| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76910-0.025mg | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Atto594 | NBP3-14765-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37261-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-47579-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-46853SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-82001-0.025ml | |||
![Western Blot: APP Antibody [NBP2-15575] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody diluted at 1:500.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP2-15575-img0008.jpg "Western Blot: APP Antibody [NBP2-15575] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody diluted at 1:500.")
![Immunohistochemistry-Paraffin: APP Antibody [NBP2-15575] - Rat brain. APP antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-Paraffin-NBP2-15575-img0003.jpg)
![Western Blot: APP Antibody [NBP2-15575] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody diluted at 1:500.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP2-15575-img0004.jpg)
![Western Blot: APP Antibody [NBP2-15575] - Human tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody diluted at 1:500.Human tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody diluted at 1:500.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP2-15575-img0006.jpg)
![Western Blot: APP Antibody [NBP2-15575] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody diluted at 1:500.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP2-15575-img0007.jpg)
![ELISA: APP Antibody [NBP2-15575] - Detection of non-transfected and transfected 293T whole cell extracts using NBP2-15575 as capture antibody at concentration of 5 ug/mL and a mouse monoclonal anti-APP antibody as detection antibody at concentration of 1 ug/mL. Mouse IgG antibody (HRP) (NBP2-19382) was diluted at 1:10000 and used to detect the primary antibody.](http://images.novusbio.com/fullsize/APP-Antibody-ELISA-NBP2-15575-img0013.jpg)
![Western Blot: APP Antibody [NBP1-76910] - Analysis of APP in mouse brain tissue lysate with APP antibody at (A) 0.5 and (B) 1 ug/ml.](http://images.novusbio.com/fullsize/APP-Antibody-Western-Blot-NBP1-76910-img0012.jpg "Western Blot: APP Antibody [NBP1-76910] - Analysis of APP in mouse brain tissue lysate with APP antibody at (A) 0.5 and (B) 1 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: APP Antibody - BSA Free [NBP1-76910] - Immunofluorescence of APP in Rat Brain cells with APP antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76910_rabbit-polyclonal-app-antibody-imgenex-img-191-2711202419451253.jpg)
![Immunocytochemistry/ Immunofluorescence: APP Antibody - BSA Free [NBP1-76910] - Immunofluorescence of APP in mouse brain tissue with APP Antibody at 20 ug/mL. Green: APP antibody Red: Phylloidin staining Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-76910_rabbit-polyclonal-app-antibody-imgenex-img-191-2711202419541571.gif)
![Immunohistochemistry: APP Antibody - BSA Free [NBP1-76910] - Immunohistochemistry of APP in mouse brain tissue with APP Antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76910_rabbit-polyclonal-app-antibody-imgenex-img-191-2711202419251561.gif)
![Immunohistochemistry: APP Antibody - BSA Free [NBP1-76910] - Immunohistochemical staining of rat brain using APP antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76910_rabbit-polyclonal-app-antibody-imgenex-img-191-271120241932669.jpg)
![Immunohistochemistry: GRK2 Antibody (3F8) [NBP2-37611] - Localization of G protein-coupled receptor kinase 2 (GRK2) in two patients with takotsubo syndrome who underwent endomyocardial biopsy in both the acute and recovery phases. Micrographs showing immunofluorescence stainings of GRK2 in the acute and recovery phases. Arrowheads (yellow) indicate positive signals for GRK2. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-31034-z) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GRK2-Antibody-3F8-Immunohistochemistry-NBP2-37611-img0006.jpg "Immunohistochemistry: GRK2 Antibody (3F8) [NBP2-37611] - Localization of G protein-coupled receptor kinase 2 (GRK2) in two patients with takotsubo syndrome who underwent endomyocardial biopsy in both the acute and recovery phases. Micrographs showing immunofluorescence stainings of GRK2 in the acute and recovery phases. Arrowheads (yellow) indicate positive signals for GRK2. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-31034-z) licensed under a CC-BY license.")
