| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92713-50uL | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-34117-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18553-25ul | |||
| Azide and BSA Free | NBP3-44382 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05552-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82544-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26573-50ul | |||
![Western Blot: GAP-43 Antibody (1E3) [NBP1-92713] - Western blot of whole rat spinal cord lysates probed with GAP-43 antibody (1E3) antibody to GAP-43 in lane 16 and another similar antibody in lane 17. Dots in middle of strips indicate position of 50kDa and 37kDa protein bands. The strong single band running at about 43kDa corresponds to GAP-43.](http://images.novusbio.com/fullsize/GAP-43-Antibody-1E3-Western-Blot-NBP1-92713-img0002.jpg "Western Blot: GAP-43 Antibody (1E3) [NBP1-92713] - Western blot of whole rat spinal cord lysates probed with GAP-43 antibody (1E3) antibody to GAP-43 in lane 16 and another similar antibody in lane 17. Dots in middle of strips indicate position of 50kDa and 37kDa protein bands. The strong single band running at about 43kDa corresponds to GAP-43.")
![Immunocytochemistry/Immunofluorescence: GAP-43 Antibody (1E3) [NBP1-92713] - Mixed neuronal cultures stained with GAP-43 antibody (1E3) (green), a rabbit antibody to microtubule associated protein 2 (red) and DNA (blue). The GAP-43 antibody stains the plasma membrane of neurons and is particularly concentrated in dendrites.](http://images.novusbio.com/fullsize/GAP-43-Antibody-1E3-Immunocytochemistry-Immunofluorescence-NBP1-92713-img0001.jpg)
![Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody [NBP2-34117] - Staining of human eye, retina.](http://images.novusbio.com/fullsize/VIAAT-SLC32A1-VGAT-Antibody-Immunohistochemistry-Paraffin-NBP2-34117-img0016.jpg "Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody [NBP2-34117] - Staining of human eye, retina.")
![Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody [NBP2-34117] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/VIAAT-SLC32A1-VGAT-Antibody-Immunohistochemistry-Paraffin-NBP2-34117-img0008.jpg)
![Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody [NBP2-34117] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/VIAAT-SLC32A1-VGAT-Antibody-Immunohistochemistry-Paraffin-NBP2-34117-img0009.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody [NBP2-34117] - Staining in human cerebral cortex and pancreas tissues using anti-VGAT antibody. Corresponding VGAT RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/VIAAT-SLC32A1-VGAT-Antibody-Immunohistochemistry-Paraffin-NBP2-34117-img0011.jpg)
![Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody [NBP2-34117] - Staining of human lymph node.](http://images.novusbio.com/fullsize/VIAAT-SLC32A1-VGAT-Antibody-Immunohistochemistry-Paraffin-NBP2-34117-img0012.jpg)
![Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody [NBP2-34117] - Staining of human colon.](http://images.novusbio.com/fullsize/VIAAT-SLC32A1-VGAT-Antibody-Immunohistochemistry-Paraffin-NBP2-34117-img0013.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody [NBP2-34117] - Staining of human cerebral cortex, colon, eye, retina and lymph node using Anti-VIAAT/SLC32A1/VGAT antibody NBP2-34117 (A) shows similar protein distribution across tissues to independent antibody NBP2-34131 (B).](http://images.novusbio.com/fullsize/VIAAT-SLC32A1-VGAT-Antibody-Immunohistochemistry-Paraffin-NBP2-34117-img0015.jpg)
![Immunohistochemistry-Paraffin: L1CAM Antibody (CL12988) [NBP3-18553] - Staining of human kidney shows strong membranous positivity in a subset of cells in tubules.](http://images.novusbio.com/fullsize/L1CAM-Antibody-CL12988-Immunohistochemistry-Paraffin-NBP3-18553-img0006.jpg "Immunohistochemistry-Paraffin: L1CAM Antibody (CL12988) [NBP3-18553] - Staining of human kidney shows strong membranous positivity in a subset of cells in tubules.")
