| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54593-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27301SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34808-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-46617 | |||
| BSA Free | NBP2-46617-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87103-25ul | |||
![Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1792) [NBP2-53408] - Formalin-fixed, paraffin-embedded Mouse Intestine stained with SOX2 Antibody (SOX2/1792).](http://images.novusbio.com/fullsize/SOX2-Antibody-SOX2-1792-Immunohistochemistry-Paraffin-NBP2-53408-img0005.jpg "Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1792) [NBP2-53408] - Formalin-fixed, paraffin-embedded Mouse Intestine stained with SOX2 Antibody (SOX2/1792).")
![Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1792) [NBP2-53408] - Formalin-fixed, paraffin-embedded Human Cervical Carcinoma stained with SOX2 Monoclonal Antibody (SOX2/1792).](http://images.novusbio.com/fullsize/SOX2-Antibody-SOX2-1792-Immunohistochemistry-Paraffin-NBP2-53408-img0001.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1792) [NBP2-53408] - Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with SOX2 Antibody (SOX2/1792).](http://images.novusbio.com/fullsize/SOX2-Antibody-SOX2-1792-Immunohistochemistry-Paraffin-NBP2-53408-img0003.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1792) [NBP2-53408] - Formalin-fixed, paraffin-embedded Mouse Stomach stained with SOX2 Antibody (SOX2/1792).](http://images.novusbio.com/fullsize/SOX2-Antibody-SOX2-1792-Immunohistochemistry-Paraffin-NBP2-53408-img0004.jpg)
![Protein Array: SOX2 Antibody (SOX2/1792) [NBP2-53408] - Analysis of Protein Array containing more than 19,000 full-length human proteins using SOX2 Antibody (SOX2/1792) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD?s) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD?s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/SOX2-Antibody-SOX2-1792-Protein-Array-NBP2-53408-img0006.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1792) [NBP2-53408] - Analysis of SOX2 stained human head-neck cancer sample. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-53408_mouse-monoclonal-sox2-antibody-sox2-1792-17720241044646.jpg)
![Western Blot: SOX2 Antibody (SOX2/1792) [NBP2-53408] - Western Blot of Recombinant SOX2 protein fragment and NCCIT cell lysate using SOX2 Antibody (SOX2/1792).](http://images.novusbio.com/fullsize/nbp2-53408_mouse-monoclonal-sox2-antibody-sox2-1792-17720241003232.jpg)
![Western Blot: Olig2 Antibody [NBP2-27301] - Mouse brain lysate was separated on a 12% gel using SDS-PAGE, protein transferred to PVDF membrane and blocked in 5% milk-TBST. The membrane was probed with 2 ug/mL Olig2 antibody in 1% milk and detected with an anti-rabbit HRP conjugated secondary using chemiluminescence.](http://images.novusbio.com/fullsize/Olig2-Antibody-Western-Blot-NBP2-27301-img0003.jpg "Western Blot: Olig2 Antibody [NBP2-27301] - Mouse brain lysate was separated on a 12% gel using SDS-PAGE, protein transferred to PVDF membrane and blocked in 5% milk-TBST. The membrane was probed with 2 ug/mL Olig2 antibody in 1% milk and detected with an anti-rabbit HRP conjugated secondary using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Olig2 Antibody [NBP2-27301] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with Olig2 NBP2-27301 at a 1:200 dilution overnight at 4 degrees Celsius and detected using Dylight 488 (Green). Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with Dylight 550 (Red). Nuclei were detected with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Olig2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-27301-img0002.jpg)
![Immunohistochemistry-Paraffin: Olig2 Antibody [NBP2-27301] - OLIG2 Antibody [NBP2-27301] - FFPE human cerebellum probed with OLIG2 antibody at 1:200.](http://images.novusbio.com/fullsize/OLIG2-Antibody-Immunohistochemistry-Paraffin-NBP2-27301-img0001.jpg)
![Western Blot: UCH-L1/PGP9.5 Antibody (SPM575) [NBP2-32896] - Western Blot Analysis of human brain tissue lysate using UCH-L1/PGP9.5 Antibody (SPM575).](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-SPM575-Western-Blot-NBP2-32896-img0003.jpg "Western Blot: UCH-L1/PGP9.5 Antibody (SPM575) [NBP2-32896] - Western Blot Analysis of human brain tissue lysate using UCH-L1/PGP9.5 Antibody (SPM575).")
![Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody (SPM575) [NBP2-32896] - Immunofluorescence of skin innervation using anti-PGP9.5. Antibody at dilution 1:500. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-SPM575-Immunocytochemistry-Immunofluorescence-NBP2-32896-img0002.jpg)
![Western Blot: HSP27 [p Ser78] Antibody [NBP2-24458] - Analysis of HSP27 (Ser78) in recombinant fusion protein containing 1) a phosphorylated serine at position 78 and 2) an unphosphorylated serine at position 78, using HSP27 phospho antibody at 0.01 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Hsp27-[p-Ser78]-Antibody-Western-Blot-NBP2-24458-img0001.jpg "Western Blot: HSP27 [p Ser78] Antibody [NBP2-24458] - Analysis of HSP27 (Ser78) in recombinant fusion protein containing 1) a phosphorylated serine at position 78 and 2) an unphosphorylated serine at position 78, using HSP27 phospho antibody at 0.01 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: DDR2 Antibody [NBP2-14926] - Human breast cancer, using DDR2 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/DDR2-Antibody-Immunohistochemistry-Paraffin-NBP2-14926-img0001.jpg "Immunohistochemistry-Paraffin: DDR2 Antibody [NBP2-14926] - Human breast cancer, using DDR2 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min")
![Western Blot: DDR2 Antibody [NBP2-14926] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DDR2 antibody diluted at 1:3000.](http://images.novusbio.com/fullsize/DDR2-Antibody-Western-Blot-NBP2-14926-img0004.jpg)
![Western Blot: DDR2 Antibody [NBP2-14926] - U87-MG whole cell and membrane extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DDR2 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/DDR2-Antibody-Western-Blot-NBP2-14926-img0005.jpg)
![Western Blot: DDR2 Antibody [NBP2-14926] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with DDR2 antibody [C2C3], C-term (NBP2-14926) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-14926_rabbit-polyclonal-ddr2-antibody-91020242063328.jpg)
![DDR2 antibody [C2C3], C-term detects DDR2 protein at cell membrane by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: DDR2 stained by DDR2 antibody [C2C3], C-term (NBP2-14926) diluted at 1:250.Red: Tau, an Axon marker, stained by Tau antibody [GT287] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-14926_rabbit-polyclonal-ddr2-antibody-immunocytochemistry-immunofluorescence-2611202518261315.jpg)
![<b>Genetic Strategies Validation. </b>Wild-type (WT) and DDR2 (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DDR2 antibody [C2C3], C-term (NBP2-14926) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-14926_rabbit-polyclonal-ddr2-antibody-western-blot-2611202518384019.jpg)
![Western Blot: PAK1 Antibody (4D1) [H00005058-M02] - Analysis of PAK1 expression in transfected 293T cell line by PAK1 monoclonal antibody (M02), clone 4D1.Lane 1: PAK1 transfected lysate (Predicted MW: 49.6 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PAK1-Antibody-4D1-Western-Blot-H00005058-M02-img0011.jpg "Western Blot: PAK1 Antibody (4D1) [H00005058-M02] - Analysis of PAK1 expression in transfected 293T cell line by PAK1 monoclonal antibody (M02), clone 4D1.Lane 1: PAK1 transfected lysate (Predicted MW: 49.6 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: PAK1 Antibody (4D1) [H00005058-M02] - Analysis of monoclonal antibody to PAK1 on HeLa cell. Antibody concentration 35 ug/ml.](http://images.novusbio.com/fullsize/PAK1-Antibody-4D1-Immunocytochemistry-Immunofluorescence-H00005058-M02-img0006.jpg)
