| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80861-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-76798SS | |||
| Azide and BSA Free | NBP2-80879 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80862-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27196SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-166 | |||
| BSA Free | NB400-166SS | |||
| BSA Free | NB400-163SS | |||
| Azide and BSA Free | NBP2-80557 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-89182SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83951-25ul | |||
![Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP1-80861] - Staining in human heart muscle and pancreas tissues using anti-UQCRC2 antibody. Corresponding UQCRC2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/UQCRC2-Antibody-Immunohistochemistry-Paraffin-NBP1-80861-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP1-80861] - Staining in human heart muscle and pancreas tissues using anti-UQCRC2 antibody. Corresponding UQCRC2 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP1-80861] - Staining of human cerebral cortex, heart muscle, liver and pancreas using Anti-UQCRC2 antibody NBP1-80861 (A) shows similar protein distribution across tissues to independent antibody NBP1-80862 (B).](http://images.novusbio.com/fullsize/UQCRC2-Antibody-Immunohistochemistry-Paraffin-NBP1-80861-img0013.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: UQCRC2 Antibody [NBP1-80861] - Analysis using Anti-UQCRC2 antibody NBP1-80861 (A) shows similar pattern to independent antibody NBP1-80862 (B).](http://images.novusbio.com/fullsize/UQCRC2-Antibody-Western-Blot-NBP1-80861-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: UQCRC2 Antibody [NBP1-80861] - Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.](http://images.novusbio.com/fullsize/UQCRC2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80861-img0009.jpg)
![Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP1-80861] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/UQCRC2-Antibody-Immunohistochemistry-Paraffin-NBP1-80861-img0016.jpg)
![Western Blot: UQCRC2 Antibody [NBP1-80861] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/UQCRC2-Antibody-Western-Blot-NBP1-80861-img0008.jpg)
![Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP1-80861] - Staining of human heart muscle shows high expression.](http://images.novusbio.com/fullsize/UQCRC2-Antibody-Immunohistochemistry-Paraffin-NBP1-80861-img0010.jpg)
![Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP1-80861] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/UQCRC2-Antibody-Immunohistochemistry-Paraffin-NBP1-80861-img0011.jpg)
![Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP1-80861] - Staining of human liver.](http://images.novusbio.com/fullsize/UQCRC2-Antibody-Immunohistochemistry-Paraffin-NBP1-80861-img0015.jpg)
![Western Blot: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Niemann-Pick Type C1/NPC1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human/Mouse/Rat Niemann-Pick Type C1/NPC1 Monoclonal Antibody (Catalog # MAB10105) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Niemann-Pick Type C1/NPC1 at approximately 170-250 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Knockout-Validated-NBP2-76798-img0002.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Niemann-Pick Type C1/NPC1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human/Mouse/Rat Niemann-Pick Type C1/NPC1 Monoclonal Antibody (Catalog # MAB10105) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Niemann-Pick Type C1/NPC1 at approximately 170-250 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 for 5 minutes. The cells were incubated with anti- Niemann-Pick C1 Antibody at 2 ug/ml for 60 minutes at room temperature and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Immunocytochemistry-Immunofluorescence-NBP2-76798-img0006.jpg)
![Western Blot: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Total protein from human HeLa, Hek293, mouse 3T3 and Rat PC12 cell lines were separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NPC1 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Western-Blot-NBP2-76798-img0005.jpg)
![Immunohistochemistry-Paraffin: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Niemann-Pick Type C1/NPC1 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Rabbit Anti-Human/Mouse/Rat Niemann-Pick Type C1/NPC1 Monoclonal Antibody (NBP2-76798) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to synaptic puncta.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Immunohistochemistry-Paraffin-NBP2-76798-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Niemann-Pick C1 Antibody (1318A) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Immunocytochemistry-Immunofluorescence-NBP2-76798-img0004.jpg)