![Immunohistochemistry-Paraffin: L1CAM Antibody (CL12988) [NBP3-18553] - Analysis in human cerebral cortex and testis tissues using NBP3-18553 antibody. Corresponding L1CAM RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/L1CAM-Antibody-CL12988-Immunohistochemistry-Paraffin-NBP3-18553-img0001.jpg)
![Immunohistochemistry-Paraffin: L1CAM Antibody (CL12988) [NBP3-18553] - Staining of human ovarian cancer (clear cell carcinoma) shows moderate membranous positivity in a subset of tumor cells.](http://images.novusbio.com/fullsize/L1CAM-Antibody-CL12988-Immunohistochemistry-Paraffin-NBP3-18553-img0002.jpg)
![Immunohistochemistry-Paraffin: L1CAM Antibody (CL12988) [NBP3-18553] - Staining of human ovarian cancer (papillary carcinoma) shows moderate membranous positivity in a subset of tumor cells.](http://images.novusbio.com/fullsize/L1CAM-Antibody-CL12988-Immunohistochemistry-Paraffin-NBP3-18553-img0003.jpg)
![Immunohistochemistry-Paraffin: L1CAM Antibody (CL12988) [NBP3-18553] - Staining of human cerebral cortex shows moderate positivity in neuropil.](http://images.novusbio.com/fullsize/L1CAM-Antibody-CL12988-Immunohistochemistry-Paraffin-NBP3-18553-img0004.jpg)
![Immunohistochemistry-Paraffin: L1CAM Antibody (CL12988) [NBP3-18553] - Staining of human cerebellum shows strong positivity in neuropil.](http://images.novusbio.com/fullsize/L1CAM-Antibody-CL12988-Immunohistochemistry-Paraffin-NBP3-18553-img0005.jpg)
![Immunohistochemistry-Paraffin: L1CAM Antibody (CL12988) [NBP3-18553] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/L1CAM-Antibody-CL12988-Immunohistochemistry-Paraffin-NBP3-18553-img0007.jpg)
![Western Blot: MAP2 Antibody [NBP3-05552] - Western blot analysis of whole brain lysates using MAP2 Antibody at a dilution of 1:5,000 in green: [1] protein standard (red), [2] rat, and [3] mouse brain lysate. A band at about 280kDa corresponds to the MAP2A and MAP2B proteins.](http://images.novusbio.com/fullsize/MAP2-Antibody-Western-Blot-NBP3-05552-img0002.jpg "Western Blot: MAP2 Antibody [NBP3-05552] - Western blot analysis of whole brain lysates using MAP2 Antibody at a dilution of 1:5,000 in green: [1] protein standard (red), [2] rat, and [3] mouse brain lysate. A band at about 280kDa corresponds to the MAP2A and MAP2B proteins.")
![Immunocytochemistry/Immunofluorescence: MAP2 Antibody [NBP3-05552] - Immunofluorescent analysis of rat brain stem section stained with goat pAb to MAP2 at a dilution of 1:2,000 in red, and costained with mouse mAb to MBP at a dilution of 1:5,000, in green. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. MAP2 Antibody labels MAP2 protein in the perikarya and dendrites of the most neurons, notably motorneurons in the brain stem, and the MBP antibody stains myelin sheath around axons.](http://images.novusbio.com/fullsize/MAP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05552-img0001.jpg)
![Western Blot: Neuropeptide Y Antibody [NBP3-13163] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Neuropeptide Y antibody (NBP3-13163) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Neuropeptide-Y-Antibody-Western-Blot-NBP3-13163-img0004.jpg "Western Blot: Neuropeptide Y Antibody [NBP3-13163] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Neuropeptide Y antibody (NBP3-13163) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Neuropeptide Y Antibody [NBP3-13163] - Neuropeptide Y antibody detects Neuropeptide Y protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Neuropeptide Y stained by Neuropeptide Y antibody (NBP3-13163) diluted at 1:500. Red: Tau, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Neuropeptide-Y-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13163-img0003.jpg)
![Immunohistochemistry-Paraffin: Neuropeptide Y Antibody [NBP3-13163] - Neuropeptide Y antibody detects Neuropeptide Y protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Neuropeptide Y antibody (NBP3-13163) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Neuropeptide-Y-Antibody-Immunohistochemistry-Paraffin-NBP3-13163-img0001.jpg)
![Western Blot: VGLUT2 Antibody [NBP3-13164] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VGLUT2 antibody (NBP3-13164) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/VGLUT2-Antibody-Western-Blot-NBP3-13164-img0001.jpg "Western Blot: VGLUT2 Antibody [NBP3-13164] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VGLUT2 antibody (NBP3-13164) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: VGLUT2 Antibody [NBP3-13164] - VGLUT2 antibody detects VGLUT2 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VGLUT2 stained by VGLUT2 antibody (NBP3-13164) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/VGLUT2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13164-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Plexin A1 Antibody [NBP1-82544] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & cytosol.](http://images.novusbio.com/fullsize/Plexin-A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82544-img0005.jpg "Immunocytochemistry/Immunofluorescence: Plexin A1 Antibody [NBP1-82544] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & cytosol.")
