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![Western Blot: Filamin A Antibody [NB100-58814] - Detection of Human and Mouse Filamin A on HeLa whole cell lysate using NB100-58814. Filamin A was also immunoprecipitated by rabbit anti-Filamin A antibody NB100-58813.](http://images.novusbio.com/fullsize/Filamin-A-Antibody-Western-Blot-NB100-58814-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Filamin A Antibody [NB100-58814] - Detection of Human and Mouse Filamin A on HeLa whole cell lysate using NB100-58814. Filamin A was also immunoprecipitated by rabbit anti-Filamin A antibody NB100-58813.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: Filamin A Antibody [NB100-58814] - Section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Filamin A used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Filamin-A-Antibody-Immunohistochemistry-Paraffin-NB100-58814-img0005.jpg)
![Western Blot: Filamin A Antibody [NB100-58813] - Detection of Human and Mouse Filamin A on HeLa whole cell lysate using NB100-58813. Filamin A was also immunoprecipitated by rabbit anti-Filamin A antibody NB100-58814.](http://images.novusbio.com/fullsize/Filamin-A-Antibody-Western-Blot-NB100-58813-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Filamin A Antibody [NB100-58813] - Detection of Human and Mouse Filamin A on HeLa whole cell lysate using NB100-58813. Filamin A was also immunoprecipitated by rabbit anti-Filamin A antibody NB100-58814.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry: Filamin A Antibody [NB100-58813] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Filamin A used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Filamin-A-Antibody-Immunohistochemistry-NB100-58813-img0003.jpg)
![Western Blot: PQBP1 Antibody [NBP1-47271] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PQBP1 antibody NBP1-47271 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. PQBP1 was also immunoprecipitated by rabbit anti-PQBP1 antibody NBP1-47270, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/PQBP1-Antibody-Western-Blot-NBP1-47271-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: PQBP1 Antibody [NBP1-47271] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PQBP1 antibody NBP1-47271 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. PQBP1 was also immunoprecipitated by rabbit anti-PQBP1 antibody NBP1-47270, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry: PQBP1 Antibody [NBP1-47271] - Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-PQBP1 used at a dilution of 1:200 (1 ug/ml) Detection: DAB staining using Immunohistochemistry Accessory Kit](http://images.novusbio.com/fullsize/PQBP1-Antibody-Immunohistochemistry-NBP1-47271-img0003.jpg)
![Western Blot: CoCoA Antibody [NBP1-32154] - Sample(30 ug of whole cell lysate) A:HeLa S3 7.5% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/CoCoA-Antibody-Western-Blot-NBP1-32154-img0004.jpg "Western Blot: CoCoA Antibody [NBP1-32154] - Sample(30 ug of whole cell lysate) A:HeLa S3 7.5% SDS PAGE, antibody diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: CoCoA Antibody [NBP1-32154] - Sample: HeLa cells were fixed in iced-cold MeOH for 5 min. Green: CALCOCO1 protein stained by CoCoA antibody, Internal diluted at 1:500. Blue: Hoechst 33343 staining.](http://images.novusbio.com/fullsize/CoCoA-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32154-img0002.jpg)
![Immunohistochemistry-Paraffin: CoCoA Antibody [NBP1-32154] - Paraffin-embedded C2C12 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/CoCoA-Antibody-Immunohistochemistry-Paraffin-NBP1-32154-img0003.jpg)
![Western Blot: USP9x Antibody [NB100-61591] - Detection of Human and Mouse USP9X by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-USP9X antibody NB100-61591 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. USP9X was also immunoprecipitated by rabbit anti-USP9X antibody NB100-61590, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 1 second (A and B).](http://images.novusbio.com/fullsize/USP9x-Antibody-Western-Blot-NB100-61591-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: USP9x Antibody [NB100-61591] - Detection of Human and Mouse USP9X by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-USP9X antibody NB100-61591 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. USP9X was also immunoprecipitated by rabbit anti-USP9X antibody NB100-61590, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 1 second (A and B).")
![Western Blot: PQBP1 Antibody [NBP1-47270] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PQBP1 antibody NBP1-47270 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. PQBP1 was also immunoprecipitated by rabbit anti-PQBP1 antibody NBP1-47271, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/PQBP1-Antibody-Western-Blot-NBP1-47270-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: PQBP1 Antibody [NBP1-47270] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PQBP1 antibody NBP1-47270 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. PQBP1 was also immunoprecipitated by rabbit anti-PQBP1 antibody NBP1-47271, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry: PQBP1 Antibody [NBP1-47270] - Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-PQBP1 used at a dilution of 1:1,000 (1 ug/ml) Detection: DAB staining using Immunohistochemistry Accessory Kit](http://images.novusbio.com/fullsize/PQBP1-Antibody-Immunohistochemistry-NBP1-47270-img0003.jpg)
![Western Blot: S100 alpha 2 Antibody [NBP2-20247] - Sample (30 ug of whole cell lysate) A: A431 15% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/S100-alpha-2-Antibody-Western-Blot-NBP2-20247-img0001.jpg "Western Blot: S100 alpha 2 Antibody [NBP2-20247] - Sample (30 ug of whole cell lysate) A: A431 15% SDS PAGE gel, diluted at 1:10000.")