![Western Blot: UQCRC2 Antibody [NBP1-80862] - Analysis using Anti-UQCRC2 antibody NBP1-80862 (A) shows similar pattern to independent antibody NBP1-80861 (B).](http://images.novusbio.com/fullsize/UQCRC2-Antibody-Western-Blot-NBP1-80862-img0013.jpg "<b>Independent Antibodies Validation. </b>Western Blot: UQCRC2 Antibody [NBP1-80862] - Analysis using Anti-UQCRC2 antibody NBP1-80862 (A) shows similar pattern to independent antibody NBP1-80861 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: UQCRC2 Antibody [NBP1-80862] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.](http://images.novusbio.com/fullsize/UQCRC2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80862-img0008.jpg)
![Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP1-80862] - Staining of human liver.](http://images.novusbio.com/fullsize/UQCRC2-Antibody-Immunohistochemistry-Paraffin-NBP1-80862-img0014.jpg)
![Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP1-80862] - Staining of human kidney shows distinct cytoplasmic positivity with granular pattern in tubule cells.](http://images.novusbio.com/fullsize/UQCRC2-Antibody-Immunohistochemistry-Paraffin-NBP1-80862-img0002.jpg)
![Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP1-80862] - Staining of human heart muscle shows high expression.](http://images.novusbio.com/fullsize/UQCRC2-Antibody-Immunohistochemistry-Paraffin-NBP1-80862-img0009.jpg)
![Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP1-80862] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/UQCRC2-Antibody-Immunohistochemistry-Paraffin-NBP1-80862-img0010.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP1-80862] - Staining in human heart muscle and pancreas tissues using anti-UQCRC2 antibody. Corresponding UQCRC2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/UQCRC2-Antibody-Immunohistochemistry-Paraffin-NBP1-80862-img0011.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP1-80862] - Staining of human cerebral cortex, heart muscle, liver and pancreas using Anti-UQCRC2 antibody NBP1-80862 (A) shows similar protein distribution across tissues to independent antibody NBP1-80861 (B).](http://images.novusbio.com/fullsize/UQCRC2-Antibody-Immunohistochemistry-Paraffin-NBP1-80862-img0012.jpg)
![Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP1-80862] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/UQCRC2-Antibody-Immunohistochemistry-Paraffin-NBP1-80862-img0015.jpg)
![Western Blot: LRH-1/NR5A2 Antibody [NBP2-27196] - Analysis of NR5A2 in HEPG2 lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse NIH3T3 and 4) RAW lysate probed with 3 ug/ml of NR5A2 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/LRH-1-NR5A2-Antibody-Western-Blot-NBP2-27196-img0004.jpg "Western Blot: LRH-1/NR5A2 Antibody [NBP2-27196] - Analysis of NR5A2 in HEPG2 lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse NIH3T3 and 4) RAW lysate probed with 3 ug/ml of NR5A2 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: LRH-1/NR5A2 Antibody [NBP2-27196] - Analysis of NR5A2/LRH1 in human pancreas tissue using NR5A2/LRH1 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/LRH-1-NR5A2-Antibody-Immunohistochemistry-Paraffin-NBP2-27196-img0003.jpg)
![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NBP1-31258] - Paraffin-embedded endometrial, using antibody at 1:150 dilution.](http://images.novusbio.com/fullsize/GPER-GPR30-Antibody-Immunohistochemistry-Paraffin-NBP1-31258-img0009.jpg "Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NBP1-31258] - Paraffin-embedded endometrial, using antibody at 1:150 dilution.")
![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NBP1-31258] - Paraffin-embedded endometrial, using antibody at 1:150 dilution.](http://images.novusbio.com/fullsize/GPER-GPR30-Antibody-Immunohistochemistry-Paraffin-NBP1-31258-img0008.jpg)
![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NBP1-31258] - GPER/GPR30 antibody [C2C3], C-term detects GPER/GPR30 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human colon cancer.GPER/GPR30 stained by GPER/GPR30 antibody [C2C3], C-term (NBP1-31258) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31258_rabbit-polyclonal-gper-gpr30-antibody-91020242001028.jpg)
![Immunocytochemistry/Immunofluorescence: GPER/GPR30 Antibody [NBP1-31258] - DIV11 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GPR30 stained by GPR30 antibody [C2C3], C-term diluted at 1:100.Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [11710] diluted at 1:500.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/antibody/nbp1-31258_rabbit-polyclonal-gper-gpr30-antibody-immunocytochemistry-immunofluorescence-16122024114732..png)
