| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70166 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03282-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-94458-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37446-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61885-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-13497-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61673-0.025ml | |||
![Western Blot: Doublecortin Antibody (BLR168J) [NBP3-14742] - Rabbit anti-Doublecortin recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Doublecortin-Antibody-BLR168J-Western-Blot-NBP3-14742-img0004.jpg "Western Blot: Doublecortin Antibody (BLR168J) [NBP3-14742] - Rabbit anti-Doublecortin recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. chemiluminescence with an exposure time of 30 seconds.")
![Immunocytochemistry: Doublecortin Antibody (BLR168J) [NBP3-14742] - Rabbit anti-Doublecortin recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG Substrate DAB](http://images.novusbio.com/fullsize/Doublecortin-Antibody-BLR168J-Immunocytochemistry-NBP3-14742-img0003.jpg)
![Immunohistochemistry-Paraffin: Doublecortin Antibody (BLR168J) [NBP3-14742] - Rabbit anti-Doublecortin recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate DAB](http://images.novusbio.com/fullsize/Doublecortin-Antibody-BLR168J-Immunohistochemistry-Paraffin-NBP3-14742-img0002.jpg)
![Immunohistochemistry-Paraffin: Doublecortin Antibody (BLR168J) [NBP3-14742] - Rabbit anti-Doublecortin recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate DAB](http://images.novusbio.com/fullsize/Doublecortin-Antibody-BLR168J-Immunohistochemistry-Paraffin-NBP3-14742-img0001.jpg)
![Immunoprecipitation- Doublecortin Antibody (BLR168J)-NBP3-14742-Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from SK-N-BE(2) cells prepared using NETN lysis buffer. Antibodies: Rabbit antiDoublecortin recombinant monoclonal antibody [BLR168J] used for IP at 20 ul/mg lysate. Doublecortin was also immunoprecipitated by a second antibody against a different epitope of Doublecortin (BL-1974A-2B1). For blotting immunoprecipitated Doublecortin was used at 1:1000. Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-14742_rabbit-doublecortin-mab-immunoprecipitation-2102023105019..jpg)
![Western Blot: IGF-I R/IGF1R Antibody (HL1957) - Azide and BSA Free [NBP3-25519] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with IGF1R antibody [HL1957] (NBP3-25519) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25519_rabbit-igf-i-r-igf1r-mab-hl1957-azide-and-bsa-free-212024936274.jpg "Western Blot: IGF-I R/IGF1R Antibody (HL1957) - Azide and BSA Free [NBP3-25519] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with IGF1R antibody [HL1957] (NBP3-25519) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: IGF-I R/IGF1R Antibody (HL1957) - Azide and BSA Free [NBP3-25519] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with IGF1R antibody [HL1957] (NBP3-25519) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25519_rabbit-igf-i-r-igf1r-mab-hl1957-azide-and-bsa-free-2120249422681.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: IGF-I R/IGF1R Antibody (HL1957) - Azide and BSA Free [NBP3-25519] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with IGF1R antibody [HL1957] (NBP3-25519) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25519_rabbit-igf-i-r-igf1r-mab-hl1957-azide-and-bsa-free-2120249425469.jpg)
![Immunocytochemistry/Immunofluorescence: IGF-I R/IGF1R Antibody (HL1957) - Azide and BSA Free [NBP3-25519] - IGF1R antibody [HL1957] detects IGF1R protein at cell membrane by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IGF1R stained by IGF1R antibody [HL1957] (NBP3-25519) diluted at 1:1000. Blue: Fluoroshield with DAPI . Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp3-25519_rabbit-igf-i-r-igf1r-mab-hl1957-azide-and-bsa-free-2120249425455.jpg)
![Immunohistochemistry-Paraffin: IGF-I R/IGF1R Antibody (HL1957) - Azide and BSA Free [NBP3-25519] - IGF1R antibody [HL1957] detects IGF1R protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. IGF1R stained by IGF1R antibody [HL1957] (NBP3-25519) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25519_rabbit-igf-i-r-igf1r-mab-hl1957-azide-and-bsa-free-2120249422643.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: IGF-I R/IGF1R Antibody (HL1957) - Azide and BSA Free [NBP3-25519] - Wild-type (WT) and IGF1R knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with IGF1R antibody [HL1957] (NBP3-25519) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25519_rabbit-igf-i-r-igf1r-mab-hl1957-azide-and-bsa-free-212024940886.jpg)