![Immunohistochemistry-Paraffin: PAK1 Antibody (4D1) [H00005058-M02] - Analysis of monoclonal antibody to PAK1 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/PAK1-Antibody-4D1-Immunohistochemistry-Paraffin-H00005058-M02-img0008.jpg)
![Western Blot: PAK1 Antibody (4D1) [H00005058-M02] - Western Blot analysis of PAK1 expression in HeLa NE ( Cat # L013V3 ).](http://images.novusbio.com/fullsize/PAK1-Antibody-4D1-Western-Blot-H00005058-M02-img0001.jpg)
![Immunoprecipitation: PAK1 Antibody (4D1) [H00005058-M02] - Analysis of PAK1 transfected lysate using anti-PAK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PAK1 monoclonal antibody.](http://images.novusbio.com/fullsize/PAK1-Antibody-4D1-Immunoprecipitation-H00005058-M02-img0009.jpg)
![Sandwich ELISA: PAK1 Antibody (4D1) [H00005058-M02] - Detection limit for recombinant GST tagged PAK1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PAK1-Antibody-4D1-Sandwich-ELISA-H00005058-M02-img0010.jpg)
![Western Blot: Nrf1 Antibody (2F9) [H00004899-M01] - Analysis of NRF1 expression in transfected 293T cell line by NRF1 monoclonal antibody (M01), clone 2F9.Lane 1: NRF1 transfected lysate (Predicted MW: 55.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Nrf1-Antibody-2F9-Western-Blot-H00004899-M01-img0012.jpg "Western Blot: Nrf1 Antibody (2F9) [H00004899-M01] - Analysis of NRF1 expression in transfected 293T cell line by NRF1 monoclonal antibody (M01), clone 2F9.Lane 1: NRF1 transfected lysate (Predicted MW: 55.7 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: Nrf1 Antibody (2F9) [H00004899-M01] - Analysis of monoclonal antibody to NRF1 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Nrf1-Antibody-2F9-Immunohistochemistry-Paraffin-H00004899-M01-img0007.jpg)
![Western Blot: Nrf1 Antibody (2F9) [H00004899-M01] - Analysis of NRF1 over-expressed 293 cell line, cotransfected with NRF1 Validated Chimera RNAi ( Cat # H00004899-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NRF1 monoclonal antibody (M01), clone 2F9 (Cat # H00004899-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/Nrf1-Antibody-2F9-Western-Blot-H00004899-M01-img0008.jpg)
![Western Blot: Nrf1 Antibody (2F9) [H00004899-M01] - NRF1 monoclonal antibody (M01), clone 2F9. Analysis of NRF1 expression in HeLa.](http://images.novusbio.com/fullsize/Nrf1-Antibody-2F9-Western-Blot-H00004899-M01-img0010.jpg)
![Western Blot: Nrf1 Antibody (2F9) [H00004899-M01] - NRF1 monoclonal antibody (M01), clone 2F9. Analysis of NRF1 expression in human Skeletal muscle.](http://images.novusbio.com/fullsize/Nrf1-Antibody-2F9-Western-Blot-H00004899-M01-img0011.jpg)
![ELISA: Nrf1 Antibody (2F9) [H00004899-M01] - Detection limit for recombinant GST tagged NRF1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Nrf1-Antibody-2F9-ELISA-H00004899-M01-img0009.jpg)
![Immunocytochemistry: Olig2 Antibody (3C9) [H00010215-M03] - Imaging of Optic Nerve: Red-H00010215-M03(Olig2), Blue-DAPI. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/Olig2-Antibody-3C9-Immunocytochemistry-H00010215-M03-img0006.jpg "Immunocytochemistry: Olig2 Antibody (3C9) [H00010215-M03] - Imaging of Optic Nerve: Red-H00010215-M03(Olig2), Blue-DAPI. This image was submitted via customer Review.")
