| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36770 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59310-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86684-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92722-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05549-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-14193-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37410-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05528-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59374-25ug | |||
![Western Blot: PMP70 Antibody (3V4K3) [NBP3-16348] - Western blot analysis of extracts of various cell lines, using PMP70 Rabbit mAb (NBP3-16348) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/PMP70-Antibody-3V4K3-Western-Blot-NBP3-16348-img0003.jpg "Western Blot: PMP70 Antibody (3V4K3) [NBP3-16348] - Western blot analysis of extracts of various cell lines, using PMP70 Rabbit mAb (NBP3-16348) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: PMP70 Antibody (3V4K3) [NBP3-16348] - Immunofluorescence analysis of NIH-3T3 cells using PMP70 Rabbit mAb (NBP3-16348) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PMP70-Antibody-3V4K3-Immunocytochemistry-Immunofluorescence-NBP3-16348-img0002.jpg)
![Western Blot: PMP70 Antibody (3V4K3) [NBP3-16348] - Western blot analysis of extracts of various cell lines, using PMP70 Rabbit mAb (NBP3-16348) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/PMP70-Antibody-3V4K3-Western-Blot-NBP3-16348-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: PMP70 Antibody (3V4K3) [NBP3-16348] - Immunofluorescence analysis of HeLa cells using PMP70 Rabbit mAb (NBP3-16348) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PMP70-Antibody-3V4K3-Immunocytochemistry-Immunofluorescence-NBP3-16348-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: PMP70 Antibody (3V4K3) [NBP3-16348] - Confocal imaging of C2C12 cells using PMP70 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16348_rabbit-pmp70-mab-3v4k3-52202511402772.jpg)
![Immunocytochemistry/ Immunofluorescence: PMP70 Antibody (3V4K3) [NBP3-16348] - Confocal imaging of HeLa cells using PMP70 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16348_rabbit-pmp70-mab-3v4k3-522025125489.jpg)
![Western Blot: Ankyrin 3 Antibody (S106-20) [NBP2-59310] - Western Blot analysis of Rat brain membrane lysate showing detection of Ankyrin 3 protein using Mouse Anti-Ankyrin 3 Monoclonal Antibody, Clone S106-20 (NBP2-59310). Primary Antibody: Mouse Anti-Ankyrin 3 Monoclonal Antibody (NBP2-59310) at 1:1000.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-S106-20-Western-Blot-NBP2-59310-img0005.jpg "Western Blot: Ankyrin 3 Antibody (S106-20) [NBP2-59310] - Western Blot analysis of Rat brain membrane lysate showing detection of Ankyrin 3 protein using Mouse Anti-Ankyrin 3 Monoclonal Antibody, Clone S106-20 (NBP2-59310). Primary Antibody: Mouse Anti-Ankyrin 3 Monoclonal Antibody (NBP2-59310) at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody (S106-20) [NBP2-59310] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin 3 Monoclonal Antibody, Clone S106-20 (NBP2-59310). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Ankyrin 3 Monoclonal Antibody (NBP2-59310) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Ankyrin 3 Antibody (D) Composite.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-S106-20-Immunocytochemistry-Immunofluorescence-NBP2-59310-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody (S106-20) [NBP2-59310] - Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ankyrin G Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-S106-20-Immunocytochemistry-Immunofluorescence-NBP2-59310-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody (S106-20) [NBP2-59310] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin 3 Monoclonal Antibody, Clone S106-20 (NBP2-59310). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ankyrin 3 Monoclonal Antibody (NBP2-59310) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Ankyrin 3 Antibody. (D) Composite.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-S106-20-Immunocytochemistry-Immunofluorescence-NBP2-59310-img0007.jpg)