![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NBP1-31258] - Mouse tissues.GPR30 stained by GPR30 antibody [C2C3], C-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/antibody/nbp1-31258_rabbit-polyclonal-gper-gpr30-antibody-immunohistochemistry-paraffin-1612202411499..png)
![<b>Orthogonal Strategies Validation. </b>Boiled and unboiled AsPC-1 whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GPER/GPR30 antibody [C2C3], C-term (NBP1-31258) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody](http://images.novusbio.com/fullsize/nbp1-31258_rabbit-polyclonal-gper-gpr30-antibody-western-blot-1411202518352213.jpg)
![<b>Orthogonal Strategies Validation. </b>Boiled and unboiled various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GPER/GPR30 antibody [C2C3], C-term (NBP1-31258) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp1-31258_rabbit-polyclonal-gper-gpr30-antibody-western-blot-1411202518344912.jpg)
![GPER/GPR30 antibody [C2C3], C-term detects GPER/GPR30 protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.Green: GPER/GPR30 stained by GPER/GPR30 antibody [C2C3], C-term (NBP1-31258) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-31258_rabbit-polyclonal-gper-gpr30-antibody-immunocytochemistry-immunofluorescence-1411202518434418.jpg)
![<b>Orthogonal Strategies Validation. </b>Non-transfected (-) and transfected (+) bolied and unboiled 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GPER/GPR30 antibody [C2C3], C-term (NBP1-31258) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp1-31258_rabbit-polyclonal-gper-gpr30-antibody-western-blot-1411202518344921.jpg)
![GPER/GPR30 antibody detects GPER/GPR30 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GPER/GPR30 stained by GPER/GPR30 antibody (NBP1-31258) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-31258_rabbit-polyclonal-gper-gpr30-antibody-immunocytochemistry-immunofluorescence-141120251841523.jpg)
![Western Blot: Apolipoprotein D Antibody [NBP2-42526] - Analysis of Human Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Apolipoprotein-D-Antibody-Western-Blot-NBP2-42526-img0002.jpg "Western Blot: Apolipoprotein D Antibody [NBP2-42526] - Analysis of Human Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Apolipoprotein D Antibody [NBP2-42526] - Figure 4. ApoA1, ApoD (Apolipoprotein D), and LCAT immunolabeling in cultured keratocytes, dermal fibroblasts, and HepG2 liver cells. Cells were immunolabeled with ApoD (d-f) antibodies (green). DAPI was used to visualize DNA (blue). Anti-ApoD immunolabeled keratocytes (d) and dermal fibroblasts (e), but not HepG2 cells (f). Image collected and cropped by CiteAb from the following publication (www.mdpi.com/2218-273X/9/12/785/htm) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Apolipoprotein-D-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42526-img0005.jpg)
![Immunohistochemistry-Paraffin: Apolipoprotein D Antibody [NBP2-42526] - Staining of paraffin embedded Human Adrenal Gland. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Apolipoprotein-D-Antibody-Immunohistochemistry-Paraffin-NBP2-42526-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Apolipoprotein D Antibody [NBP2-42526] - Immunostaining of apolipoprotein (Apo)A1, ApoD (Apolipoprotein D), and lecithin:cholesterol acyltransferase (LCAT) in the human central cornea. Frozen sections from an individual cornea were incubated at 4 C with either anti-ApoA1 (a) or LCAT (o) antibodies (green). Nuclei were stained blue with DAPI. Antibodies were detected as described in the Materials and Methods. The epithelium, stromal keratocytes, and endothelium showed staining of LCAT (o). Image collected and cropped by CiteAb from the following publication (www.mdpi.com/2218-273X/9/12/785) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Apolipoprotein-D-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42526-img0004.jpg)
![Immunohistochemistry-Paraffin: Apolipoprotein D Antibody [NBP2-42526] - Staining of paraffin embedded Human Kidney. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Apolipoprotein-D-Antibody-Immunohistochemistry-Paraffin-NBP2-42526-img0001.jpg)
![Western Blot: ABCA7 Antibody (7A1-144) [NB400-163] - Total protein from mouse platelets was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/ml anti-ABCA7 in 5% non-fat milk in TBST and detected with an anti-rat HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/ABCA7-Antibody-7A1-144-Western-Blot-NB400-163-img0010.jpg "Western Blot: ABCA7 Antibody (7A1-144) [NB400-163] - Total protein from mouse platelets was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/ml anti-ABCA7 in 5% non-fat milk in TBST and detected with an anti-rat HRP secondary antibody using chemiluminescence.")