![Immunohistochemistry-Paraffin: Olig2 Antibody (3C9) [H00010215-M03] - Analysis of monoclonal antibody to OLIG2 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/Olig2-Antibody-3C9-Immunohistochemistry-Paraffin-H00010215-M03-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Olig2 Antibody (3C9) [H00010215-M03] - Analysis of monoclonal antibody to OLIG2 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/Olig2-Antibody-3C9-Immunocytochemistry-Immunofluorescence-H00010215-M03-img0004.jpg)
![Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/nbp3-43808_mouse-cnpase-mab-cl2887-azide-and-bsa-free-282202512154190.jpg "Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Mouse Cerebral Cortex tissue")
![Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/nbp3-43808_mouse-cnpase-mab-cl2887-azide-and-bsa-free-282202512145319.jpg)
![Western Blot: QKI/Quaking Antibody (GT1589) [NBP3-13575] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with QKI/Quaking antibody [GT1589] (NBP3-13575) diluted at 1:5000. The HRP-conjugated anti-mouset IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-GT1589-Western-Blot-NBP3-13575-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: QKI/Quaking Antibody (GT1589) [NBP3-13575] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with QKI/Quaking antibody [GT1589] (NBP3-13575) diluted at 1:5000. The HRP-conjugated anti-mouset IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Frozen: QKI/Quaking Antibody (GT1589) [NBP3-13575] - QKI/Quaking antibody [GT1589] detects QKI/Quaking protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: QKI/Quaking protein stained by QKI/Quaking antibody [GT1589] (NBP3-13575) diluted at 1:250. Red: Calbindin, stained by Calbindin antibody diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-GT1589-Immunohistochemistry-Frozen-NBP3-13575-img0005.jpg)
![Western Blot: QKI/Quaking Antibody (GT1589) [NBP3-13575] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with QKI/Quaking antibody [GT1589] (NBP3-13575) diluted at 1:3000.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-GT1589-Western-Blot-NBP3-13575-img0001.jpg)
![Immunohistochemistry-Paraffin: QKI/Quaking Antibody (GT1589) [NBP3-13575] - QKI/Quaking antibody [GT1589] detects QKI/Quaking protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. QKI/Quaking antibody [GT1589] (NBP3-13575) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-GT1589-Immunohistochemistry-Paraffin-NBP3-13575-img0002.jpg)
![Immunohistochemistry-Paraffin: QKI/Quaking Antibody (GT1589) [NBP3-13575] - QKI/Quaking antibody [GT1589] detects QKI/Quaking protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. QKI/Quaking antibody [GT1589] (NBP3-13575) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-GT1589-Immunohistochemistry-Paraffin-NBP3-13575-img0003.jpg)
![QKI antibody [GT1589] detects QKI protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: QKI protein stained by QKI antibody [GT1589] (NBP3-13575) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp3-13575_mouse-qki-quaking-mab-gt1589-immunocytochemistry-immunofluorescence-2611202518235322.jpg)
![Western Blot: S100A1 Antibody [NBP1-87103] - Analysis in control (vector only transfected HEK293T lysate) and S100A1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/S100A1-Antibody-Western-Blot-NBP1-87103-img0016.jpg "Western Blot: S100A1 Antibody [NBP1-87103] - Analysis in control (vector only transfected HEK293T lysate) and S100A1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: S100A1 Antibody [NBP1-87103] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/S100A1-Antibody-Immunohistochemistry-Paraffin-NBP1-87103-img0015.jpg)
![Western Blot: S100A1 Antibody [NBP1-87103] - Analysis in human cell line SK-MEL-30.](http://images.novusbio.com/fullsize/S100A1-Antibody-Western-Blot-NBP1-87103-img0007.jpg)
![Immunohistochemistry-Paraffin: S100A1 Antibody [NBP1-87103] - Staining of human heart muscle shows moderate to strong nuclear positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/S100A1-Antibody-Immunohistochemistry-Paraffin-NBP1-87103-img0011.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: S100A1 Antibody [NBP1-87103] - Staining in human heart muscle and lymph node tissues.. Corresponding S100A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/S100A1-Antibody-Immunohistochemistry-Paraffin-NBP1-87103-img0012.jpg)
![Immunohistochemistry-Paraffin: S100A1 Antibody [NBP1-87103] - Staining of human lymph node shows no positivity in non - germinal center cells as expected.](http://images.novusbio.com/fullsize/S100A1-Antibody-Immunohistochemistry-Paraffin-NBP1-87103-img0013.jpg)
![Immunohistochemistry-Paraffin: S100A1 Antibody [NBP1-87103] - Staining of human skin shows moderate to strong cytoplasmic positivity in melanocytes.](http://images.novusbio.com/fullsize/S100A1-Antibody-Immunohistochemistry-Paraffin-NBP1-87103-img0014.jpg)
![Western Blot: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-2120249422625.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - ZEB1 antibody [HL2245] detects ZEB1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZEB1 stained by ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-2120249374983.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - ZEB1 antibody [HL2245] detects ZEB1 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. ZEB1 stained by ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-2120249374923.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-2120249422673.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-2120249393552.jpg)
![Western Blot: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-212024941465.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - ZEB1 antibody [HL2245] detects ZEB1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. ZEB1 stained by ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-2120249415991.jpg)