![Western Blot: PMP70 Antibody (PSH03-27) [NBP3-32834] - Western blot analysis of PMP70 on different lysates with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: A431 cell lysate (20 ug/Lane) Lane 4: Caco-2 cell lysate (20 ug/Lane) Lane 5: SH-SY5Y cell lysate (20 ug/Lane) Lane 6: Human liver tissue lysate (40 ug/Lane) Lane 7: Mouse liver tissue lysate (40 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 76 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32834) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32834_rabbit-pmp70-mab-psh03-27-288202412461658.jpg "Western Blot: PMP70 Antibody (PSH03-27) [NBP3-32834] - Western blot analysis of PMP70 on different lysates with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: A431 cell lysate (20 ug/Lane) Lane 4: Caco-2 cell lysate (20 ug/Lane) Lane 5: SH-SY5Y cell lysate (20 ug/Lane) Lane 6: Human liver tissue lysate (40 ug/Lane) Lane 7: Mouse liver tissue lysate (40 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 76 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32834) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PMP70 Antibody (PSH03-27) [NBP3-32834] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32834) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32834_rabbit-pmp70-mab-psh03-27-298202414333688.jpg)
![Immunocytochemistry/ Immunofluorescence: PMP70 Antibody (PSH03-27) [NBP3-32834] - Immunocytochemistry analysis of HepG2 cells labeling PMP70 with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32834_rabbit-pmp70-mab-psh03-27-29202410334056.jpg)
![Flow Cytometry: PMP70 Antibody (PSH03-27) [NBP3-32834] - Flow cytometric analysis of NIH/3T3 cells labeling PMP70. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32834, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32834_rabbit-pmp70-mab-psh03-27-2920241637212.jpg)
![Immunohistochemistry-Paraffin: Cav1.3 Antibody [NBP1-86684] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-86684_rabbit-polyclonal-cav1-3-antibody-immunohistochemistry-paraffin-215202473422..jpg "Immunohistochemistry-Paraffin: Cav1.3 Antibody [NBP1-86684] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: Cav1.3 Antibody [NBP1-86684] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/antibody/nbp1-86684_rabbit-polyclonal-cav1-3-antibody-immunohistochemistry-paraffin-215202473516..jpg)
![Western Blot: CABP7 Antibody [NBP2-92722] - Analysis of extracts of various cell lines, using CABP7 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/CABP7-Antibody-Western-Blot-NBP2-92722-img0005.jpg "Western Blot: CABP7 Antibody [NBP2-92722] - Analysis of extracts of various cell lines, using CABP7 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 90s.")
![Immunohistochemistry-Paraffin: CABP7 Antibody [NBP2-92722] - Mouse testis using CABP7 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CABP7-Antibody-Immunohistochemistry-Paraffin-NBP2-92722-img0004.jpg)
![Immunohistochemistry-Paraffin: CABP7 Antibody [NBP2-92722] - Human breast cancer using CABP7 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CABP7-Antibody-Immunohistochemistry-Paraffin-NBP2-92722-img0001.jpg)
![Immunohistochemistry-Paraffin: CABP7 Antibody [NBP2-92722] - Human stomach using CABP7 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol..](http://images.novusbio.com/fullsize/CABP7-Antibody-Immunohistochemistry-Paraffin-NBP2-92722-img0002.jpg)
![Immunohistochemistry-Paraffin: CABP7 Antibody [NBP2-92722] - Mouse kidney using CABP7 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CABP7-Antibody-Immunohistochemistry-Paraffin-NBP2-92722-img0003.jpg)
![Western Blot: Ankyrin 3 Antibody [NBP3-05549] - Western blot analysis of different tissue and cell lysates using Ankyrin 3 Antibody at a dilution of 1:3,000, in green. [1, 6] Protein standard, [2] Rat brain, [3] Cow cortex, [4] HEK293 cells, [5] COS-1 cells, [7] Rat cortex, [8] rat cortex membrane enriched fraction, [9] mouse cortex, [10] mouse cortex membrane enriched fraction. Bands at 60-190kDa represent Ankyrin 3 splice variants and proteolytic fragments. Larger Ankyrin 3 isoforms of 270kDa and 480kDa can be seen on longer exposure of the blots.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-Western-Blot-NBP3-05549-img0002.jpg "Western Blot: Ankyrin 3 Antibody [NBP3-05549] - Western blot analysis of different tissue and cell lysates using Ankyrin 3 Antibody at a dilution of 1:3,000, in green. [1, 6] Protein standard, [2] Rat brain, [3] Cow cortex, [4] HEK293 cells, [5] COS-1 cells, [7] Rat cortex, [8] rat cortex membrane enriched fraction, [9] mouse cortex, [10] mouse cortex membrane enriched fraction. Bands at 60-190kDa represent Ankyrin 3 splice variants and proteolytic fragments. Larger Ankyrin 3 isoforms of 270kDa and 480kDa can be seen on longer exposure of the blots.")
![Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody [NBP3-05549] - Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with Ankyrin 3 Antibody, CPCA-ANK3, at a dilution of 1:2,000 in green, and costained with mouse mAb to RBFOX3/NeuN at a dilution of 1:2,000 in red. The blue is Hoechst staining of nuclear DNA. The Ankyrin 3 antibody stains the axonal initial segments, while the RBFOX3/NeuN antibody reveals perikarya and nuclei of neurons.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05549-img0001.jpg)
![Immunohistochemistry-Paraffin: LIN7C Antibody [NBP2-14193] - Staining of human tonsil shows moderate granular cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/LIN7C-Antibody-Immunohistochemistry-Paraffin-NBP2-14193-img0006.jpg "Immunohistochemistry-Paraffin: LIN7C Antibody [NBP2-14193] - Staining of human tonsil shows moderate granular cytoplasmic positivity in germinal center cells.")
![Immunohistochemistry-Paraffin: LIN7C Antibody [NBP2-14193] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/LIN7C-Antibody-Immunohistochemistry-Paraffin-NBP2-14193-img0001.jpg)
![Immunohistochemistry-Paraffin: LIN7C Antibody [NBP2-14193] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/LIN7C-Antibody-Immunohistochemistry-Paraffin-NBP2-14193-img0002.jpg)
![Immunohistochemistry-Paraffin: LIN7C Antibody [NBP2-14193] - Staining of human placenta shows moderate granular cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/LIN7C-Antibody-Immunohistochemistry-Paraffin-NBP2-14193-img0003.jpg)
![Immunohistochemistry-Paraffin: LIN7C Antibody [NBP2-14193] - Staining of human rectum shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/LIN7C-Antibody-Immunohistochemistry-Paraffin-NBP2-14193-img0004.jpg)
![Immunohistochemistry-Paraffin: LIN7C Antibody [NBP2-14193] - Staining of human thyroid gland shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/LIN7C-Antibody-Immunohistochemistry-Paraffin-NBP2-14193-img0005.jpg)
![Western Blot: Nucleostemin Antibody (2C8D5) [NBP2-37410] - Western blot analysis using GNL3 mouse mAb against NIH3T3 (1) and PC-3 (2) cell lysate.](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-2C8D5-Western-Blot-NBP2-37410-img0004.jpg "Western Blot: Nucleostemin Antibody (2C8D5) [NBP2-37410] - Western blot analysis using GNL3 mouse mAb against NIH3T3 (1) and PC-3 (2) cell lysate.")