![Western Blot: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY ALOX5 (RC217259, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiALOX5.(1:2000)](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-3F1-Western-Blot-NBP2-46513-img0003.jpg "Western Blot: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY ALOX5 (RC217259, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiALOX5.(1:2000)")
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - Paraffin-embedded Adenocarcinoma of Human ovary tissue using antiALOX5 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150)](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-3F1-Immunohistochemistry-Paraffin-NBP2-46513-img0014.jpg)
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - Paraffin-embedded Human colon tissue using anti-ALOX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150).](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-3F1-Immunohistochemistry-Paraffin-NBP2-46513-img0005.jpg)
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - Paraffin-embedded Human Kidney tissue using anti-ALOX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,(1:150)](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-3F1-Immunohistochemistry-Paraffin-NBP2-46513-img0007.jpg)
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - Paraffin-embedded Carcinoma of Human kidney tissue using anti-ALOX5 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min (1:150)](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-3F1-Immunohistochemistry-Paraffin-NBP2-46513-img0010.jpg)
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - Paraffin-embedded Human liver tissue using anti-ALOX5 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,1:150).](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-3F1-Immunohistochemistry-Paraffin-NBP2-46513-img0015.jpg)
![Immunohistochemistry-Paraffin: CRIPTO Antibody [NBP1-68841] - Cripto1 Antibody [NBP1-68841] (3.8ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/CRIPTO-Antibody-Immunohistochemistry-Paraffin-NBP1-68841-img0002.jpg "Immunohistochemistry-Paraffin: CRIPTO Antibody [NBP1-68841] - Cripto1 Antibody [NBP1-68841] (3.8ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody [NBP3-03282] - Analysis of extracts of various cell lines, using Gastrin-releasing Peptide/Bombesin/Neuromedin C antibody (NBP3-03282) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Gastrin-releasing-Peptide-Bombesin-Neuromedin-C-Antibody-Western-Blot-NBP3-03282-img0004.jpg "Western Blot: Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody [NBP3-03282] - Analysis of extracts of various cell lines, using Gastrin-releasing Peptide/Bombesin/Neuromedin C antibody (NBP3-03282) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin: Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody [NBP3-03282] - Mouse heart using Gastrin-releasing Peptide/Bombesin/Neuromedin C Rabbit pAb (NBP3-03282) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Gastrin-releasing-Peptide-Bombesin-Neuromedin-C-Antibody-Immunohistochemistry-Paraffin-NBP3-03282-img0003.jpg)
![Western Blot: Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody [NBP3-03282] - Analysis of extracts of various cell lines, using Gastrin-releasing Peptide/Bombesin/Neuromedin C antibody (NBP3-03282) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Gastrin-releasing Peptide-Bombesin-Neuromedin C Antibody-Western Blot-NBP3-03282-img0005.jpg)
![Immunohistochemistry-Paraffin: Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody [NBP3-03282] - Rat brain using Gastrin-releasing Peptide/Bombesin/Neuromedin C Rabbit pAb (NBP3-03282) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Gastrin-releasing-Peptide-Bombesin-Neuromedin-C-Antibody-Immunohistochemistry-Paraffin-NBP3-03282-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody - BSA Free [Gastrin-releasing Peptide/Bombesin/Neuromedin C] - Immunofluorescence analysis of NIH/3T3 cells using Gastrin-releasing Peptide/Bombesin/Neuromedin C Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03282_rabbit-polyclonal-gastrin-releasing-peptide-bombesin-neuromedin-c-antibody-52202516345228.jpg)