![Western Blot: GRK2 Antibody (3F8) [NBP2-37611] - Analysis using GRK2 mouse mAb against Hela (1), Jurkat (2), MOLT4 (3), RAJI (4), THP-1 (5), L1210 (6), Cos7 (7), PC-12 (8), and NIH/3T3 (9) cell lysate.](http://images.novusbio.com/fullsize/GRK2-Antibody-3F8-Western-Blot-NBP2-37611-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: GRK2 Antibody (3F8) [NBP2-37611] - Analysis of NIH/3T3 cells using GRK2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/GRK2-Antibody-3F8-Immunocytochemistry-Immunofluorescence-NBP2-37611-img0003.jpg)
![Immunohistochemistry: GRK2 Antibody (3F8) [NBP2-37611] - Localization of G protein-coupled receptor kinase 2 (GRK2). (a) Immunohistofluorescence staining for GRK2 using the specific antibody. GRK2 (red) in the takotsubo syndrome (TTS) and dilated cardiomyopathy (DCM) groups were observed not only in the cytoplasm but also on the cell membrane, which was confirmed by colocalization with wheat germ agglutinin (WGA) (green) (Arrowheads). Blue staining localized to 4',6-diamidino-2-phenylindole (DAPI). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-31034-z) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GRK2-Antibody-3F8-Immunohistochemistry-NBP2-37611-img0007.jpg)
![Immunohistochemistry-Paraffin: GRK2 Antibody (3F8) [NBP2-37611] - Analysis of endometrial cancer tissues (left) and cervical cancer tissues (right) using GRK2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/GRK2-Antibody-3F8-Immunohistochemistry-Paraffin-NBP2-37611-img0005.jpg)
![ELISA: GRK2 Antibody (3F8) [NBP2-37611] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/GRK2-Antibody-3F8-ELISA-NBP2-37611-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: GRK2 Antibody (3F8) - BSA Free [NBP2-37611] - Localization of G protein-coupled receptor kinase 2 (GRK2), beta -arrestin2, & 8-hydroxy-2′deoxyguanosine (8-OHdG) in two patients with takotsubo syndrome who underwent endomyocardial biopsy in both the acute & recovery phases. Micrographs showing immunofluorescence stainings of GRK2 & beta -arrestin2, & immunohistochemical staining of 8-OHdG in the acute & recovery phases. Arrowheads (yellow) indicate positive signals for GRK2 & beta -arrestin2 on the cell membrane, & Arrowheads (red) indicate 8-OHdG-positive nuclei. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30143703), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-37611_mouse-monoclonal-grk2-antibody-3f8-310202415371959.jpg)
![Immunocytochemistry/ Immunofluorescence: GRK2 Antibody (3F8) - BSA Free [NBP2-37611] - Localization of G protein-coupled receptor kinase 2 (GRK2). (a) Immunohistofluorescence staining for GRK2 using the specific antibody. GRK2 (red) in the takotsubo syndrome (TTS) & dilated cardiomyopathy (DCM) groups were observed not only in the cytoplasm but also on the cell membrane, which was confirmed by colocalization with wheat germ agglutinin (WGA) (green) (Arrowheads). Blue staining localized to 4′,6-diamidino-2-phenylindole (DAPI). (b) Quantification for GRK2 positive stained area in the myocardium. The box represents the 25th & 75th percentiles & the line the median value. Whiskers correspond to the 25th percentile minus 1.5 times interquartile range (IQR) & to the 75th percentile plus 1.5 IQR. (c) Quantification for membranous GRK2 positive cardiomyocyte. *P < 0.001 vs. the normal control (NC) group. †P < 0.001 vs. the DCM group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30143703), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-37611_mouse-monoclonal-grk2-antibody-3f8-310202416121924.jpg)
![Western Blot: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Lane 1: Molecular Weight Ladder (MW). Lane 2: Active Alpha Synuclein Protein Aggregate.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Western-Blot-NBP2-54789-img0005.jpg "Western Blot: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Lane 1: Molecular Weight Ladder (MW). Lane 2: Active Alpha Synuclein Protein Aggregate.")