![Immunohistochemistry-Paraffin: Plexin A1 Antibody [NBP1-82544] - Staining of human skeletal muscle shows no positivity in myocytes.](http://images.novusbio.com/fullsize/Plexin-A1-Antibody-Immunohistochemistry-Paraffin-NBP1-82544-img0010.jpg)
![Immunohistochemistry-Paraffin: Plexin A1 Antibody [NBP1-82544] - Staining of human lung shows strong cytoplasmic postivity in macrophages.](http://images.novusbio.com/fullsize/Plexin-A1-Antibody-Immunohistochemistry-Paraffin-NBP1-82544-img0006.jpg)
![Immunohistochemistry-Paraffin: Plexin A1 Antibody [NBP1-82544] - Staining of human cerebellum shows moderate cytoplsmic postivity in Purkinje cells.](http://images.novusbio.com/fullsize/Plexin-A1-Antibody-Immunohistochemistry-Paraffin-NBP1-82544-img0007.jpg)
![Immunohistochemistry-Paraffin: Plexin A1 Antibody [NBP1-82544] - Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Plexin-A1-Antibody-Immunohistochemistry-Paraffin-NBP1-82544-img0008.jpg)
![Immunohistochemistry-Paraffin: Plexin A1 Antibody [NBP1-82544] - Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Plexin-A1-Antibody-Immunohistochemistry-Paraffin-NBP1-82544-img0009.jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (31-1D1) [NB120-2805] - Human kidney tissue.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M2-CHRM2-Antibody-31-1D1-Immunohistochemistry-Paraffin-NB120-2805-img0005.jpg "Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (31-1D1) [NB120-2805] - Human kidney tissue.")
![Western Blot: KCNN2 Antibody [NBP2-84112] - Host: Rabbit. Target Name: KCNN2. Sample Type: Human Fetal Muscle. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/KCNN2-Antibody-Western-Blot-NBP2-84112-img0004.jpg "Western Blot: KCNN2 Antibody [NBP2-84112] - Host: Rabbit. Target Name: KCNN2. Sample Type: Human Fetal Muscle. Antibody Dilution: 1.0ug/ml")
![Immunohistochemistry: KCNN2 Antibody [NBP2-84112] - Sample Type: Rhesus macaque spinal cord. Primary Antibody Dilution: 1:300. Secondary Antibody: Donkey anti Rabbit 488. Secondary Antibody Dilution: 1:500. Color/Signal Descriptions: Green: KCNN2. Gene Name: KCNN2. Submitted by: Timur Mavlyutov, Ph. D., Department of Pharmacology, University of Wisconsin Medical School, 1300 University Avenue, Madison, WI 53706](http://images.novusbio.com/fullsize/KCNN2-Antibody-Immunohistochemistry-NBP2-84112-img0006.jpg)
![Western Blot: KCNN2 Antibody [NBP2-84112] - WB Suggested Anti-KCNN2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Muscle](http://images.novusbio.com/fullsize/KCNN2-Antibody-Western-Blot-NBP2-84112-img0001.jpg)
![Western Blot: KCNN2 Antibody [NBP2-84112] - Host: Rabbit. Target Name: KCNN2. Sample Type: Human Fetal Heart. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/KCNN2-Antibody-Western-Blot-NBP2-84112-img0002.jpg)
![Western Blot: KCNN2 Antibody [NBP2-84112] - Host: Rabbit. Target Name: KCNN2. Sample Type: Human Fetal Lung. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/KCNN2-Antibody-Western-Blot-NBP2-84112-img0003.jpg)
![Western Blot: KCNN2 Antibody [NBP2-84112] - Host: Rabbit. Target: KCNN2. Positive control (+): Human liver (LI). Negative control (-): HeLa (HL). Antibody concentration: 1ug/ml](http://images.novusbio.com/fullsize/KCNN2-Antibody-Western-Blot-NBP2-84112-img0005.jpg)