![Immunocytochemistry/ Immunofluorescence: Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody - BSA Free [Gastrin-releasing Peptide/Bombesin/Neuromedin C] - Immunofluorescence analysis of PC-12 cells using Gastrin-releasing Peptide/Bombesin/Neuromedin C Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03282_rabbit-polyclonal-gastrin-releasing-peptide-bombesin-neuromedin-c-antibody-5220251639613.jpg)
![Western Blot: Cathepsin L/V/K/H Antibody (2Q10F10) [NBP3-16524] - Western blot analysis of extracts of various cell lines, using Cathepsin L/V/K/H Rabbit mAb (NBP3-16524) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Cathepsin-L-V-K-H-Antibody-2Q10F10-Western-Blot-NBP3-16524-img0003.jpg "Western Blot: Cathepsin L/V/K/H Antibody (2Q10F10) [NBP3-16524] - Western blot analysis of extracts of various cell lines, using Cathepsin L/V/K/H Rabbit mAb (NBP3-16524) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: Cathepsin L/V/K/H Antibody (2Q10F10) [NBP3-16524] - Immunohistochemistry of paraffin-embedded mouse liver using Cathepsin L/V/K/H Rabbit mAb (NBP3-16524) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Cathepsin-L-V-K-H-Antibody-2Q10F10-Immunohistochemistry-Paraffin-NBP3-16524-img0002.jpg)
![Immunohistochemistry-Paraffin: Cathepsin L/V/K/H Antibody (2Q10F10) [NBP3-16524] - Immunohistochemistry of paraffin-embedded human liver cancer using Cathepsin L/V/K/H Rabbit mAb (NBP3-16524) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Cathepsin-L-V-K-H-Antibody-2Q10F10-Immunohistochemistry-Paraffin-NBP3-16524-img0001.jpg)
![Immunohistochemistry: Cathepsin L/V/K/H Antibody (2Q10F10) [NBP3-16524] - Immunohistochemistry analysis of Cathepsin L/V/K/H in paraffin-embedded mouse kidney tissue using Cathepsin L/V/K/H Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16524_rabbit-cathepsin-l-v-k-h-mab-2q10f10-72202512432081.jpg)
![Immunohistochemistry: Cathepsin L/V/K/H Antibody (2Q10F10) [NBP3-16524] - Immunohistochemistry analysis of Cathepsin L/V/K/H in paraffin-embedded rat brain tissue using Cathepsin L/V/K/H Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16524_rabbit-cathepsin-l-v-k-h-mab-2q10f10-72202512435344.jpg)
![Immunohistochemistry: Cathepsin L/V/K/H Antibody (2Q10F10) [NBP3-16524] - Immunohistochemistry analysis of Cathepsin L/V/K/H in paraffin-embedded human liver tissue using Cathepsin L/V/K/H Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16524_rabbit-cathepsin-l-v-k-h-mab-2q10f10-722025124532.jpg)
![Immunohistochemistry: Cathepsin L/V/K/H Antibody (2Q10F10) [NBP3-16524] - Immunohistochemistry analysis of Cathepsin L/V/K/H in paraffin-embedded human liver cancer tissue using Cathepsin L/V/K/H Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16524_rabbit-cathepsin-l-v-k-h-mab-2q10f10-72202512435325.jpg)
![Western Blot: MTCO1 Antibody [NBP2-94458] - Analysis of extracts of various cell lines, using MTCO1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/MTCO1-Antibody-Western-Blot-NBP2-94458-img0012.jpg "Western Blot: MTCO1 Antibody [NBP2-94458] - Analysis of extracts of various cell lines, using MTCO1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry: MTCO1 Antibody - Azide and BSA Free [NBP2-94458] - Immunohistochemistry analysis of paraffin-embedded rat brain using MTCO1 Rabbit pAb (A17889) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp2-94458_rabbit-polyclonal-mtco1-antibody-52202519302978.jpg)
![Immunocytochemistry/ Immunofluorescence: MTCO1 Antibody - Azide and BSA Free [NBP2-94458] - Immunofluorescence analysis of PC-12 cells using MTCO1 Rabbit pAb (A17889) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94458_rabbit-polyclonal-mtco1-antibody-52202519313440.jpg)
![Immunohistochemistry: MTCO1 Antibody - Azide and BSA Free [NBP2-94458] - Immunohistochemistry analysis of paraffin-embedded rat kidney using MTCO1 Rabbit pAb (A17889) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp2-94458_rabbit-polyclonal-mtco1-antibody-52202519313412.jpg)
![Immunohistochemistry: MTCO1 Antibody - Azide and BSA Free [NBP2-94458] - Immunohistochemistry analysis of paraffin-embedded mouse heart using MTCO1 Rabbit pAb (A17889) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp2-94458_rabbit-polyclonal-mtco1-antibody-522025193134.jpg)