![Flow Cytometry: Nucleostemin Antibody (2C8D5) [NBP2-37410] - Flow cytometric analysis of Jurkat cells using GNL3 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-2C8D5-Flow-Cytometry-NBP2-37410-img0005.jpg)
![Western Blot: Nucleostemin Antibody (2C8D5) [NBP2-37410] - Western blot analysis using GNL3 mAb against human GNL3 recombinant protein. (Expected MW is 51.9 kDa)](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-2C8D5-Western-Blot-NBP2-37410-img0002.jpg)
![Western Blot: Nucleostemin Antibody (2C8D5) [NBP2-37410] - Western blot analysis using GNL3 mAb against HEK293 (1) and GNL3 (AA: 1-226)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-2C8D5-Western-Blot-NBP2-37410-img0003.jpg)
![ELISA: Nucleostemin Antibody (2C8D5) [NBP2-37410] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-2C8D5-ELISA-NBP2-37410-img0001.jpg)
![Western Blot: Ankyrin 3 Antibody [NBP3-05528] - Western blot analysis of different tissue and cell lysates using Ankyrin 3 Antibody, at a at a dilution of of 1:2,000 in green: [1] protein standard (red), [2] NIH-3T3, [3] C6, [4] HEK293, [5] HeLa, [6] SH-SY5Y cells, [7] rat brain, and [8] mouse brain lysates. Bands at ~190kDa represent ankyrin 3 splice variants, higher molecular weight bands at 270kDa and 480kDa can be seen on longer exposure. Lower molecular weight bands are likely proteolytic fragments.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-Western-Blot-NBP3-05528-img0002.jpg "Western Blot: Ankyrin 3 Antibody [NBP3-05528] - Western blot analysis of different tissue and cell lysates using Ankyrin 3 Antibody, at a at a dilution of of 1:2,000 in green: [1] protein standard (red), [2] NIH-3T3, [3] C6, [4] HEK293, [5] HeLa, [6] SH-SY5Y cells, [7] rat brain, and [8] mouse brain lysates. Bands at ~190kDa represent ankyrin 3 splice variants, higher molecular weight bands at 270kDa and 480kDa can be seen on longer exposure. Lower molecular weight bands are likely proteolytic fragments.")
![Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody [NBP3-05528] - Immunofluorescent analysis of cortex section of rat brain stained with Ankyrin 3 Antibody at a at a dilution of of 1:2,000 in green and costained with rabbit polyclonal antibody to NF-L at a at a dilution of of 1:5,000 in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The Ankyrin 3 Antibody stains the axonal initial segments, while the NF-L antibody labels dendrites and axons of neuronal cells.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05528-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody [NBP3-05528] - Immunofluorescent analysis of cortex section of rat brain stained with Ankyrin 3 Antibody at a at a dilution of of 1:2,000 in green and costained with rabbit polyclonal antibody to NF-L at a at a dilution of of 1:5,000 in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The Ankyrin 3 Antibody stains the axonal initial segments, while the NF-L antibody labels dendrites and axons of neuronal cells.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05528-img0001.jpg)
![Western Blot: Connexin 26/GJB2 Antibody [NB100-57840] - Staining of Mouse Brain lysate with antibody at 0.01 ug/mL (A), Mouse Heart (B) and Rat Heart (C) lysate with antibody at 0.5 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Connexin-26-GJB2-Antibody-Western-Blot-NB100-57840-img0002.jpg "Western Blot: Connexin 26/GJB2 Antibody [NB100-57840] - Staining of Mouse Brain lysate with antibody at 0.01 ug/mL (A), Mouse Heart (B) and Rat Heart (C) lysate with antibody at 0.5 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
![Flow Cytometry: Connexin 26/GJB2 Antibody [NB100-57840] - Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Connexin-26-GJB2-Antibody-Flow-Cytometry-NB100-57840-img0003.jpg)
![Western Blot: Connexin 26/GJB2 Antibody [NB100-57840] - Staining of Human Colon (A), Heart (B), Prostate (C), Skin (D) and negative control Adipose (E) lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Connexin-26-GJB2-Antibody-Western-Blot-NB100-57840-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin Brain Monoclonal Antibody, Clone S105-13 (NBP2-59374). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Ankyrin Brain Monoclonal Antibody (NBP2-59374) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Ankyrin Brain Antibody (D) Composite.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-S105-13-Immunocytochemistry-Immunofluorescence-NBP2-59374-img0003.jpg "Immunocytochemistry/Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin Brain Monoclonal Antibody, Clone S105-13 (NBP2-59374). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Ankyrin Brain Monoclonal Antibody (NBP2-59374) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Ankyrin Brain Antibody (D) Composite.")
![Immunocytochemistry/Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ankyrin B Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-S105-13-Immunocytochemistry-Immunofluorescence-NBP2-59374-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin Brain Monoclonal Antibody, Clone S105-13 (NBP2-59374). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ankyrin Brain Monoclonal Antibody (NBP2-59374) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-S105-13-Immunocytochemistry-Immunofluorescence-NBP2-59374-img0004.jpg)