![Immunocytochemistry/Immunofluorescence: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Primary rat hippocampal neurons show lewy body inclusion formation when treated with active Alpha Synuclein Protein Aggregate at 4 ug/ml (D-F), but not when treated with control Alpha Synuclein Protein Aggregate at 4 ug/ml (A-C). Tissue: Primary hippocampal neurons. Species: Sprague-Dawley rat. Fixation: 4% formaldehyde from PFA. Primary Antibody: Mouse anti-pSer129 Antibody at 1:1000 24 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:700 for 1 hours at RT. Counterstain: Hoechst (blue) nuclear stain at 1:4000 for 1 hour at RT. Localization: Lewy body incluscions. Magnification: 20x.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Immunocytochemistry-Immunofluorescence-NBP2-54789-img0004.jpg)
![Immunohistochemistry: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Immunohistochemistry analysis of rat brain injected with Type 1 human alpha synuclein PFFs (NBP2-54789). Species: Female Sprague-Dawley Rat. Rat was injected with 16g Type 1 human alpha synuclein PFFs (NBP2-54789) in each of 2 injection sites: AP+1.6, ML+2.4, DV-4.2 from skull; and AP-1.4, ML+0.2, DV-2.8 from skull. 30-days post-injection. Fixation: Saline perfusion followed by 4% PFA fixation for 48 hrs. Primary antibody: rabbit monoclonal anti-pSer129 alpha synuclein. Secondary Antibody: Biotin-SP Donkey Anti-Rabbit IgG (H+L) at 1:500 for 2 hours in cold room with shaking. ABC signal amplification, DAB staining. Magnification: 20X. Alpha synuclein pathology is seen in the striatum close to an injection site.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Immunohistochemistry-NBP2-54789-img0007.jpg)
![In vitro assay: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate (NBP2-54789) is combined with 100 uM of active alpha Synuclein monomer (NBP2-54788), as compared to active alpha Synuclein aggregate (NBP2-54789) and active alpha Synuclein monomer (NBP2-54788) alone. Thioflavin T ex = 450 nm, em = 485 nm.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-In-vitro-assay-NBP2-54789-img0006.jpg)
![Electron Microscopy: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - TEM of Type 1 alpha-Synuclein Pre-formed Fibrils (PFFs) (NBP2-54789)](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Electron-Microscopy-NBP2-54789-img0008.jpg)
![Product Image: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Toxicity results comparing Active Human Recombinant alpha-Synuclein Pre-formed Fibrils (Type 2) and Active Human Recombinant alpha-Synuclein Pre-formed Fibrils (Type 1) (Catalog No. NBP2-54789). Data was graphed after live cell imaging results were obtained using the following procedure: After 8 days in vitro, primary rat mixed cortical neuron cells were washed with 1X PBS and treated with 500 g/ml of Type 1 and Type 2 alpha-Synuclein Proteins for 20 hours at 37C. Following treatements, cells were washed with 2X PBS and incubated with a staining solution (2.0 M Cell Event + 2.5 M Ethidium homodimer + 2.5 g/ml Hoechst 33342 in sterile HBSS) for 30 minutes at 37C. The addition of the Type 2 alpha-Synuclein Proteins resulted in a significant increase in cell death.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Product-Image-NBP2-54789-img0010.jpg)
![Electron Microscopy: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - TEM of Type 1 alpha-Synuclein Pre-formed Fibrils (PFFs) (NBP2-54789)](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Electron-Microscopy-NBP2-54789-img0009.jpg)
![Western Blot: BACE-1 Antibody (3C1C3) [NBP2-37261] - Analysis using BACE1 mouse mAb against Hela (1), SK-N-SH (2), HepG2 (3), C6 (4), PC-12 (5), PANC-1 (6), NIH/3T3 (7) cell lysate.](http://images.novusbio.com/fullsize/BACE-1-Antibody-3C1C3-Western-Blot-NBP2-37261-img0004.jpg "Western Blot: BACE-1 Antibody (3C1C3) [NBP2-37261] - Analysis using BACE1 mouse mAb against Hela (1), SK-N-SH (2), HepG2 (3), C6 (4), PC-12 (5), PANC-1 (6), NIH/3T3 (7) cell lysate.")
![Immunocytochemistry/Immunofluorescence: BACE-1 Antibody (3C1C3) [NBP2-37261] - Analysis of MCF-7 cells using BACE1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/BACE-1-Antibody-3C1C3-Immunocytochemistry-Immunofluorescence-NBP2-37261-img0005.jpg)
![Flow Cytometry: BACE-1 Antibody (3C1C3) [NBP2-37261] - Analysis of Hela cells using BACE1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/BACE-1-Antibody-3C1C3-Flow-Cytometry-NBP2-37261-img0006.jpg)
![Western Blot: BACE-1 Antibody (3C1C3) [NBP2-37261] - Analysis using BACE1 mAb against human BACE1 (AA: 112-324) recombinant protein. (Expected MW is 49.9 kDa)](http://images.novusbio.com/fullsize/BACE-1-Antibody-3C1C3-Western-Blot-NBP2-37261-img0002.jpg)
![Western Blot: BACE-1 Antibody (3C1C3) [NBP2-37261] - Analysis using BACE1 mAb against HEK293 (1) and BACE1 (AA: 112-324)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/BACE-1-Antibody-3C1C3-Western-Blot-NBP2-37261-img0003.jpg)
![ELISA: BACE-1 Antibody (3C1C3) [NBP2-37261] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/BACE-1-Antibody-3C1C3-ELISA-NBP2-37261-img0001.jpg)
![Western Blot: Kv1.5 Antibody [NBP2-76939] - Western blot analysis of KCNA5 on PANC-1 cell lysate using anti-KCNA5 antibody at 1:5000 dilution.](http://images.novusbio.com/fullsize/Kv1.5-Antibody-Western-Blot-NBP2-76939-img0001.jpg "Western Blot: Kv1.5 Antibody [NBP2-76939] - Western blot analysis of KCNA5 on PANC-1 cell lysate using anti-KCNA5 antibody at 1:5000 dilution.")