![Immunohistochemistry: MTCO1 Antibody - Azide and BSA Free [NBP2-94458] - Immunohistochemistry analysis of paraffin-embedded mouse kidney using MTCO1 Rabbit pAb (A17889) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp2-94458_rabbit-polyclonal-mtco1-antibody-52202519302999.jpg)
![Western Blot: Hyaluronan synthase 2 Antibody (4E7) [NBP2-37446] - Western blot analysis using HAS2 mouse mAb against NTERA-2 (1), HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Hyaluronan-synthase-2-Antibody-4E7-Western-Blot-NBP2-37446-img0003.jpg "Western Blot: Hyaluronan synthase 2 Antibody (4E7) [NBP2-37446] - Western blot analysis using HAS2 mouse mAb against NTERA-2 (1), HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Hyaluronan synthase 2 Antibody (4E7) [NBP2-37446] - Immunofluorescence analysis of HeLa cells using HAS2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Hyaluronan-synthase-2-Antibody-4E7-Immunocytochemistry-Immunofluorescence-NBP2-37446-img0004.jpg)
![Immunohistochemistry-Paraffin: Hyaluronan synthase 2 Antibody (4E7) [NBP2-37446] - Analysis of ovarian cancer tissues using HAS2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Hyaluronan-synthase-2-Antibody-4E7-Immunohistochemistry-NBP2-37446-img0005.jpg)
![Western Blot: Hyaluronan synthase 2 Antibody (4E7) [NBP2-37446] - Western blot analysis using HAS2 mAb against human HAS2 recombinant protein. (Expected MW is 37.5 kDa)](http://images.novusbio.com/fullsize/Hyaluronan-synthase-2-Antibody-4E7-Western-Blot-NBP2-37446-img0002.jpg)
![ELISA: Hyaluronan synthase 2 Antibody (4E7) [NBP2-37446] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Hyaluronan-synthase-2-Antibody-4E7-ELISA-NBP2-37446-img0001.jpg)
![Western Blot: SOD2/Mn-SOD Antibody (8H3F9) [NBP2-61885] - Analysis using SOD2 mAb against HEK293 (1) and SOD2 (AA: 1-222)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-8H3F9-Western-Blot-NBP2-61885-img0006.jpg "Western Blot: SOD2/Mn-SOD Antibody (8H3F9) [NBP2-61885] - Analysis using SOD2 mAb against HEK293 (1) and SOD2 (AA: 1-222)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry: SOD2/Mn-SOD Antibody (8H3F9) [NBP2-61885] - Analysis of paraffin-embedded rectum cancer tissues using SOD2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-8H3F9-Immunohistochemistry-NBP2-61885-img0004.jpg)
![Flow Cytometry: SOD2/Mn-SOD Antibody (8H3F9) [NBP2-61885] - Analysis of MCF-7 cells using SOD2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-8H3F9-Flow-Cytometry-NBP2-61885-img0002.jpg)
![Western Blot: SOD2/Mn-SOD Antibody (8H3F9) [NBP2-61885] - Analysis using SOD2 mAb against human SOD2 (AA: 1-222) recombinant protein. (Expected MW is 50.7 kDa)](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-8H3F9-Western-Blot-NBP2-61885-img0005.jpg)
![Western Blot: SOD2/Mn-SOD Antibody (8H3F9) [NBP2-61885] - Analysis using SOD2 mouse mAb against Hela (1), HepG2 (2), and SH-SY5Y (3) cell lysate.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-8H3F9-Western-Blot-NBP2-61885-img0007.jpg)
![Immunohistochemistry: SOD2/Mn-SOD Antibody (8H3F9) [NBP2-61885] - Analysis of paraffin-embedded breast cancer tissues using SOD2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-8H3F9-Immunohistochemistry-NBP2-61885-img0003.jpg)
![ELISA: SOD2/Mn-SOD Antibody (8H3F9) [NBP2-61885] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-8H3F9-ELISA-NBP2-61885-img0001.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody [NB120-13497] - Western blot analysis of Rat Brain cell lysates showing detection of ~22 kDa AlphaB Crystallin/CRYAB protein using Rabbit Anti-AlphaB Crystallin/CRYAB Polyclonal Antibody (NB120-13497). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-AlphaB Crystallin/CRYAB Polyclonal Antibody (NB120-13497) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~22 kDa.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Western-Blot-NB120-13497-img0009.jpg "Western Blot: AlphaB Crystallin/CRYAB Antibody [NB120-13497] - Western blot analysis of Rat Brain cell lysates showing detection of ~22 kDa AlphaB Crystallin/CRYAB protein using Rabbit Anti-AlphaB Crystallin/CRYAB Polyclonal Antibody (NB120-13497). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-AlphaB Crystallin/CRYAB Polyclonal Antibody (NB120-13497) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~22 kDa.")
![Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody [NB120-13497] - analysis using Rabbit Anti-Alpha B Crystallin Polyclonal Antibody . Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Alpha B Crystallin Polyclonal Antibody at 1:120 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Actin filament bundles. Nuclear splicing speckles. Exosomes. Magnification: 20x. Heat Shocked at 42C for 1h.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Immunocytochemistry-Immunofluorescence-NB120-13497-img0002.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody [NB120-13497] - Western blot analysis of Human A431, HCT116, HeLa, HepG2, HEK293, HUVEC, Jurkat, MCF7, PC3 and T98G cell lysates showing detection of ~22 kDa AlphaB Crystallin/CRYAB protein using Rabbit Anti-AlphaB Crystallin/CRYAB Polyclonal Antibody (NB120-13497). Lane 1: Molecular Weight Ladder (MW). Lane 2: A431 cell lysates. Lane 3: HCT116 cell lysates. Lane 4: HeLa cell lysates. Lane 5: HepG2 cell lysates. Lane 6: HEK293 cell lysates. Lane 7: HUVEC cell lysates. Lane 8: Jurkat cell lysates. Lane 9: MCF7 cell lysates. Lane 10: PC3 cell lysates. Lane 11: T98G cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-AlphaB Crystallin/CRYAB Polyclonal Antibody (NB120-13497) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~22 kDa.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Western-Blot-NB120-13497-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody [NB120-13497] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Alpha B Crystallin Polyclonal Antibody at 1:120 for 12 hours at 4C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Actin filament bundles. Nuclear splicing speckles. Exosomes. Magnification: 100x. Heat Shocked at 42C for 1h.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Immunocytochemistry-Immunofluorescence-NB120-13497-img0003.jpg)
![Western Blot: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis using CHRNA3 mAb against human CHRNA3 (AA: 32-240) recombinant protein. (Expected MW is 50.6 kDa)](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-3-CHRNA3-Antibody-6D3A10-Western-Blot-NBP2-61673-img0005.jpg "Western Blot: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis using CHRNA3 mAb against human CHRNA3 (AA: 32-240) recombinant protein. (Expected MW is 50.6 kDa)")
![Immunocytochemistry/Immunofluorescence: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis of SMMC-7721 cells using CHRNA3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-3-CHRNA3-Antibody-6D3A10-Immunocytochemistry-Immunofluorescence-NBP2-61673-img0004.jpg)
![Flow Cytometry: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis of SK-N-SH cells using CHRNA3 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-3-CHRNA3-Antibody-6D3A10-Flow-Cytometry-NBP2-61673-img0007.jpg)
![Western Blot: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis using CHRNA3 mAb against HEK293 (1) and CHRNA3 (AA: 32-240)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-3-CHRNA3-Antibody-6D3A10-Western-Blot-NBP2-61673-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis of Hela cells using CHRNA3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-3-CHRNA3-Antibody-6D3A10-Immunocytochemistry-Immunofluorescence-NBP2-61673-img0003.jpg)
![Flow Cytometry: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis of SH-SY5Y cells using CHRNA3 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-3-CHRNA3-Antibody-6D3A10-Flow-Cytometry-NBP2-61673-img0002.jpg)
![ELISA: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-3-CHRNA3-Antibody-6D3A10-ELISA-NBP2-61673-img0001.jpg)
![Immunohistochemistry: Glut1 Antibody (SA0377) [NBP3-32385] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Glut1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32385, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-298202414262648.jpg "Immunohistochemistry: Glut1 Antibody (SA0377) [NBP3-32385] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Glut1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32385, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry: Glut1 Antibody (SA0377) [NBP3-32385] - Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Glut1 with Rabbit anti-Glut1 antibody (NBP3-32385) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32385, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-298202414333665.jpg)
![Immunocytochemistry/ Immunofluorescence: Glut1 Antibody (SA0377) [NBP3-32385] - Immunocytochemistry analysis of HeLa cells labeling Glut1 with Rabbit anti-Glut1 antibody (NBP3-32385) at 1/500 dilution.Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Glucose Transporter GLUT1 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-29202410334092.jpg)
![Flow Cytometry: Glut1 Antibody (SA0377) [NBP3-32385] - Flow cytometric analysis of Jurkat cells labeling Glut1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32385, red) at 1/1,000 dilution, compared with Rabbit IgG Isotype Control (blue). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with an iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-29202416372147.jpg)
![Western Blot: Glut1 Antibody (SA0377) [NBP3-32385] - Western blot analysis of Glut1 on different lysates with Rabbit anti-Glut1 antibody (NBP3-32385) at 1/50,000 dilution. Lane 1: HeLa cell lysate (no heat) Lane 2: HT-29 cell lysate (no heat) Lane 3: HepG2 cell lysate (no heat) Lane 4: NIH/3T3 cell lysate (no heat) Lane 5: L-929 cell lysate (no heat) Lane 6: Mouse brain tissue lysate (no heat) Lane 7: Rat brain tissue lysate (no heat) Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 45-60 kDa Exposure time: Lane 1-7 (left): 20 seconds; Lane 1-7 (right): 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/50,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-3920248453511.jpg)