![Western Blot: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - Western Blot analysis of Human Alpha Synuclein showing detection of alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Monoclonal Antibody, Clone J18 (NBP3-18266). Lane 1: MW ladder. Lane 2: 0.5 ug human alpha synuclein monomer (SPR-321). Lane 3: 2 ug human alpha synuclein monomer (SPR-321). Lane 4: 0.5 ug human alpha synuclein PFFs (SPR-322). Lane 5: 2 ug human alpha synuclein PFFs (SPR-322). Lane 6: 15 ug human Parkinson's Disease brain lysate.. Block: 5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18266) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. NBP3-18266 does not detect unphosphorylated alpha synuclein.](http://images.novusbio.com/fullsize/alpha-Synuclein-p-Ser-129-Antibody-J18-Western-Blot-NBP3-18266-img0004.jpg "Western Blot: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - Western Blot analysis of Human Alpha Synuclein showing detection of alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Monoclonal Antibody, Clone J18 (NBP3-18266). Lane 1: MW ladder. Lane 2: 0.5 ug human alpha synuclein monomer (SPR-321). Lane 3: 2 ug human alpha synuclein monomer (SPR-321). Lane 4: 0.5 ug human alpha synuclein PFFs (SPR-322). Lane 5: 2 ug human alpha synuclein PFFs (SPR-322). Lane 6: 15 ug human Parkinson's Disease brain lysate.. Block: 5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18266) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. NBP3-18266 does not detect unphosphorylated alpha synuclein.")
![Immunohistochemistry: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - C57/BL6 mice were injected with sonicated recombinant mouse alpha synuclein monomers or fibrils at 8 weeks of age. Mice were unilaterally injected in the dorsal striatum (bregma AP + 0.2 mm, L +/1 2.0 mm, V - 3.0 mm) and sacrificed 30 days post-injection. (A) 1.25 uL mouse alpha synuclein monomers. (B) 2.5 uL mouse alpha synuclein monomers. (C) 2.5 ug alpha synuclein PFFs. (C) 5 ug alpha synuclein PFFs Inset: PBS (negative control). Primary antibody: Anti-alpha-Synuclein (NBP3-18266) at 1:10 000. Secondary antibody: anti-rabbit HRP. Mice injected with PFF displayed alpha synuclein staining in the striatum and cortex and contralateral to the injection site.](http://images.novusbio.com/fullsize/alpha-Synuclein-p-Ser-129-Antibody-J18-Immunohistochemistry-NBP3-18266-img0002.jpg)
![Western Blot: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - Western Blot analysis of Mouse Brain showing detection of alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Monoclonal Antibody, Clone J18 (NBP3-18266). Lane 1: MW ladder. Lane 2: Mouse brain. Load: 15 ug. Block: 5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18266) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Multiple bands due to phosphorylation.](http://images.novusbio.com/fullsize/alpha-Synuclein-p-Ser-129-Antibody-J18-Western-Blot-NBP3-18266-img0005.jpg)
![Immunohistochemistry: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - Immunohistochemistry analysis using Mouse Anti-alpha-Synuclein Monoclonal Antibody, Clone J18 (NBP3-18266). C57/BL6 mice were injected with 5 ug sonicated mouse recombinant alpha synuclein PFFs at 8 weeks of age. Mice were unilaterally injected in the dorsal striatum (bregma AP + 0.2 mm, L +/1 2.0 mm, V - 3.0 mm) and sacrificed 30 days post-injection. (A) contralateral cortex. (B) ipsilateral cortex. (C) contralateral striatum. (D) ipsilateral striatum. Primary Antibody: Mouse Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18266) at 1:10000. Secondary Antibody: anti-rabbit HRP. Courtesy of: Porsolt.](http://images.novusbio.com/fullsize/alpha-Synuclein-p-Ser-129-Antibody-J18-Immunohistochemistry-NBP3-18266-img0001.jpg)