![Immunocytochemistry/Immunofluorescence: Kv1.5 Antibody [NBP2-76939] - ICC staining KCNA5 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100, PBS.](http://images.novusbio.com/fullsize/Kv1.5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76939-img0004.jpg)
![Immunohistochemistry-Paraffin: Kv1.5 Antibody [NBP2-76939] - Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-KCNA5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Kv1.5-Antibody-Immunohistochemistry-Paraffin-NBP2-76939-img0007.jpg)
![Flow Cytometry: Kv1.5 Antibody [NBP2-76939] - Flow cytometric analysis of PANC-1 cells with KCNA5 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Kv1.5-Antibody-Flow-Cytometry-NBP2-76939-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Kv1.5 Antibody [NBP2-76939] - ICC staining KCNA5 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100, PBS.](http://images.novusbio.com/fullsize/Kv1.5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76939-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Kv1.5 Antibody [NBP2-76939] - ICC staining KCNA5 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100, PBS.](http://images.novusbio.com/fullsize/Kv1.5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76939-img0005.jpg)
![Immunohistochemistry-Paraffin: Kv1.5 Antibody [NBP2-76939] - Rodent model of heart failure post-myocardial infarction. 60X micrograph from infarct region. Antibody at 1:1000. Nuclear counterstain. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Kv1.5-Antibody-Immunohistochemistry-Paraffin-NBP2-76939-img0012.jpg)
![Immunohistochemistry-Paraffin: Kv1.5 Antibody [NBP2-76939] - Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-KCNA5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Kv1.5-Antibody-Immunohistochemistry-Paraffin-NBP2-76939-img0006.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Lane 1: Opal Prestained Molecular Weight Martker. Lane 2: HEK293T Whole Cell Lysate. Lane 3: Mouse Brain Whole Cell Lysate. Lane 4: A-172 Whole Cell Lysate.Load: 10ug/lane. Primary Antibody: Anti-beta Amyloid at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated at 1:70,000 for 30min at RT. Block: BlockOut Buffer. Predicted MW: ~40-50kDa. Observed MW: ~48kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0021.jpg "Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Lane 1: Opal Prestained Molecular Weight Martker. Lane 2: HEK293T Whole Cell Lysate. Lane 3: Mouse Brain Whole Cell Lysate. Lane 4: A-172 Whole Cell Lysate.Load: 10ug/lane. Primary Antibody: Anti-beta Amyloid at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated at 1:70,000 for 30min at RT. Block: BlockOut Buffer. Predicted MW: ~40-50kDa. Observed MW: ~48kDa.")