![Western Blot: ABCA7 Antibody (7A1-144) [NB400-163] - Analysis using Azide/BSA FREE version of NB400-163. Detection of ABCA7 using NB 400-166 at various dilutions in ABCA7 transfected HeLa cell lysates.](http://images.novusbio.com/fullsize/ABCA7-Antibody-7A1-144-Western-Blot-NB400-163-img0008.jpg)
![Flow Cytometry: ABCA7 Antibody (7A1-144) [NB400-163] - Analysis using Azide/BSA FREE version of NB400-163. Analysis of 6C (red) (negative control HeLa cells) and A7(blue) (ABCA7 expressing HeLa cells) using NB 400-166 at a dilution of 1:3,000.](http://images.novusbio.com/fullsize/ABCA7-Antibody-7A1-144-Flow-Cytometry-NB400-163-img0007.jpg)
![Western Blot: ABCA7 Antibody (7A1-144) [NB400-163] - Detection of ABCA7 using NB 400-163 at various dilutions in ABCA7 transfected HeLa lysates.](http://images.novusbio.com/fullsize/ABCA7-Antibody-7A1-144-Western-Blot-NB400-163-img0006.jpg)
![Flow Cytometry: ABCA7 Antibody (7A1-144) [NB400-163] - FACS analysis of 6C (red) (negative control HeLa cells) and A7 (blue) (ABCA7 expressing HeLa cells) using NB 400-163 at a 1:2,000 dilution.](http://images.novusbio.com/fullsize/ABCA7-Antibody-7A1-144-Flow-Cytometry-NB400-163-img0005.jpg)
![Western Blot: Niemann-Pick type C1 Like-1 Antibody [NBP2-89182] - Total protein from rat liver was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NPC1L1 (NBP2-89182) in 1% BSA-TBST buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/Niemann-Pick-type-C1-Like-1-Antibody-Western-Blot-NBP2-89182-img0003.jpg "Western Blot: Niemann-Pick type C1 Like-1 Antibody [NBP2-89182] - Total protein from rat liver was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NPC1L1 (NBP2-89182) in 1% BSA-TBST buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).")
![Immunocytochemistry/Immunofluorescence: Niemann-Pick type C1 Like-1 Antibody [NBP2-89182] - PC12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Niemann-Pick type C1 Like-1 Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C1-Like-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-89182-img0002.jpg)
![Immunohistochemistry-Paraffin: Niemann-Pick type C1 Like-1 Antibody [NBP2-89182] - Analysis of a FFPE tissue section of human liver using 1:200 dilution of NPC1L1 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This NPC1L1 antibody generated moderate staining of hepatocyte cytoplasm.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C1-Like-1-Antibody-Immunohistochemistry-Paraffin-NBP2-89182-img0001.jpg)
![Western Blot: UQCRC2 Antibody (3E3O8) [NBP3-16390] - Western blot analysis of extracts of various cell lines, using UQCRC2 Rabbit mAb (NBP3-16390) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/UQCRC2-Antibody-3E3O8-Western-Blot-NBP3-16390-img0002.jpg "Western Blot: UQCRC2 Antibody (3E3O8) [NBP3-16390] - Western blot analysis of extracts of various cell lines, using UQCRC2 Rabbit mAb (NBP3-16390) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: UQCRC2 Antibody (3E3O8) [NBP3-16390] - Immunohistochemistry of paraffin-embedded human colon using UQCRC2 Rabbit mAb (NBP3-16390) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/UQCRC2-Antibody-3E3O8-Immunohistochemistry-Paraffin-NBP3-16390-img0001.jpg)
![Western Blot: UQCRC2 Antibody (3E3O8) [NBP3-16390] - Western blot analysis of extracts of Mouse heart, using UQCRC2 Rabbit mAb (NBP3-16390) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/UQCRC2-Antibody-3E3O8-Western-Blot-NBP3-16390-img0003.jpg)
![Immunohistochemistry: UQCRC2 Antibody (3E3O8) [NBP3-16390] - Immunohistochemistry analysis of paraffin-embedded Rat pancreas tissue using UQCRC2 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16390_rabbit-uqcrc2-mab-3e3o8-52202511402742.jpg)
![Immunohistochemistry: UQCRC2 Antibody (3E3O8) [NBP3-16390] - Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using UQCRC2 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16390_rabbit-uqcrc2-mab-3e3o8-52202511361216.jpg)
![Immunohistochemistry: UQCRC2 Antibody (3E3O8) [NBP3-16390] - Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using UQCRC2 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16390_rabbit-uqcrc2-mab-3e3o8-52202511361280.jpg)
![Immunohistochemistry: UQCRC2 Antibody (3E3O8) [NBP3-16390] - Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using UQCRC2 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16390_rabbit-uqcrc2-mab-3e3o8-52202511383771.jpg)
![Immunohistochemistry: UQCRC2 Antibody (3E3O8) [NBP3-16390] - Immunohistochemistry analysis of paraffin-embedded Human liver tissue using UQCRC2 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16390_rabbit-uqcrc2-mab-3e3o8-52202511365158.jpg)
![Immunohistochemistry: UQCRC2 Antibody (3E3O8) [NBP3-16390] - Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using UQCRC2 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16390_rabbit-uqcrc2-mab-3e3o8-52202511365129.jpg)
![Immunohistochemistry: UQCRC2 Antibody (3E3O8) [NBP3-16390] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using UQCRC2 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16390_rabbit-uqcrc2-mab-3e3o8-52202511383796.jpg)
![Immunohistochemistry-Paraffin: PCYT2 Antibody [NBP1-83951] - Staining of human gastrointestinal, kidney, liver and testis using Anti-PCYT2 antibody NBP1-83951 (A) shows similar protein distribution across tissues to independent antibody NBP1-83952 (B).](http://images.novusbio.com/fullsize/PCYT2-Antibody-Immunohistochemistry-Paraffin-NBP1-83951-img0013.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PCYT2 Antibody [NBP1-83951] - Staining of human gastrointestinal, kidney, liver and testis using Anti-PCYT2 antibody NBP1-83951 (A) shows similar protein distribution across tissues to independent antibody NBP1-83952 (B).")
