38012] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/ELAVL2-Antibody-Immunohistochemistry-Paraffin-NBP2-38012-img0004.jpg)
![Immunohistochemistry-Paraffin: ELAVL2 Antibody [NBP2-38012] - Staining of human endometrium shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/ELAVL2-Antibody-Immunohistochemistry-Paraffin-NBP2-38012-img0006.jpg)
![Immunohistochemistry-Paraffin: ELAVL2 Antibody [NBP2-38012] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/ELAVL2-Antibody-Immunohistochemistry-Paraffin-NBP2-38012-img0007.jpg)
![Immunohistochemistry-Paraffin: ELAVL2 Antibody [NBP2-38012] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/ELAVL2-Antibody-Immunohistochemistry-Paraffin-NBP2-38012-img0008.jpg)
![Immunohistochemistry-Paraffin: Kynurenine 3-Monooxygenase/KMO Antibody [NBP2-54915] - Immunohistochemical staining of human kidney shows strong cytopalsmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Kynurenine-3-Monooxygenase-KMO-Antibody-Immunohistochemistry-Paraffin-NBP2-54915-img0001.jpg "Immunohistochemistry-Paraffin: Kynurenine 3-Monooxygenase/KMO Antibody [NBP2-54915] - Immunohistochemical staining of human kidney shows strong cytopalsmic positivity in cells in tubules.")
![Western Blot: RBFOX3/NeuN Antibody (5A4B9) [NBP2-61841] - Analysis using RBFOX3 mAb against HEK293 (1) and RBFOX3 (AA: 1-140)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-5A4B9-Western-Blot-NBP2-61841-img0005.jpg "Western Blot: RBFOX3/NeuN Antibody (5A4B9) [NBP2-61841] - Analysis using RBFOX3 mAb against HEK293 (1) and RBFOX3 (AA: 1-140)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: RBFOX3/NeuN Antibody (5A4B9) [NBP2-61841] - Analysis of Hela cells using RBFOX3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-5A4B9-Immunocytochemistry-Immunofluorescence-NBP2-61841-img0003.jpg)
![Flow Cytometry: RBFOX3/NeuN Antibody (5A4B9) [NBP2-61841] - Analysis of Hela cells using RBFOX3 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-5A4B9-Flow-Cytometry-NBP2-61841-img0002.jpg)
![Western Blot: RBFOX3/NeuN Antibody (5A4B9) [NBP2-61841] - Analysis using RBFOX3 mAb against human *** (AA: 1-140) recombinant protein. (Expected MW is 41.4 kDa)](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-5A4B9-Western-Blot-NBP2-61841-img0004.jpg)
![Western Blot: RBFOX3/NeuN Antibody (5A4B9) [NBP2-61841] - Analysis using RBFOX3 mouse mAb against C2C12 (1), RAW264.7 (2), and U251 (3) cell lysate.](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-5A4B9-Western-Blot-NBP2-61841-img0006.jpg)
![ELISA: RBFOX3/NeuN Antibody (5A4B9) [NBP2-61841] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-5A4B9-ELISA-NBP2-61841-img0001.jpg)
![Western Blot: RBFOX3/NeuN Antibody (1H3B2) [NBP2-61848] - Analysis using RBFOX3 mAb against HEK293 (1) and RBFOX3 (AA: 1-140)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-1H3B2-Western-Blot-NBP2-61848-img0005.jpg "Western Blot: RBFOX3/NeuN Antibody (1H3B2) [NBP2-61848] - Analysis using RBFOX3 mAb against HEK293 (1) and RBFOX3 (AA: 1-140)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: RBFOX3/NeuN Antibody (1H3B2) [NBP2-61848] - Analysis of Hela cells using RBFOX3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-1H3B2-Immunocytochemistry-Immunofluorescence-NBP2-61848-img0003.jpg)
![Western Blot: RBFOX3/NeuN Antibody (1H3B2) [NBP2-61848] - Analysis using RBFOX3 mAb against human RBFOX3 (AA: 1-140) recombinant protein. (Expected MW is 41.4 kDa)](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-1H3B2-Western-Blot-NBP2-61848-img0004.jpg)
![Western Blot: RBFOX3/NeuN Antibody (1H3B2) [NBP2-61848] - Analysis using RBFOX3 mouse mAb against C2C12 (1), RAW264.7 (2), and U251 (3) cell lysate.](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-1H3B2-Western-Blot-NBP2-61848-img0006.jpg)
![ELISA: RBFOX3/NeuN Antibody (1H3B2) [NBP2-61848] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-1H3B2-ELISA-NBP2-61848-img0001.jpg)
![ELISA: RBFOX3/NeuN Antibody (1H3B2) [NBP2-61848] - Analysis of Hela cells using RBFOX3 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-1H3B2-ELISA-NBP2-61848-img0002.jpg)