![Immunocytochemistry/Immunofluorescence: beta Amyloid Antibody [NBP1-78007]](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78007-img0015.jpg)
![Immunohistochemistry: beta Amyloid Antibody [NBP1-78007] - Human Heart (formalin-fixed, paraffin-embedded) stained with beta Amyloid antibody at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-NBP1-78007-img0024.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Mouse Brain (Lane 1) and Mouse Spinal Chord (Lane 2) were run on a 4-20% gradient gel, Blocked in 1% BSA-TBS-T 30 min RT and probed with Rb-a-Beta Amyloid 1:1000 in 1% BSA-TBS-T o/n 4C. Note: observed band higher than predicted molecular weight of 5 kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0010.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Lane 1: HEK293 WCL. Lane 2: Mouse Brain WCL. Lane 3: A-172 WCL. Load: 10.0ug per lane. Primary antibody: Beta Amyloid Antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase Conjugated Goat Anti Rabbit IgG at 1:40,000 for 30 min at RT. Block for 30 min at RT. Note: observed band higher than predicted molecular weight of 5 kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0018.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Marker: Opal Pre-stained ladder. Lane 1: HEK293 lysate. Lane 2: HeLa Lysate.Lane 3: MCF-7 Lysate.Lane 4: Jurkat Lysate.Lane 5: A431 Lysate.Lane 6: LNCaP Lysate.Lane 7: A-172 Lysate.Lane 8: NIH/3T3 Lysate. Load: 35 ug per lane.Primary antibody: beta Amyloid antibody at 1:5,000 for overnight at 4C.Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT.Blocking Buffer: 1% Casein-TTBS for 30 min at RT.Predicted MW: ~40-50 kDa. Observed size: ~48 kDa for beta Amyloid.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0019.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Lane 1: Opal Prestained Molecular Weight Martker. Lane 2: HEK293T Whole Cell Lysate. Lane 3: Mouse Brain Whole Cell Lysate. Lane 4: A-172 Whole Cell Lysate.Lane 5: Daudi Whole Cell Lysate. Load: 10ug/lane. Primary Antibody: Anti-beta Amyloid at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated at 1:70,000 for 30min at RT. Block: BlockOut Buffer. Predicted MW: ~40-50kDa. Observed MW: ~48kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0020.jpg)
![Immunohistochemistry-Paraffin: beta Amyloid Antibody [NBP1-78007] - Human, multi-brain and multi-cancer slides. Within the multi-tumor block, the antibody showed variable levels of nuclear and cytoplasmic staining between individual tumors, with some tumors showing moderate staining. This image shows EGR-1 staining of human ovarian carcinoma.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-Paraffin-NBP1-78007-img0008.jpg)
![Immunohistochemistry: beta Amyloid Antibody [NBP1-78007] - Immunohistochemistical detection of beta Amyloid using beta Amyloid antibody on TG APP23 mouse brain cortex frozen sections. beta Amyloid antibody used at 1/200 and incubated for 2 hours in TBS/BSA/Tween/azide. Fluorescent labelled anti rabbit IgG was then added. Carl Hobbs, King`s College London, United Kingdom](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-NBP1-78007-img0022.jpg)
![Immunohistochemistry: beta Amyloid Antibody [NBP1-78007] - Immunohistochemistry with beta Amyloid antibody showing amyloid beta plaque staining in human Alzheimer’s disease brain at 10x and 20x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval with E1 (Leica Microsystems) retrieval solution for 20 min and then incubated with rabbit beta Amyloid antibody at 1:100 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used at 1:200 dilution to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 3’3-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-NBP1-78007-img0023.jpg)
![ELISA: beta Amyloid Antibody [NBP1-78007] - ELISA results of purified beta Amyloid antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-ELISA-NBP1-78007-img0025.jpg)
![Western Blot: WWOX Antibody [NBP2-47579] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue.](http://images.novusbio.com/fullsize/WWOX-Antibody-Western-Blot-NBP2-47579-img0005.jpg "Western Blot: WWOX Antibody [NBP2-47579] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue.")
![Immunocytochemistry/Immunofluorescence: WWOX Antibody [NBP2-47579] - Staining of human cell line MCF7 shows localization to the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47579-img0006.jpg)
![Immunohistochemistry-Paraffin: WWOX Antibody [NBP2-47579] - Staining of human thyroid gland shows strong cytoplasmic postivity in glandular cells.](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunohistochemistry-Paraffin-NBP2-47579-img0008.jpg)
![Immunohistochemistry-Paraffin: WWOX Antibody [NBP2-47579] - Staining of human skeletal muscle shows no postivity in myoctyes as expected.](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunohistochemistry-Paraffin-NBP2-47579-img0010.jpg)
![Immunohistochemistry-Paraffin: WWOX Antibody [NBP2-47579] - Staining of human kidney shows moderate cytoplasmic postivity in cells in tubules.](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunohistochemistry-Paraffin-NBP2-47579-img0011.jpg)
![Immunohistochemistry-Paraffin: WWOX Antibody [NBP2-47579] - Staining of human cerebellum shows weak to moderate cytoplasmic positivity in cells in molecular layer.](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunohistochemistry-Paraffin-NBP2-47579-img0007.jpg)
![Immunohistochemistry: WWOX Antibody [NBP2-47579] - Staining of human testis shows moderate cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunohistochemistry-NBP2-47579-img0012.jpg)
![Western Blot: DARPP32 [p Thr75] Antibody [NB300-234] - Analysis of rat caudate lysate showing specific immunolabeling of the ~32k DARPP-32 phosphorylated at Thr75 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated (1200 units for 30 min) before being exposed to the Anti-Thr75 DARPP-32. The immunolabeling is completely eliminated by treatment.](http://images.novusbio.com/fullsize/DARPP32-[p-Thr75]-Antibody-Western-Blot-NB300-234-img0003.jpg "Western Blot: DARPP32 [p Thr75] Antibody [NB300-234] - Analysis of rat caudate lysate showing specific immunolabeling of the ~32k DARPP-32 phosphorylated at Thr75 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated (1200 units for 30 min) before being exposed to the Anti-Thr75 DARPP-32. The immunolabeling is completely eliminated by treatment.")