![Dot Blot: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - Dot Blot analysis using Rabbit Anti-alpha-Synuclein Monoclonal Antibody, Clone J18 (NBP3-18266). Tissue: alpha synuclein peptide. Primary Antibody: Rabbit Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18266) at 1:500 for 2 hours at RT with shaking . Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking . Phospho peptide sequence: AYEMP-pS-EEGYQ. Non-phospho peptide sequence: AYEMPSEEGYQ. This sequence is the same for human, mouse, and rat.](http://images.novusbio.com/fullsize/alpha-Synuclein-p-Ser-129-Antibody-J18-Dot-Blot-NBP3-18266-img0003.jpg)
![Immunohistochemistry: c-Fos Antibody (14C10) [NBP3-26573] - Image of c-Fos Antibody (14C10) diluted at 1:81 and staining in paraffin-embedded human adrenal gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26573_rabbit-c-fos-mab-14c10-2722024845324.jpg "Immunohistochemistry: c-Fos Antibody (14C10) [NBP3-26573] - Image of c-Fos Antibody (14C10) diluted at 1:81 and staining in paraffin-embedded human adrenal gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Immunohistochemistry: c-Fos Antibody (14C10) [NBP3-26573] - Image of c-Fos Antibody (14C10) diluted at 1:81 and staining in paraffin-embedded human adrenal gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26573_rabbit-c-fos-mab-14c10-2722024845328.jpg)
![Western Blot: c-Fos Antibody (14C10) [NBP3-26573] - Positive Western Blot detected in: HepG2 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate, 293 whole cell lysate, NIH/3T3 whole cell lysate. All lanes: c-Fos Antibody at 0.81ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 41, 29, 37 KDa Observed band size: 62 KDa](http://images.novusbio.com/fullsize/nbp3-26573_rabbit-c-fos-mab-14c10-282202411351611.jpg)
![Immunocytochemistry/Immunofluorescence: c-Fos Antibody (14C10) [NBP3-26573] - Staining of HepG2 cells with c-Fos Antibody (14C10) at 1:27, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26573_rabbit-c-fos-mab-14c10-28220241249368.jpg)
![Flow Cytometry: c-Fos Antibody (14C10) [NBP3-26573] - Overlay histogram showing Hela cells stained with c-Fos Antibody (14C10) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26573_rabbit-c-fos-mab-14c10-282202414183668.jpg)
![Western Blot: PHOX2A Antibody (4F6) [H00000401-M01] - Analysis of PHOX2A expression in transfected 293T cell line by PHOX2A monoclonal antibody (M01), clone 4F6.Lane 1: PHOX2A transfected lysate(29.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PHOX2A-Antibody-4F6-Western-Blot-H00000401-M01-img0010.jpg "Western Blot: PHOX2A Antibody (4F6) [H00000401-M01] - Analysis of PHOX2A expression in transfected 293T cell line by PHOX2A monoclonal antibody (M01), clone 4F6.Lane 1: PHOX2A transfected lysate(29.7 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: PHOX2A Antibody (4F6) [H00000401-M01] - Analysis of monoclonal antibody to PHOX2A on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/PHOX2A-Antibody-4F6-Immunocytochemistry-Immunofluorescence-H00000401-M01-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: PHOX2A Antibody (4F6) [H00000401-M01] - Analysis of PHOX2A over-expressed 293 cell line, cotransfected with PHOX2A Validated Chimera RNAi ( Cat # H00000401-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PHOX2A monoclonal antibody (M01), clone 4F6 (Cat # H00000401-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/PHOX2A-Antibody-4F6-Western-Blot-H00000401-M01-img0008.jpg)
![Western Blot: PHOX2A Antibody (4F6) [H00000401-M01] - PHOX2A monoclonal antibody (M01), clone 4F6 Analysis of PHOX2A expression in PC-12.](http://images.novusbio.com/fullsize/PHOX2A-Antibody-4F6-Western-Blot-H00000401-M01-img0009.jpg)