![Western Blot: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - Western Blot analysis of Human Alpha Synuclein showing detection of alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Monoclonal Antibody, Clone J18 (NBP3-18266). Lane 1: MW ladder. Lane 2: 0.5 ug human alpha synuclein monomer (SPR-321). Lane 3: 2 ug human alpha synuclein monomer (SPR-321). Lane 4: 0.5 ug human alpha synuclein PFFs (SPR-322). Lane 5: 2 ug human alpha synuclein PFFs (SPR-322). Lane 6: 15 ug human Parkinson's Disease brain lysate.. Block: 5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18266) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. NBP3-18266 does not detect unphosphorylated alpha synuclein.](http://images.novusbio.com/fullsize/alpha-Synuclein-p-Ser-129-Antibody-J18-Western-Blot-NBP3-18266-img0004.jpg "Western Blot: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - Western Blot analysis of Human Alpha Synuclein showing detection of alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Monoclonal Antibody, Clone J18 (NBP3-18266). Lane 1: MW ladder. Lane 2: 0.5 ug human alpha synuclein monomer (SPR-321). Lane 3: 2 ug human alpha synuclein monomer (SPR-321). Lane 4: 0.5 ug human alpha synuclein PFFs (SPR-322). Lane 5: 2 ug human alpha synuclein PFFs (SPR-322). Lane 6: 15 ug human Parkinson's Disease brain lysate.. Block: 5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18266) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. NBP3-18266 does not detect unphosphorylated alpha synuclein.")
![Immunohistochemistry: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - C57/BL6 mice were injected with sonicated recombinant mouse alpha synuclein monomers or fibrils at 8 weeks of age. Mice were unilaterally injected in the dorsal striatum (bregma AP + 0.2 mm, L +/1 2.0 mm, V - 3.0 mm) and sacrificed 30 days post-injection. (A) 1.25 uL mouse alpha synuclein monomers. (B) 2.5 uL mouse alpha synuclein monomers. (C) 2.5 ug alpha synuclein PFFs. (C) 5 ug alpha synuclein PFFs Inset: PBS (negative control). Primary antibody: Anti-alpha-Synuclein (NBP3-18266) at 1:10 000. Secondary antibody: anti-rabbit HRP. Mice injected with PFF displayed alpha synuclein staining in the striatum and cortex and contralateral to the injection site.](http://images.novusbio.com/fullsize/alpha-Synuclein-p-Ser-129-Antibody-J18-Immunohistochemistry-NBP3-18266-img0002.jpg)
![Western Blot: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - Western Blot analysis of Mouse Brain showing detection of alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Monoclonal Antibody, Clone J18 (NBP3-18266). Lane 1: MW ladder. Lane 2: Mouse brain. Load: 15 ug. Block: 5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18266) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Multiple bands due to phosphorylation.](http://images.novusbio.com/fullsize/alpha-Synuclein-p-Ser-129-Antibody-J18-Western-Blot-NBP3-18266-img0005.jpg)
![Immunohistochemistry: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - Immunohistochemistry analysis using Mouse Anti-alpha-Synuclein Monoclonal Antibody, Clone J18 (NBP3-18266). C57/BL6 mice were injected with 5 ug sonicated mouse recombinant alpha synuclein PFFs at 8 weeks of age. Mice were unilaterally injected in the dorsal striatum (bregma AP + 0.2 mm, L +/1 2.0 mm, V - 3.0 mm) and sacrificed 30 days post-injection. (A) contralateral cortex. (B) ipsilateral cortex. (C) contralateral striatum. (D) ipsilateral striatum. Primary Antibody: Mouse Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18266) at 1:10000. Secondary Antibody: anti-rabbit HRP. Courtesy of: Porsolt.](http://images.novusbio.com/fullsize/alpha-Synuclein-p-Ser-129-Antibody-J18-Immunohistochemistry-NBP3-18266-img0001.jpg)
![Dot Blot: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - Dot Blot analysis using Rabbit Anti-alpha-Synuclein Monoclonal Antibody, Clone J18 (NBP3-18266). Tissue: alpha synuclein peptide. Primary Antibody: Rabbit Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18266) at 1:500 for 2 hours at RT with shaking . Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking . Phospho peptide sequence: AYEMP-pS-EEGYQ. Non-phospho peptide sequence: AYEMPSEEGYQ. This sequence is the same for human, mouse, and rat.](http://images.novusbio.com/fullsize/alpha-Synuclein-p-Ser-129-Antibody-J18-Dot-Blot-NBP3-18266-img0003.jpg)