![Western Blot: DARPP32 [p Thr75] Antibody [NB300-234] - Analysis of Rat caudate lysates prepared from caudate slices that had been incubated in the absence and presence of okadaic acid. The labeling by the antibody to DARPP-32 Thr75 is markedly increased by the okadaic acid treatment.](http://images.novusbio.com/fullsize/DARPP32-[p-Thr75]-Antibody-Western-Blot-NB300-234-img0002.jpg)
![Western Blot: RCAN1 Antibody [NBP1-46853] - Analysis of RCAN in human fetal brain](http://images.novusbio.com/fullsize/RCAN1-Antibody-Western-Blot-NBP1-46853-img0003.jpg "Western Blot: RCAN1 Antibody [NBP1-46853] - Analysis of RCAN in human fetal brain")
![Immunohistochemistry: RCAN1 Antibody [NBP1-46853] - Analysis of RCAN1 in human fetal brain](http://images.novusbio.com/fullsize/RCAN1-Antibody-Immunohistochemistry-NBP1-46853-img0004.jpg)
![Western Blot: WWOX Antibody [NBP1-33456] - Rat tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with WWOX antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/WWOX-Antibody-Western-Blot-NBP1-33456-img0007.jpg "Western Blot: WWOX Antibody [NBP1-33456] - Rat tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with WWOX antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: WWOX Antibody [NBP1-33456] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33456-img0003.jpg)
![Immunohistochemistry-Paraffin: WWOX Antibody [NBP1-33456] - WWOX antibody detects WWOX protein at cytosol on mouse skin by immunohistochemical analysis. Sample: Paraffin-embedded mouse skin. WWOX antibody dilution: 1:500.](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunohistochemistry-Paraffin-NBP1-33456-img0009.jpg)
![Western Blot: WWOX Antibody [NBP1-33456] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/WWOX-Antibody-Western-Blot-NBP1-33456-img0004.jpg)
![Western Blot: WWOX Antibody [NBP1-33456] - Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/WWOX-Antibody-Western-Blot-NBP1-33456-img0005.jpg)
![Immunohistochemistry-Paraffin: WWOX Antibody [NBP1-33456] - WWOX antibody detects WWOX protein at cytoplasm on human placenta by immunohistochemical analysis. Sample: Paraffin-embedded placenta. WWOX antibody dilution: 1:100.](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunohistochemistry-Paraffin-NBP1-33456-img0008.jpg)
![Immunoprecipitation: WWOX Antibody [NBP1-33456] - WWOX antibody immunoprecipitates WWOX protein in IP experiments. IP samples: 293T whole cell extract. A. Control with 4 µg of preimmune Rabbit IgG. B. Immunoprecipitation of WWOX protein by 4 µg WWOX antibody. 10 % SDS-PAGE. The immunoprecipitated WWOX protein was detected by WWOX antibody diluted at 1:500.](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunoprecipitation-NBP1-33456-img0006.jpg)
![Western Blot: IRS1 Antibody [NB100-82001] - Analysis of extracts from 293 cells using NB100-82001](http://images.novusbio.com/fullsize/IRS1-Antibody-Western-Blot-NB100-82001-img0002.jpg "Western Blot: IRS1 Antibody [NB100-82001] - Analysis of extracts from 293 cells using NB100-82001")
![Immunocytochemistry/ Immunofluorescence: IRS1 Antibody - BSA Free [NB100-82001] - Immunofluorescence staining of methanol-fixed Hela cells using NB100-82001.](http://images.novusbio.com/fullsize/nb100-82001_rabbit-polyclonal-irs1-antibody-103202523464533.jpg)
![Immunohistochemistry-Paraffin: IRS1 Antibody [NB100-82001] - Analysis of breast carcinoma tissue, using NB100-82001(left) or the same antibody preincubated with blocking peptide(right).](http://images.novusbio.com/fullsize/nb100-82001_rabbit-polyclonal-irs1-antibody-103202523321013.jpg)