![Immunohistochemistry-Paraffin: S100A2 Antibody [NBP2-20247] - Human normal kidney. S100A2 antibody [N1C3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/S100A2-Antibody-Immunohistochemistry-Paraffin-NBP2-20247-img0003.jpg)
![S100A2 antibody [N1C3] detects S100A2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde/PBS for 15 min.Green: S100A2 protein stained by S100A2 antibody [N1C3] (NBP2-20247) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-20247_rabbit-polyclonal-s100a2-antibody-immunocytochemistry-immunofluorescence-2611202518351711.jpg)
![Western Blot: CHIP/STUB1 Antibody [NB100-77315] - Detection of Human and Mouse STUB1/CHIP on HeLa whole cell lysate using NB100-77315. STUB1/CHIP was also immunoprecipitated by rabbit anti-STUB1/CHIP antibody NB100-77316.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-Western-Blot-NB100-77315-img0006.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: CHIP/STUB1 Antibody [NB100-77315] - Detection of Human and Mouse STUB1/CHIP on HeLa whole cell lysate using NB100-77315. STUB1/CHIP was also immunoprecipitated by rabbit anti-STUB1/CHIP antibody NB100-77316.")
![Immunohistochemistry-Paraffin: ATG16L1 Antibody [NBP2-15504] - Immunohistochemical analysis of paraffin-embedded U251 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ATG16L1-Antibody-Immunohistochemistry-Paraffin-NBP2-15504-img0002.jpg "Immunohistochemistry-Paraffin: ATG16L1 Antibody [NBP2-15504] - Immunohistochemical analysis of paraffin-embedded U251 xenograft, using antibody at 1:500 dilution.")
![<b>Genetic Strategies Validation. </b>Western Blot: ATG16L1 Antibody [NBP2-15504] - Wild-type (WT) and ATG16L1 knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATG16L1 antibody [N2C1], Internal diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-15504_rabbit-polyclonal-atg16l1-antibody-161020241940578.jpg)
![Immunohistochemistry-Frozen: HEY1 Antibody [NBP2-16818] - Hey1 staining on E11.5 mouse Pharyngeal Mesoderm. Fixed 4% PFA overnight. Blocked with 1% BSA. Primary antibody dilution at 1:100. Secondary antibody at 1:1000, conjugated to Alexa Fluor 488. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/HEY1-Antibody-Immunohistochemistry-Frozen-NBP2-16818-img0005.jpg "Immunohistochemistry-Frozen: HEY1 Antibody [NBP2-16818] - Hey1 staining on E11.5 mouse Pharyngeal Mesoderm. Fixed 4% PFA overnight. Blocked with 1% BSA. Primary antibody dilution at 1:100. Secondary antibody at 1:1000, conjugated to Alexa Fluor 488. IHC-Fr image submitted by a verified customer review.")
![HEY1 antibody detects HEY1 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HEY1 protein stained by HEY1 antibody (NBP2-16818) diluted at 1:2000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-16818_rabbit-polyclonal-hey1-antibody-immunocytochemistry-immunofluorescence-261120251838400.jpg)
![Western Blot: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody [NBP3-41285] - Sample: Recombinant Nicotinic Acetylcholine R alpha 3/CHRNA3, Human.](http://images.novusbio.com/fullsize/nbp3-41285_rabbit-nicotinic-acetylcholine-r-alpha-3-chrna3-pab-61220242117596.jpg "Western Blot: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody [NBP3-41285] - Sample: Recombinant Nicotinic Acetylcholine R alpha 3/CHRNA3, Human.")
![Western Blot: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody [NBP3-41285] - Sample: Mouse Cerebrum lysate; Primary Ab: 5ug/ml NBP3-41285; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41285_rabbit-nicotinic-acetylcholine-r-alpha-3-chrna3-pab-612202421373076.jpg)
![Western Blot: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody [NBP3-41285] - Sample: Mouse Cerebellum lysate; Primary Ab: 5ug/ml NBP3-41285; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41285_rabbit-nicotinic-acetylcholine-r-alpha-3-chrna3-pab-612202421262975.jpg)