![<b>Independent Antibodies Validation. </b>Western Blot: PCYT2 Antibody [NBP1-83951] - Analysis using Anti-PCYT2 antibody NBP1-83951 (A) shows similar pattern to independent antibody NBP1-83952 (B).](http://images.novusbio.com/fullsize/PCYT2-Antibody-Western-Blot-NBP1-83951-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: PCYT2 Antibody [NBP1-83951] - Immunofluorescent staining of human cell line U-251 MG shows localization to centrosome.](http://images.novusbio.com/fullsize/PCYT2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83951-img0006.jpg)
![Immunohistochemistry-Paraffin: PCYT2 Antibody [NBP1-83951] - Staining of human Small intestine shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PCYT2-Antibody-Immunohistochemistry-Paraffin-NBP1-83951-img0016.jpg)
![Immunohistochemistry-Paraffin: PCYT2 Antibody [NBP1-83951] - Staining of human Kidney shows strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/PCYT2-Antibody-Immunohistochemistry-Paraffin-NBP1-83951-img0014.jpg)
![Immunohistochemistry-Paraffin: PCYT2 Antibody [NBP1-83951] - Staining of human Liver shows strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/PCYT2-Antibody-Immunohistochemistry-Paraffin-NBP1-83951-img0015.jpg)
![Immunohistochemistry-Paraffin: PCYT2 Antibody [NBP1-83951] - Staining of human Testis shows strong granular cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.](http://images.novusbio.com/fullsize/PCYT2-Antibody-Immunohistochemistry-Paraffin-NBP1-83951-img0017.jpg)
![Immunohistochemistry-Paraffin: Kir3.4 Antibody [NBP1-88081] - Analysis in human adrenal gland and skeletal muscle tissues. Corresponding Kir3.4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Kir3.4-Antibody-Immunohistochemistry-Paraffin-NBP1-88081-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Kir3.4 Antibody [NBP1-88081] - Analysis in human adrenal gland and skeletal muscle tissues. Corresponding Kir3.4 RNA-seq data are presented for the same tissues.")
![Immunohistochemistry-Paraffin: Kir3.4 Antibody [NBP1-88081] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Kir3.4-Antibody-Immunohistochemistry-Paraffin-NBP1-88081-img0012.jpg)
![Immunohistochemistry-Paraffin: Kir3.4 Antibody [NBP1-88081] - Staining of human pancreas shows moderate to strong membranous positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/Kir3.4-Antibody-Immunohistochemistry-Paraffin-NBP1-88081-img0011.jpg)
![Immunohistochemistry-Paraffin: Kir3.4 Antibody [NBP1-88081] - Staining of human skin shows no positivity in squamous epithelial cells as expected.](http://images.novusbio.com/fullsize/Kir3.4-Antibody-Immunohistochemistry-Paraffin-NBP1-88081-img0013.jpg)
![Western Blot: LRH-1/NR5A2 Antibody [NBP2-27204] - Analysis of Lrh-1. HEPG2 lysate in the 1) absence and 2) presence of immunizing peptide, 3) RAW lysate probed with 2 ug/ml of Lrh-1 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/LRH-1-NR5A2-Antibody-Western-Blot-NBP2-27204-img0003.jpg "Western Blot: LRH-1/NR5A2 Antibody [NBP2-27204] - Analysis of Lrh-1. HEPG2 lysate in the 1) absence and 2) presence of immunizing peptide, 3) RAW lysate probed with 2 ug/ml of Lrh-1 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Simple Western: LRH-1/NR5A2 Antibody [NBP2-27204] - Simple Western lane view shows a specific band for LRH-1/NR5A2 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/LRH-1-NR5A2-Antibody-Simple-Western-NBP2-27204-img0002.jpg)